Amino acid dipepetide frequency for Pseudomonas phage JBD69

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.384AlaAla: 18.384 ± 2.717
1.518AlaCys: 1.518 ± 0.416
7.59AlaAsp: 7.59 ± 0.74
8.939AlaGlu: 8.939 ± 1.286
2.699AlaPhe: 2.699 ± 0.446
8.939AlaGly: 8.939 ± 1.132
1.518AlaHis: 1.518 ± 0.283
5.903AlaIle: 5.903 ± 0.641
4.132AlaLys: 4.132 ± 0.712
13.409AlaLeu: 13.409 ± 1.238
3.373AlaMet: 3.373 ± 0.441
3.289AlaAsn: 3.289 ± 0.631
5.566AlaPro: 5.566 ± 0.654
5.566AlaGln: 5.566 ± 0.861
10.373AlaArg: 10.373 ± 1.189
6.915AlaSer: 6.915 ± 0.906
6.578AlaThr: 6.578 ± 0.582
6.409AlaVal: 6.409 ± 0.743
2.614AlaTrp: 2.614 ± 0.307
2.53AlaTyr: 2.53 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
0.843CysAla: 0.843 ± 0.246
0.084CysCys: 0.084 ± 0.097
0.928CysAsp: 0.928 ± 0.302
0.422CysGlu: 0.422 ± 0.256
0.506CysPhe: 0.506 ± 0.214
0.422CysGly: 0.422 ± 0.207
0.422CysHis: 0.422 ± 0.219
0.253CysIle: 0.253 ± 0.145
0.169CysLys: 0.169 ± 0.11
0.422CysLeu: 0.422 ± 0.189
0.253CysMet: 0.253 ± 0.189
0.506CysAsn: 0.506 ± 0.265
0.59CysPro: 0.59 ± 0.237
0.0CysGln: 0.0 ± 0.0
1.096CysArg: 1.096 ± 0.311
0.506CysSer: 0.506 ± 0.182
0.59CysThr: 0.59 ± 0.216
0.253CysVal: 0.253 ± 0.148
0.422CysTrp: 0.422 ± 0.185
0.253CysTyr: 0.253 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
6.747AspAla: 6.747 ± 0.912
0.253AspCys: 0.253 ± 0.139
3.289AspAsp: 3.289 ± 0.588
3.373AspGlu: 3.373 ± 0.568
1.687AspPhe: 1.687 ± 0.276
6.662AspGly: 6.662 ± 0.854
1.181AspHis: 1.181 ± 0.359
2.361AspIle: 2.361 ± 0.338
1.518AspLys: 1.518 ± 0.337
5.313AspLeu: 5.313 ± 0.592
1.518AspMet: 1.518 ± 0.444
1.518AspAsn: 1.518 ± 0.292
3.205AspPro: 3.205 ± 0.629
2.867AspGln: 2.867 ± 0.521
3.12AspArg: 3.12 ± 0.522
3.458AspSer: 3.458 ± 0.543
3.879AspThr: 3.879 ± 0.513
3.373AspVal: 3.373 ± 0.561
1.181AspTrp: 1.181 ± 0.249
1.096AspTyr: 1.096 ± 0.267
0.0AspXaa: 0.0 ± 0.0
Glu
7.084GluAla: 7.084 ± 0.789
0.506GluCys: 0.506 ± 0.247
2.614GluAsp: 2.614 ± 0.491
2.699GluGlu: 2.699 ± 0.409
2.108GluPhe: 2.108 ± 0.392
3.12GluGly: 3.12 ± 0.504
0.843GluHis: 0.843 ± 0.204
3.12GluIle: 3.12 ± 0.449
2.53GluLys: 2.53 ± 0.485
6.662GluLeu: 6.662 ± 0.698
1.94GluMet: 1.94 ± 0.407
2.108GluAsn: 2.108 ± 0.619
2.867GluPro: 2.867 ± 0.481
4.048GluGln: 4.048 ± 0.575
4.638GluArg: 4.638 ± 0.796
3.036GluSer: 3.036 ± 0.443
3.205GluThr: 3.205 ± 0.451
4.807GluVal: 4.807 ± 0.556
1.181GluTrp: 1.181 ± 0.289
1.771GluTyr: 1.771 ± 0.357
0.0GluXaa: 0.0 ± 0.0
Phe
3.458PheAla: 3.458 ± 0.477
0.506PheCys: 0.506 ± 0.197
2.53PheAsp: 2.53 ± 0.512
1.349PheGlu: 1.349 ± 0.406
0.759PhePhe: 0.759 ± 0.267
2.361PheGly: 2.361 ± 0.372
0.506PheHis: 0.506 ± 0.202
0.928PheIle: 0.928 ± 0.221
0.675PheLys: 0.675 ± 0.218
2.361PheLeu: 2.361 ± 0.38
0.928PheMet: 0.928 ± 0.243
1.096PheAsn: 1.096 ± 0.305
1.349PhePro: 1.349 ± 0.34
1.434PheGln: 1.434 ± 0.301
2.024PheArg: 2.024 ± 0.518
1.181PheSer: 1.181 ± 0.305
1.434PheThr: 1.434 ± 0.357
1.265PheVal: 1.265 ± 0.264
0.337PheTrp: 0.337 ± 0.192
1.012PheTyr: 1.012 ± 0.282
0.0PheXaa: 0.0 ± 0.0
Gly
7.168GlyAla: 7.168 ± 0.804
0.59GlyCys: 0.59 ± 0.238
4.048GlyAsp: 4.048 ± 0.791
4.217GlyGlu: 4.217 ± 0.485
2.699GlyPhe: 2.699 ± 0.433
6.241GlyGly: 6.241 ± 0.623
1.012GlyHis: 1.012 ± 0.245
4.048GlyIle: 4.048 ± 0.547
3.205GlyLys: 3.205 ± 0.558
8.264GlyLeu: 8.264 ± 0.924
1.349GlyMet: 1.349 ± 0.391
2.53GlyAsn: 2.53 ± 0.414
2.783GlyPro: 2.783 ± 0.432
5.06GlyGln: 5.06 ± 0.559
6.072GlyArg: 6.072 ± 0.619
4.554GlySer: 4.554 ± 0.52
3.626GlyThr: 3.626 ± 0.474
4.807GlyVal: 4.807 ± 0.675
1.518GlyTrp: 1.518 ± 0.326
2.193GlyTyr: 2.193 ± 0.568
0.0GlyXaa: 0.0 ± 0.0
His
2.024HisAla: 2.024 ± 0.323
0.084HisCys: 0.084 ± 0.089
1.012HisAsp: 1.012 ± 0.266
1.012HisGlu: 1.012 ± 0.315
0.506HisPhe: 0.506 ± 0.209
1.181HisGly: 1.181 ± 0.346
0.169HisHis: 0.169 ± 0.094
0.675HisIle: 0.675 ± 0.221
0.169HisLys: 0.169 ± 0.111
1.687HisLeu: 1.687 ± 0.377
0.928HisMet: 0.928 ± 0.297
0.843HisAsn: 0.843 ± 0.256
1.265HisPro: 1.265 ± 0.366
1.181HisGln: 1.181 ± 0.378
1.349HisArg: 1.349 ± 0.326
0.506HisSer: 0.506 ± 0.221
0.843HisThr: 0.843 ± 0.231
0.843HisVal: 0.843 ± 0.263
0.169HisTrp: 0.169 ± 0.115
0.843HisTyr: 0.843 ± 0.296
0.0HisXaa: 0.0 ± 0.0
Ile
4.976IleAla: 4.976 ± 0.897
0.506IleCys: 0.506 ± 0.218
3.12IleAsp: 3.12 ± 0.435
3.373IleGlu: 3.373 ± 0.507
0.843IlePhe: 0.843 ± 0.218
3.289IleGly: 3.289 ± 0.52
1.096IleHis: 1.096 ± 0.267
1.855IleIle: 1.855 ± 0.513
1.855IleLys: 1.855 ± 0.433
2.783IleLeu: 2.783 ± 0.455
0.675IleMet: 0.675 ± 0.241
1.096IleAsn: 1.096 ± 0.377
2.614IlePro: 2.614 ± 0.457
1.687IleGln: 1.687 ± 0.479
4.048IleArg: 4.048 ± 0.53
2.108IleSer: 2.108 ± 0.431
3.289IleThr: 3.289 ± 0.452
2.446IleVal: 2.446 ± 0.391
0.675IleTrp: 0.675 ± 0.273
1.096IleTyr: 1.096 ± 0.271
0.0IleXaa: 0.0 ± 0.0
Lys
4.891LysAla: 4.891 ± 0.701
0.169LysCys: 0.169 ± 0.116
1.012LysAsp: 1.012 ± 0.377
1.771LysGlu: 1.771 ± 0.45
0.506LysPhe: 0.506 ± 0.18
2.53LysGly: 2.53 ± 0.282
0.59LysHis: 0.59 ± 0.203
1.181LysIle: 1.181 ± 0.307
1.434LysLys: 1.434 ± 0.298
2.952LysLeu: 2.952 ± 0.516
0.422LysMet: 0.422 ± 0.18
1.096LysAsn: 1.096 ± 0.241
2.277LysPro: 2.277 ± 0.535
1.096LysGln: 1.096 ± 0.251
3.205LysArg: 3.205 ± 0.658
2.446LysSer: 2.446 ± 0.539
2.193LysThr: 2.193 ± 0.413
2.614LysVal: 2.614 ± 0.426
0.169LysTrp: 0.169 ± 0.104
0.759LysTyr: 0.759 ± 0.197
0.0LysXaa: 0.0 ± 0.0
Leu
14.758LeuAla: 14.758 ± 1.414
0.928LeuCys: 0.928 ± 0.23
6.072LeuAsp: 6.072 ± 0.65
6.915LeuGlu: 6.915 ± 0.8
2.193LeuPhe: 2.193 ± 0.582
7.421LeuGly: 7.421 ± 0.619
2.53LeuHis: 2.53 ± 0.474
3.036LeuIle: 3.036 ± 0.559
3.458LeuLys: 3.458 ± 0.634
9.023LeuLeu: 9.023 ± 0.893
1.771LeuMet: 1.771 ± 0.439
2.867LeuAsn: 2.867 ± 0.462
4.723LeuPro: 4.723 ± 0.694
3.879LeuGln: 3.879 ± 0.545
7.421LeuArg: 7.421 ± 0.775
4.891LeuSer: 4.891 ± 0.712
5.313LeuThr: 5.313 ± 0.763
7.674LeuVal: 7.674 ± 0.691
1.349LeuTrp: 1.349 ± 0.34
2.277LeuTyr: 2.277 ± 0.449
0.0LeuXaa: 0.0 ± 0.0
Met
3.795MetAla: 3.795 ± 0.539
0.084MetCys: 0.084 ± 0.094
2.361MetAsp: 2.361 ± 0.44
1.602MetGlu: 1.602 ± 0.435
0.422MetPhe: 0.422 ± 0.186
1.771MetGly: 1.771 ± 0.314
0.169MetHis: 0.169 ± 0.113
0.422MetIle: 0.422 ± 0.198
0.675MetLys: 0.675 ± 0.244
1.265MetLeu: 1.265 ± 0.314
0.422MetMet: 0.422 ± 0.187
0.59MetAsn: 0.59 ± 0.258
1.349MetPro: 1.349 ± 0.448
1.181MetGln: 1.181 ± 0.303
1.771MetArg: 1.771 ± 0.352
1.771MetSer: 1.771 ± 0.381
1.265MetThr: 1.265 ± 0.281
0.928MetVal: 0.928 ± 0.335
0.253MetTrp: 0.253 ± 0.176
0.253MetTyr: 0.253 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
3.289AsnAla: 3.289 ± 0.587
0.084AsnCys: 0.084 ± 0.076
1.265AsnAsp: 1.265 ± 0.273
1.265AsnGlu: 1.265 ± 0.297
0.675AsnPhe: 0.675 ± 0.327
3.205AsnGly: 3.205 ± 0.711
0.59AsnHis: 0.59 ± 0.235
1.096AsnIle: 1.096 ± 0.345
0.759AsnLys: 0.759 ± 0.268
3.289AsnLeu: 3.289 ± 0.525
0.675AsnMet: 0.675 ± 0.212
1.096AsnAsn: 1.096 ± 0.34
2.53AsnPro: 2.53 ± 0.549
1.096AsnGln: 1.096 ± 0.279
2.614AsnArg: 2.614 ± 0.37
2.108AsnSer: 2.108 ± 0.463
1.265AsnThr: 1.265 ± 0.321
1.181AsnVal: 1.181 ± 0.262
0.506AsnTrp: 0.506 ± 0.173
0.928AsnTyr: 0.928 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
7.168ProAla: 7.168 ± 0.835
0.506ProCys: 0.506 ± 0.227
3.964ProAsp: 3.964 ± 0.675
2.699ProGlu: 2.699 ± 0.457
1.771ProPhe: 1.771 ± 0.378
4.048ProGly: 4.048 ± 0.535
0.843ProHis: 0.843 ± 0.475
2.024ProIle: 2.024 ± 0.404
1.855ProLys: 1.855 ± 0.366
4.976ProLeu: 4.976 ± 0.602
0.759ProMet: 0.759 ± 0.264
1.771ProAsn: 1.771 ± 0.343
2.193ProPro: 2.193 ± 0.475
2.108ProGln: 2.108 ± 0.475
3.036ProArg: 3.036 ± 0.464
3.373ProSer: 3.373 ± 0.411
3.12ProThr: 3.12 ± 0.521
3.12ProVal: 3.12 ± 0.639
0.337ProTrp: 0.337 ± 0.134
1.265ProTyr: 1.265 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
5.903GlnAla: 5.903 ± 1.064
0.253GlnCys: 0.253 ± 0.148
1.855GlnAsp: 1.855 ± 0.346
1.771GlnGlu: 1.771 ± 0.381
1.687GlnPhe: 1.687 ± 0.316
3.373GlnGly: 3.373 ± 0.382
0.928GlnHis: 0.928 ± 0.262
2.277GlnIle: 2.277 ± 0.274
1.012GlnLys: 1.012 ± 0.285
6.325GlnLeu: 6.325 ± 0.616
1.349GlnMet: 1.349 ± 0.392
1.265GlnAsn: 1.265 ± 0.288
2.952GlnPro: 2.952 ± 0.414
2.867GlnGln: 2.867 ± 0.648
3.879GlnArg: 3.879 ± 0.467
2.783GlnSer: 2.783 ± 0.433
2.193GlnThr: 2.193 ± 0.39
4.554GlnVal: 4.554 ± 0.637
0.759GlnTrp: 0.759 ± 0.238
0.59GlnTyr: 0.59 ± 0.24
0.0GlnXaa: 0.0 ± 0.0
Arg
8.18ArgAla: 8.18 ± 0.901
0.759ArgCys: 0.759 ± 0.274
3.879ArgAsp: 3.879 ± 0.565
5.65ArgGlu: 5.65 ± 0.704
2.193ArgPhe: 2.193 ± 0.469
4.301ArgGly: 4.301 ± 0.551
1.855ArgHis: 1.855 ± 0.376
4.217ArgIle: 4.217 ± 0.528
2.193ArgLys: 2.193 ± 0.388
8.264ArgLeu: 8.264 ± 0.866
1.265ArgMet: 1.265 ± 0.3
1.434ArgAsn: 1.434 ± 0.376
4.048ArgPro: 4.048 ± 0.874
5.06ArgGln: 5.06 ± 0.723
5.735ArgArg: 5.735 ± 0.739
3.964ArgSer: 3.964 ± 0.626
3.879ArgThr: 3.879 ± 0.584
4.132ArgVal: 4.132 ± 0.628
1.518ArgTrp: 1.518 ± 0.423
3.12ArgTyr: 3.12 ± 0.493
0.0ArgXaa: 0.0 ± 0.0
Ser
7.505SerAla: 7.505 ± 0.786
0.759SerCys: 0.759 ± 0.244
3.289SerAsp: 3.289 ± 0.652
3.458SerGlu: 3.458 ± 0.43
1.771SerPhe: 1.771 ± 0.245
4.47SerGly: 4.47 ± 0.672
0.759SerHis: 0.759 ± 0.235
2.952SerIle: 2.952 ± 0.476
2.024SerLys: 2.024 ± 0.455
5.735SerLeu: 5.735 ± 0.8
0.675SerMet: 0.675 ± 0.234
1.349SerAsn: 1.349 ± 0.358
2.952SerPro: 2.952 ± 0.478
2.193SerGln: 2.193 ± 0.381
3.626SerArg: 3.626 ± 0.651
4.554SerSer: 4.554 ± 0.838
3.879SerThr: 3.879 ± 0.721
3.373SerVal: 3.373 ± 0.537
1.687SerTrp: 1.687 ± 0.353
1.349SerTyr: 1.349 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
7.843ThrAla: 7.843 ± 0.983
0.337ThrCys: 0.337 ± 0.243
3.626ThrAsp: 3.626 ± 0.47
2.699ThrGlu: 2.699 ± 0.494
1.265ThrPhe: 1.265 ± 0.346
5.144ThrGly: 5.144 ± 0.765
0.59ThrHis: 0.59 ± 0.239
1.94ThrIle: 1.94 ± 0.387
2.108ThrLys: 2.108 ± 0.444
5.903ThrLeu: 5.903 ± 0.617
1.265ThrMet: 1.265 ± 0.255
1.434ThrAsn: 1.434 ± 0.272
1.855ThrPro: 1.855 ± 0.328
1.434ThrGln: 1.434 ± 0.264
3.205ThrArg: 3.205 ± 0.385
3.795ThrSer: 3.795 ± 0.723
3.795ThrThr: 3.795 ± 0.606
5.482ThrVal: 5.482 ± 0.832
0.843ThrTrp: 0.843 ± 0.256
1.602ThrTyr: 1.602 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
7.59ValAla: 7.59 ± 0.828
0.422ValCys: 0.422 ± 0.175
3.12ValAsp: 3.12 ± 0.444
5.144ValGlu: 5.144 ± 0.734
1.602ValPhe: 1.602 ± 0.349
4.132ValGly: 4.132 ± 0.609
0.843ValHis: 0.843 ± 0.291
2.952ValIle: 2.952 ± 0.434
2.108ValLys: 2.108 ± 0.436
5.903ValLeu: 5.903 ± 0.634
1.349ValMet: 1.349 ± 0.359
2.277ValAsn: 2.277 ± 0.366
3.036ValPro: 3.036 ± 0.443
3.542ValGln: 3.542 ± 0.584
4.891ValArg: 4.891 ± 0.507
3.711ValSer: 3.711 ± 0.458
3.964ValThr: 3.964 ± 0.612
4.217ValVal: 4.217 ± 0.672
1.012ValTrp: 1.012 ± 0.301
2.867ValTyr: 2.867 ± 0.583
0.0ValXaa: 0.0 ± 0.0
Trp
1.602TrpAla: 1.602 ± 0.306
0.337TrpCys: 0.337 ± 0.146
0.422TrpAsp: 0.422 ± 0.198
0.928TrpGlu: 0.928 ± 0.277
0.59TrpPhe: 0.59 ± 0.217
0.928TrpGly: 0.928 ± 0.297
0.169TrpHis: 0.169 ± 0.111
1.181TrpIle: 1.181 ± 0.283
0.759TrpLys: 0.759 ± 0.191
1.771TrpLeu: 1.771 ± 0.351
0.843TrpMet: 0.843 ± 0.303
0.422TrpAsn: 0.422 ± 0.155
0.928TrpPro: 0.928 ± 0.313
0.843TrpGln: 0.843 ± 0.179
1.434TrpArg: 1.434 ± 0.335
1.181TrpSer: 1.181 ± 0.293
0.675TrpThr: 0.675 ± 0.245
1.349TrpVal: 1.349 ± 0.337
0.422TrpTrp: 0.422 ± 0.181
0.506TrpTyr: 0.506 ± 0.258
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.036TyrAla: 3.036 ± 0.412
0.337TyrCys: 0.337 ± 0.173
1.602TyrAsp: 1.602 ± 0.322
1.602TyrGlu: 1.602 ± 0.413
1.012TyrPhe: 1.012 ± 0.268
2.446TyrGly: 2.446 ± 0.449
0.675TyrHis: 0.675 ± 0.344
1.012TyrIle: 1.012 ± 0.294
0.675TyrLys: 0.675 ± 0.273
2.193TyrLeu: 2.193 ± 0.488
0.506TyrMet: 0.506 ± 0.207
0.928TyrAsn: 0.928 ± 0.224
1.855TyrPro: 1.855 ± 0.456
1.434TyrGln: 1.434 ± 0.323
2.108TyrArg: 2.108 ± 0.365
1.518TyrSer: 1.518 ± 0.36
1.181TyrThr: 1.181 ± 0.321
1.855TyrVal: 1.855 ± 0.358
0.337TyrTrp: 0.337 ± 0.156
0.506TyrTyr: 0.506 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (11859 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski