Proteome-pI 2.0 - Proteome Isoelectric Point Database

Thanatephorus cucumeris (strain AG1-IA) (Rice sheath blight fungus) (Rhizoctonia solani)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Cantharellales; Ceratobasidiaceae; Rhizoctonia; Rhizoctonia solani; Rhizoctonia solani AG-1

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 10482 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|L8WMV8|L8WMV8_THACA APH domain-containing protein OS=Thanatephorus cucumeris (strain AG1-IA) OX=983506 GN=AG1IA_07856 PE=4 SV=1

MM1 pKa = 8.15DD2 pKa = 5.93DD3 pKa = 3.76KK4 pKa = 11.49SVAAGPFDD12 pKa = 4.6SIPRR16 pKa = 11.84VWQFSCDD23 pKa = 3.59STCVIVGFEE32 pKa = 4.24DD33 pKa = 3.84GRR35 pKa = 11.84IEE37 pKa = 4.88GICLEE42 pKa = 4.3TGQTAFEE49 pKa = 4.63LRR51 pKa = 11.84SADD54 pKa = 4.26DD55 pKa = 3.68DD56 pKa = 4.03WVDD59 pKa = 6.22LIAEE63 pKa = 4.49SPDD66 pKa = 3.41CSLLASIDD74 pKa = 3.89DD75 pKa = 4.32TEE77 pKa = 5.71HH78 pKa = 7.42DD79 pKa = 4.34SPSLRR84 pKa = 11.84IWSMAGPTLCFQTSDD99 pKa = 4.95DD100 pKa = 4.68LPLDD104 pKa = 4.36PGPGQTLSGLYY115 pKa = 9.14DD116 pKa = 3.22QCHH119 pKa = 6.48IDD121 pKa = 4.38SDD123 pKa = 4.28GCMVNSNNDD132 pKa = 3.57LLIWIPAEE140 pKa = 3.77IADD143 pKa = 4.34AGLSPFVFVIVTEE156 pKa = 4.27SGTLQVPKK164 pKa = 10.74QMLIAGDD171 pKa = 3.69QWHH174 pKa = 5.65QCYY177 pKa = 10.47SRR179 pKa = 11.84LSFHH183 pKa = 6.75AHH185 pKa = 5.75

Molecular weight: 20.2 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|L8WLR3|L8WLR3_THACA Uncharacterized protein OS=Thanatephorus cucumeris (strain AG1-IA) OX=983506 GN=AG1IA_06860 PE=4 SV=1

MM1 pKa = 7.49GAPAFLAPKK10 pKa = 9.05PPLVVRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 10.05KK20 pKa = 8.9MNGARR25 pKa = 11.84RR26 pKa = 11.84MM27 pKa = 3.65

Molecular weight: 3.05 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
10482	0	10482	4287830	20	4709	409.1	45.12

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.266 ± 0.022	1.498 ± 0.01	5.099 ± 0.014	5.487 ± 0.02	3.417 ± 0.013	6.917 ± 0.022	2.618 ± 0.011	4.975 ± 0.015	4.288 ± 0.022	8.937 ± 0.025

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.114 ± 0.009	3.514 ± 0.012	6.78 ± 0.03	3.723 ± 0.014	6.699 ± 0.02	8.939 ± 0.032	6.247 ± 0.017	6.333 ± 0.016	1.453 ± 0.009	2.696 ± 0.01

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski