Shallot virus X (ShVX)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus; Acarallexivirus

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q04582|TGB2_SHVX Movement protein TGB2 OS=Shallot virus X OX=31770 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.15KK2 pKa = 9.91TDD4 pKa = 4.67LLLQILSNNNFTRR17 pKa = 11.84TSEE20 pKa = 4.42PIKK23 pKa = 10.5EE24 pKa = 3.92PLIIHH29 pKa = 6.47GVPGSGKK36 pKa = 8.19STLVRR41 pKa = 11.84ALVTYY46 pKa = 10.2RR47 pKa = 11.84STVACTLGAPYY58 pKa = 10.47GSNLAFPGVTSPGLTQSLTDD78 pKa = 3.41HH79 pKa = 6.21EE80 pKa = 4.5TRR82 pKa = 11.84ILDD85 pKa = 3.83EE86 pKa = 4.32YY87 pKa = 11.43QLGTEE92 pKa = 4.32SDD94 pKa = 3.68LKK96 pKa = 10.65PFNVLVGDD104 pKa = 4.27PFQGNLHH111 pKa = 6.56LKK113 pKa = 8.43AHH115 pKa = 5.15YY116 pKa = 9.97VKK118 pKa = 10.49SFSHH122 pKa = 6.12RR123 pKa = 11.84VPRR126 pKa = 11.84IICNFLQSLGYY137 pKa = 9.86EE138 pKa = 4.16IAGSKK143 pKa = 10.07PGEE146 pKa = 4.07LAQLPIYY153 pKa = 9.83GPNPSGPTGQVLHH166 pKa = 6.95LGPLSRR172 pKa = 11.84RR173 pKa = 11.84LTQSHH178 pKa = 6.1GVCSKK183 pKa = 10.85LPSEE187 pKa = 4.43VQGLEE192 pKa = 4.08FEE194 pKa = 4.58EE195 pKa = 4.56VTLVYY200 pKa = 10.42HH201 pKa = 6.47SSEE204 pKa = 4.1FEE206 pKa = 3.93RR207 pKa = 11.84NRR209 pKa = 11.84VGFYY213 pKa = 10.13IAATRR218 pKa = 11.84ALGRR222 pKa = 11.84LNLITDD228 pKa = 3.93TTLEE232 pKa = 4.46IPHH235 pKa = 6.34EE236 pKa = 4.34LCPTSS241 pKa = 3.27

Molecular weight: 26.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q04583|ORF4_SHVX Uncharacterized ORF4 protein OS=Shallot virus X OX=31770 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.4SFAPPPDD8 pKa = 3.29YY9 pKa = 11.38SKK11 pKa = 10.75IYY13 pKa = 9.97LALGCGLGLGFVVYY27 pKa = 10.61ASRR30 pKa = 11.84VNHH33 pKa = 6.42LPHH36 pKa = 8.21VGDD39 pKa = 3.63NTHH42 pKa = 6.49NLPHH46 pKa = 6.89GGQYY50 pKa = 10.57CDD52 pKa = 3.01GNKK55 pKa = 9.48RR56 pKa = 11.84VLYY59 pKa = 10.0SGPKK63 pKa = 9.61SGSSPTNNLWPFITVIALTLAILLTSCPRR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84VCIRR98 pKa = 11.84CSQHH102 pKa = 6.04HH103 pKa = 5.88

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2832

103

1718

472.0

52.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.309 ± 0.884

1.977 ± 0.616

5.191 ± 0.606

5.049 ± 0.594

3.919 ± 0.248

5.261 ± 0.825

3.319 ± 0.257

4.767 ± 0.273

5.544 ± 0.805

10.381 ± 1.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.907 ± 0.286

5.014 ± 0.826

6.321 ± 0.382

4.732 ± 0.451

5.756 ± 0.326

7.203 ± 0.97

7.556 ± 0.726

4.802 ± 0.438

0.847 ± 0.272

3.143 ± 0.439
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski