Streptococcus phage Javan494

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BBV6|A0A4D6BBV6_9CAUD Cro/CI family transcriptional regulator OS=Streptococcus phage Javan494 OX=2548209 GN=Javan494_0048 PE=4 SV=1
MM1 pKa = 7.64NYY3 pKa = 10.43LEE5 pKa = 4.23YY6 pKa = 11.18ALVYY10 pKa = 10.03LEE12 pKa = 5.12RR13 pKa = 11.84EE14 pKa = 4.11LEE16 pKa = 4.5IIDD19 pKa = 3.9NEE21 pKa = 4.38VIEE24 pKa = 4.3VEE26 pKa = 4.36LPGGDD31 pKa = 3.23WEE33 pKa = 4.69FVPNPYY39 pKa = 9.61YY40 pKa = 10.61EE41 pKa = 5.06KK42 pKa = 10.67GLHH45 pKa = 6.74DD46 pKa = 4.15SPHH49 pKa = 5.83YY50 pKa = 9.94RR51 pKa = 11.84SQVAKK56 pKa = 10.58DD57 pKa = 3.09ILDD60 pKa = 3.46IKK62 pKa = 10.8GLLGRR67 pKa = 4.72

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B6U8|A0A4D6B6U8_9CAUD Integrase OS=Streptococcus phage Javan494 OX=2548209 GN=Javan494_0051 PE=3 SV=1
MM1 pKa = 7.4TKK3 pKa = 10.33NISTFWVKK11 pKa = 10.65NVDD14 pKa = 4.13NSTKK18 pKa = 8.57WVYY21 pKa = 10.68HH22 pKa = 5.88IFCKK26 pKa = 10.46QEE28 pKa = 3.57ITKK31 pKa = 8.81KK32 pKa = 5.97TTKK35 pKa = 8.73QTTIKK40 pKa = 10.32QFWRR44 pKa = 11.84TSLSS48 pKa = 3.26

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12081

38

1460

236.9

26.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.071 ± 0.689

0.464 ± 0.107

6.216 ± 0.458

7.16 ± 0.634

3.683 ± 0.228

6.374 ± 0.416

1.25 ± 0.146

7.135 ± 0.355

8.749 ± 0.558

8.774 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.153

5.413 ± 0.3

2.748 ± 0.213

3.808 ± 0.292

4.271 ± 0.313

6.622 ± 0.706

5.985 ± 0.345

5.976 ± 0.21

1.018 ± 0.107

3.957 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski