Methanobrevibacter arboriphilus JCM 13429 = DSM 1125

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter; Methanobrevibacter arboriphilus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1961 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V6N415|A0A1V6N415_9EURY Uncharacterized protein OS=Methanobrevibacter arboriphilus JCM 13429 = DSM 1125 OX=1300164 GN=MBBAR_3c00960 PE=4 SV=1
MM1 pKa = 7.77SNGNVKK7 pKa = 9.98GVNFLNNTINVTNGDD22 pKa = 3.06GFYY25 pKa = 10.2FYY27 pKa = 10.65TSGAGVTNVTDD38 pKa = 4.24FVVSGNTIFATKK50 pKa = 10.37AGLNFTGLSSGSLVNVTVEE69 pKa = 3.95YY70 pKa = 10.95NRR72 pKa = 11.84IIASVGVNITGHH84 pKa = 6.3NDD86 pKa = 2.77NSSFDD91 pKa = 4.19RR92 pKa = 11.84NWWGLNNITGKK103 pKa = 10.23ILGIDD108 pKa = 3.77TLNHH112 pKa = 7.05FILNITNTSSLDD124 pKa = 3.49GVHH127 pKa = 6.88FGDD130 pKa = 3.77NVSFMLLVLNTTLSNDD146 pKa = 3.16GVEE149 pKa = 4.32FLPDD153 pKa = 3.61FVVNGTFNGDD163 pKa = 3.63DD164 pKa = 3.97FNSSRR169 pKa = 11.84VDD171 pKa = 3.18GFVYY175 pKa = 10.58NATATAGVQTLAATLDD191 pKa = 3.74NVDD194 pKa = 4.65DD195 pKa = 4.12NVAFXSQLTTNSSIIVSNDD214 pKa = 2.75SVSIGNNVTISGQLANYY231 pKa = 8.69TGITGVNVTVDD242 pKa = 3.34GNLYY246 pKa = 9.75TDD248 pKa = 3.56VSVNCTGGWNFNYY261 pKa = 7.29TTNRR265 pKa = 11.84TGTITVSVNYY275 pKa = 9.99VGNEE279 pKa = 3.66NN280 pKa = 3.54

Molecular weight: 29.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V6N354|A0A1V6N354_9EURY Uncharacterized protein OS=Methanobrevibacter arboriphilus JCM 13429 = DSM 1125 OX=1300164 GN=MBBAR_6c01030 PE=4 SV=1
MM1 pKa = 7.75PEE3 pKa = 3.23SRR5 pKa = 11.84YY6 pKa = 9.84RR7 pKa = 11.84SRR9 pKa = 11.84SYY11 pKa = 11.21KK12 pKa = 10.25RR13 pKa = 11.84INKK16 pKa = 7.17KK17 pKa = 8.43TPGGQSVLRR26 pKa = 11.84YY27 pKa = 9.4KK28 pKa = 10.46KK29 pKa = 10.18KK30 pKa = 10.5KK31 pKa = 8.64PSKK34 pKa = 9.98HH35 pKa = 5.26ICAEE39 pKa = 4.06CGAILHH45 pKa = 6.11GVPRR49 pKa = 11.84GRR51 pKa = 11.84PYY53 pKa = 10.8EE54 pKa = 3.92IGKK57 pKa = 9.98LSKK60 pKa = 9.39TKK62 pKa = 10.17KK63 pKa = 9.59RR64 pKa = 11.84PNRR67 pKa = 11.84PFGGNLCSKK76 pKa = 10.23CARR79 pKa = 11.84DD80 pKa = 3.75YY81 pKa = 10.97FKK83 pKa = 11.37SEE85 pKa = 3.68ARR87 pKa = 11.84SS88 pKa = 3.33

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1961

0

1961

608793

29

3268

310.5

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.262 ± 0.059

1.022 ± 0.031

5.969 ± 0.055

6.724 ± 0.098

4.48 ± 0.05

6.661 ± 0.07

1.416 ± 0.024

10.404 ± 0.064

8.463 ± 0.099

8.399 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.034

8.172 ± 0.134

3.051 ± 0.035

1.949 ± 0.028

2.815 ± 0.037

7.152 ± 0.065

5.28 ± 0.076

6.161 ± 0.061

0.662 ± 0.018

3.787 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski