Amino acid dipepetide frequency for Enterobacter phage EspM4VN

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.402AlaAla: 6.402 ± 0.453
0.785AlaCys: 0.785 ± 0.121
4.75AlaAsp: 4.75 ± 0.292
4.812AlaGlu: 4.812 ± 0.386
2.519AlaPhe: 2.519 ± 0.222
5.307AlaGly: 5.307 ± 0.315
1.28AlaHis: 1.28 ± 0.156
4.791AlaIle: 4.791 ± 0.304
4.502AlaLys: 4.502 ± 0.312
6.278AlaLeu: 6.278 ± 0.376
2.065AlaMet: 2.065 ± 0.197
2.912AlaAsn: 2.912 ± 0.244
2.829AlaPro: 2.829 ± 0.245
2.705AlaGln: 2.705 ± 0.248
3.635AlaArg: 3.635 ± 0.308
4.192AlaSer: 4.192 ± 0.326
4.564AlaThr: 4.564 ± 0.352
5.08AlaVal: 5.08 ± 0.338
1.094AlaTrp: 1.094 ± 0.155
2.664AlaTyr: 2.664 ± 0.27
0.0AlaXaa: 0.0 ± 0.0
Cys
0.785CysAla: 0.785 ± 0.149
0.145CysCys: 0.145 ± 0.055
0.702CysAsp: 0.702 ± 0.142
0.826CysGlu: 0.826 ± 0.129
0.372CysPhe: 0.372 ± 0.088
0.64CysGly: 0.64 ± 0.135
0.413CysHis: 0.413 ± 0.097
0.743CysIle: 0.743 ± 0.115
0.681CysLys: 0.681 ± 0.126
0.826CysLeu: 0.826 ± 0.146
0.268CysMet: 0.268 ± 0.075
0.599CysAsn: 0.599 ± 0.115
0.578CysPro: 0.578 ± 0.103
0.207CysGln: 0.207 ± 0.059
0.516CysArg: 0.516 ± 0.105
0.991CysSer: 0.991 ± 0.128
0.743CysThr: 0.743 ± 0.13
1.115CysVal: 1.115 ± 0.164
0.186CysTrp: 0.186 ± 0.059
0.372CysTyr: 0.372 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
4.894AspAla: 4.894 ± 0.398
0.64AspCys: 0.64 ± 0.13
4.233AspAsp: 4.233 ± 0.373
4.316AspGlu: 4.316 ± 0.339
3.511AspPhe: 3.511 ± 0.26
5.163AspGly: 5.163 ± 0.293
0.991AspHis: 0.991 ± 0.142
4.77AspIle: 4.77 ± 0.27
3.635AspLys: 3.635 ± 0.291
5.596AspLeu: 5.596 ± 0.325
1.817AspMet: 1.817 ± 0.187
2.891AspAsn: 2.891 ± 0.303
2.891AspPro: 2.891 ± 0.229
1.921AspGln: 1.921 ± 0.208
2.829AspArg: 2.829 ± 0.3
3.18AspSer: 3.18 ± 0.242
3.655AspThr: 3.655 ± 0.288
4.192AspVal: 4.192 ± 0.277
1.198AspTrp: 1.198 ± 0.173
3.139AspTyr: 3.139 ± 0.273
0.0AspXaa: 0.0 ± 0.0
Glu
4.564GluAla: 4.564 ± 0.398
0.702GluCys: 0.702 ± 0.134
4.295GluAsp: 4.295 ± 0.388
4.44GluGlu: 4.44 ± 0.349
3.139GluPhe: 3.139 ± 0.257
4.068GluGly: 4.068 ± 0.277
1.218GluHis: 1.218 ± 0.149
3.614GluIle: 3.614 ± 0.278
3.18GluLys: 3.18 ± 0.339
6.175GluLeu: 6.175 ± 0.398
2.23GluMet: 2.23 ± 0.208
2.912GluAsn: 2.912 ± 0.25
2.086GluPro: 2.086 ± 0.217
2.623GluGln: 2.623 ± 0.238
3.676GluArg: 3.676 ± 0.291
3.015GluSer: 3.015 ± 0.287
3.758GluThr: 3.758 ± 0.293
4.936GluVal: 4.936 ± 0.316
1.012GluTrp: 1.012 ± 0.134
2.829GluTyr: 2.829 ± 0.267
0.0GluXaa: 0.0 ± 0.0
Phe
2.375PheAla: 2.375 ± 0.257
0.454PheCys: 0.454 ± 0.113
2.705PheAsp: 2.705 ± 0.243
3.263PheGlu: 3.263 ± 0.259
1.611PhePhe: 1.611 ± 0.223
3.18PheGly: 3.18 ± 0.281
0.888PheHis: 0.888 ± 0.127
2.747PheIle: 2.747 ± 0.228
2.643PheLys: 2.643 ± 0.196
3.098PheLeu: 3.098 ± 0.247
1.446PheMet: 1.446 ± 0.183
2.623PheAsn: 2.623 ± 0.239
1.673PhePro: 1.673 ± 0.245
1.631PheGln: 1.631 ± 0.218
2.251PheArg: 2.251 ± 0.246
2.664PheSer: 2.664 ± 0.226
2.953PheThr: 2.953 ± 0.267
2.747PheVal: 2.747 ± 0.255
0.723PheTrp: 0.723 ± 0.127
1.693PheTyr: 1.693 ± 0.186
0.0PheXaa: 0.0 ± 0.0
Gly
4.667GlyAla: 4.667 ± 0.408
0.929GlyCys: 0.929 ± 0.14
3.697GlyAsp: 3.697 ± 0.29
4.419GlyGlu: 4.419 ± 0.27
3.098GlyPhe: 3.098 ± 0.289
5.101GlyGly: 5.101 ± 0.556
1.631GlyHis: 1.631 ± 0.177
4.729GlyIle: 4.729 ± 0.328
4.75GlyLys: 4.75 ± 0.334
5.245GlyLeu: 5.245 ± 0.282
1.921GlyMet: 1.921 ± 0.227
3.387GlyAsn: 3.387 ± 0.363
1.26GlyPro: 1.26 ± 0.146
2.519GlyGln: 2.519 ± 0.221
3.242GlyArg: 3.242 ± 0.266
4.295GlySer: 4.295 ± 0.401
4.151GlyThr: 4.151 ± 0.359
5.39GlyVal: 5.39 ± 0.322
1.115GlyTrp: 1.115 ± 0.156
2.437GlyTyr: 2.437 ± 0.214
0.0GlyXaa: 0.0 ± 0.0
His
1.115HisAla: 1.115 ± 0.154
0.268HisCys: 0.268 ± 0.076
1.239HisAsp: 1.239 ± 0.203
0.847HisGlu: 0.847 ± 0.165
0.909HisPhe: 0.909 ± 0.126
1.26HisGly: 1.26 ± 0.179
0.537HisHis: 0.537 ± 0.102
1.28HisIle: 1.28 ± 0.196
1.28HisLys: 1.28 ± 0.188
1.59HisLeu: 1.59 ± 0.189
0.599HisMet: 0.599 ± 0.117
0.764HisAsn: 0.764 ± 0.148
1.094HisPro: 1.094 ± 0.161
0.599HisGln: 0.599 ± 0.113
1.012HisArg: 1.012 ± 0.133
1.053HisSer: 1.053 ± 0.144
1.094HisThr: 1.094 ± 0.162
1.301HisVal: 1.301 ± 0.204
0.227HisTrp: 0.227 ± 0.075
0.826HisTyr: 0.826 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
4.048IleAla: 4.048 ± 0.308
0.805IleCys: 0.805 ± 0.124
4.956IleAsp: 4.956 ± 0.287
4.543IleGlu: 4.543 ± 0.363
1.652IlePhe: 1.652 ± 0.187
3.573IleGly: 3.573 ± 0.245
1.198IleHis: 1.198 ± 0.174
2.953IleIle: 2.953 ± 0.258
3.593IleLys: 3.593 ± 0.298
4.213IleLeu: 4.213 ± 0.273
1.425IleMet: 1.425 ± 0.205
3.16IleAsn: 3.16 ± 0.256
2.829IlePro: 2.829 ± 0.232
2.685IleGln: 2.685 ± 0.21
2.932IleArg: 2.932 ± 0.248
3.263IleSer: 3.263 ± 0.303
4.192IleThr: 4.192 ± 0.271
4.316IleVal: 4.316 ± 0.274
0.62IleTrp: 0.62 ± 0.124
1.9IleTyr: 1.9 ± 0.172
0.0IleXaa: 0.0 ± 0.0
Lys
4.316LysAla: 4.316 ± 0.337
0.558LysCys: 0.558 ± 0.137
4.254LysAsp: 4.254 ± 0.312
4.171LysGlu: 4.171 ± 0.319
2.994LysPhe: 2.994 ± 0.233
3.903LysGly: 3.903 ± 0.318
1.239LysHis: 1.239 ± 0.156
3.345LysIle: 3.345 ± 0.312
3.882LysLys: 3.882 ± 0.353
5.142LysLeu: 5.142 ± 0.358
2.602LysMet: 2.602 ± 0.248
2.726LysAsn: 2.726 ± 0.238
2.685LysPro: 2.685 ± 0.248
2.499LysGln: 2.499 ± 0.254
3.036LysArg: 3.036 ± 0.302
3.469LysSer: 3.469 ± 0.252
3.635LysThr: 3.635 ± 0.265
4.316LysVal: 4.316 ± 0.339
1.033LysTrp: 1.033 ± 0.154
1.982LysTyr: 1.982 ± 0.232
0.0LysXaa: 0.0 ± 0.0
Leu
6.422LeuAla: 6.422 ± 0.367
0.681LeuCys: 0.681 ± 0.124
5.452LeuAsp: 5.452 ± 0.344
4.894LeuGlu: 4.894 ± 0.326
3.655LeuPhe: 3.655 ± 0.28
5.431LeuGly: 5.431 ± 0.36
1.404LeuHis: 1.404 ± 0.172
3.738LeuIle: 3.738 ± 0.257
6.278LeuLys: 6.278 ± 0.382
5.906LeuLeu: 5.906 ± 0.375
2.106LeuMet: 2.106 ± 0.208
4.481LeuAsn: 4.481 ± 0.322
3.697LeuPro: 3.697 ± 0.258
2.705LeuGln: 2.705 ± 0.278
3.986LeuArg: 3.986 ± 0.295
5.204LeuSer: 5.204 ± 0.293
4.977LeuThr: 4.977 ± 0.326
6.133LeuVal: 6.133 ± 0.374
0.785LeuTrp: 0.785 ± 0.133
3.015LeuTyr: 3.015 ± 0.229
0.0LeuXaa: 0.0 ± 0.0
Met
2.664MetAla: 2.664 ± 0.235
0.268MetCys: 0.268 ± 0.083
1.631MetAsp: 1.631 ± 0.159
1.404MetGlu: 1.404 ± 0.175
1.611MetPhe: 1.611 ± 0.181
1.693MetGly: 1.693 ± 0.185
0.475MetHis: 0.475 ± 0.101
1.425MetIle: 1.425 ± 0.16
2.23MetLys: 2.23 ± 0.187
2.561MetLeu: 2.561 ± 0.245
0.743MetMet: 0.743 ± 0.142
1.508MetAsn: 1.508 ± 0.192
1.156MetPro: 1.156 ± 0.163
1.136MetGln: 1.136 ± 0.131
1.755MetArg: 1.755 ± 0.194
2.003MetSer: 2.003 ± 0.229
1.797MetThr: 1.797 ± 0.192
1.921MetVal: 1.921 ± 0.199
0.289MetTrp: 0.289 ± 0.072
0.826MetTyr: 0.826 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
4.006AsnAla: 4.006 ± 0.34
0.681AsnCys: 0.681 ± 0.131
2.499AsnAsp: 2.499 ± 0.22
2.272AsnGlu: 2.272 ± 0.232
2.313AsnPhe: 2.313 ± 0.236
4.337AsnGly: 4.337 ± 0.356
0.971AsnHis: 0.971 ± 0.153
2.809AsnIle: 2.809 ± 0.273
2.994AsnLys: 2.994 ± 0.2
3.882AsnLeu: 3.882 ± 0.282
1.446AsnMet: 1.446 ± 0.161
2.499AsnAsn: 2.499 ± 0.232
2.189AsnPro: 2.189 ± 0.215
1.652AsnGln: 1.652 ± 0.186
2.685AsnArg: 2.685 ± 0.237
2.23AsnSer: 2.23 ± 0.171
3.263AsnThr: 3.263 ± 0.37
3.924AsnVal: 3.924 ± 0.346
0.661AsnTrp: 0.661 ± 0.12
1.962AsnTyr: 1.962 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
2.829ProAla: 2.829 ± 0.219
0.454ProCys: 0.454 ± 0.101
3.263ProAsp: 3.263 ± 0.26
3.098ProGlu: 3.098 ± 0.259
1.776ProPhe: 1.776 ± 0.225
2.292ProGly: 2.292 ± 0.211
0.661ProHis: 0.661 ± 0.111
1.879ProIle: 1.879 ± 0.228
2.457ProLys: 2.457 ± 0.225
3.118ProLeu: 3.118 ± 0.22
0.929ProMet: 0.929 ± 0.122
1.838ProAsn: 1.838 ± 0.19
1.074ProPro: 1.074 ± 0.163
1.446ProGln: 1.446 ± 0.18
1.797ProArg: 1.797 ± 0.194
3.015ProSer: 3.015 ± 0.277
2.602ProThr: 2.602 ± 0.252
3.304ProVal: 3.304 ± 0.256
0.516ProTrp: 0.516 ± 0.099
1.301ProTyr: 1.301 ± 0.185
0.0ProXaa: 0.0 ± 0.0
Gln
2.664GlnAla: 2.664 ± 0.281
0.33GlnCys: 0.33 ± 0.081
2.519GlnAsp: 2.519 ± 0.216
2.21GlnGlu: 2.21 ± 0.339
1.817GlnPhe: 1.817 ± 0.202
2.251GlnGly: 2.251 ± 0.206
0.578GlnHis: 0.578 ± 0.107
2.251GlnIle: 2.251 ± 0.199
2.313GlnLys: 2.313 ± 0.239
2.829GlnLeu: 2.829 ± 0.245
1.115GlnMet: 1.115 ± 0.17
1.508GlnAsn: 1.508 ± 0.177
1.136GlnPro: 1.136 ± 0.134
1.755GlnGln: 1.755 ± 0.301
1.879GlnArg: 1.879 ± 0.206
2.21GlnSer: 2.21 ± 0.24
2.272GlnThr: 2.272 ± 0.195
2.829GlnVal: 2.829 ± 0.237
0.434GlnTrp: 0.434 ± 0.102
1.673GlnTyr: 1.673 ± 0.163
0.0GlnXaa: 0.0 ± 0.0
Arg
3.056ArgAla: 3.056 ± 0.254
0.826ArgCys: 0.826 ± 0.137
3.139ArgAsp: 3.139 ± 0.286
3.077ArgGlu: 3.077 ± 0.311
2.251ArgPhe: 2.251 ± 0.246
3.118ArgGly: 3.118 ± 0.238
0.991ArgHis: 0.991 ± 0.157
2.912ArgIle: 2.912 ± 0.217
3.077ArgLys: 3.077 ± 0.244
4.688ArgLeu: 4.688 ± 0.349
1.879ArgMet: 1.879 ± 0.207
2.416ArgAsn: 2.416 ± 0.251
1.859ArgPro: 1.859 ± 0.196
1.879ArgGln: 1.879 ± 0.203
3.469ArgArg: 3.469 ± 0.342
2.87ArgSer: 2.87 ± 0.233
2.685ArgThr: 2.685 ± 0.262
3.552ArgVal: 3.552 ± 0.288
0.723ArgTrp: 0.723 ± 0.114
2.21ArgTyr: 2.21 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
4.192SerAla: 4.192 ± 0.287
0.578SerCys: 0.578 ± 0.123
3.635SerAsp: 3.635 ± 0.257
3.552SerGlu: 3.552 ± 0.248
2.334SerPhe: 2.334 ± 0.209
4.399SerGly: 4.399 ± 0.392
0.805SerHis: 0.805 ± 0.12
3.862SerIle: 3.862 ± 0.27
3.428SerLys: 3.428 ± 0.286
4.998SerLeu: 4.998 ± 0.309
1.714SerMet: 1.714 ± 0.18
3.49SerAsn: 3.49 ± 0.305
2.54SerPro: 2.54 ± 0.234
1.921SerGln: 1.921 ± 0.201
2.437SerArg: 2.437 ± 0.188
4.316SerSer: 4.316 ± 0.419
3.407SerThr: 3.407 ± 0.308
4.44SerVal: 4.44 ± 0.35
0.805SerTrp: 0.805 ± 0.137
2.437SerTyr: 2.437 ± 0.273
0.0SerXaa: 0.0 ± 0.0
Thr
4.729ThrAla: 4.729 ± 0.366
0.867ThrCys: 0.867 ± 0.129
3.738ThrAsp: 3.738 ± 0.327
4.068ThrGlu: 4.068 ± 0.297
2.54ThrPhe: 2.54 ± 0.23
4.729ThrGly: 4.729 ± 0.44
1.094ThrHis: 1.094 ± 0.178
4.006ThrIle: 4.006 ± 0.336
3.469ThrLys: 3.469 ± 0.241
5.101ThrLeu: 5.101 ± 0.354
1.115ThrMet: 1.115 ± 0.143
2.829ThrAsn: 2.829 ± 0.271
3.449ThrPro: 3.449 ± 0.282
1.797ThrGln: 1.797 ± 0.181
3.098ThrArg: 3.098 ± 0.282
3.118ThrSer: 3.118 ± 0.255
4.233ThrThr: 4.233 ± 0.445
4.977ThrVal: 4.977 ± 0.468
0.95ThrTrp: 0.95 ± 0.154
2.065ThrTyr: 2.065 ± 0.231
0.0ThrXaa: 0.0 ± 0.0
Val
5.225ValAla: 5.225 ± 0.344
0.888ValCys: 0.888 ± 0.138
5.059ValAsp: 5.059 ± 0.322
5.101ValGlu: 5.101 ± 0.35
2.953ValPhe: 2.953 ± 0.285
4.399ValGly: 4.399 ± 0.35
1.322ValHis: 1.322 ± 0.17
4.233ValIle: 4.233 ± 0.241
4.791ValLys: 4.791 ± 0.34
5.204ValLeu: 5.204 ± 0.328
2.106ValMet: 2.106 ± 0.18
3.903ValAsn: 3.903 ± 0.293
2.643ValPro: 2.643 ± 0.262
2.829ValGln: 2.829 ± 0.27
3.573ValArg: 3.573 ± 0.291
5.266ValSer: 5.266 ± 0.373
5.018ValThr: 5.018 ± 0.462
6.237ValVal: 6.237 ± 0.385
1.322ValTrp: 1.322 ± 0.17
2.747ValTyr: 2.747 ± 0.268
0.0ValXaa: 0.0 ± 0.0
Trp
1.136TrpAla: 1.136 ± 0.158
0.351TrpCys: 0.351 ± 0.101
0.991TrpAsp: 0.991 ± 0.173
1.136TrpGlu: 1.136 ± 0.143
0.764TrpPhe: 0.764 ± 0.118
0.805TrpGly: 0.805 ± 0.11
0.165TrpHis: 0.165 ± 0.064
0.743TrpIle: 0.743 ± 0.129
0.723TrpLys: 0.723 ± 0.141
1.528TrpLeu: 1.528 ± 0.171
0.434TrpMet: 0.434 ± 0.096
0.723TrpAsn: 0.723 ± 0.107
0.475TrpPro: 0.475 ± 0.088
0.372TrpGln: 0.372 ± 0.085
0.909TrpArg: 0.909 ± 0.108
0.743TrpSer: 0.743 ± 0.108
0.681TrpThr: 0.681 ± 0.119
0.971TrpVal: 0.971 ± 0.129
0.165TrpTrp: 0.165 ± 0.058
0.475TrpTyr: 0.475 ± 0.086
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.098TyrAla: 3.098 ± 0.257
0.496TyrCys: 0.496 ± 0.111
2.705TyrAsp: 2.705 ± 0.222
2.024TyrGlu: 2.024 ± 0.201
1.549TyrPhe: 1.549 ± 0.166
2.334TyrGly: 2.334 ± 0.241
1.033TyrHis: 1.033 ± 0.147
2.024TyrIle: 2.024 ± 0.216
1.879TyrLys: 1.879 ± 0.175
2.974TyrLeu: 2.974 ± 0.223
1.115TyrMet: 1.115 ± 0.155
2.106TyrAsn: 2.106 ± 0.198
1.508TyrPro: 1.508 ± 0.155
1.693TyrGln: 1.693 ± 0.23
1.962TyrArg: 1.962 ± 0.193
2.189TyrSer: 2.189 ± 0.192
2.292TyrThr: 2.292 ± 0.286
3.139TyrVal: 3.139 ± 0.265
0.434TyrTrp: 0.434 ± 0.093
1.156TyrTyr: 1.156 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 219 proteins (48425 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski