Steinernema carpocapsae (Entomopathogenic nematode)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Tylenchina; Panagrolaimomorpha; Strongyloidoidea; Steinernematidae; Steinernema

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U5P0T8|A0A4U5P0T8_STECR Uncharacterized protein OS=Steinernema carpocapsae OX=34508 GN=L596_013370 PE=4 SV=1
MM1 pKa = 7.47LADD4 pKa = 5.23LFDD7 pKa = 3.96TKK9 pKa = 11.35DD10 pKa = 3.57PLEE13 pKa = 4.29EE14 pKa = 5.5SMDD17 pKa = 4.14CDD19 pKa = 3.85GLDD22 pKa = 3.34VSASEE27 pKa = 4.12QSCAVNMAMEE37 pKa = 4.77DD38 pKa = 3.91QPSSSADD45 pKa = 3.3RR46 pKa = 11.84FEE48 pKa = 4.29VDD50 pKa = 3.51QVSSDD55 pKa = 3.52IEE57 pKa = 4.31DD58 pKa = 3.57MNLEE62 pKa = 4.52DD63 pKa = 6.07DD64 pKa = 4.56GHH66 pKa = 5.95TKK68 pKa = 10.74SNNQDD73 pKa = 3.53VIHH76 pKa = 6.87APINDD81 pKa = 3.38TRR83 pKa = 11.84MDD85 pKa = 3.79RR86 pKa = 11.84PDD88 pKa = 3.44IATLALTEE96 pKa = 4.29TTGLLIYY103 pKa = 9.63TEE105 pKa = 4.36NGCLCHH111 pKa = 6.76EE112 pKa = 5.2CDD114 pKa = 3.37PLYY117 pKa = 10.83YY118 pKa = 10.13SEE120 pKa = 5.08SNPMDD125 pKa = 3.86ISRR128 pKa = 11.84MSLL131 pKa = 3.05

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U8UP16|A0A4U8UP16_STECR UBX domain-containing protein OS=Steinernema carpocapsae OX=34508 GN=L596_002251 PE=4 SV=1
MM1 pKa = 7.53LHH3 pKa = 6.45GNKK6 pKa = 9.91ANSSKK11 pKa = 9.94TSTGTLPVLSLSPSPTEE28 pKa = 3.98SPIFGPRR35 pKa = 11.84HH36 pKa = 4.67TLRR39 pKa = 11.84IGVLLLLTSKK49 pKa = 10.19NWTRR53 pKa = 11.84TRR55 pKa = 11.84SIAKK59 pKa = 9.84KK60 pKa = 9.79KK61 pKa = 10.83ANLILKK67 pKa = 8.64MRR69 pKa = 11.84MVLRR73 pKa = 11.84RR74 pKa = 11.84QRR76 pKa = 11.84QRR78 pKa = 11.84TRR80 pKa = 11.84TRR82 pKa = 11.84KK83 pKa = 9.37KK84 pKa = 9.75QLSMWRR90 pKa = 11.84RR91 pKa = 3.31

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30620

5724

36344

11325030

66

10066

311.6

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.996 ± 0.013

2.182 ± 0.014

5.171 ± 0.015

6.495 ± 0.028

4.594 ± 0.012

5.513 ± 0.019

2.406 ± 0.009

5.178 ± 0.012

6.006 ± 0.021

8.913 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.007

4.379 ± 0.01

5.316 ± 0.02

3.971 ± 0.013

6.111 ± 0.017

8.168 ± 0.023

5.663 ± 0.025

6.469 ± 0.012

1.128 ± 0.005

2.859 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski