Spiroplasma eriocheiris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3XHB2|A0A0H3XHB2_9MOLU Dihydrofolate reductase OS=Spiroplasma eriocheiris OX=315358 GN=dfrA PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.44NLLTIMSSLTLITTPIISQVGNIQNATPQEE32 pKa = 4.31LLTPGVDD39 pKa = 3.03NNYY42 pKa = 10.47QIDD45 pKa = 4.2ANWDD49 pKa = 3.43FSSAIPSNNEE59 pKa = 3.7TITINAYY66 pKa = 10.13VDD68 pKa = 3.39WSQWDD73 pKa = 3.54PAKK76 pKa = 10.3PQQTLATITTKK87 pKa = 9.87IDD89 pKa = 3.17KK90 pKa = 9.53TLLQDD95 pKa = 5.45DD96 pKa = 4.29NTLGNLMNYY105 pKa = 9.3LNNYY109 pKa = 8.89YY110 pKa = 8.56PTITAGIKK118 pKa = 10.37APFKK122 pKa = 10.55INPEE126 pKa = 3.75VTQYY130 pKa = 11.33GRR132 pKa = 11.84VLEE135 pKa = 6.38GIYY138 pKa = 10.41DD139 pKa = 3.81WNKK142 pKa = 10.33SVQDD146 pKa = 3.97YY147 pKa = 8.35LTGWKK152 pKa = 9.68EE153 pKa = 4.55SYY155 pKa = 9.5WSLNSPTNPFCAEE168 pKa = 3.91QPNNYY173 pKa = 6.86CTRR176 pKa = 11.84GIDD179 pKa = 4.54GLEE182 pKa = 3.95LQQNDD187 pKa = 3.59TFDD190 pKa = 3.91LYY192 pKa = 10.54FVRR195 pKa = 11.84LVV197 pKa = 3.15

Molecular weight: 22.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3XME8|A0A0H3XME8_9MOLU Uncharacterized protein OS=Spiroplasma eriocheiris OX=315358 GN=SERIO_v1c05120 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.14IKK11 pKa = 10.33HH12 pKa = 5.53KK13 pKa = 9.07RR14 pKa = 11.84THH16 pKa = 5.84GFRR19 pKa = 11.84ARR21 pKa = 11.84MQSEE25 pKa = 4.25SGRR28 pKa = 11.84KK29 pKa = 8.22VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1180

0

1180

390732

33

1766

331.1

37.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.794 ± 0.062

0.745 ± 0.02

5.215 ± 0.055

5.67 ± 0.098

5.037 ± 0.062

5.111 ± 0.067

1.826 ± 0.029

9.605 ± 0.072

8.329 ± 0.078

10.175 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.12 ± 0.029

7.461 ± 0.076

3.21 ± 0.042

4.04 ± 0.042

2.819 ± 0.044

5.668 ± 0.042

5.777 ± 0.054

5.903 ± 0.052

1.159 ± 0.031

4.338 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski