Potato latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6RTB2|B6RTB2_9VIRU Movement protein TGB2 OS=Potato latent virus OX=138982 GN=TGB2 PE=3 SV=1
MM1 pKa = 7.86DD2 pKa = 3.8VLVNKK7 pKa = 10.28ALEE10 pKa = 4.22FGFKK14 pKa = 10.23RR15 pKa = 11.84LSSVLKK21 pKa = 10.54SPIVFHH27 pKa = 6.15SVPGAGKK34 pKa = 6.55TTLIRR39 pKa = 11.84ALLNSDD45 pKa = 3.44SRR47 pKa = 11.84FEE49 pKa = 4.04GWTLAEE55 pKa = 4.0GDD57 pKa = 3.96KK58 pKa = 11.12PNLEE62 pKa = 3.85GVVIRR67 pKa = 11.84KK68 pKa = 8.98YY69 pKa = 10.82VGGEE73 pKa = 3.79VGPFALLDD81 pKa = 4.14EE82 pKa = 4.85YY83 pKa = 11.29CVEE86 pKa = 4.13PEE88 pKa = 4.04IAGKK92 pKa = 10.43LYY94 pKa = 10.94AVFGDD99 pKa = 3.8PLQVNNIGFLRR110 pKa = 11.84ASWIKK115 pKa = 10.84VEE117 pKa = 3.82THH119 pKa = 6.47RR120 pKa = 11.84FGKK123 pKa = 8.94ATAQLLNQFGFEE135 pKa = 4.2VSSSKK140 pKa = 11.36ADD142 pKa = 3.22ILVIADD148 pKa = 3.66IFVGEE153 pKa = 4.16PEE155 pKa = 4.09GTVVYY160 pKa = 10.48FEE162 pKa = 4.89EE163 pKa = 5.1EE164 pKa = 4.21VGCLLKK170 pKa = 10.62RR171 pKa = 11.84HH172 pKa = 5.66SLEE175 pKa = 4.02AVHH178 pKa = 6.89IDD180 pKa = 3.81LVRR183 pKa = 11.84GDD185 pKa = 4.22SFPVVTFVTSEE196 pKa = 3.42NCMILDD202 pKa = 3.66KK203 pKa = 11.15VRR205 pKa = 11.84SFNCLTRR212 pKa = 11.84HH213 pKa = 4.91SEE215 pKa = 4.1KK216 pKa = 11.0LIILCPNATYY226 pKa = 10.91SPTT229 pKa = 3.33

Molecular weight: 25.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6RTB0|B6RTB0_9VIRU RdRp polyprotein replicase OS=Potato latent virus OX=138982 GN=RdRp PE=3 SV=1
MM1 pKa = 7.11QPISHH6 pKa = 6.51YY7 pKa = 7.78EE8 pKa = 3.77AKK10 pKa = 10.66LLAVSLAMYY19 pKa = 10.09KK20 pKa = 9.2FTGRR24 pKa = 11.84CEE26 pKa = 3.64PAVALNIVNKK36 pKa = 9.91ACNVGMGKK44 pKa = 10.0SSFARR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84AALLGRR58 pKa = 11.84CHH60 pKa = 6.64RR61 pKa = 11.84CFRR64 pKa = 11.84TSMATRR70 pKa = 11.84CNGVTCYY77 pKa = 10.01PGIGAKK83 pKa = 10.01PKK85 pKa = 9.52IEE87 pKa = 3.99MFIKK91 pKa = 10.76YY92 pKa = 9.18GVSEE96 pKa = 4.46LKK98 pKa = 10.5PP99 pKa = 3.48

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2569

65

1780

428.2

48.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.357 ± 0.826

2.725 ± 0.496

4.165 ± 0.522

7.629 ± 0.966

4.982 ± 0.647

6.072 ± 0.655

2.219 ± 0.186

5.527 ± 0.562

6.773 ± 1.044

9.615 ± 0.816

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.332

4.165 ± 0.451

4.009 ± 1.059

2.725 ± 0.422

5.956 ± 0.685

6.929 ± 0.327

4.749 ± 0.258

7.746 ± 0.684

0.934 ± 0.13

3.387 ± 0.6
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski