uncultured archaeon A07HN63

Taxonomy: cellular organisms; Archaea; environmental samples

Average proteome isoelectric point is 4.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4Y396|V4Y396_9ARCH Anthranilate synthase component 1 OS=uncultured archaeon A07HN63 OX=1412873 GN=trpE PE=3 SV=1
MM1 pKa = 7.4AADD4 pKa = 4.0FPEE7 pKa = 4.44YY8 pKa = 10.88LDD10 pKa = 3.61VDD12 pKa = 4.01YY13 pKa = 11.35TDD15 pKa = 5.72GEE17 pKa = 4.72GEE19 pKa = 4.59DD20 pKa = 3.63PADD23 pKa = 3.88YY24 pKa = 9.72PRR26 pKa = 11.84LPDD29 pKa = 4.53KK30 pKa = 10.72IEE32 pKa = 3.84KK33 pKa = 10.34AIEE36 pKa = 3.85VTQAGLEE43 pKa = 4.21QYY45 pKa = 9.18EE46 pKa = 4.55NPAVMWTGGKK56 pKa = 10.22DD57 pKa = 3.41STLTLYY63 pKa = 10.23FINQVAEE70 pKa = 4.14KK71 pKa = 10.28YY72 pKa = 10.81GYY74 pKa = 9.86DD75 pKa = 3.23KK76 pKa = 9.9PTAVFIDD83 pKa = 4.53HH84 pKa = 6.16YY85 pKa = 10.09QHH87 pKa = 6.87FDD89 pKa = 3.58EE90 pKa = 4.42ITDD93 pKa = 4.77FIEE96 pKa = 4.07HH97 pKa = 6.62WAEE100 pKa = 3.55EE101 pKa = 3.78WDD103 pKa = 3.12IDD105 pKa = 3.93IFYY108 pKa = 10.98ARR110 pKa = 11.84NEE112 pKa = 4.27DD113 pKa = 3.42VGEE116 pKa = 4.07YY117 pKa = 10.42ADD119 pKa = 3.77EE120 pKa = 5.21HH121 pKa = 7.38GLEE124 pKa = 4.7PGDD127 pKa = 5.14DD128 pKa = 3.62IPVSEE133 pKa = 4.48LSEE136 pKa = 4.47HH137 pKa = 5.61NQHH140 pKa = 6.25HH141 pKa = 5.65VRR143 pKa = 11.84EE144 pKa = 4.12ILEE147 pKa = 4.28YY148 pKa = 10.79EE149 pKa = 4.55DD150 pKa = 3.76DD151 pKa = 4.0TFPFLLDD158 pKa = 3.58TYY160 pKa = 11.08VGNHH164 pKa = 5.81LLKK167 pKa = 10.04TVALNDD173 pKa = 3.69ALEE176 pKa = 4.15SHH178 pKa = 7.5DD179 pKa = 3.7IDD181 pKa = 4.14GVISGVRR188 pKa = 11.84WDD190 pKa = 3.82EE191 pKa = 3.83QEE193 pKa = 3.94ARR195 pKa = 11.84ADD197 pKa = 3.49EE198 pKa = 4.57TFFSARR204 pKa = 11.84HH205 pKa = 6.25DD206 pKa = 3.76PDD208 pKa = 3.7IYY210 pKa = 10.37PPHH213 pKa = 7.33DD214 pKa = 5.14RR215 pKa = 11.84IQPILQFDD223 pKa = 4.08EE224 pKa = 4.59SDD226 pKa = 3.31VWDD229 pKa = 3.89VFWNYY234 pKa = 8.94VVPDD238 pKa = 3.85TVEE241 pKa = 4.1AFPEE245 pKa = 4.27DD246 pKa = 4.33GYY248 pKa = 11.71VPEE251 pKa = 4.97SADD254 pKa = 3.71DD255 pKa = 3.84LPEE258 pKa = 4.37GVEE261 pKa = 3.94QDD263 pKa = 4.49DD264 pKa = 4.66VPISPP269 pKa = 4.64

Molecular weight: 30.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5A2G0|V5A2G0_9ARCH Uncharacterized protein OS=uncultured archaeon A07HN63 OX=1412873 GN=A07HN63_00410 PE=4 SV=1
MM1 pKa = 7.81SSPTPVASVAPPHH14 pKa = 6.27PRR16 pKa = 11.84VPRR19 pKa = 11.84PVSTRR24 pKa = 11.84LSNSHH29 pKa = 6.03RR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 9.82PSPAPASTRR41 pKa = 11.84CRR43 pKa = 11.84GWSAPSTPAISAPSTRR59 pKa = 11.84RR60 pKa = 11.84CGRR63 pKa = 11.84PTAPTTSPRR72 pKa = 11.84SGPTARR78 pKa = 11.84SPSRR82 pKa = 11.84WPPPRR87 pKa = 11.84PRR89 pKa = 11.84RR90 pKa = 11.84HH91 pKa = 6.34AGCATLPASRR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84VPRR106 pKa = 11.84RR107 pKa = 11.84QLPDD111 pKa = 3.31PARR114 pKa = 11.84QRR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84WRR120 pKa = 11.84RR121 pKa = 11.84TRR123 pKa = 11.84QGRR126 pKa = 11.84DD127 pKa = 2.63RR128 pKa = 11.84HH129 pKa = 6.32SGVPRR134 pKa = 11.84RR135 pKa = 11.84AGWRR139 pKa = 11.84AEE141 pKa = 4.0CLRR144 pKa = 11.84GRR146 pKa = 11.84LRR148 pKa = 11.84QRR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84PRR154 pKa = 11.84HH155 pKa = 4.77DD156 pKa = 2.95RR157 pKa = 11.84RR158 pKa = 11.84YY159 pKa = 10.21LRR161 pKa = 11.84RR162 pKa = 11.84ARR164 pKa = 11.84YY165 pKa = 9.07RR166 pKa = 11.84RR167 pKa = 11.84RR168 pKa = 11.84QGRR171 pKa = 11.84RR172 pKa = 11.84GRR174 pKa = 11.84VGASDD179 pKa = 3.26RR180 pKa = 11.84RR181 pKa = 3.78

Molecular weight: 20.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2505

0

2505

612759

28

1746

244.6

26.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.098 ± 0.06

0.754 ± 0.018

8.925 ± 0.07

8.219 ± 0.069

3.085 ± 0.03

8.205 ± 0.047

1.965 ± 0.024

4.644 ± 0.037

1.851 ± 0.028

8.741 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.741 ± 0.022

2.334 ± 0.025

4.583 ± 0.03

2.883 ± 0.029

6.152 ± 0.046

5.783 ± 0.042

6.948 ± 0.047

8.455 ± 0.052

1.04 ± 0.021

2.49 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski