Aureococcus anophagefferens virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; unclassified Phycodnaviridae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 376 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076FGR0|A0A076FGR0_9PHYC Uncharacterized protein OS=Aureococcus anophagefferens virus OX=1474867 GN=AaV_341 PE=4 SV=1
MM1 pKa = 7.63CAMFHH6 pKa = 6.71CATSFNQDD14 pKa = 2.06ISVWDD19 pKa = 3.78VSNVTDD25 pKa = 3.51MKK27 pKa = 11.4GMFMCAKK34 pKa = 10.1SFNQPIGDD42 pKa = 3.49WDD44 pKa = 3.71VSNVFDD50 pKa = 3.97MSNMFEE56 pKa = 4.11NAEE59 pKa = 4.47SFNQDD64 pKa = 2.05IRR66 pKa = 11.84DD67 pKa = 3.55WDD69 pKa = 4.02TSKK72 pKa = 9.43VTYY75 pKa = 9.4MYY77 pKa = 11.6DD78 pKa = 3.1MFNDD82 pKa = 3.58AEE84 pKa = 4.44SFNLEE89 pKa = 3.86NAPWYY94 pKa = 10.57HH95 pKa = 7.53DD96 pKa = 3.78IDD98 pKa = 5.8DD99 pKa = 4.55DD100 pKa = 4.44

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076FG76|A0A076FG76_9PHYC Uncharacterized protein OS=Aureococcus anophagefferens virus OX=1474867 GN=AaV_243 PE=4 SV=1
MM1 pKa = 7.21YY2 pKa = 8.67LTYY5 pKa = 10.29IEE7 pKa = 4.89DD8 pKa = 3.59SRR10 pKa = 11.84KK11 pKa = 9.95KK12 pKa = 9.6SLEE15 pKa = 3.63RR16 pKa = 11.84VLEE19 pKa = 4.21VKK21 pKa = 10.4EE22 pKa = 4.01KK23 pKa = 9.75RR24 pKa = 11.84KK25 pKa = 10.29KK26 pKa = 8.09YY27 pKa = 10.72AKK29 pKa = 8.7GTSEE33 pKa = 3.93YY34 pKa = 10.78RR35 pKa = 11.84CLTKK39 pKa = 10.83LLALRR44 pKa = 11.84VKK46 pKa = 10.54SIRR49 pKa = 11.84QYY51 pKa = 11.59DD52 pKa = 3.65KK53 pKa = 10.2QLSRR57 pKa = 11.84FRR59 pKa = 4.35

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

376

0

376

107610

51

2073

286.2

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.439 ± 0.098

1.57 ± 0.07

6.833 ± 0.136

7.418 ± 0.16

5.697 ± 0.086

4.024 ± 0.144

1.674 ± 0.057

9.226 ± 0.164

10.383 ± 0.283

7.326 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.122

8.582 ± 0.157

2.937 ± 0.147

2.756 ± 0.054

3.065 ± 0.075

6.925 ± 0.148

5.181 ± 0.089

4.997 ± 0.114

1.067 ± 0.069

4.415 ± 0.08
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski