Amino acid dipepetide frequency for Lactococcus lactis phage p272

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.421AlaAla: 0.421 ± 0.2
0.21AlaCys: 0.21 ± 0.167
2.631AlaAsp: 2.631 ± 0.549
5.052AlaGlu: 5.052 ± 0.867
3.052AlaPhe: 3.052 ± 0.505
3.999AlaGly: 3.999 ± 0.864
0.947AlaHis: 0.947 ± 0.256
4.21AlaIle: 4.21 ± 0.977
5.683AlaLys: 5.683 ± 0.844
5.999AlaLeu: 5.999 ± 0.872
2.21AlaMet: 2.21 ± 0.646
3.999AlaAsn: 3.999 ± 0.88
0.947AlaPro: 0.947 ± 0.347
2.315AlaGln: 2.315 ± 0.452
2.105AlaArg: 2.105 ± 0.412
2.947AlaSer: 2.947 ± 0.731
2.842AlaThr: 2.842 ± 0.67
4.525AlaVal: 4.525 ± 0.969
1.894AlaTrp: 1.894 ± 0.535
2.0AlaTyr: 2.0 ± 0.423
0.0AlaXaa: 0.0 ± 0.0
Cys
0.316CysAla: 0.316 ± 0.16
0.105CysCys: 0.105 ± 0.114
0.316CysAsp: 0.316 ± 0.2
0.21CysGlu: 0.21 ± 0.152
0.105CysPhe: 0.105 ± 0.111
0.526CysGly: 0.526 ± 0.281
0.21CysHis: 0.21 ± 0.164
0.421CysIle: 0.421 ± 0.214
0.737CysLys: 0.737 ± 0.289
0.631CysLeu: 0.631 ± 0.27
0.0CysMet: 0.0 ± 0.0
0.631CysAsn: 0.631 ± 0.281
0.105CysPro: 0.105 ± 0.103
0.316CysGln: 0.316 ± 0.162
0.737CysArg: 0.737 ± 0.359
0.316CysSer: 0.316 ± 0.185
0.421CysThr: 0.421 ± 0.224
0.421CysVal: 0.421 ± 0.184
0.105CysTrp: 0.105 ± 0.111
0.737CysTyr: 0.737 ± 0.284
0.0CysXaa: 0.0 ± 0.0
Asp
1.894AspAla: 1.894 ± 0.511
0.316AspCys: 0.316 ± 0.168
3.262AspAsp: 3.262 ± 0.751
3.999AspGlu: 3.999 ± 0.658
3.683AspPhe: 3.683 ± 0.641
4.104AspGly: 4.104 ± 0.654
0.842AspHis: 0.842 ± 0.273
4.631AspIle: 4.631 ± 0.702
5.157AspLys: 5.157 ± 0.699
5.683AspLeu: 5.683 ± 0.796
0.947AspMet: 0.947 ± 0.258
3.789AspAsn: 3.789 ± 0.664
1.368AspPro: 1.368 ± 0.492
0.421AspGln: 0.421 ± 0.239
2.315AspArg: 2.315 ± 0.514
2.842AspSer: 2.842 ± 0.681
4.315AspThr: 4.315 ± 0.765
3.262AspVal: 3.262 ± 0.721
0.947AspTrp: 0.947 ± 0.303
3.052AspTyr: 3.052 ± 0.5
0.0AspXaa: 0.0 ± 0.0
Glu
3.157GluAla: 3.157 ± 0.7
0.421GluCys: 0.421 ± 0.249
3.157GluAsp: 3.157 ± 0.637
6.63GluGlu: 6.63 ± 1.225
3.894GluPhe: 3.894 ± 0.489
2.421GluGly: 2.421 ± 0.354
1.263GluHis: 1.263 ± 0.429
5.999GluIle: 5.999 ± 0.82
6.63GluLys: 6.63 ± 1.215
9.366GluLeu: 9.366 ± 1.33
2.526GluMet: 2.526 ± 0.577
5.893GluAsn: 5.893 ± 0.693
1.368GluPro: 1.368 ± 0.396
3.683GluGln: 3.683 ± 0.685
3.157GluArg: 3.157 ± 0.584
3.473GluSer: 3.473 ± 0.633
4.631GluThr: 4.631 ± 0.697
4.841GluVal: 4.841 ± 0.859
1.052GluTrp: 1.052 ± 0.348
3.473GluTyr: 3.473 ± 0.712
0.0GluXaa: 0.0 ± 0.0
Phe
3.262PheAla: 3.262 ± 0.663
0.421PheCys: 0.421 ± 0.247
3.473PheAsp: 3.473 ± 0.597
2.421PheGlu: 2.421 ± 0.449
1.894PhePhe: 1.894 ± 0.621
1.789PheGly: 1.789 ± 0.477
0.421PheHis: 0.421 ± 0.238
2.947PheIle: 2.947 ± 0.635
4.525PheLys: 4.525 ± 0.724
2.421PheLeu: 2.421 ± 0.544
1.052PheMet: 1.052 ± 0.358
3.578PheAsn: 3.578 ± 0.977
0.737PhePro: 0.737 ± 0.296
0.947PheGln: 0.947 ± 0.271
0.842PheArg: 0.842 ± 0.259
4.104PheSer: 4.104 ± 0.815
2.736PheThr: 2.736 ± 0.496
2.842PheVal: 2.842 ± 0.51
0.21PheTrp: 0.21 ± 0.132
1.894PheTyr: 1.894 ± 0.449
0.0PheXaa: 0.0 ± 0.0
Gly
3.473GlyAla: 3.473 ± 0.991
0.316GlyCys: 0.316 ± 0.159
3.052GlyAsp: 3.052 ± 0.615
4.42GlyGlu: 4.42 ± 0.587
2.21GlyPhe: 2.21 ± 0.529
3.894GlyGly: 3.894 ± 0.707
0.842GlyHis: 0.842 ± 0.337
4.42GlyIle: 4.42 ± 1.24
6.104GlyLys: 6.104 ± 0.58
5.052GlyLeu: 5.052 ± 0.871
1.052GlyMet: 1.052 ± 0.363
4.21GlyAsn: 4.21 ± 0.698
0.421GlyPro: 0.421 ± 0.191
1.579GlyGln: 1.579 ± 0.42
1.894GlyArg: 1.894 ± 0.332
3.789GlySer: 3.789 ± 0.671
3.894GlyThr: 3.894 ± 1.027
5.262GlyVal: 5.262 ± 1.235
1.263GlyTrp: 1.263 ± 0.322
3.789GlyTyr: 3.789 ± 0.684
0.0GlyXaa: 0.0 ± 0.0
His
0.526HisAla: 0.526 ± 0.204
0.526HisCys: 0.526 ± 0.302
1.263HisAsp: 1.263 ± 0.283
0.526HisGlu: 0.526 ± 0.25
0.526HisPhe: 0.526 ± 0.244
1.263HisGly: 1.263 ± 0.375
0.105HisHis: 0.105 ± 0.102
1.158HisIle: 1.158 ± 0.303
1.052HisLys: 1.052 ± 0.393
1.579HisLeu: 1.579 ± 0.442
0.21HisMet: 0.21 ± 0.191
1.579HisAsn: 1.579 ± 0.492
0.105HisPro: 0.105 ± 0.121
0.421HisGln: 0.421 ± 0.203
0.421HisArg: 0.421 ± 0.206
0.105HisSer: 0.105 ± 0.109
0.842HisThr: 0.842 ± 0.354
0.947HisVal: 0.947 ± 0.351
0.21HisTrp: 0.21 ± 0.234
0.947HisTyr: 0.947 ± 0.365
0.0HisXaa: 0.0 ± 0.0
Ile
4.631IleAla: 4.631 ± 0.771
0.21IleCys: 0.21 ± 0.17
4.736IleAsp: 4.736 ± 0.558
6.314IleGlu: 6.314 ± 0.957
3.052IlePhe: 3.052 ± 0.583
3.473IleGly: 3.473 ± 0.812
1.052IleHis: 1.052 ± 0.334
5.052IleIle: 5.052 ± 0.727
6.42IleLys: 6.42 ± 0.792
5.473IleLeu: 5.473 ± 1.052
1.473IleMet: 1.473 ± 0.347
5.262IleAsn: 5.262 ± 0.669
1.684IlePro: 1.684 ± 0.361
2.105IleGln: 2.105 ± 0.43
1.684IleArg: 1.684 ± 0.468
3.368IleSer: 3.368 ± 0.535
4.946IleThr: 4.946 ± 0.64
4.736IleVal: 4.736 ± 0.694
1.579IleTrp: 1.579 ± 0.45
2.631IleTyr: 2.631 ± 0.463
0.0IleXaa: 0.0 ± 0.0
Lys
6.841LysAla: 6.841 ± 0.877
0.316LysCys: 0.316 ± 0.183
5.473LysAsp: 5.473 ± 0.63
8.945LysGlu: 8.945 ± 1.324
2.315LysPhe: 2.315 ± 0.458
5.893LysGly: 5.893 ± 0.785
1.579LysHis: 1.579 ± 0.46
5.893LysIle: 5.893 ± 0.731
9.156LysLys: 9.156 ± 1.183
7.893LysLeu: 7.893 ± 0.906
3.473LysMet: 3.473 ± 0.486
5.893LysAsn: 5.893 ± 0.802
1.684LysPro: 1.684 ± 0.457
3.368LysGln: 3.368 ± 0.681
4.736LysArg: 4.736 ± 0.775
5.262LysSer: 5.262 ± 0.645
5.052LysThr: 5.052 ± 0.63
5.578LysVal: 5.578 ± 0.658
1.579LysTrp: 1.579 ± 0.343
3.683LysTyr: 3.683 ± 0.604
0.0LysXaa: 0.0 ± 0.0
Leu
4.841LeuAla: 4.841 ± 0.594
0.421LeuCys: 0.421 ± 0.193
4.525LeuAsp: 4.525 ± 0.684
6.209LeuGlu: 6.209 ± 0.943
3.262LeuPhe: 3.262 ± 0.601
4.315LeuGly: 4.315 ± 0.699
1.684LeuHis: 1.684 ± 0.481
6.946LeuIle: 6.946 ± 0.954
8.209LeuLys: 8.209 ± 0.893
7.367LeuLeu: 7.367 ± 1.159
2.105LeuMet: 2.105 ± 0.555
5.578LeuAsn: 5.578 ± 0.928
2.947LeuPro: 2.947 ± 0.597
3.262LeuGln: 3.262 ± 0.545
2.315LeuArg: 2.315 ± 0.527
5.262LeuSer: 5.262 ± 0.794
5.473LeuThr: 5.473 ± 0.732
5.788LeuVal: 5.788 ± 0.695
1.473LeuTrp: 1.473 ± 0.393
4.631LeuTyr: 4.631 ± 0.682
0.0LeuXaa: 0.0 ± 0.0
Met
2.421MetAla: 2.421 ± 0.469
0.105MetCys: 0.105 ± 0.091
1.684MetAsp: 1.684 ± 0.527
1.894MetGlu: 1.894 ± 0.522
0.947MetPhe: 0.947 ± 0.364
1.052MetGly: 1.052 ± 0.332
0.316MetHis: 0.316 ± 0.185
2.21MetIle: 2.21 ± 0.525
3.052MetLys: 3.052 ± 0.561
2.421MetLeu: 2.421 ± 0.662
0.316MetMet: 0.316 ± 0.188
1.579MetAsn: 1.579 ± 0.336
0.526MetPro: 0.526 ± 0.28
1.368MetGln: 1.368 ± 0.326
0.526MetArg: 0.526 ± 0.233
1.158MetSer: 1.158 ± 0.305
1.789MetThr: 1.789 ± 0.496
1.368MetVal: 1.368 ± 0.297
0.21MetTrp: 0.21 ± 0.146
1.052MetTyr: 1.052 ± 0.336
0.0MetXaa: 0.0 ± 0.0
Asn
4.631AsnAla: 4.631 ± 1.196
0.316AsnCys: 0.316 ± 0.212
4.315AsnAsp: 4.315 ± 0.711
5.052AsnGlu: 5.052 ± 0.785
2.21AsnPhe: 2.21 ± 0.575
7.051AsnGly: 7.051 ± 0.901
1.052AsnHis: 1.052 ± 0.295
3.683AsnIle: 3.683 ± 0.619
7.998AsnLys: 7.998 ± 1.293
5.788AsnLeu: 5.788 ± 0.782
1.789AsnMet: 1.789 ± 0.533
4.42AsnAsn: 4.42 ± 0.993
1.789AsnPro: 1.789 ± 0.406
2.105AsnGln: 2.105 ± 0.432
2.105AsnArg: 2.105 ± 0.456
4.946AsnSer: 4.946 ± 0.738
3.578AsnThr: 3.578 ± 0.709
3.473AsnVal: 3.473 ± 0.627
0.947AsnTrp: 0.947 ± 0.366
2.526AsnTyr: 2.526 ± 0.554
0.0AsnXaa: 0.0 ± 0.0
Pro
1.158ProAla: 1.158 ± 0.45
0.105ProCys: 0.105 ± 0.111
1.368ProAsp: 1.368 ± 0.473
1.789ProGlu: 1.789 ± 0.348
0.947ProPhe: 0.947 ± 0.303
0.316ProGly: 0.316 ± 0.176
0.21ProHis: 0.21 ± 0.146
1.473ProIle: 1.473 ± 0.471
1.789ProLys: 1.789 ± 0.529
1.684ProLeu: 1.684 ± 0.478
0.631ProMet: 0.631 ± 0.241
2.421ProAsn: 2.421 ± 0.733
0.842ProPro: 0.842 ± 0.311
0.842ProGln: 0.842 ± 0.338
0.421ProArg: 0.421 ± 0.221
1.158ProSer: 1.158 ± 0.315
2.105ProThr: 2.105 ± 0.42
1.684ProVal: 1.684 ± 0.582
0.421ProTrp: 0.421 ± 0.261
0.421ProTyr: 0.421 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
3.157GlnAla: 3.157 ± 0.6
0.105GlnCys: 0.105 ± 0.094
2.0GlnAsp: 2.0 ± 0.512
2.421GlnGlu: 2.421 ± 0.615
1.473GlnPhe: 1.473 ± 0.331
2.315GlnGly: 2.315 ± 0.436
0.316GlnHis: 0.316 ± 0.179
1.579GlnIle: 1.579 ± 0.326
2.736GlnLys: 2.736 ± 0.576
3.157GlnLeu: 3.157 ± 0.552
1.368GlnMet: 1.368 ± 0.297
2.736GlnAsn: 2.736 ± 0.499
0.737GlnPro: 0.737 ± 0.257
1.368GlnGln: 1.368 ± 0.307
1.368GlnArg: 1.368 ± 0.38
2.0GlnSer: 2.0 ± 0.384
2.0GlnThr: 2.0 ± 0.309
2.0GlnVal: 2.0 ± 0.513
0.631GlnTrp: 0.631 ± 0.202
1.158GlnTyr: 1.158 ± 0.293
0.0GlnXaa: 0.0 ± 0.0
Arg
2.0ArgAla: 2.0 ± 0.658
0.526ArgCys: 0.526 ± 0.214
1.684ArgAsp: 1.684 ± 0.384
2.631ArgGlu: 2.631 ± 0.545
1.052ArgPhe: 1.052 ± 0.34
2.105ArgGly: 2.105 ± 0.422
0.842ArgHis: 0.842 ± 0.278
2.21ArgIle: 2.21 ± 0.455
4.315ArgLys: 4.315 ± 0.772
3.789ArgLeu: 3.789 ± 0.618
0.631ArgMet: 0.631 ± 0.305
2.105ArgAsn: 2.105 ± 0.495
0.421ArgPro: 0.421 ± 0.197
1.789ArgGln: 1.789 ± 0.412
1.789ArgArg: 1.789 ± 0.442
1.789ArgSer: 1.789 ± 0.45
2.21ArgThr: 2.21 ± 0.422
2.21ArgVal: 2.21 ± 0.476
0.105ArgTrp: 0.105 ± 0.106
2.21ArgTyr: 2.21 ± 0.476
0.0ArgXaa: 0.0 ± 0.0
Ser
4.946SerAla: 4.946 ± 1.337
0.842SerCys: 0.842 ± 0.322
3.157SerAsp: 3.157 ± 0.511
3.368SerGlu: 3.368 ± 0.501
3.262SerPhe: 3.262 ± 0.487
5.052SerGly: 5.052 ± 1.428
0.631SerHis: 0.631 ± 0.219
3.894SerIle: 3.894 ± 0.774
5.578SerLys: 5.578 ± 0.821
4.42SerLeu: 4.42 ± 0.604
2.105SerMet: 2.105 ± 0.322
3.894SerAsn: 3.894 ± 0.662
1.052SerPro: 1.052 ± 0.498
1.789SerGln: 1.789 ± 0.416
2.21SerArg: 2.21 ± 0.41
4.736SerSer: 4.736 ± 0.767
2.947SerThr: 2.947 ± 0.614
3.999SerVal: 3.999 ± 0.54
0.526SerTrp: 0.526 ± 0.201
1.684SerTyr: 1.684 ± 0.381
0.0SerXaa: 0.0 ± 0.0
Thr
4.21ThrAla: 4.21 ± 0.618
0.421ThrCys: 0.421 ± 0.239
3.683ThrAsp: 3.683 ± 0.658
4.946ThrGlu: 4.946 ± 0.638
2.421ThrPhe: 2.421 ± 0.591
3.999ThrGly: 3.999 ± 0.459
0.21ThrHis: 0.21 ± 0.129
4.42ThrIle: 4.42 ± 0.694
4.841ThrLys: 4.841 ± 0.652
5.578ThrLeu: 5.578 ± 0.694
1.263ThrMet: 1.263 ± 0.345
3.894ThrAsn: 3.894 ± 0.642
2.0ThrPro: 2.0 ± 0.322
3.052ThrGln: 3.052 ± 0.547
2.421ThrArg: 2.421 ± 0.467
4.21ThrSer: 4.21 ± 0.626
3.262ThrThr: 3.262 ± 0.631
3.578ThrVal: 3.578 ± 0.626
0.737ThrTrp: 0.737 ± 0.243
2.421ThrTyr: 2.421 ± 0.619
0.0ThrXaa: 0.0 ± 0.0
Val
3.999ValAla: 3.999 ± 0.607
0.526ValCys: 0.526 ± 0.294
4.21ValAsp: 4.21 ± 0.653
4.736ValGlu: 4.736 ± 0.621
3.473ValPhe: 3.473 ± 0.634
3.894ValGly: 3.894 ± 0.729
0.737ValHis: 0.737 ± 0.274
4.315ValIle: 4.315 ± 0.575
5.999ValLys: 5.999 ± 0.737
2.736ValLeu: 2.736 ± 0.444
1.894ValMet: 1.894 ± 0.518
3.157ValAsn: 3.157 ± 0.53
1.789ValPro: 1.789 ± 0.55
1.684ValGln: 1.684 ± 0.445
3.262ValArg: 3.262 ± 0.731
5.788ValSer: 5.788 ± 1.279
4.631ValThr: 4.631 ± 0.601
2.631ValVal: 2.631 ± 0.577
0.737ValTrp: 0.737 ± 0.262
2.21ValTyr: 2.21 ± 0.493
0.0ValXaa: 0.0 ± 0.0
Trp
0.526TrpAla: 0.526 ± 0.195
0.421TrpCys: 0.421 ± 0.196
0.842TrpAsp: 0.842 ± 0.41
0.842TrpGlu: 0.842 ± 0.269
0.947TrpPhe: 0.947 ± 0.339
0.737TrpGly: 0.737 ± 0.32
0.105TrpHis: 0.105 ± 0.103
0.631TrpIle: 0.631 ± 0.271
1.263TrpLys: 1.263 ± 0.366
1.579TrpLeu: 1.579 ± 0.416
0.421TrpMet: 0.421 ± 0.204
1.789TrpAsn: 1.789 ± 0.446
0.105TrpPro: 0.105 ± 0.102
0.737TrpGln: 0.737 ± 0.242
0.421TrpArg: 0.421 ± 0.266
1.158TrpSer: 1.158 ± 0.262
0.947TrpThr: 0.947 ± 0.362
0.631TrpVal: 0.631 ± 0.277
0.105TrpTrp: 0.105 ± 0.103
1.052TrpTyr: 1.052 ± 0.32
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.579TyrAla: 1.579 ± 0.461
0.737TyrCys: 0.737 ± 0.372
2.0TyrAsp: 2.0 ± 0.641
4.631TyrGlu: 4.631 ± 0.704
1.789TyrPhe: 1.789 ± 0.378
2.736TyrGly: 2.736 ± 0.495
0.947TyrHis: 0.947 ± 0.313
3.789TyrIle: 3.789 ± 0.702
3.368TyrLys: 3.368 ± 0.69
3.999TyrLeu: 3.999 ± 0.774
0.421TyrMet: 0.421 ± 0.233
3.473TyrAsn: 3.473 ± 0.685
1.158TyrPro: 1.158 ± 0.423
1.473TyrGln: 1.473 ± 0.43
1.684TyrArg: 1.684 ± 0.444
1.684TyrSer: 1.684 ± 0.408
2.947TyrThr: 2.947 ± 0.534
2.631TyrVal: 2.631 ± 0.504
0.526TyrTrp: 0.526 ± 0.246
2.421TyrTyr: 2.421 ± 0.571
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (9503 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski