Amino acid dipepetide frequency for Bacillus phage Bobb

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.009AlaAla: 6.009 ± 0.578
0.444AlaCys: 0.444 ± 0.123
4.042AlaAsp: 4.042 ± 0.362
5.65AlaGlu: 5.65 ± 0.444
2.412AlaPhe: 2.412 ± 0.226
4.063AlaGly: 4.063 ± 0.385
1.079AlaHis: 1.079 ± 0.138
4.02AlaIle: 4.02 ± 0.308
4.084AlaLys: 4.084 ± 0.281
5.396AlaLeu: 5.396 ± 0.38
1.629AlaMet: 1.629 ± 0.183
3.089AlaAsn: 3.089 ± 0.234
2.074AlaPro: 2.074 ± 0.437
2.814AlaGln: 2.814 ± 0.307
2.687AlaArg: 2.687 ± 0.227
3.301AlaSer: 3.301 ± 0.274
3.766AlaThr: 3.766 ± 0.384
5.163AlaVal: 5.163 ± 0.437
0.698AlaTrp: 0.698 ± 0.149
2.476AlaTyr: 2.476 ± 0.259
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.088
0.085CysCys: 0.085 ± 0.044
0.508CysAsp: 0.508 ± 0.103
0.487CysGlu: 0.487 ± 0.099
0.381CysPhe: 0.381 ± 0.084
0.339CysGly: 0.339 ± 0.081
0.254CysHis: 0.254 ± 0.076
0.296CysIle: 0.296 ± 0.079
0.762CysLys: 0.762 ± 0.14
0.614CysLeu: 0.614 ± 0.125
0.254CysMet: 0.254 ± 0.075
0.212CysAsn: 0.212 ± 0.067
0.423CysPro: 0.423 ± 0.096
0.085CysGln: 0.085 ± 0.041
0.36CysArg: 0.36 ± 0.12
0.55CysSer: 0.55 ± 0.114
0.423CysThr: 0.423 ± 0.114
0.529CysVal: 0.529 ± 0.113
0.169CysTrp: 0.169 ± 0.061
0.466CysTyr: 0.466 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
3.83AspAla: 3.83 ± 0.308
0.635AspCys: 0.635 ± 0.14
3.618AspAsp: 3.618 ± 0.327
4.824AspGlu: 4.824 ± 0.347
2.624AspPhe: 2.624 ± 0.234
3.682AspGly: 3.682 ± 0.383
1.206AspHis: 1.206 ± 0.159
4.486AspIle: 4.486 ± 0.289
4.698AspLys: 4.698 ± 0.338
5.269AspLeu: 5.269 ± 0.379
2.116AspMet: 2.116 ± 0.221
3.301AspAsn: 3.301 ± 0.242
1.883AspPro: 1.883 ± 0.214
2.412AspGln: 2.412 ± 0.193
3.005AspArg: 3.005 ± 0.297
3.618AspSer: 3.618 ± 0.288
3.724AspThr: 3.724 ± 0.304
4.803AspVal: 4.803 ± 0.309
0.656AspTrp: 0.656 ± 0.127
3.449AspTyr: 3.449 ± 0.258
0.0AspXaa: 0.0 ± 0.0
Glu
5.269GluAla: 5.269 ± 0.356
0.508GluCys: 0.508 ± 0.112
5.713GluAsp: 5.713 ± 0.461
9.818GluGlu: 9.818 ± 0.844
3.111GluPhe: 3.111 ± 0.274
5.375GluGly: 5.375 ± 0.389
1.714GluHis: 1.714 ± 0.23
4.782GluIle: 4.782 ± 0.403
6.285GluLys: 6.285 ± 0.481
8.02GluLeu: 8.02 ± 0.486
2.349GluMet: 2.349 ± 0.262
4.19GluAsn: 4.19 ± 0.301
1.841GluPro: 1.841 ± 0.213
2.984GluGln: 2.984 ± 0.27
3.915GluArg: 3.915 ± 0.308
4.126GluSer: 4.126 ± 0.304
3.428GluThr: 3.428 ± 0.28
5.946GluVal: 5.946 ± 0.387
0.973GluTrp: 0.973 ± 0.173
3.322GluTyr: 3.322 ± 0.277
0.0GluXaa: 0.0 ± 0.0
Phe
2.053PheAla: 2.053 ± 0.211
0.275PheCys: 0.275 ± 0.066
2.984PheAsp: 2.984 ± 0.252
3.068PheGlu: 3.068 ± 0.281
1.524PhePhe: 1.524 ± 0.197
2.053PheGly: 2.053 ± 0.234
1.016PheHis: 1.016 ± 0.145
3.216PheIle: 3.216 ± 0.275
3.026PheLys: 3.026 ± 0.233
3.047PheLeu: 3.047 ± 0.262
0.973PheMet: 0.973 ± 0.136
2.624PheAsn: 2.624 ± 0.227
1.185PhePro: 1.185 ± 0.156
1.185PheGln: 1.185 ± 0.183
1.672PheArg: 1.672 ± 0.206
3.132PheSer: 3.132 ± 0.28
2.433PheThr: 2.433 ± 0.235
2.518PheVal: 2.518 ± 0.241
0.296PheTrp: 0.296 ± 0.076
1.714PheTyr: 1.714 ± 0.222
0.0PheXaa: 0.0 ± 0.0
Gly
4.105GlyAla: 4.105 ± 0.494
0.402GlyCys: 0.402 ± 0.095
3.364GlyAsp: 3.364 ± 0.272
4.295GlyGlu: 4.295 ± 0.289
2.645GlyPhe: 2.645 ± 0.23
4.782GlyGly: 4.782 ± 0.719
1.46GlyHis: 1.46 ± 0.189
3.491GlyIle: 3.491 ± 0.369
4.824GlyLys: 4.824 ± 0.37
4.698GlyLeu: 4.698 ± 0.345
1.756GlyMet: 1.756 ± 0.2
3.343GlyAsn: 3.343 ± 0.233
0.317GlyPro: 0.317 ± 0.076
1.883GlyGln: 1.883 ± 0.235
2.497GlyArg: 2.497 ± 0.213
3.597GlySer: 3.597 ± 0.385
3.872GlyThr: 3.872 ± 0.336
4.592GlyVal: 4.592 ± 0.319
0.804GlyTrp: 0.804 ± 0.152
2.772GlyTyr: 2.772 ± 0.262
0.0GlyXaa: 0.0 ± 0.0
His
1.121HisAla: 1.121 ± 0.155
0.19HisCys: 0.19 ± 0.062
1.27HisAsp: 1.27 ± 0.19
1.439HisGlu: 1.439 ± 0.175
1.1HisPhe: 1.1 ± 0.144
1.079HisGly: 1.079 ± 0.161
0.656HisHis: 0.656 ± 0.127
1.672HisIle: 1.672 ± 0.204
1.375HisLys: 1.375 ± 0.178
1.841HisLeu: 1.841 ± 0.211
0.317HisMet: 0.317 ± 0.081
0.952HisAsn: 0.952 ± 0.139
0.635HisPro: 0.635 ± 0.104
0.444HisGln: 0.444 ± 0.093
0.973HisArg: 0.973 ± 0.142
1.439HisSer: 1.439 ± 0.164
1.121HisThr: 1.121 ± 0.154
1.46HisVal: 1.46 ± 0.188
0.36HisTrp: 0.36 ± 0.094
1.079HisTyr: 1.079 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
4.338IleAla: 4.338 ± 0.271
0.592IleCys: 0.592 ± 0.133
4.888IleAsp: 4.888 ± 0.332
5.142IleGlu: 5.142 ± 0.34
2.053IlePhe: 2.053 ± 0.215
3.491IleGly: 3.491 ± 0.3
1.143IleHis: 1.143 ± 0.153
3.534IleIle: 3.534 ± 0.301
4.613IleLys: 4.613 ± 0.299
4.549IleLeu: 4.549 ± 0.359
1.397IleMet: 1.397 ± 0.152
3.259IleAsn: 3.259 ± 0.301
2.116IlePro: 2.116 ± 0.205
2.264IleGln: 2.264 ± 0.191
2.962IleArg: 2.962 ± 0.225
4.147IleSer: 4.147 ± 0.326
4.169IleThr: 4.169 ± 0.358
4.232IleVal: 4.232 ± 0.299
0.339IleTrp: 0.339 ± 0.078
2.285IleTyr: 2.285 ± 0.192
0.0IleXaa: 0.0 ± 0.0
Lys
4.867LysAla: 4.867 ± 0.359
0.423LysCys: 0.423 ± 0.12
5.227LysAsp: 5.227 ± 0.33
7.406LysGlu: 7.406 ± 0.581
2.857LysPhe: 2.857 ± 0.271
4.401LysGly: 4.401 ± 0.323
1.524LysHis: 1.524 ± 0.197
4.338LysIle: 4.338 ± 0.337
6.475LysLys: 6.475 ± 0.508
5.607LysLeu: 5.607 ± 0.346
2.137LysMet: 2.137 ± 0.215
3.64LysAsn: 3.64 ± 0.222
2.137LysPro: 2.137 ± 0.22
3.343LysGln: 3.343 ± 0.272
3.872LysArg: 3.872 ± 0.27
4.338LysSer: 4.338 ± 0.365
3.513LysThr: 3.513 ± 0.325
4.867LysVal: 4.867 ± 0.333
0.846LysTrp: 0.846 ± 0.125
3.111LysTyr: 3.111 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
5.417LeuAla: 5.417 ± 0.305
0.55LeuCys: 0.55 ± 0.106
5.121LeuAsp: 5.121 ± 0.336
6.919LeuGlu: 6.919 ± 0.495
3.132LeuPhe: 3.132 ± 0.26
4.698LeuGly: 4.698 ± 0.369
1.841LeuHis: 1.841 ± 0.224
4.507LeuIle: 4.507 ± 0.332
6.475LeuLys: 6.475 ± 0.34
6.454LeuLeu: 6.454 ± 0.413
1.968LeuMet: 1.968 ± 0.189
4.592LeuAsn: 4.592 ± 0.32
3.089LeuPro: 3.089 ± 0.252
3.237LeuGln: 3.237 ± 0.303
4.19LeuArg: 4.19 ± 0.296
5.946LeuSer: 5.946 ± 0.381
5.988LeuThr: 5.988 ± 0.439
5.036LeuVal: 5.036 ± 0.324
0.825LeuTrp: 0.825 ± 0.124
3.153LeuTyr: 3.153 ± 0.265
0.0LeuXaa: 0.0 ± 0.0
Met
1.439MetAla: 1.439 ± 0.177
0.148MetCys: 0.148 ± 0.052
1.354MetAsp: 1.354 ± 0.176
2.116MetGlu: 2.116 ± 0.219
1.016MetPhe: 1.016 ± 0.168
1.1MetGly: 1.1 ± 0.164
0.36MetHis: 0.36 ± 0.082
1.799MetIle: 1.799 ± 0.194
2.243MetLys: 2.243 ± 0.263
2.053MetLeu: 2.053 ± 0.236
0.656MetMet: 0.656 ± 0.123
1.397MetAsn: 1.397 ± 0.169
0.91MetPro: 0.91 ± 0.155
1.058MetGln: 1.058 ± 0.164
1.312MetArg: 1.312 ± 0.178
1.947MetSer: 1.947 ± 0.177
1.989MetThr: 1.989 ± 0.178
1.248MetVal: 1.248 ± 0.186
0.296MetTrp: 0.296 ± 0.096
1.1MetTyr: 1.1 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
2.793AsnAla: 2.793 ± 0.249
0.423AsnCys: 0.423 ± 0.099
3.047AsnAsp: 3.047 ± 0.257
3.788AsnGlu: 3.788 ± 0.296
2.031AsnPhe: 2.031 ± 0.218
3.555AsnGly: 3.555 ± 0.34
1.058AsnHis: 1.058 ± 0.149
3.682AsnIle: 3.682 ± 0.303
3.597AsnLys: 3.597 ± 0.25
4.38AsnLeu: 4.38 ± 0.32
1.143AsnMet: 1.143 ± 0.177
2.878AsnAsn: 2.878 ± 0.296
2.645AsnPro: 2.645 ± 0.268
1.756AsnGln: 1.756 ± 0.233
2.751AsnArg: 2.751 ± 0.241
3.64AsnSer: 3.64 ± 0.324
3.068AsnThr: 3.068 ± 0.245
3.111AsnVal: 3.111 ± 0.238
0.466AsnTrp: 0.466 ± 0.103
2.243AsnTyr: 2.243 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
2.772ProAla: 2.772 ± 0.49
0.148ProCys: 0.148 ± 0.054
1.735ProAsp: 1.735 ± 0.22
2.433ProGlu: 2.433 ± 0.207
1.524ProPhe: 1.524 ± 0.196
0.825ProGly: 0.825 ± 0.132
0.762ProHis: 0.762 ± 0.141
1.883ProIle: 1.883 ± 0.177
1.777ProLys: 1.777 ± 0.187
2.73ProLeu: 2.73 ± 0.238
0.635ProMet: 0.635 ± 0.122
1.735ProAsn: 1.735 ± 0.208
1.164ProPro: 1.164 ± 0.261
1.354ProGln: 1.354 ± 0.195
1.248ProArg: 1.248 ± 0.154
2.391ProSer: 2.391 ± 0.272
2.306ProThr: 2.306 ± 0.239
1.629ProVal: 1.629 ± 0.202
0.106ProTrp: 0.106 ± 0.043
1.756ProTyr: 1.756 ± 0.211
0.0ProXaa: 0.0 ± 0.0
Gln
2.518GlnAla: 2.518 ± 0.256
0.212GlnCys: 0.212 ± 0.077
2.179GlnAsp: 2.179 ± 0.258
3.513GlnGlu: 3.513 ± 0.315
1.397GlnPhe: 1.397 ± 0.165
2.01GlnGly: 2.01 ± 0.218
0.635GlnHis: 0.635 ± 0.106
1.904GlnIle: 1.904 ± 0.178
2.666GlnLys: 2.666 ± 0.271
3.893GlnLeu: 3.893 ± 0.265
1.058GlnMet: 1.058 ± 0.152
1.545GlnAsn: 1.545 ± 0.186
1.1GlnPro: 1.1 ± 0.202
1.989GlnGln: 1.989 ± 0.221
1.375GlnArg: 1.375 ± 0.16
2.433GlnSer: 2.433 ± 0.241
2.074GlnThr: 2.074 ± 0.261
2.624GlnVal: 2.624 ± 0.281
0.402GlnTrp: 0.402 ± 0.099
1.65GlnTyr: 1.65 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
2.73ArgAla: 2.73 ± 0.257
0.466ArgCys: 0.466 ± 0.122
2.984ArgAsp: 2.984 ± 0.261
3.957ArgGlu: 3.957 ± 0.331
1.947ArgPhe: 1.947 ± 0.196
2.772ArgGly: 2.772 ± 0.27
0.889ArgHis: 0.889 ± 0.128
3.089ArgIle: 3.089 ± 0.27
4.19ArgLys: 4.19 ± 0.313
4.105ArgLeu: 4.105 ± 0.319
1.439ArgMet: 1.439 ± 0.186
2.222ArgAsn: 2.222 ± 0.204
1.121ArgPro: 1.121 ± 0.142
1.777ArgGln: 1.777 ± 0.19
2.455ArgArg: 2.455 ± 0.295
2.603ArgSer: 2.603 ± 0.259
2.708ArgThr: 2.708 ± 0.224
3.428ArgVal: 3.428 ± 0.237
0.402ArgTrp: 0.402 ± 0.096
1.968ArgTyr: 1.968 ± 0.177
0.0ArgXaa: 0.0 ± 0.0
Ser
4.169SerAla: 4.169 ± 0.399
0.466SerCys: 0.466 ± 0.102
3.978SerAsp: 3.978 ± 0.332
4.105SerGlu: 4.105 ± 0.281
3.237SerPhe: 3.237 ± 0.269
3.999SerGly: 3.999 ± 0.366
1.143SerHis: 1.143 ± 0.144
4.042SerIle: 4.042 ± 0.338
4.951SerLys: 4.951 ± 0.313
5.396SerLeu: 5.396 ± 0.298
1.926SerMet: 1.926 ± 0.219
3.407SerAsn: 3.407 ± 0.335
2.095SerPro: 2.095 ± 0.247
2.222SerGln: 2.222 ± 0.224
3.386SerArg: 3.386 ± 0.284
4.973SerSer: 4.973 ± 0.445
4.126SerThr: 4.126 ± 0.455
3.872SerVal: 3.872 ± 0.306
0.719SerTrp: 0.719 ± 0.122
2.518SerTyr: 2.518 ± 0.188
0.0SerXaa: 0.0 ± 0.0
Thr
3.618ThrAla: 3.618 ± 0.359
0.402ThrCys: 0.402 ± 0.095
3.893ThrAsp: 3.893 ± 0.302
5.057ThrGlu: 5.057 ± 0.352
2.349ThrPhe: 2.349 ± 0.25
4.211ThrGly: 4.211 ± 0.352
1.058ThrHis: 1.058 ± 0.133
3.47ThrIle: 3.47 ± 0.329
3.83ThrLys: 3.83 ± 0.28
4.74ThrLeu: 4.74 ± 0.356
1.121ThrMet: 1.121 ± 0.146
2.878ThrAsn: 2.878 ± 0.271
2.201ThrPro: 2.201 ± 0.324
2.433ThrGln: 2.433 ± 0.251
2.73ThrArg: 2.73 ± 0.253
3.809ThrSer: 3.809 ± 0.387
3.597ThrThr: 3.597 ± 0.356
5.332ThrVal: 5.332 ± 0.497
0.571ThrTrp: 0.571 ± 0.108
2.835ThrTyr: 2.835 ± 0.263
0.0ThrXaa: 0.0 ± 0.0
Val
3.915ValAla: 3.915 ± 0.285
0.592ValCys: 0.592 ± 0.114
4.571ValAsp: 4.571 ± 0.289
5.184ValGlu: 5.184 ± 0.308
2.857ValPhe: 2.857 ± 0.279
3.872ValGly: 3.872 ± 0.312
1.693ValHis: 1.693 ± 0.195
3.978ValIle: 3.978 ± 0.269
5.1ValLys: 5.1 ± 0.319
5.438ValLeu: 5.438 ± 0.307
1.418ValMet: 1.418 ± 0.187
3.513ValAsn: 3.513 ± 0.291
2.73ValPro: 2.73 ± 0.275
2.328ValGln: 2.328 ± 0.198
3.195ValArg: 3.195 ± 0.307
5.163ValSer: 5.163 ± 0.345
4.676ValThr: 4.676 ± 0.375
4.888ValVal: 4.888 ± 0.445
0.762ValTrp: 0.762 ± 0.142
3.28ValTyr: 3.28 ± 0.281
0.0ValXaa: 0.0 ± 0.0
Trp
0.825TrpAla: 0.825 ± 0.135
0.085TrpCys: 0.085 ± 0.046
0.635TrpAsp: 0.635 ± 0.107
1.016TrpGlu: 1.016 ± 0.155
0.444TrpPhe: 0.444 ± 0.09
0.487TrpGly: 0.487 ± 0.098
0.19TrpHis: 0.19 ± 0.067
0.592TrpIle: 0.592 ± 0.11
0.973TrpLys: 0.973 ± 0.119
0.846TrpLeu: 0.846 ± 0.129
0.254TrpMet: 0.254 ± 0.07
0.656TrpAsn: 0.656 ± 0.116
0.0TrpPro: 0.0 ± 0.0
0.233TrpGln: 0.233 ± 0.072
0.339TrpArg: 0.339 ± 0.081
0.614TrpSer: 0.614 ± 0.126
0.508TrpThr: 0.508 ± 0.098
0.783TrpVal: 0.783 ± 0.139
0.212TrpTrp: 0.212 ± 0.071
0.55TrpTyr: 0.55 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.772TyrAla: 2.772 ± 0.235
0.487TyrCys: 0.487 ± 0.087
2.73TyrAsp: 2.73 ± 0.218
3.449TyrGlu: 3.449 ± 0.241
1.354TyrPhe: 1.354 ± 0.18
2.708TyrGly: 2.708 ± 0.316
0.846TyrHis: 0.846 ± 0.128
2.73TyrIle: 2.73 ± 0.277
2.984TyrLys: 2.984 ± 0.283
4.063TyrLeu: 4.063 ± 0.26
0.973TyrMet: 0.973 ± 0.116
2.624TyrAsn: 2.624 ± 0.192
1.248TyrPro: 1.248 ± 0.169
1.397TyrGln: 1.397 ± 0.147
2.328TyrArg: 2.328 ± 0.174
2.962TyrSer: 2.962 ± 0.218
2.645TyrThr: 2.645 ± 0.269
3.089TyrVal: 3.089 ± 0.269
0.339TyrTrp: 0.339 ± 0.091
1.545TyrTyr: 1.545 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 243 proteins (47260 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski