Tetragenococcus halophilus (strain DSM 20338 / JCM 20259 / NCIMB 9735 / NBRC 12172) (Pediococcus halophilus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Tetragenococcus; Tetragenococcus halophilus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4L6J7|G4L6J7_TETHN Uncharacterized protein OS=Tetragenococcus halophilus (strain DSM 20338 / JCM 20259 / NCIMB 9735 / NBRC 12172) OX=945021 GN=TEH_08440 PE=4 SV=1
MM1 pKa = 7.3TSAKK5 pKa = 9.67IIYY8 pKa = 10.1GSLTGNTEE16 pKa = 3.83VIADD20 pKa = 4.14IVCEE24 pKa = 3.81ALEE27 pKa = 4.04NLNIEE32 pKa = 4.16VDD34 pKa = 3.44IDD36 pKa = 3.43EE37 pKa = 4.78CTQIDD42 pKa = 3.66AEE44 pKa = 4.35EE45 pKa = 4.16FKK47 pKa = 11.01EE48 pKa = 4.45ADD50 pKa = 2.95ICIIAAYY57 pKa = 8.58TYY59 pKa = 10.95DD60 pKa = 3.49EE61 pKa = 4.28GTIPEE66 pKa = 4.37EE67 pKa = 3.86MLDD70 pKa = 3.74LYY72 pKa = 11.29EE73 pKa = 6.54DD74 pKa = 4.61LLQLDD79 pKa = 4.04LQGKK83 pKa = 8.37VFGVCGSGDD92 pKa = 3.44TFYY95 pKa = 11.31EE96 pKa = 4.37NYY98 pKa = 8.78ATAVDD103 pKa = 3.97SFEE106 pKa = 4.33EE107 pKa = 4.27AFLTAGAKK115 pKa = 10.12KK116 pKa = 8.61GTEE119 pKa = 4.04KK120 pKa = 10.64IKK122 pKa = 10.92VDD124 pKa = 4.16LAAEE128 pKa = 4.2EE129 pKa = 4.74EE130 pKa = 4.99DD131 pKa = 3.61IQQLKK136 pKa = 10.71AFASEE141 pKa = 4.76LVTTQQAMII150 pKa = 4.38

Molecular weight: 16.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4L543|G4L543_TETHN Uncharacterized protein OS=Tetragenococcus halophilus (strain DSM 20338 / JCM 20259 / NCIMB 9735 / NBRC 12172) OX=945021 GN=TEH_03400 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 9.14RR10 pKa = 11.84KK11 pKa = 8.54HH12 pKa = 5.23QKK14 pKa = 8.76VHH16 pKa = 5.58GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.21NGRR28 pKa = 11.84HH29 pKa = 5.26VIASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.71GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2507

0

2507

720916

33

2141

287.6

32.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.967 ± 0.047

0.61 ± 0.013

5.818 ± 0.046

7.498 ± 0.058

4.612 ± 0.04

6.333 ± 0.046

1.825 ± 0.021

7.541 ± 0.049

7.026 ± 0.047

9.639 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.02

4.859 ± 0.037

3.301 ± 0.025

4.599 ± 0.039

3.805 ± 0.034

5.974 ± 0.036

5.594 ± 0.03

6.662 ± 0.04

0.919 ± 0.019

3.744 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski