Amino acid dipepetide frequency for Vibrio phage VPp1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.14AlaAla: 7.14 ± 1.161
0.714AlaCys: 0.714 ± 0.182
4.414AlaAsp: 4.414 ± 0.603
5.517AlaGlu: 5.517 ± 0.71
2.726AlaPhe: 2.726 ± 0.35
4.998AlaGly: 4.998 ± 0.691
1.233AlaHis: 1.233 ± 0.288
4.738AlaIle: 4.738 ± 0.558
5.777AlaLys: 5.777 ± 0.613
7.464AlaLeu: 7.464 ± 0.904
2.726AlaMet: 2.726 ± 0.566
3.894AlaAsn: 3.894 ± 0.518
2.596AlaPro: 2.596 ± 0.468
3.894AlaGln: 3.894 ± 0.649
3.7AlaArg: 3.7 ± 0.421
3.959AlaSer: 3.959 ± 0.578
4.543AlaThr: 4.543 ± 0.465
5.128AlaVal: 5.128 ± 0.561
0.974AlaTrp: 0.974 ± 0.26
2.921AlaTyr: 2.921 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.519CysAla: 0.519 ± 0.158
0.325CysCys: 0.325 ± 0.255
0.714CysAsp: 0.714 ± 0.263
0.844CysGlu: 0.844 ± 0.247
0.454CysPhe: 0.454 ± 0.161
1.233CysGly: 1.233 ± 0.313
0.26CysHis: 0.26 ± 0.119
0.649CysIle: 0.649 ± 0.24
0.909CysLys: 0.909 ± 0.257
0.714CysLeu: 0.714 ± 0.248
0.195CysMet: 0.195 ± 0.125
0.844CysAsn: 0.844 ± 0.278
0.26CysPro: 0.26 ± 0.109
0.26CysGln: 0.26 ± 0.149
0.779CysArg: 0.779 ± 0.225
0.389CysSer: 0.389 ± 0.134
0.779CysThr: 0.779 ± 0.249
0.649CysVal: 0.649 ± 0.185
0.195CysTrp: 0.195 ± 0.121
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.803AspAla: 4.803 ± 0.636
1.038AspCys: 1.038 ± 0.282
4.024AspAsp: 4.024 ± 0.613
5.517AspGlu: 5.517 ± 0.704
2.466AspPhe: 2.466 ± 0.476
6.036AspGly: 6.036 ± 0.95
1.168AspHis: 1.168 ± 0.287
3.57AspIle: 3.57 ± 0.435
5.387AspLys: 5.387 ± 0.573
4.868AspLeu: 4.868 ± 0.578
1.947AspMet: 1.947 ± 0.42
3.375AspAsn: 3.375 ± 0.44
2.726AspPro: 2.726 ± 0.39
1.363AspGln: 1.363 ± 0.29
2.012AspArg: 2.012 ± 0.351
3.115AspSer: 3.115 ± 0.431
3.765AspThr: 3.765 ± 0.513
3.829AspVal: 3.829 ± 0.495
1.233AspTrp: 1.233 ± 0.26
2.726AspTyr: 2.726 ± 0.432
0.0AspXaa: 0.0 ± 0.0
Glu
6.491GluAla: 6.491 ± 0.801
0.454GluCys: 0.454 ± 0.143
3.959GluAsp: 3.959 ± 0.596
7.659GluGlu: 7.659 ± 1.5
2.791GluPhe: 2.791 ± 0.408
3.894GluGly: 3.894 ± 0.446
1.688GluHis: 1.688 ± 0.374
4.089GluIle: 4.089 ± 0.47
4.998GluLys: 4.998 ± 0.611
7.724GluLeu: 7.724 ± 0.789
1.882GluMet: 1.882 ± 0.385
2.596GluAsn: 2.596 ± 0.353
2.661GluPro: 2.661 ± 0.426
3.44GluGln: 3.44 ± 0.555
3.765GluArg: 3.765 ± 0.422
2.986GluSer: 2.986 ± 0.596
3.44GluThr: 3.44 ± 0.511
5.192GluVal: 5.192 ± 0.598
1.168GluTrp: 1.168 ± 0.265
3.44GluTyr: 3.44 ± 0.486
0.0GluXaa: 0.0 ± 0.0
Phe
2.921PheAla: 2.921 ± 0.395
0.325PheCys: 0.325 ± 0.132
2.986PheAsp: 2.986 ± 0.424
2.272PheGlu: 2.272 ± 0.324
1.363PhePhe: 1.363 ± 0.371
2.856PheGly: 2.856 ± 0.425
0.649PheHis: 0.649 ± 0.208
2.142PheIle: 2.142 ± 0.359
3.051PheLys: 3.051 ± 0.557
2.402PheLeu: 2.402 ± 0.294
1.103PheMet: 1.103 ± 0.305
2.921PheAsn: 2.921 ± 0.537
1.493PhePro: 1.493 ± 0.319
1.103PheGln: 1.103 ± 0.262
1.038PheArg: 1.038 ± 0.299
2.402PheSer: 2.402 ± 0.375
1.817PheThr: 1.817 ± 0.362
3.051PheVal: 3.051 ± 0.376
0.584PheTrp: 0.584 ± 0.178
1.233PheTyr: 1.233 ± 0.288
0.0PheXaa: 0.0 ± 0.0
Gly
5.971GlyAla: 5.971 ± 1.06
1.103GlyCys: 1.103 ± 0.333
3.57GlyAsp: 3.57 ± 0.337
4.543GlyGlu: 4.543 ± 0.529
3.18GlyPhe: 3.18 ± 0.461
7.399GlyGly: 7.399 ± 1.356
1.428GlyHis: 1.428 ± 0.376
4.543GlyIle: 4.543 ± 0.607
5.387GlyLys: 5.387 ± 0.549
5.128GlyLeu: 5.128 ± 0.803
1.752GlyMet: 1.752 ± 0.308
4.608GlyAsn: 4.608 ± 0.449
0.584GlyPro: 0.584 ± 0.274
2.531GlyGln: 2.531 ± 0.48
3.115GlyArg: 3.115 ± 0.476
4.998GlySer: 4.998 ± 0.64
4.219GlyThr: 4.219 ± 0.518
4.998GlyVal: 4.998 ± 0.795
0.779GlyTrp: 0.779 ± 0.193
3.245GlyTyr: 3.245 ± 0.477
0.0GlyXaa: 0.0 ± 0.0
His
1.298HisAla: 1.298 ± 0.308
0.454HisCys: 0.454 ± 0.178
1.233HisAsp: 1.233 ± 0.275
0.844HisGlu: 0.844 ± 0.257
0.714HisPhe: 0.714 ± 0.285
1.688HisGly: 1.688 ± 0.343
0.714HisHis: 0.714 ± 0.239
1.493HisIle: 1.493 ± 0.293
1.168HisLys: 1.168 ± 0.282
1.688HisLeu: 1.688 ± 0.348
1.038HisMet: 1.038 ± 0.221
1.233HisAsn: 1.233 ± 0.391
0.844HisPro: 0.844 ± 0.273
0.844HisGln: 0.844 ± 0.251
1.103HisArg: 1.103 ± 0.237
0.649HisSer: 0.649 ± 0.203
1.493HisThr: 1.493 ± 0.324
0.974HisVal: 0.974 ± 0.24
0.325HisTrp: 0.325 ± 0.154
1.168HisTyr: 1.168 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
3.57IleAla: 3.57 ± 0.505
0.714IleCys: 0.714 ± 0.179
4.868IleAsp: 4.868 ± 0.559
3.31IleGlu: 3.31 ± 0.435
1.947IlePhe: 1.947 ± 0.362
3.635IleGly: 3.635 ± 0.548
1.168IleHis: 1.168 ± 0.23
2.661IleIle: 2.661 ± 0.412
4.543IleLys: 4.543 ± 0.559
3.57IleLeu: 3.57 ± 0.51
1.298IleMet: 1.298 ± 0.244
2.466IleAsn: 2.466 ± 0.3
2.077IlePro: 2.077 ± 0.37
2.661IleGln: 2.661 ± 0.323
2.337IleArg: 2.337 ± 0.375
2.726IleSer: 2.726 ± 0.411
3.115IleThr: 3.115 ± 0.53
4.089IleVal: 4.089 ± 0.497
0.714IleTrp: 0.714 ± 0.203
1.752IleTyr: 1.752 ± 0.283
0.0IleXaa: 0.0 ± 0.0
Lys
6.361LysAla: 6.361 ± 0.705
0.649LysCys: 0.649 ± 0.235
4.868LysAsp: 4.868 ± 0.542
5.192LysGlu: 5.192 ± 0.68
2.596LysPhe: 2.596 ± 0.496
4.803LysGly: 4.803 ± 0.47
1.947LysHis: 1.947 ± 0.399
3.051LysIle: 3.051 ± 0.406
5.192LysLys: 5.192 ± 0.734
5.971LysLeu: 5.971 ± 0.707
2.077LysMet: 2.077 ± 0.369
2.661LysAsn: 2.661 ± 0.452
3.375LysPro: 3.375 ± 0.488
3.245LysGln: 3.245 ± 0.489
4.024LysArg: 4.024 ± 0.562
2.986LysSer: 2.986 ± 0.462
4.219LysThr: 4.219 ± 0.588
4.933LysVal: 4.933 ± 0.481
0.909LysTrp: 0.909 ± 0.214
3.31LysTyr: 3.31 ± 0.54
0.0LysXaa: 0.0 ± 0.0
Leu
6.815LeuAla: 6.815 ± 0.795
0.714LeuCys: 0.714 ± 0.228
5.322LeuAsp: 5.322 ± 0.432
5.777LeuGlu: 5.777 ± 0.677
2.791LeuPhe: 2.791 ± 0.61
5.777LeuGly: 5.777 ± 0.727
1.493LeuHis: 1.493 ± 0.312
3.765LeuIle: 3.765 ± 0.423
4.933LeuLys: 4.933 ± 0.621
4.608LeuLeu: 4.608 ± 0.651
2.531LeuMet: 2.531 ± 0.483
4.414LeuAsn: 4.414 ± 0.622
2.596LeuPro: 2.596 ± 0.434
3.375LeuGln: 3.375 ± 0.526
4.154LeuArg: 4.154 ± 0.522
4.738LeuSer: 4.738 ± 0.603
5.517LeuThr: 5.517 ± 0.7
4.543LeuVal: 4.543 ± 0.469
0.649LeuTrp: 0.649 ± 0.19
2.531LeuTyr: 2.531 ± 0.461
0.0LeuXaa: 0.0 ± 0.0
Met
2.921MetAla: 2.921 ± 0.419
0.454MetCys: 0.454 ± 0.156
1.558MetAsp: 1.558 ± 0.304
2.142MetGlu: 2.142 ± 0.395
1.233MetPhe: 1.233 ± 0.347
1.623MetGly: 1.623 ± 0.335
0.584MetHis: 0.584 ± 0.183
1.038MetIle: 1.038 ± 0.266
2.207MetLys: 2.207 ± 0.387
1.688MetLeu: 1.688 ± 0.305
0.974MetMet: 0.974 ± 0.231
1.623MetAsn: 1.623 ± 0.33
1.363MetPro: 1.363 ± 0.275
1.298MetGln: 1.298 ± 0.332
1.103MetArg: 1.103 ± 0.204
2.142MetSer: 2.142 ± 0.326
1.623MetThr: 1.623 ± 0.426
1.428MetVal: 1.428 ± 0.262
0.195MetTrp: 0.195 ± 0.1
0.909MetTyr: 0.909 ± 0.273
0.0MetXaa: 0.0 ± 0.0
Asn
4.219AsnAla: 4.219 ± 0.566
0.454AsnCys: 0.454 ± 0.198
3.57AsnAsp: 3.57 ± 0.471
2.986AsnGlu: 2.986 ± 0.448
1.947AsnPhe: 1.947 ± 0.457
4.803AsnGly: 4.803 ± 0.633
0.909AsnHis: 0.909 ± 0.264
2.791AsnIle: 2.791 ± 0.383
3.829AsnLys: 3.829 ± 0.577
4.219AsnLeu: 4.219 ± 0.497
1.298AsnMet: 1.298 ± 0.358
3.765AsnAsn: 3.765 ± 0.63
2.466AsnPro: 2.466 ± 0.374
1.817AsnGln: 1.817 ± 0.341
2.986AsnArg: 2.986 ± 0.504
2.726AsnSer: 2.726 ± 0.403
3.115AsnThr: 3.115 ± 0.579
2.921AsnVal: 2.921 ± 0.446
0.974AsnTrp: 0.974 ± 0.248
2.077AsnTyr: 2.077 ± 0.399
0.0AsnXaa: 0.0 ± 0.0
Pro
2.466ProAla: 2.466 ± 0.434
0.26ProCys: 0.26 ± 0.117
2.466ProAsp: 2.466 ± 0.531
3.051ProGlu: 3.051 ± 0.506
1.752ProPhe: 1.752 ± 0.327
0.584ProGly: 0.584 ± 0.201
1.168ProHis: 1.168 ± 0.342
1.558ProIle: 1.558 ± 0.322
2.986ProLys: 2.986 ± 0.422
2.856ProLeu: 2.856 ± 0.411
1.298ProMet: 1.298 ± 0.265
2.466ProAsn: 2.466 ± 0.387
1.038ProPro: 1.038 ± 0.348
1.882ProGln: 1.882 ± 0.358
1.493ProArg: 1.493 ± 0.268
1.558ProSer: 1.558 ± 0.506
2.791ProThr: 2.791 ± 0.5
2.856ProVal: 2.856 ± 0.372
0.714ProTrp: 0.714 ± 0.185
0.974ProTyr: 0.974 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
4.024GlnAla: 4.024 ± 0.551
0.389GlnCys: 0.389 ± 0.164
2.337GlnAsp: 2.337 ± 0.411
3.894GlnGlu: 3.894 ± 0.681
1.363GlnPhe: 1.363 ± 0.265
2.337GlnGly: 2.337 ± 0.404
0.844GlnHis: 0.844 ± 0.251
2.272GlnIle: 2.272 ± 0.399
1.817GlnLys: 1.817 ± 0.432
3.505GlnLeu: 3.505 ± 0.737
1.233GlnMet: 1.233 ± 0.235
2.012GlnAsn: 2.012 ± 0.346
1.298GlnPro: 1.298 ± 0.243
1.947GlnGln: 1.947 ± 0.456
1.947GlnArg: 1.947 ± 0.32
2.466GlnSer: 2.466 ± 0.452
2.142GlnThr: 2.142 ± 0.34
2.596GlnVal: 2.596 ± 0.394
0.909GlnTrp: 0.909 ± 0.213
1.558GlnTyr: 1.558 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
2.921ArgAla: 2.921 ± 0.445
0.649ArgCys: 0.649 ± 0.19
3.31ArgAsp: 3.31 ± 0.536
3.894ArgGlu: 3.894 ± 0.507
1.752ArgPhe: 1.752 ± 0.375
2.791ArgGly: 2.791 ± 0.388
1.103ArgHis: 1.103 ± 0.273
2.661ArgIle: 2.661 ± 0.363
3.57ArgLys: 3.57 ± 0.592
3.44ArgLeu: 3.44 ± 0.538
1.233ArgMet: 1.233 ± 0.249
2.726ArgAsn: 2.726 ± 0.49
1.428ArgPro: 1.428 ± 0.258
1.752ArgGln: 1.752 ± 0.436
1.947ArgArg: 1.947 ± 0.386
1.752ArgSer: 1.752 ± 0.292
2.856ArgThr: 2.856 ± 0.651
3.115ArgVal: 3.115 ± 0.43
0.844ArgTrp: 0.844 ± 0.252
1.882ArgTyr: 1.882 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
3.57SerAla: 3.57 ± 0.652
0.26SerCys: 0.26 ± 0.147
3.505SerAsp: 3.505 ± 0.411
3.894SerGlu: 3.894 ± 0.52
1.623SerPhe: 1.623 ± 0.261
4.998SerGly: 4.998 ± 0.792
1.168SerHis: 1.168 ± 0.279
2.921SerIle: 2.921 ± 0.447
3.115SerLys: 3.115 ± 0.421
3.829SerLeu: 3.829 ± 0.49
1.363SerMet: 1.363 ± 0.297
2.402SerAsn: 2.402 ± 0.425
1.493SerPro: 1.493 ± 0.31
2.207SerGln: 2.207 ± 0.413
3.505SerArg: 3.505 ± 0.442
2.921SerSer: 2.921 ± 0.465
3.505SerThr: 3.505 ± 0.693
3.635SerVal: 3.635 ± 0.468
0.844SerTrp: 0.844 ± 0.253
2.077SerTyr: 2.077 ± 0.432
0.0SerXaa: 0.0 ± 0.0
Thr
5.257ThrAla: 5.257 ± 0.558
0.519ThrCys: 0.519 ± 0.185
3.31ThrAsp: 3.31 ± 0.455
3.765ThrGlu: 3.765 ± 0.47
2.402ThrPhe: 2.402 ± 0.348
5.777ThrGly: 5.777 ± 0.671
0.909ThrHis: 0.909 ± 0.231
3.31ThrIle: 3.31 ± 0.517
4.024ThrLys: 4.024 ± 0.641
4.998ThrLeu: 4.998 ± 0.635
1.233ThrMet: 1.233 ± 0.332
3.051ThrAsn: 3.051 ± 0.438
3.115ThrPro: 3.115 ± 0.541
2.466ThrGln: 2.466 ± 0.376
2.077ThrArg: 2.077 ± 0.341
2.921ThrSer: 2.921 ± 0.75
3.635ThrThr: 3.635 ± 0.5
3.635ThrVal: 3.635 ± 0.468
0.649ThrTrp: 0.649 ± 0.265
2.337ThrTyr: 2.337 ± 0.431
0.0ThrXaa: 0.0 ± 0.0
Val
4.738ValAla: 4.738 ± 0.51
0.844ValCys: 0.844 ± 0.249
4.738ValAsp: 4.738 ± 0.65
5.582ValGlu: 5.582 ± 0.465
2.531ValPhe: 2.531 ± 0.431
3.894ValGly: 3.894 ± 0.574
1.363ValHis: 1.363 ± 0.253
3.115ValIle: 3.115 ± 0.498
5.387ValLys: 5.387 ± 0.577
3.635ValLeu: 3.635 ± 0.38
1.882ValMet: 1.882 ± 0.364
2.596ValAsn: 2.596 ± 0.534
3.115ValPro: 3.115 ± 0.449
2.466ValGln: 2.466 ± 0.331
2.077ValArg: 2.077 ± 0.308
4.349ValSer: 4.349 ± 0.51
4.089ValThr: 4.089 ± 0.614
4.998ValVal: 4.998 ± 0.549
1.752ValTrp: 1.752 ± 0.437
2.596ValTyr: 2.596 ± 0.462
0.0ValXaa: 0.0 ± 0.0
Trp
0.714TrpAla: 0.714 ± 0.218
0.26TrpCys: 0.26 ± 0.149
1.103TrpAsp: 1.103 ± 0.311
1.168TrpGlu: 1.168 ± 0.326
0.779TrpPhe: 0.779 ± 0.229
1.038TrpGly: 1.038 ± 0.248
0.195TrpHis: 0.195 ± 0.106
0.779TrpIle: 0.779 ± 0.235
1.363TrpLys: 1.363 ± 0.309
1.103TrpLeu: 1.103 ± 0.246
0.325TrpMet: 0.325 ± 0.139
0.974TrpAsn: 0.974 ± 0.269
0.13TrpPro: 0.13 ± 0.089
0.649TrpGln: 0.649 ± 0.204
0.974TrpArg: 0.974 ± 0.209
0.844TrpSer: 0.844 ± 0.238
0.909TrpThr: 0.909 ± 0.263
1.168TrpVal: 1.168 ± 0.241
0.325TrpTrp: 0.325 ± 0.167
0.519TrpTyr: 0.519 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.337TyrAla: 2.337 ± 0.36
0.389TyrCys: 0.389 ± 0.18
3.115TyrAsp: 3.115 ± 0.534
2.466TyrGlu: 2.466 ± 0.561
1.298TyrPhe: 1.298 ± 0.263
2.921TyrGly: 2.921 ± 0.408
0.974TyrHis: 0.974 ± 0.244
2.077TyrIle: 2.077 ± 0.372
2.726TyrLys: 2.726 ± 0.457
3.635TyrLeu: 3.635 ± 0.571
0.649TyrMet: 0.649 ± 0.27
3.245TyrAsn: 3.245 ± 0.451
1.558TyrPro: 1.558 ± 0.251
1.688TyrGln: 1.688 ± 0.32
1.493TyrArg: 1.493 ± 0.325
2.207TyrSer: 2.207 ± 0.465
1.817TyrThr: 1.817 ± 0.314
2.012TyrVal: 2.012 ± 0.355
0.584TyrTrp: 0.584 ± 0.25
1.103TyrTyr: 1.103 ± 0.351
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (15408 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski