Amino acid dipepetide frequency for Mycobacterium phage Seagreen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.621AlaAla: 13.621 ± 1.601
1.131AlaCys: 1.131 ± 0.282
6.999AlaAsp: 6.999 ± 0.629
8.022AlaGlu: 8.022 ± 0.763
2.8AlaPhe: 2.8 ± 0.403
10.875AlaGly: 10.875 ± 1.212
2.477AlaHis: 2.477 ± 0.407
3.93AlaIle: 3.93 ± 0.563
4.469AlaLys: 4.469 ± 0.487
8.022AlaLeu: 8.022 ± 0.702
2.746AlaMet: 2.746 ± 0.444
2.746AlaAsn: 2.746 ± 0.439
4.845AlaPro: 4.845 ± 0.52
4.469AlaGln: 4.469 ± 0.446
7.214AlaArg: 7.214 ± 0.708
5.976AlaSer: 5.976 ± 0.655
5.438AlaThr: 5.438 ± 0.628
6.568AlaVal: 6.568 ± 0.601
2.315AlaTrp: 2.315 ± 0.32
2.53AlaTyr: 2.53 ± 0.394
0.0AlaXaa: 0.0 ± 0.0
Cys
1.131CysAla: 1.131 ± 0.363
0.108CysCys: 0.108 ± 0.08
1.292CysAsp: 1.292 ± 0.293
0.861CysGlu: 0.861 ± 0.227
0.054CysPhe: 0.054 ± 0.059
1.723CysGly: 1.723 ± 0.399
0.215CysHis: 0.215 ± 0.111
0.323CysIle: 0.323 ± 0.128
0.323CysLys: 0.323 ± 0.141
0.915CysLeu: 0.915 ± 0.267
0.215CysMet: 0.215 ± 0.102
0.377CysAsn: 0.377 ± 0.158
1.238CysPro: 1.238 ± 0.311
0.377CysGln: 0.377 ± 0.139
0.915CysArg: 0.915 ± 0.26
0.861CysSer: 0.861 ± 0.328
0.7CysThr: 0.7 ± 0.222
0.323CysVal: 0.323 ± 0.147
0.215CysTrp: 0.215 ± 0.125
0.269CysTyr: 0.269 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
7.161AspAla: 7.161 ± 0.632
0.754AspCys: 0.754 ± 0.209
4.415AspAsp: 4.415 ± 0.62
3.661AspGlu: 3.661 ± 0.454
1.615AspPhe: 1.615 ± 0.257
6.676AspGly: 6.676 ± 0.744
1.184AspHis: 1.184 ± 0.251
2.477AspIle: 2.477 ± 0.434
1.454AspLys: 1.454 ± 0.209
6.245AspLeu: 6.245 ± 0.647
1.4AspMet: 1.4 ± 0.283
2.1AspAsn: 2.1 ± 0.388
5.061AspPro: 5.061 ± 0.565
2.154AspGln: 2.154 ± 0.28
5.599AspArg: 5.599 ± 0.646
3.392AspSer: 3.392 ± 0.421
3.769AspThr: 3.769 ± 0.481
4.199AspVal: 4.199 ± 0.533
1.292AspTrp: 1.292 ± 0.251
2.1AspTyr: 2.1 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
6.461GluAla: 6.461 ± 0.691
0.915GluCys: 0.915 ± 0.235
2.961GluAsp: 2.961 ± 0.382
2.584GluGlu: 2.584 ± 0.419
1.723GluPhe: 1.723 ± 0.27
3.5GluGly: 3.5 ± 0.38
1.184GluHis: 1.184 ± 0.302
2.584GluIle: 2.584 ± 0.301
1.992GluLys: 1.992 ± 0.355
5.276GluLeu: 5.276 ± 0.682
1.507GluMet: 1.507 ± 0.334
1.831GluAsn: 1.831 ± 0.253
3.069GluPro: 3.069 ± 0.426
2.853GluGln: 2.853 ± 0.459
4.792GluArg: 4.792 ± 0.545
3.015GluSer: 3.015 ± 0.376
4.092GluThr: 4.092 ± 0.574
4.361GluVal: 4.361 ± 0.569
1.292GluTrp: 1.292 ± 0.294
1.507GluTyr: 1.507 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
2.692PheAla: 2.692 ± 0.41
0.377PheCys: 0.377 ± 0.158
2.154PheAsp: 2.154 ± 0.32
1.669PheGlu: 1.669 ± 0.322
0.915PhePhe: 0.915 ± 0.247
3.338PheGly: 3.338 ± 0.643
0.323PheHis: 0.323 ± 0.12
1.131PheIle: 1.131 ± 0.34
1.292PheLys: 1.292 ± 0.303
1.615PheLeu: 1.615 ± 0.273
0.808PheMet: 0.808 ± 0.202
1.238PheAsn: 1.238 ± 0.357
1.723PhePro: 1.723 ± 0.302
0.969PheGln: 0.969 ± 0.271
1.615PheArg: 1.615 ± 0.276
1.346PheSer: 1.346 ± 0.264
1.992PheThr: 1.992 ± 0.324
1.669PheVal: 1.669 ± 0.302
0.485PheTrp: 0.485 ± 0.168
0.808PheTyr: 0.808 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
10.014GlyAla: 10.014 ± 1.372
1.131GlyCys: 1.131 ± 0.309
7.107GlyAsp: 7.107 ± 0.695
4.146GlyGlu: 4.146 ± 0.491
2.907GlyPhe: 2.907 ± 0.417
10.983GlyGly: 10.983 ± 3.087
1.884GlyHis: 1.884 ± 0.263
4.576GlyIle: 4.576 ± 0.59
2.853GlyLys: 2.853 ± 0.37
5.868GlyLeu: 5.868 ± 0.494
2.1GlyMet: 2.1 ± 0.502
3.123GlyAsn: 3.123 ± 0.374
3.93GlyPro: 3.93 ± 0.663
2.692GlyGln: 2.692 ± 0.507
5.007GlyArg: 5.007 ± 0.602
5.922GlySer: 5.922 ± 0.822
6.245GlyThr: 6.245 ± 0.818
6.461GlyVal: 6.461 ± 0.628
2.746GlyTrp: 2.746 ± 0.397
2.315GlyTyr: 2.315 ± 0.349
0.0GlyXaa: 0.0 ± 0.0
His
1.777HisAla: 1.777 ± 0.352
0.485HisCys: 0.485 ± 0.167
0.969HisAsp: 0.969 ± 0.257
1.346HisGlu: 1.346 ± 0.274
0.538HisPhe: 0.538 ± 0.16
1.615HisGly: 1.615 ± 0.31
1.131HisHis: 1.131 ± 0.244
1.077HisIle: 1.077 ± 0.252
0.915HisLys: 0.915 ± 0.238
1.4HisLeu: 1.4 ± 0.28
0.485HisMet: 0.485 ± 0.142
0.754HisAsn: 0.754 ± 0.201
1.561HisPro: 1.561 ± 0.293
0.485HisGln: 0.485 ± 0.151
2.1HisArg: 2.1 ± 0.345
0.808HisSer: 0.808 ± 0.201
1.615HisThr: 1.615 ± 0.348
1.615HisVal: 1.615 ± 0.282
0.538HisTrp: 0.538 ± 0.183
0.646HisTyr: 0.646 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
5.922IleAla: 5.922 ± 0.521
0.7IleCys: 0.7 ± 0.221
3.553IleAsp: 3.553 ± 0.414
3.176IleGlu: 3.176 ± 0.373
0.969IlePhe: 0.969 ± 0.282
4.038IleGly: 4.038 ± 0.405
1.615IleHis: 1.615 ± 0.36
1.507IleIle: 1.507 ± 0.265
0.969IleLys: 0.969 ± 0.245
2.207IleLeu: 2.207 ± 0.39
0.269IleMet: 0.269 ± 0.119
1.615IleAsn: 1.615 ± 0.275
2.8IlePro: 2.8 ± 0.403
1.4IleGln: 1.4 ± 0.279
2.53IleArg: 2.53 ± 0.411
1.884IleSer: 1.884 ± 0.411
3.338IleThr: 3.338 ± 0.412
3.284IleVal: 3.284 ± 0.394
0.538IleTrp: 0.538 ± 0.196
0.7IleTyr: 0.7 ± 0.155
0.0IleXaa: 0.0 ± 0.0
Lys
3.769LysAla: 3.769 ± 0.488
0.323LysCys: 0.323 ± 0.124
1.615LysAsp: 1.615 ± 0.323
1.777LysGlu: 1.777 ± 0.334
1.077LysPhe: 1.077 ± 0.198
2.692LysGly: 2.692 ± 0.367
0.808LysHis: 0.808 ± 0.202
1.131LysIle: 1.131 ± 0.283
1.454LysLys: 1.454 ± 0.336
2.584LysLeu: 2.584 ± 0.43
0.592LysMet: 0.592 ± 0.206
1.077LysAsn: 1.077 ± 0.247
2.8LysPro: 2.8 ± 0.513
1.346LysGln: 1.346 ± 0.231
1.992LysArg: 1.992 ± 0.41
1.992LysSer: 1.992 ± 0.361
2.315LysThr: 2.315 ± 0.369
2.584LysVal: 2.584 ± 0.419
1.077LysTrp: 1.077 ± 0.289
0.7LysTyr: 0.7 ± 0.215
0.0LysXaa: 0.0 ± 0.0
Leu
8.022LeuAla: 8.022 ± 0.73
0.808LeuCys: 0.808 ± 0.225
5.115LeuAsp: 5.115 ± 0.584
3.661LeuGlu: 3.661 ± 0.434
2.046LeuPhe: 2.046 ± 0.259
5.922LeuGly: 5.922 ± 0.573
0.754LeuHis: 0.754 ± 0.207
3.553LeuIle: 3.553 ± 0.463
2.53LeuLys: 2.53 ± 0.462
5.169LeuLeu: 5.169 ± 0.736
1.292LeuMet: 1.292 ± 0.254
2.584LeuAsn: 2.584 ± 0.379
5.545LeuPro: 5.545 ± 0.752
2.477LeuGln: 2.477 ± 0.43
6.084LeuArg: 6.084 ± 0.75
5.061LeuSer: 5.061 ± 0.519
5.007LeuThr: 5.007 ± 0.483
5.007LeuVal: 5.007 ± 0.488
1.4LeuTrp: 1.4 ± 0.326
2.046LeuTyr: 2.046 ± 0.386
0.0LeuXaa: 0.0 ± 0.0
Met
1.831MetAla: 1.831 ± 0.36
0.108MetCys: 0.108 ± 0.079
1.131MetAsp: 1.131 ± 0.288
1.077MetGlu: 1.077 ± 0.234
0.592MetPhe: 0.592 ± 0.177
2.1MetGly: 2.1 ± 0.319
0.215MetHis: 0.215 ± 0.111
0.7MetIle: 0.7 ± 0.196
0.7MetLys: 0.7 ± 0.195
1.669MetLeu: 1.669 ± 0.273
0.592MetMet: 0.592 ± 0.271
0.915MetAsn: 0.915 ± 0.192
1.454MetPro: 1.454 ± 0.258
0.431MetGln: 0.431 ± 0.152
1.615MetArg: 1.615 ± 0.296
2.638MetSer: 2.638 ± 0.324
2.154MetThr: 2.154 ± 0.33
1.507MetVal: 1.507 ± 0.332
0.377MetTrp: 0.377 ± 0.156
0.162MetTyr: 0.162 ± 0.092
0.0MetXaa: 0.0 ± 0.0
Asn
3.607AsnAla: 3.607 ± 0.429
0.108AsnCys: 0.108 ± 0.07
2.154AsnAsp: 2.154 ± 0.381
2.1AsnGlu: 2.1 ± 0.427
0.808AsnPhe: 0.808 ± 0.268
4.199AsnGly: 4.199 ± 0.463
0.861AsnHis: 0.861 ± 0.182
1.507AsnIle: 1.507 ± 0.406
0.969AsnLys: 0.969 ± 0.235
2.315AsnLeu: 2.315 ± 0.36
0.592AsnMet: 0.592 ± 0.128
1.561AsnAsn: 1.561 ± 0.342
2.53AsnPro: 2.53 ± 0.283
1.131AsnGln: 1.131 ± 0.307
2.154AsnArg: 2.154 ± 0.373
1.561AsnSer: 1.561 ± 0.287
2.207AsnThr: 2.207 ± 0.352
1.831AsnVal: 1.831 ± 0.299
0.485AsnTrp: 0.485 ± 0.145
0.538AsnTyr: 0.538 ± 0.15
0.0AsnXaa: 0.0 ± 0.0
Pro
6.245ProAla: 6.245 ± 0.563
0.7ProCys: 0.7 ± 0.219
4.576ProAsp: 4.576 ± 0.568
4.415ProGlu: 4.415 ± 0.491
1.777ProPhe: 1.777 ± 0.331
6.461ProGly: 6.461 ± 0.778
1.131ProHis: 1.131 ± 0.235
2.261ProIle: 2.261 ± 0.271
2.046ProLys: 2.046 ± 0.475
4.307ProLeu: 4.307 ± 0.465
1.346ProMet: 1.346 ± 0.347
2.261ProAsn: 2.261 ± 0.331
3.769ProPro: 3.769 ± 0.632
2.315ProGln: 2.315 ± 0.388
3.176ProArg: 3.176 ± 0.496
3.5ProSer: 3.5 ± 0.449
3.176ProThr: 3.176 ± 0.396
4.469ProVal: 4.469 ± 0.458
1.023ProTrp: 1.023 ± 0.277
1.831ProTyr: 1.831 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
4.307GlnAla: 4.307 ± 0.665
0.431GlnCys: 0.431 ± 0.19
1.454GlnAsp: 1.454 ± 0.269
1.561GlnGlu: 1.561 ± 0.31
1.077GlnPhe: 1.077 ± 0.263
2.477GlnGly: 2.477 ± 0.443
0.808GlnHis: 0.808 ± 0.238
1.669GlnIle: 1.669 ± 0.281
1.4GlnLys: 1.4 ± 0.236
3.553GlnLeu: 3.553 ± 0.447
0.646GlnMet: 0.646 ± 0.178
1.077GlnAsn: 1.077 ± 0.251
2.207GlnPro: 2.207 ± 0.315
1.669GlnGln: 1.669 ± 0.423
2.477GlnArg: 2.477 ± 0.293
2.638GlnSer: 2.638 ± 0.446
1.992GlnThr: 1.992 ± 0.3
2.154GlnVal: 2.154 ± 0.337
0.915GlnTrp: 0.915 ± 0.24
1.077GlnTyr: 1.077 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
6.353ArgAla: 6.353 ± 0.648
1.131ArgCys: 1.131 ± 0.265
4.899ArgAsp: 4.899 ± 0.649
4.899ArgGlu: 4.899 ± 0.671
1.992ArgPhe: 1.992 ± 0.421
4.684ArgGly: 4.684 ± 0.567
1.507ArgHis: 1.507 ± 0.299
3.823ArgIle: 3.823 ± 0.499
2.369ArgLys: 2.369 ± 0.364
5.007ArgLeu: 5.007 ± 0.55
2.638ArgMet: 2.638 ± 0.437
2.477ArgAsn: 2.477 ± 0.419
3.446ArgPro: 3.446 ± 0.452
2.261ArgGln: 2.261 ± 0.372
5.33ArgArg: 5.33 ± 0.734
3.984ArgSer: 3.984 ± 0.508
3.876ArgThr: 3.876 ± 0.547
5.169ArgVal: 5.169 ± 0.493
1.831ArgTrp: 1.831 ± 0.37
1.777ArgTyr: 1.777 ± 0.262
0.0ArgXaa: 0.0 ± 0.0
Ser
5.761SerAla: 5.761 ± 0.675
0.538SerCys: 0.538 ± 0.175
3.715SerAsp: 3.715 ± 0.409
3.338SerGlu: 3.338 ± 0.411
2.369SerPhe: 2.369 ± 0.506
6.191SerGly: 6.191 ± 0.895
1.454SerHis: 1.454 ± 0.254
2.477SerIle: 2.477 ± 0.399
2.1SerLys: 2.1 ± 0.344
4.092SerLeu: 4.092 ± 0.508
1.454SerMet: 1.454 ± 0.251
1.669SerAsn: 1.669 ± 0.413
3.284SerPro: 3.284 ± 0.372
1.992SerGln: 1.992 ± 0.37
4.253SerArg: 4.253 ± 0.538
3.607SerSer: 3.607 ± 0.666
3.553SerThr: 3.553 ± 0.474
4.253SerVal: 4.253 ± 0.468
1.507SerTrp: 1.507 ± 0.296
1.292SerTyr: 1.292 ± 0.236
0.0SerXaa: 0.0 ± 0.0
Thr
6.191ThrAla: 6.191 ± 0.664
0.7ThrCys: 0.7 ± 0.252
3.661ThrAsp: 3.661 ± 0.529
2.638ThrGlu: 2.638 ± 0.325
1.454ThrPhe: 1.454 ± 0.318
5.976ThrGly: 5.976 ± 0.62
1.615ThrHis: 1.615 ± 0.325
3.338ThrIle: 3.338 ± 0.447
2.154ThrLys: 2.154 ± 0.311
4.792ThrLeu: 4.792 ± 0.516
0.861ThrMet: 0.861 ± 0.206
2.261ThrAsn: 2.261 ± 0.374
4.845ThrPro: 4.845 ± 0.548
1.884ThrGln: 1.884 ± 0.341
4.146ThrArg: 4.146 ± 0.448
3.876ThrSer: 3.876 ± 0.385
4.576ThrThr: 4.576 ± 0.637
5.976ThrVal: 5.976 ± 0.693
1.292ThrTrp: 1.292 ± 0.289
1.723ThrTyr: 1.723 ± 0.292
0.0ThrXaa: 0.0 ± 0.0
Val
7.699ValAla: 7.699 ± 0.542
1.292ValCys: 1.292 ± 0.259
5.33ValAsp: 5.33 ± 0.624
3.823ValGlu: 3.823 ± 0.515
2.1ValPhe: 2.1 ± 0.317
5.815ValGly: 5.815 ± 0.73
1.615ValHis: 1.615 ± 0.302
2.961ValIle: 2.961 ± 0.457
2.423ValLys: 2.423 ± 0.465
5.815ValLeu: 5.815 ± 0.685
1.238ValMet: 1.238 ± 0.227
2.369ValAsn: 2.369 ± 0.353
4.092ValPro: 4.092 ± 0.374
2.746ValGln: 2.746 ± 0.367
4.199ValArg: 4.199 ± 0.467
4.522ValSer: 4.522 ± 0.513
4.792ValThr: 4.792 ± 0.486
6.407ValVal: 6.407 ± 0.641
1.777ValTrp: 1.777 ± 0.349
1.346ValTyr: 1.346 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
2.046TrpAla: 2.046 ± 0.279
0.269TrpCys: 0.269 ± 0.134
1.669TrpAsp: 1.669 ± 0.31
0.969TrpGlu: 0.969 ± 0.276
0.7TrpPhe: 0.7 ± 0.189
0.754TrpGly: 0.754 ± 0.197
0.754TrpHis: 0.754 ± 0.227
0.969TrpIle: 0.969 ± 0.176
0.592TrpLys: 0.592 ± 0.157
1.669TrpLeu: 1.669 ± 0.348
0.861TrpMet: 0.861 ± 0.236
0.538TrpAsn: 0.538 ± 0.194
1.346TrpPro: 1.346 ± 0.273
1.131TrpGln: 1.131 ± 0.234
1.938TrpArg: 1.938 ± 0.36
1.292TrpSer: 1.292 ± 0.267
1.454TrpThr: 1.454 ± 0.31
2.207TrpVal: 2.207 ± 0.481
0.808TrpTrp: 0.808 ± 0.192
0.538TrpTyr: 0.538 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.584TyrAla: 2.584 ± 0.401
0.431TyrCys: 0.431 ± 0.171
1.938TyrAsp: 1.938 ± 0.415
1.777TyrGlu: 1.777 ± 0.332
0.646TyrPhe: 0.646 ± 0.177
1.992TyrGly: 1.992 ± 0.451
0.323TyrHis: 0.323 ± 0.107
1.023TyrIle: 1.023 ± 0.216
0.7TyrLys: 0.7 ± 0.176
1.561TyrLeu: 1.561 ± 0.305
0.269TyrMet: 0.269 ± 0.126
0.646TyrAsn: 0.646 ± 0.178
1.238TyrPro: 1.238 ± 0.222
0.808TyrGln: 0.808 ± 0.207
2.369TyrArg: 2.369 ± 0.408
1.023TyrSer: 1.023 ± 0.224
1.669TyrThr: 1.669 ± 0.376
2.315TyrVal: 2.315 ± 0.323
0.592TyrTrp: 0.592 ± 0.152
0.7TyrTyr: 0.7 ± 0.189
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (18575 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski