Streptococcus phage Javan207

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A5M2|A0A4D6A5M2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan207 OX=2548047 GN=Javan207_0031 PE=4 SV=1
MM1 pKa = 7.26VKK3 pKa = 10.35LRR5 pKa = 11.84DD6 pKa = 4.57FINLIIMDD14 pKa = 4.74NNDD17 pKa = 4.25PIYY20 pKa = 10.49IFEE23 pKa = 5.6DD24 pKa = 3.09NHH26 pKa = 7.76KK27 pKa = 8.53MLEE30 pKa = 4.12YY31 pKa = 10.73GVDD34 pKa = 3.63DD35 pKa = 4.26VSDD38 pKa = 3.64VLSYY42 pKa = 11.33YY43 pKa = 10.01IDD45 pKa = 3.86TIVIYY50 pKa = 10.73DD51 pKa = 3.78GMVEE55 pKa = 3.86ITLKK59 pKa = 11.06EE60 pKa = 4.03EE61 pKa = 4.06LL62 pKa = 4.03

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A2Z8|A0A4D6A2Z8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan207 OX=2548047 GN=Javan207_0049 PE=4 SV=1
MM1 pKa = 7.35QDD3 pKa = 2.81KK4 pKa = 10.19KK5 pKa = 10.86RR6 pKa = 11.84PRR8 pKa = 11.84GRR10 pKa = 11.84PATGRR15 pKa = 11.84EE16 pKa = 3.99RR17 pKa = 11.84NISMTMRR24 pKa = 11.84VTEE27 pKa = 4.34KK28 pKa = 9.92EE29 pKa = 3.76RR30 pKa = 11.84EE31 pKa = 4.12IIKK34 pKa = 10.51LSQKK38 pKa = 7.97EE39 pKa = 4.18HH40 pKa = 5.72NKK42 pKa = 10.26KK43 pKa = 10.65SVVDD47 pKa = 4.21LLLHH51 pKa = 6.88FIEE54 pKa = 4.92KK55 pKa = 10.51NKK57 pKa = 10.73

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11747

39

1444

189.5

21.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.436 ± 0.677

0.621 ± 0.121

6.589 ± 0.363

8.053 ± 0.536

3.984 ± 0.396

6.334 ± 0.456

1.354 ± 0.115

6.359 ± 0.295

8.658 ± 0.408

8.028 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.201

5.865 ± 0.222

2.528 ± 0.148

3.626 ± 0.316

3.797 ± 0.362

6.41 ± 0.315

6.495 ± 0.667

6.359 ± 0.235

1.319 ± 0.182

4.273 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski