Pseudomonas phage AUS531phi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2FAD9|A0A5Q2FAD9_9CAUD Uncharacterized protein OS=Pseudomonas phage AUS531phi OX=2663264 PE=4 SV=1
MM1 pKa = 7.3TLITDD6 pKa = 3.82IQKK9 pKa = 10.58LEE11 pKa = 4.3PGGEE15 pKa = 4.11VVLFEE20 pKa = 6.1LDD22 pKa = 3.53GSDD25 pKa = 4.06FGADD29 pKa = 2.96VVRR32 pKa = 11.84FHH34 pKa = 6.39GHH36 pKa = 7.83AIPHH40 pKa = 5.97SPQEE44 pKa = 3.91LAAAGANADD53 pKa = 3.68QLPAKK58 pKa = 9.69SIWWQGHH65 pKa = 5.6EE66 pKa = 3.99YY67 pKa = 10.43AAWPVQIEE75 pKa = 4.58GIEE78 pKa = 4.1ANSDD82 pKa = 3.13GTAARR87 pKa = 11.84PSFTAGNVNGRR98 pKa = 11.84ITALCLAFEE107 pKa = 5.15DD108 pKa = 4.34LLQFRR113 pKa = 11.84LTIRR117 pKa = 11.84TTLARR122 pKa = 11.84YY123 pKa = 9.67LDD125 pKa = 3.96AANFPGGNPDD135 pKa = 3.96ADD137 pKa = 3.67PSQEE141 pKa = 4.01IVEE144 pKa = 4.07IWYY147 pKa = 10.12LDD149 pKa = 3.34QKK151 pKa = 9.85TNEE154 pKa = 4.33DD155 pKa = 3.29GQYY158 pKa = 10.26VAWEE162 pKa = 4.07LASPGDD168 pKa = 3.5VGGEE172 pKa = 3.79QVGRR176 pKa = 11.84QMTTLCHH183 pKa = 5.81WAMTGGYY190 pKa = 9.27RR191 pKa = 11.84GPDD194 pKa = 3.14CGYY197 pKa = 8.17TGPYY201 pKa = 9.62FDD203 pKa = 5.51IDD205 pKa = 3.98GNPTDD210 pKa = 4.85DD211 pKa = 4.39PARR214 pKa = 11.84DD215 pKa = 3.66EE216 pKa = 5.16CDD218 pKa = 3.03GCLGTGCIPRR228 pKa = 11.84FGEE231 pKa = 4.5GNQLPFGGFPAVSIIARR248 pKa = 11.84SS249 pKa = 3.25

Molecular weight: 26.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F486|A0A5Q2F486_9CAUD Uncharacterized protein OS=Pseudomonas phage AUS531phi OX=2663264 PE=4 SV=1
MM1 pKa = 6.37TTAAHH6 pKa = 5.91HH7 pKa = 6.35TPMTTIKK14 pKa = 10.57LYY16 pKa = 10.53GALRR20 pKa = 11.84QFGRR24 pKa = 11.84EE25 pKa = 3.6YY26 pKa = 11.21RR27 pKa = 11.84MLVGSTAEE35 pKa = 4.46AIKK38 pKa = 10.28ALCVQIPGLEE48 pKa = 3.72RR49 pKa = 11.84FLANAHH55 pKa = 5.46LRR57 pKa = 11.84GMEE60 pKa = 3.83FAVFRR65 pKa = 11.84GKK67 pKa = 10.75RR68 pKa = 11.84NISQDD73 pKa = 3.04EE74 pKa = 4.28LQFGGAEE81 pKa = 4.52EE82 pKa = 4.17IRR84 pKa = 11.84IAPVMRR90 pKa = 11.84GRR92 pKa = 11.84KK93 pKa = 9.3RR94 pKa = 11.84GGLVQTIVGVALIVAATIMAGPGGFAAAGGLTGAMGTAGVAMAIGGVIQMLSPQAQGLKK153 pKa = 10.16QSAAPEE159 pKa = 4.12NLPSYY164 pKa = 11.06AFGSARR170 pKa = 11.84NTTASGNPVPICYY183 pKa = 10.0GKK185 pKa = 10.33RR186 pKa = 11.84RR187 pKa = 11.84WGGAIISASIYY198 pKa = 11.15AEE200 pKa = 4.27DD201 pKa = 3.62KK202 pKa = 10.06TT203 pKa = 4.4

Molecular weight: 21.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13849

45

1063

209.8

22.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.362 ± 0.567

0.903 ± 0.113

6.058 ± 0.216

6.318 ± 0.308

2.881 ± 0.229

8.087 ± 0.282

2.108 ± 0.236

4.73 ± 0.214

4.065 ± 0.238

9.365 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.168

2.975 ± 0.202

4.773 ± 0.337

5.04 ± 0.251

7.199 ± 0.323

5.199 ± 0.251

5.112 ± 0.204

6.643 ± 0.312

1.574 ± 0.155

2.34 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski