Bosea sp. OK403

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6030 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3L178|A0A1I3L178_9BRAD Uncharacterized protein OS=Bosea sp. OK403 OX=1855286 GN=SAMN05216304_103499 PE=4 SV=1
MM1 pKa = 7.58SFTLTPDD8 pKa = 3.43NAPLADD14 pKa = 3.73VAGLNAPIGSTILKK28 pKa = 10.17HH29 pKa = 6.3LDD31 pKa = 2.56QWYY34 pKa = 10.33YY35 pKa = 11.46LDD37 pKa = 5.24DD38 pKa = 5.07SIGAIAA44 pKa = 4.94

Molecular weight: 4.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3R4M1|A0A1I3R4M1_9BRAD Chitooligosaccharide deacetylase OS=Bosea sp. OK403 OX=1855286 GN=SAMN05216304_107212 PE=3 SV=1
MM1 pKa = 7.07GVIVAVVIMAASGMVVRR18 pKa = 11.84VLFDD22 pKa = 3.56RR23 pKa = 11.84FASRR27 pKa = 11.84RR28 pKa = 11.84SMVMAAATLIRR39 pKa = 11.84GSLHH43 pKa = 5.84GRR45 pKa = 11.84IAMSGVVMSVVIMPASRR62 pKa = 11.84VVVRR66 pKa = 11.84MLFGGLWSRR75 pKa = 11.84RR76 pKa = 11.84GMVMAAATLIHH87 pKa = 6.53RR88 pKa = 11.84CLRR91 pKa = 11.84GRR93 pKa = 11.84VAKK96 pKa = 10.53NVVIMSLVVMSAVVMVTRR114 pKa = 5.02

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6030

0

6030

1935374

39

6419

321.0

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.431 ± 0.046

0.811 ± 0.009

5.215 ± 0.023

5.366 ± 0.029

3.712 ± 0.022

8.764 ± 0.039

1.902 ± 0.017

5.281 ± 0.023

3.155 ± 0.024

10.651 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.015

2.36 ± 0.021

5.342 ± 0.026

3.099 ± 0.016

7.097 ± 0.032

5.461 ± 0.029

5.248 ± 0.034

7.348 ± 0.026

1.297 ± 0.014

2.079 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski