Amino acid dipepetide frequency for Neorhizobium sp. S3-V5DH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.37AlaAla: 15.37 ± 0.128
0.958AlaCys: 0.958 ± 0.026
6.409AlaAsp: 6.409 ± 0.064
7.462AlaGlu: 7.462 ± 0.074
4.532AlaPhe: 4.532 ± 0.059
9.981AlaGly: 9.981 ± 0.089
1.994AlaHis: 1.994 ± 0.036
6.765AlaIle: 6.765 ± 0.064
4.355AlaLys: 4.355 ± 0.057
11.959AlaLeu: 11.959 ± 0.089
3.604AlaMet: 3.604 ± 0.051
2.976AlaAsn: 2.976 ± 0.039
4.665AlaPro: 4.665 ± 0.069
3.354AlaGln: 3.354 ± 0.047
7.381AlaArg: 7.381 ± 0.075
6.419AlaSer: 6.419 ± 0.059
5.486AlaThr: 5.486 ± 0.06
8.477AlaVal: 8.477 ± 0.083
1.358AlaTrp: 1.358 ± 0.029
2.526AlaTyr: 2.526 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
0.825CysAla: 0.825 ± 0.025
0.097CysCys: 0.097 ± 0.008
0.488CysAsp: 0.488 ± 0.018
0.44CysGlu: 0.44 ± 0.017
0.336CysPhe: 0.336 ± 0.015
0.906CysGly: 0.906 ± 0.025
0.242CysHis: 0.242 ± 0.012
0.399CysIle: 0.399 ± 0.015
0.19CysLys: 0.19 ± 0.011
0.799CysLeu: 0.799 ± 0.023
0.17CysMet: 0.17 ± 0.01
0.191CysAsn: 0.191 ± 0.011
0.375CysPro: 0.375 ± 0.017
0.219CysGln: 0.219 ± 0.01
0.593CysArg: 0.593 ± 0.02
0.453CysSer: 0.453 ± 0.018
0.358CysThr: 0.358 ± 0.014
0.581CysVal: 0.581 ± 0.02
0.108CysTrp: 0.108 ± 0.008
0.209CysTyr: 0.209 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.081AspAla: 6.081 ± 0.062
0.469AspCys: 0.469 ± 0.017
2.966AspAsp: 2.966 ± 0.052
3.546AspGlu: 3.546 ± 0.048
2.343AspPhe: 2.343 ± 0.036
4.832AspGly: 4.832 ± 0.056
1.212AspHis: 1.212 ± 0.027
3.452AspIle: 3.452 ± 0.051
1.855AspLys: 1.855 ± 0.039
5.798AspLeu: 5.798 ± 0.061
1.419AspMet: 1.419 ± 0.026
1.429AspAsn: 1.429 ± 0.026
3.259AspPro: 3.259 ± 0.044
1.725AspGln: 1.725 ± 0.031
4.182AspArg: 4.182 ± 0.059
2.085AspSer: 2.085 ± 0.035
2.452AspThr: 2.452 ± 0.039
4.034AspVal: 4.034 ± 0.057
0.934AspTrp: 0.934 ± 0.026
1.533AspTyr: 1.533 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.487GluAla: 7.487 ± 0.074
0.365GluCys: 0.365 ± 0.014
3.038GluAsp: 3.038 ± 0.052
3.838GluGlu: 3.838 ± 0.054
2.006GluPhe: 2.006 ± 0.035
4.399GluGly: 4.399 ± 0.052
1.169GluHis: 1.169 ± 0.027
3.934GluIle: 3.934 ± 0.058
3.043GluLys: 3.043 ± 0.047
5.506GluLeu: 5.506 ± 0.06
1.658GluMet: 1.658 ± 0.031
1.973GluAsn: 1.973 ± 0.039
2.768GluPro: 2.768 ± 0.049
2.087GluGln: 2.087 ± 0.036
4.797GluArg: 4.797 ± 0.064
2.402GluSer: 2.402 ± 0.041
3.735GluThr: 3.735 ± 0.05
3.929GluVal: 3.929 ± 0.048
0.759GluTrp: 0.759 ± 0.021
1.059GluTyr: 1.059 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.584PheAla: 4.584 ± 0.06
0.414PheCys: 0.414 ± 0.016
2.696PheAsp: 2.696 ± 0.039
2.319PheGlu: 2.319 ± 0.034
1.657PhePhe: 1.657 ± 0.034
3.907PheGly: 3.907 ± 0.048
0.824PheHis: 0.824 ± 0.023
1.931PheIle: 1.931 ± 0.035
1.182PheLys: 1.182 ± 0.03
3.726PheLeu: 3.726 ± 0.058
0.896PheMet: 0.896 ± 0.024
1.171PheAsn: 1.171 ± 0.026
1.74PhePro: 1.74 ± 0.032
1.131PheGln: 1.131 ± 0.025
2.407PheArg: 2.407 ± 0.039
2.637PheSer: 2.637 ± 0.039
1.998PheThr: 1.998 ± 0.033
3.009PheVal: 3.009 ± 0.04
0.604PheTrp: 0.604 ± 0.019
0.989PheTyr: 0.989 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
8.148GlyAla: 8.148 ± 0.08
0.784GlyCys: 0.784 ± 0.021
4.215GlyAsp: 4.215 ± 0.072
4.89GlyGlu: 4.89 ± 0.05
3.771GlyPhe: 3.771 ± 0.047
6.91GlyGly: 6.91 ± 0.082
1.838GlyHis: 1.838 ± 0.033
5.142GlyIle: 5.142 ± 0.057
3.679GlyLys: 3.679 ± 0.045
8.569GlyLeu: 8.569 ± 0.087
2.339GlyMet: 2.339 ± 0.037
2.386GlyAsn: 2.386 ± 0.042
3.233GlyPro: 3.233 ± 0.051
2.732GlyGln: 2.732 ± 0.042
5.834GlyArg: 5.834 ± 0.055
4.825GlySer: 4.825 ± 0.057
4.433GlyThr: 4.433 ± 0.055
5.739GlyVal: 5.739 ± 0.058
1.298GlyTrp: 1.298 ± 0.026
2.445GlyTyr: 2.445 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.087HisAla: 2.087 ± 0.033
0.214HisCys: 0.214 ± 0.011
1.216HisAsp: 1.216 ± 0.032
1.092HisGlu: 1.092 ± 0.026
0.926HisPhe: 0.926 ± 0.024
1.845HisGly: 1.845 ± 0.031
0.6HisHis: 0.6 ± 0.023
1.028HisIle: 1.028 ± 0.026
0.544HisLys: 0.544 ± 0.018
2.071HisLeu: 2.071 ± 0.03
0.563HisMet: 0.563 ± 0.017
0.482HisAsn: 0.482 ± 0.017
1.31HisPro: 1.31 ± 0.026
0.627HisGln: 0.627 ± 0.02
1.433HisArg: 1.433 ± 0.028
1.01HisSer: 1.01 ± 0.025
0.816HisThr: 0.816 ± 0.022
1.496HisVal: 1.496 ± 0.031
0.3HisTrp: 0.3 ± 0.014
0.557HisTyr: 0.557 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.528IleAla: 7.528 ± 0.069
0.576IleCys: 0.576 ± 0.02
3.754IleAsp: 3.754 ± 0.047
3.908IleGlu: 3.908 ± 0.053
2.197IlePhe: 2.197 ± 0.039
5.369IleGly: 5.369 ± 0.067
1.035IleHis: 1.035 ± 0.025
2.798IleIle: 2.798 ± 0.042
1.69IleLys: 1.69 ± 0.033
5.091IleLeu: 5.091 ± 0.063
1.17IleMet: 1.17 ± 0.027
1.597IleAsn: 1.597 ± 0.03
2.497IlePro: 2.497 ± 0.042
1.366IleGln: 1.366 ± 0.033
3.69IleArg: 3.69 ± 0.048
3.58IleSer: 3.58 ± 0.037
2.902IleThr: 2.902 ± 0.047
4.459IleVal: 4.459 ± 0.052
0.679IleTrp: 0.679 ± 0.02
1.312IleTyr: 1.312 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.811LysAla: 4.811 ± 0.062
0.151LysCys: 0.151 ± 0.009
1.923LysAsp: 1.923 ± 0.039
2.063LysGlu: 2.063 ± 0.037
1.066LysPhe: 1.066 ± 0.027
2.932LysGly: 2.932 ± 0.04
0.646LysHis: 0.646 ± 0.018
2.188LysIle: 2.188 ± 0.04
1.628LysLys: 1.628 ± 0.038
3.826LysLeu: 3.826 ± 0.054
0.995LysMet: 0.995 ± 0.022
1.162LysAsn: 1.162 ± 0.03
2.296LysPro: 2.296 ± 0.042
1.147LysGln: 1.147 ± 0.026
2.607LysArg: 2.607 ± 0.037
2.21LysSer: 2.21 ± 0.038
2.239LysThr: 2.239 ± 0.037
2.749LysVal: 2.749 ± 0.043
0.424LysTrp: 0.424 ± 0.017
0.684LysTyr: 0.684 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
12.433LeuAla: 12.433 ± 0.095
0.877LeuCys: 0.877 ± 0.025
5.785LeuAsp: 5.785 ± 0.061
5.38LeuGlu: 5.38 ± 0.052
3.824LeuPhe: 3.824 ± 0.051
7.854LeuGly: 7.854 ± 0.082
1.832LeuHis: 1.832 ± 0.03
5.404LeuIle: 5.404 ± 0.065
4.259LeuLys: 4.259 ± 0.049
9.403LeuLeu: 9.403 ± 0.106
2.489LeuMet: 2.489 ± 0.038
2.772LeuAsn: 2.772 ± 0.047
5.439LeuPro: 5.439 ± 0.058
2.799LeuGln: 2.799 ± 0.05
6.16LeuArg: 6.16 ± 0.07
7.036LeuSer: 7.036 ± 0.063
5.49LeuThr: 5.49 ± 0.062
7.241LeuVal: 7.241 ± 0.071
1.136LeuTrp: 1.136 ± 0.029
2.152LeuTyr: 2.152 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
3.17MetAla: 3.17 ± 0.047
0.137MetCys: 0.137 ± 0.009
1.198MetAsp: 1.198 ± 0.026
1.31MetGlu: 1.31 ± 0.028
0.754MetPhe: 0.754 ± 0.021
1.836MetGly: 1.836 ± 0.034
0.44MetHis: 0.44 ± 0.014
1.615MetIle: 1.615 ± 0.032
1.223MetLys: 1.223 ± 0.03
2.695MetLeu: 2.695 ± 0.049
0.74MetMet: 0.74 ± 0.023
0.936MetAsn: 0.936 ± 0.023
1.557MetPro: 1.557 ± 0.034
0.9MetGln: 0.9 ± 0.025
1.828MetArg: 1.828 ± 0.028
1.746MetSer: 1.746 ± 0.031
2.0MetThr: 2.0 ± 0.034
1.751MetVal: 1.751 ± 0.03
0.213MetTrp: 0.213 ± 0.012
0.331MetTyr: 0.331 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.165AsnAla: 3.165 ± 0.045
0.265AsnCys: 0.265 ± 0.012
1.517AsnAsp: 1.517 ± 0.033
1.449AsnGlu: 1.449 ± 0.03
1.118AsnPhe: 1.118 ± 0.028
2.614AsnGly: 2.614 ± 0.044
0.574AsnHis: 0.574 ± 0.018
1.603AsnIle: 1.603 ± 0.033
0.81AsnLys: 0.81 ± 0.023
2.881AsnLeu: 2.881 ± 0.041
0.738AsnMet: 0.738 ± 0.02
0.798AsnAsn: 0.798 ± 0.023
1.995AsnPro: 1.995 ± 0.035
0.859AsnGln: 0.859 ± 0.025
2.059AsnArg: 2.059 ± 0.035
1.506AsnSer: 1.506 ± 0.033
1.323AsnThr: 1.323 ± 0.029
2.023AsnVal: 2.023 ± 0.031
0.474AsnTrp: 0.474 ± 0.016
0.745AsnTyr: 0.745 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
5.625ProAla: 5.625 ± 0.074
0.285ProCys: 0.285 ± 0.012
3.394ProAsp: 3.394 ± 0.049
3.734ProGlu: 3.734 ± 0.045
2.06ProPhe: 2.06 ± 0.035
4.023ProGly: 4.023 ± 0.054
1.087ProHis: 1.087 ± 0.025
2.447ProIle: 2.447 ± 0.045
1.91ProLys: 1.91 ± 0.033
4.654ProLeu: 4.654 ± 0.059
1.224ProMet: 1.224 ± 0.025
1.475ProAsn: 1.475 ± 0.031
2.312ProPro: 2.312 ± 0.045
1.694ProGln: 1.694 ± 0.035
2.604ProArg: 2.604 ± 0.043
2.874ProSer: 2.874 ± 0.044
2.345ProThr: 2.345 ± 0.038
4.124ProVal: 4.124 ± 0.057
0.624ProTrp: 0.624 ± 0.018
1.229ProTyr: 1.229 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.781GlnAla: 3.781 ± 0.055
0.173GlnCys: 0.173 ± 0.01
1.378GlnAsp: 1.378 ± 0.024
1.669GlnGlu: 1.669 ± 0.034
1.102GlnPhe: 1.102 ± 0.026
2.271GlnGly: 2.271 ± 0.045
0.587GlnHis: 0.587 ± 0.019
1.944GlnIle: 1.944 ± 0.034
1.293GlnLys: 1.293 ± 0.029
2.8GlnLeu: 2.8 ± 0.041
0.974GlnMet: 0.974 ± 0.024
0.952GlnAsn: 0.952 ± 0.021
1.761GlnPro: 1.761 ± 0.036
1.31GlnGln: 1.31 ± 0.038
2.195GlnArg: 2.195 ± 0.035
1.867GlnSer: 1.867 ± 0.038
1.751GlnThr: 1.751 ± 0.035
2.137GlnVal: 2.137 ± 0.038
0.372GlnTrp: 0.372 ± 0.015
0.645GlnTyr: 0.645 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
6.598ArgAla: 6.598 ± 0.07
0.447ArgCys: 0.447 ± 0.016
3.817ArgAsp: 3.817 ± 0.056
4.197ArgGlu: 4.197 ± 0.055
2.931ArgPhe: 2.931 ± 0.043
4.402ArgGly: 4.402 ± 0.052
1.712ArgHis: 1.712 ± 0.03
4.181ArgIle: 4.181 ± 0.05
2.603ArgLys: 2.603 ± 0.045
7.326ArgLeu: 7.326 ± 0.069
1.923ArgMet: 1.923 ± 0.037
2.053ArgAsn: 2.053 ± 0.034
3.271ArgPro: 3.271 ± 0.053
2.649ArgGln: 2.649 ± 0.041
5.249ArgArg: 5.249 ± 0.066
3.893ArgSer: 3.893 ± 0.05
3.191ArgThr: 3.191 ± 0.049
4.273ArgVal: 4.273 ± 0.051
0.916ArgTrp: 0.916 ± 0.026
1.782ArgTyr: 1.782 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.215SerAla: 6.215 ± 0.062
0.411SerCys: 0.411 ± 0.016
3.153SerAsp: 3.153 ± 0.049
3.302SerGlu: 3.302 ± 0.052
2.55SerPhe: 2.55 ± 0.041
5.707SerGly: 5.707 ± 0.066
1.22SerHis: 1.22 ± 0.028
3.148SerIle: 3.148 ± 0.045
1.952SerLys: 1.952 ± 0.035
5.852SerLeu: 5.852 ± 0.056
1.441SerMet: 1.441 ± 0.028
1.564SerAsn: 1.564 ± 0.032
2.847SerPro: 2.847 ± 0.049
1.695SerGln: 1.695 ± 0.032
3.942SerArg: 3.942 ± 0.054
3.49SerSer: 3.49 ± 0.051
2.863SerThr: 2.863 ± 0.035
4.288SerVal: 4.288 ± 0.048
0.762SerTrp: 0.762 ± 0.018
1.426SerTyr: 1.426 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
6.002ThrAla: 6.002 ± 0.059
0.413ThrCys: 0.413 ± 0.016
2.689ThrAsp: 2.689 ± 0.041
2.843ThrGlu: 2.843 ± 0.04
2.11ThrPhe: 2.11 ± 0.035
4.941ThrGly: 4.941 ± 0.05
1.007ThrHis: 1.007 ± 0.023
3.151ThrIle: 3.151 ± 0.045
1.702ThrLys: 1.702 ± 0.032
5.527ThrLeu: 5.527 ± 0.062
1.253ThrMet: 1.253 ± 0.025
1.394ThrAsn: 1.394 ± 0.033
2.904ThrPro: 2.904 ± 0.043
1.361ThrGln: 1.361 ± 0.029
3.11ThrArg: 3.11 ± 0.043
2.998ThrSer: 2.998 ± 0.044
2.7ThrThr: 2.7 ± 0.04
4.325ThrVal: 4.325 ± 0.05
0.654ThrTrp: 0.654 ± 0.018
1.213ThrTyr: 1.213 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
8.571ValAla: 8.571 ± 0.075
0.59ValCys: 0.59 ± 0.019
3.889ValAsp: 3.889 ± 0.043
4.559ValGlu: 4.559 ± 0.05
2.918ValPhe: 2.918 ± 0.045
5.449ValGly: 5.449 ± 0.063
1.354ValHis: 1.354 ± 0.03
4.301ValIle: 4.301 ± 0.054
2.6ValLys: 2.6 ± 0.04
7.278ValLeu: 7.278 ± 0.074
1.927ValMet: 1.927 ± 0.036
2.073ValAsn: 2.073 ± 0.04
3.605ValPro: 3.605 ± 0.05
1.992ValGln: 1.992 ± 0.037
4.518ValArg: 4.518 ± 0.047
4.624ValSer: 4.624 ± 0.049
4.423ValThr: 4.423 ± 0.05
5.806ValVal: 5.806 ± 0.069
0.896ValTrp: 0.896 ± 0.024
1.51ValTyr: 1.51 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.154TrpAla: 1.154 ± 0.031
0.121TrpCys: 0.121 ± 0.008
0.616TrpAsp: 0.616 ± 0.02
0.589TrpGlu: 0.589 ± 0.019
0.569TrpPhe: 0.569 ± 0.018
0.881TrpGly: 0.881 ± 0.022
0.321TrpHis: 0.321 ± 0.013
0.706TrpIle: 0.706 ± 0.024
0.54TrpLys: 0.54 ± 0.018
1.635TrpLeu: 1.635 ± 0.033
0.373TrpMet: 0.373 ± 0.014
0.482TrpAsn: 0.482 ± 0.016
0.678TrpPro: 0.678 ± 0.022
0.571TrpGln: 0.571 ± 0.019
1.051TrpArg: 1.051 ± 0.028
0.841TrpSer: 0.841 ± 0.02
0.722TrpThr: 0.722 ± 0.017
0.736TrpVal: 0.736 ± 0.021
0.225TrpTrp: 0.225 ± 0.011
0.318TrpTyr: 0.318 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.397TyrAla: 2.397 ± 0.042
0.233TyrCys: 0.233 ± 0.012
1.465TyrAsp: 1.465 ± 0.029
1.361TyrGlu: 1.361 ± 0.03
1.051TyrPhe: 1.051 ± 0.026
2.149TyrGly: 2.149 ± 0.035
0.524TyrHis: 0.524 ± 0.02
1.063TyrIle: 1.063 ± 0.027
0.686TyrLys: 0.686 ± 0.019
2.37TyrLeu: 2.37 ± 0.042
0.486TyrMet: 0.486 ± 0.016
0.649TyrAsn: 0.649 ± 0.02
1.211TyrPro: 1.211 ± 0.03
0.745TyrGln: 0.745 ± 0.02
1.774TyrArg: 1.774 ± 0.031
1.332TyrSer: 1.332 ± 0.025
1.072TyrThr: 1.072 ± 0.026
1.706TyrVal: 1.706 ± 0.035
0.393TyrTrp: 0.393 ± 0.016
0.669TyrTyr: 0.669 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5607 proteins (1719071 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski