Amino acid dipepetide frequency for Streptomyces sp. TLI_235

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
26.107AlaAla: 26.107 ± 0.184
1.113AlaCys: 1.113 ± 0.022
9.204AlaAsp: 9.204 ± 0.074
9.425AlaGlu: 9.425 ± 0.104
3.529AlaPhe: 3.529 ± 0.039
14.651AlaGly: 14.651 ± 0.099
2.941AlaHis: 2.941 ± 0.039
3.266AlaIle: 3.266 ± 0.045
2.616AlaLys: 2.616 ± 0.044
15.166AlaLeu: 15.166 ± 0.105
2.427AlaMet: 2.427 ± 0.033
2.046AlaAsn: 2.046 ± 0.037
8.136AlaPro: 8.136 ± 0.076
3.794AlaGln: 3.794 ± 0.045
10.696AlaArg: 10.696 ± 0.086
5.546AlaSer: 5.546 ± 0.056
7.25AlaThr: 7.25 ± 0.063
13.405AlaVal: 13.405 ± 0.102
2.003AlaTrp: 2.003 ± 0.03
2.523AlaTyr: 2.523 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
1.118CysAla: 1.118 ± 0.024
0.121CysCys: 0.121 ± 0.007
0.47CysAsp: 0.47 ± 0.015
0.373CysGlu: 0.373 ± 0.012
0.216CysPhe: 0.216 ± 0.009
0.996CysGly: 0.996 ± 0.023
0.201CysHis: 0.201 ± 0.009
0.179CysIle: 0.179 ± 0.008
0.095CysLys: 0.095 ± 0.006
0.824CysLeu: 0.824 ± 0.02
0.129CysMet: 0.129 ± 0.006
0.141CysAsn: 0.141 ± 0.008
0.561CysPro: 0.561 ± 0.017
0.18CysGln: 0.18 ± 0.009
0.666CysArg: 0.666 ± 0.016
0.467CysSer: 0.467 ± 0.014
0.584CysThr: 0.584 ± 0.015
0.548CysVal: 0.548 ± 0.015
0.147CysTrp: 0.147 ± 0.008
0.158CysTyr: 0.158 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.341AspAla: 7.341 ± 0.065
0.442AspCys: 0.442 ± 0.015
3.063AspAsp: 3.063 ± 0.038
3.497AspGlu: 3.497 ± 0.04
1.505AspPhe: 1.505 ± 0.025
6.63AspGly: 6.63 ± 0.058
1.485AspHis: 1.485 ± 0.025
1.626AspIle: 1.626 ± 0.029
0.906AspLys: 0.906 ± 0.023
6.603AspLeu: 6.603 ± 0.057
0.711AspMet: 0.711 ± 0.017
0.924AspAsn: 0.924 ± 0.018
4.682AspPro: 4.682 ± 0.046
1.521AspGln: 1.521 ± 0.028
5.285AspArg: 5.285 ± 0.054
2.368AspSer: 2.368 ± 0.034
3.372AspThr: 3.372 ± 0.036
4.078AspVal: 4.078 ± 0.045
1.022AspTrp: 1.022 ± 0.021
1.065AspTyr: 1.065 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.149GluAla: 7.149 ± 0.074
0.352GluCys: 0.352 ± 0.011
2.657GluAsp: 2.657 ± 0.035
3.2GluGlu: 3.2 ± 0.047
1.372GluPhe: 1.372 ± 0.019
3.809GluGly: 3.809 ± 0.038
1.61GluHis: 1.61 ± 0.026
2.086GluIle: 2.086 ± 0.034
1.078GluLys: 1.078 ± 0.025
6.763GluLeu: 6.763 ± 0.067
0.732GluMet: 0.732 ± 0.019
0.914GluAsn: 0.914 ± 0.02
3.408GluPro: 3.408 ± 0.043
2.467GluGln: 2.467 ± 0.04
5.023GluArg: 5.023 ± 0.054
2.29GluSer: 2.29 ± 0.038
2.604GluThr: 2.604 ± 0.034
4.212GluVal: 4.212 ± 0.053
0.686GluTrp: 0.686 ± 0.017
1.031GluTyr: 1.031 ± 0.018
0.0GluXaa: 0.0 ± 0.0
Phe
3.667PheAla: 3.667 ± 0.039
0.278PheCys: 0.278 ± 0.01
1.894PheAsp: 1.894 ± 0.032
1.315PheGlu: 1.315 ± 0.025
0.796PhePhe: 0.796 ± 0.021
2.928PheGly: 2.928 ± 0.035
0.608PheHis: 0.608 ± 0.019
0.591PheIle: 0.591 ± 0.016
0.442PheLys: 0.442 ± 0.015
2.408PheLeu: 2.408 ± 0.029
0.332PheMet: 0.332 ± 0.012
0.534PheAsn: 0.534 ± 0.015
1.323PhePro: 1.323 ± 0.025
0.633PheGln: 0.633 ± 0.016
1.813PheArg: 1.813 ± 0.028
1.286PheSer: 1.286 ± 0.024
1.965PheThr: 1.965 ± 0.029
2.001PheVal: 2.001 ± 0.029
0.399PheTrp: 0.399 ± 0.012
0.526PheTyr: 0.526 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
11.515GlyAla: 11.515 ± 0.078
0.899GlyCys: 0.899 ± 0.023
4.874GlyAsp: 4.874 ± 0.048
4.811GlyGlu: 4.811 ± 0.054
2.784GlyPhe: 2.784 ± 0.033
9.328GlyGly: 9.328 ± 0.102
2.421GlyHis: 2.421 ± 0.034
3.235GlyIle: 3.235 ± 0.042
2.018GlyLys: 2.018 ± 0.037
9.987GlyLeu: 9.987 ± 0.078
1.868GlyMet: 1.868 ± 0.026
1.759GlyAsn: 1.759 ± 0.036
5.957GlyPro: 5.957 ± 0.063
2.716GlyGln: 2.716 ± 0.038
8.686GlyArg: 8.686 ± 0.07
5.434GlySer: 5.434 ± 0.052
6.686GlyThr: 6.686 ± 0.065
7.084GlyVal: 7.084 ± 0.066
1.837GlyTrp: 1.837 ± 0.028
2.302GlyTyr: 2.302 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.757HisAla: 2.757 ± 0.041
0.228HisCys: 0.228 ± 0.009
1.322HisAsp: 1.322 ± 0.022
1.098HisGlu: 1.098 ± 0.026
0.605HisPhe: 0.605 ± 0.016
2.554HisGly: 2.554 ± 0.03
0.751HisHis: 0.751 ± 0.019
0.611HisIle: 0.611 ± 0.017
0.296HisLys: 0.296 ± 0.011
2.63HisLeu: 2.63 ± 0.037
0.295HisMet: 0.295 ± 0.01
0.382HisAsn: 0.382 ± 0.013
2.004HisPro: 2.004 ± 0.032
0.668HisGln: 0.668 ± 0.017
2.511HisArg: 2.511 ± 0.035
0.967HisSer: 0.967 ± 0.025
1.374HisThr: 1.374 ± 0.025
1.546HisVal: 1.546 ± 0.027
0.435HisTrp: 0.435 ± 0.013
0.463HisTyr: 0.463 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.662IleAla: 4.662 ± 0.041
0.283IleCys: 0.283 ± 0.011
2.112IleAsp: 2.112 ± 0.031
1.709IleGlu: 1.709 ± 0.03
0.603IlePhe: 0.603 ± 0.017
3.5IleGly: 3.5 ± 0.043
0.566IleHis: 0.566 ± 0.015
0.684IleIle: 0.684 ± 0.018
0.604IleLys: 0.604 ± 0.018
2.136IleLeu: 2.136 ± 0.034
0.374IleMet: 0.374 ± 0.015
0.659IleAsn: 0.659 ± 0.018
1.62IlePro: 1.62 ± 0.029
0.645IleGln: 0.645 ± 0.017
2.141IleArg: 2.141 ± 0.032
1.501IleSer: 1.501 ± 0.028
2.122IleThr: 2.122 ± 0.032
2.432IleVal: 2.432 ± 0.033
0.376IleTrp: 0.376 ± 0.014
0.452IleTyr: 0.452 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
2.67LysAla: 2.67 ± 0.044
0.108LysCys: 0.108 ± 0.007
1.068LysAsp: 1.068 ± 0.026
0.915LysGlu: 0.915 ± 0.022
0.379LysPhe: 0.379 ± 0.013
1.418LysGly: 1.418 ± 0.031
0.394LysHis: 0.394 ± 0.012
0.732LysIle: 0.732 ± 0.02
0.555LysLys: 0.555 ± 0.022
1.675LysLeu: 1.675 ± 0.031
0.29LysMet: 0.29 ± 0.012
0.44LysAsn: 0.44 ± 0.015
1.206LysPro: 1.206 ± 0.026
0.62LysGln: 0.62 ± 0.017
1.175LysArg: 1.175 ± 0.025
0.956LysSer: 0.956 ± 0.023
1.12LysThr: 1.12 ± 0.028
1.632LysVal: 1.632 ± 0.031
0.224LysTrp: 0.224 ± 0.01
0.384LysTyr: 0.384 ± 0.012
0.0LysXaa: 0.0 ± 0.0
Leu
16.942LeuAla: 16.942 ± 0.113
0.864LeuCys: 0.864 ± 0.017
6.712LeuAsp: 6.712 ± 0.06
4.421LeuGlu: 4.421 ± 0.045
2.48LeuPhe: 2.48 ± 0.037
9.595LeuGly: 9.595 ± 0.071
2.484LeuHis: 2.484 ± 0.041
3.016LeuIle: 3.016 ± 0.036
1.706LeuLys: 1.706 ± 0.034
11.965LeuLeu: 11.965 ± 0.112
1.477LeuMet: 1.477 ± 0.024
1.674LeuAsn: 1.674 ± 0.026
7.131LeuPro: 7.131 ± 0.061
2.451LeuGln: 2.451 ± 0.033
8.857LeuArg: 8.857 ± 0.075
4.822LeuSer: 4.822 ± 0.043
6.808LeuThr: 6.808 ± 0.057
8.945LeuVal: 8.945 ± 0.073
1.295LeuTrp: 1.295 ± 0.028
1.759LeuTyr: 1.759 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.184MetAla: 2.184 ± 0.038
0.114MetCys: 0.114 ± 0.007
0.788MetAsp: 0.788 ± 0.017
0.622MetGlu: 0.622 ± 0.014
0.402MetPhe: 0.402 ± 0.014
1.15MetGly: 1.15 ± 0.025
0.353MetHis: 0.353 ± 0.011
0.566MetIle: 0.566 ± 0.017
0.334MetLys: 0.334 ± 0.013
1.591MetLeu: 1.591 ± 0.026
0.242MetMet: 0.242 ± 0.01
0.394MetAsn: 0.394 ± 0.011
1.078MetPro: 1.078 ± 0.019
0.408MetGln: 0.408 ± 0.013
1.262MetArg: 1.262 ± 0.023
1.184MetSer: 1.184 ± 0.022
1.473MetThr: 1.473 ± 0.025
1.153MetVal: 1.153 ± 0.024
0.18MetTrp: 0.18 ± 0.009
0.261MetTyr: 0.261 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.171AsnAla: 2.171 ± 0.029
0.178AsnCys: 0.178 ± 0.008
0.902AsnAsp: 0.902 ± 0.019
0.757AsnGlu: 0.757 ± 0.018
0.46AsnPhe: 0.46 ± 0.012
1.964AsnGly: 1.964 ± 0.036
0.406AsnHis: 0.406 ± 0.013
0.578AsnIle: 0.578 ± 0.017
0.325AsnLys: 0.325 ± 0.012
1.721AsnLeu: 1.721 ± 0.03
0.257AsnMet: 0.257 ± 0.01
0.442AsnAsn: 0.442 ± 0.017
1.387AsnPro: 1.387 ± 0.028
0.521AsnGln: 0.521 ± 0.016
1.287AsnArg: 1.287 ± 0.025
0.931AsnSer: 0.931 ± 0.024
1.159AsnThr: 1.159 ± 0.03
1.236AsnVal: 1.236 ± 0.024
0.297AsnTrp: 0.297 ± 0.011
0.369AsnTyr: 0.369 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
10.957ProAla: 10.957 ± 0.087
0.387ProCys: 0.387 ± 0.013
4.617ProAsp: 4.617 ± 0.049
4.236ProGlu: 4.236 ± 0.047
1.503ProPhe: 1.503 ± 0.028
7.297ProGly: 7.297 ± 0.072
1.47ProHis: 1.47 ± 0.026
1.362ProIle: 1.362 ± 0.025
1.054ProLys: 1.054 ± 0.025
5.505ProLeu: 5.505 ± 0.045
0.975ProMet: 0.975 ± 0.02
0.967ProAsn: 0.967 ± 0.026
4.148ProPro: 4.148 ± 0.073
1.992ProGln: 1.992 ± 0.036
4.096ProArg: 4.096 ± 0.05
3.29ProSer: 3.29 ± 0.05
3.659ProThr: 3.659 ± 0.048
5.812ProVal: 5.812 ± 0.06
0.943ProTrp: 0.943 ± 0.016
1.345ProTyr: 1.345 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
4.038GlnAla: 4.038 ± 0.051
0.169GlnCys: 0.169 ± 0.008
1.435GlnAsp: 1.435 ± 0.025
1.341GlnGlu: 1.341 ± 0.024
0.653GlnPhe: 0.653 ± 0.016
2.23GlnGly: 2.23 ± 0.035
0.717GlnHis: 0.717 ± 0.017
1.033GlnIle: 1.033 ± 0.023
0.55GlnLys: 0.55 ± 0.014
3.207GlnLeu: 3.207 ± 0.04
0.438GlnMet: 0.438 ± 0.013
0.517GlnAsn: 0.517 ± 0.017
1.928GlnPro: 1.928 ± 0.035
1.437GlnGln: 1.437 ± 0.037
2.345GlnArg: 2.345 ± 0.037
1.264GlnSer: 1.264 ± 0.024
1.397GlnThr: 1.397 ± 0.026
2.489GlnVal: 2.489 ± 0.027
0.507GlnTrp: 0.507 ± 0.016
0.617GlnTyr: 0.617 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
10.432ArgAla: 10.432 ± 0.083
0.65ArgCys: 0.65 ± 0.018
3.893ArgAsp: 3.893 ± 0.044
4.373ArgGlu: 4.373 ± 0.045
2.22ArgPhe: 2.22 ± 0.03
5.763ArgGly: 5.763 ± 0.053
2.179ArgHis: 2.179 ± 0.036
3.358ArgIle: 3.358 ± 0.035
1.352ArgLys: 1.352 ± 0.028
9.375ArgLeu: 9.375 ± 0.084
1.668ArgMet: 1.668 ± 0.028
1.308ArgAsn: 1.308 ± 0.025
5.968ArgPro: 5.968 ± 0.053
2.423ArgGln: 2.423 ± 0.032
8.45ArgArg: 8.45 ± 0.091
4.189ArgSer: 4.189 ± 0.044
5.724ArgThr: 5.724 ± 0.053
5.417ArgVal: 5.417 ± 0.046
1.474ArgTrp: 1.474 ± 0.027
1.832ArgTyr: 1.832 ± 0.028
0.0ArgXaa: 0.0 ± 0.0
Ser
6.864SerAla: 6.864 ± 0.059
0.444SerCys: 0.444 ± 0.015
2.432SerAsp: 2.432 ± 0.036
2.037SerGlu: 2.037 ± 0.036
1.4SerPhe: 1.4 ± 0.028
5.699SerGly: 5.699 ± 0.064
0.988SerHis: 0.988 ± 0.022
1.362SerIle: 1.362 ± 0.025
0.866SerLys: 0.866 ± 0.02
4.374SerLeu: 4.374 ± 0.046
0.938SerMet: 0.938 ± 0.02
0.852SerAsn: 0.852 ± 0.023
3.217SerPro: 3.217 ± 0.043
1.143SerGln: 1.143 ± 0.022
3.528SerArg: 3.528 ± 0.039
2.745SerSer: 2.745 ± 0.042
3.162SerThr: 3.162 ± 0.04
3.894SerVal: 3.894 ± 0.04
0.915SerTrp: 0.915 ± 0.02
1.13SerTyr: 1.13 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
10.307ThrAla: 10.307 ± 0.085
0.474ThrCys: 0.474 ± 0.015
3.641ThrAsp: 3.641 ± 0.046
3.061ThrGlu: 3.061 ± 0.035
1.503ThrPhe: 1.503 ± 0.025
7.069ThrGly: 7.069 ± 0.055
1.067ThrHis: 1.067 ± 0.022
1.511ThrIle: 1.511 ± 0.028
0.948ThrLys: 0.948 ± 0.025
5.543ThrLeu: 5.543 ± 0.05
0.867ThrMet: 0.867 ± 0.019
0.984ThrAsn: 0.984 ± 0.024
4.303ThrPro: 4.303 ± 0.047
1.223ThrGln: 1.223 ± 0.025
3.889ThrArg: 3.889 ± 0.042
2.918ThrSer: 2.918 ± 0.042
3.971ThrThr: 3.971 ± 0.058
6.487ThrVal: 6.487 ± 0.059
0.931ThrTrp: 0.931 ± 0.021
1.162ThrTyr: 1.162 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.718ValAla: 10.718 ± 0.082
0.723ValCys: 0.723 ± 0.017
4.929ValAsp: 4.929 ± 0.042
4.543ValGlu: 4.543 ± 0.052
2.245ValPhe: 2.245 ± 0.033
6.444ValGly: 6.444 ± 0.063
2.05ValHis: 2.05 ± 0.031
2.438ValIle: 2.438 ± 0.033
1.559ValLys: 1.559 ± 0.029
9.926ValLeu: 9.926 ± 0.094
1.246ValMet: 1.246 ± 0.021
1.587ValAsn: 1.587 ± 0.032
5.58ValPro: 5.58 ± 0.06
2.147ValGln: 2.147 ± 0.029
7.013ValArg: 7.013 ± 0.058
3.959ValSer: 3.959 ± 0.041
5.108ValThr: 5.108 ± 0.062
7.632ValVal: 7.632 ± 0.078
1.062ValTrp: 1.062 ± 0.02
1.464ValTyr: 1.464 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.787TrpAla: 1.787 ± 0.031
0.168TrpCys: 0.168 ± 0.008
0.803TrpAsp: 0.803 ± 0.021
0.714TrpGlu: 0.714 ± 0.017
0.498TrpPhe: 0.498 ± 0.015
1.02TrpGly: 1.02 ± 0.021
0.413TrpHis: 0.413 ± 0.013
0.525TrpIle: 0.525 ± 0.014
0.335TrpLys: 0.335 ± 0.014
1.814TrpLeu: 1.814 ± 0.029
0.265TrpMet: 0.265 ± 0.011
0.401TrpAsn: 0.401 ± 0.014
0.862TrpPro: 0.862 ± 0.02
0.694TrpGln: 0.694 ± 0.019
1.371TrpArg: 1.371 ± 0.024
0.94TrpSer: 0.94 ± 0.023
1.102TrpThr: 1.102 ± 0.021
0.98TrpVal: 0.98 ± 0.02
0.373TrpTrp: 0.373 ± 0.013
0.385TrpTyr: 0.385 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.458TyrAla: 2.458 ± 0.034
0.177TyrCys: 0.177 ± 0.009
1.31TyrAsp: 1.31 ± 0.032
0.997TyrGlu: 0.997 ± 0.02
0.588TyrPhe: 0.588 ± 0.017
2.156TyrGly: 2.156 ± 0.03
0.391TyrHis: 0.391 ± 0.012
0.422TyrIle: 0.422 ± 0.013
0.323TyrLys: 0.323 ± 0.013
2.194TyrLeu: 2.194 ± 0.032
0.206TyrMet: 0.206 ± 0.01
0.427TyrAsn: 0.427 ± 0.016
1.117TyrPro: 1.117 ± 0.026
0.628TyrGln: 0.628 ± 0.014
1.952TyrArg: 1.952 ± 0.028
0.932TyrSer: 0.932 ± 0.023
1.216TyrThr: 1.216 ± 0.027
1.392TyrVal: 1.392 ± 0.026
0.343TyrTrp: 0.343 ± 0.013
0.453TyrTyr: 0.453 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8063 proteins (2565824 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski