Weissella phage WCP30

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1SDW6|A0A1B1SDW6_9CAUD Putative holin OS=Weissella phage WCP30 OX=1837862 PE=4 SV=1
MM1 pKa = 7.92SMTFDD6 pKa = 3.06EE7 pKa = 5.37AMAKK11 pKa = 10.11VGTYY15 pKa = 9.69LQNNDD20 pKa = 4.56LSNPDD25 pKa = 4.19DD26 pKa = 3.76NDD28 pKa = 3.2KK29 pKa = 10.99FFYY32 pKa = 10.46IILNGLQNQYY42 pKa = 11.34APTIEE47 pKa = 4.21MTRR50 pKa = 11.84TQYY53 pKa = 11.45NFLVGSDD60 pKa = 3.87GLVDD64 pKa = 4.28IIGNITVDD72 pKa = 4.55GIVKK76 pKa = 10.09EE77 pKa = 4.16YY78 pKa = 10.57FDD80 pKa = 5.22DD81 pKa = 4.94GFWYY85 pKa = 9.61PLNEE89 pKa = 3.82RR90 pKa = 11.84DD91 pKa = 3.49VANAFLHH98 pKa = 6.36PEE100 pKa = 3.97TVKK103 pKa = 10.59VVKK106 pKa = 10.56

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1SE42|A0A1B1SE42_9CAUD Head morphogenesis protein OS=Weissella phage WCP30 OX=1837862 PE=4 SV=1
MM1 pKa = 7.53ANIIGADD8 pKa = 3.38KK9 pKa = 10.66FIAAVSRR16 pKa = 11.84KK17 pKa = 9.96ANAAPKK23 pKa = 10.05KK24 pKa = 8.7VANVLRR30 pKa = 11.84SEE32 pKa = 4.21TAAMQSQAQRR42 pKa = 11.84LEE44 pKa = 3.87PVRR47 pKa = 11.84TGNLRR52 pKa = 11.84RR53 pKa = 11.84STNISVTTSGNTAIGTVTANAYY75 pKa = 8.85NKK77 pKa = 10.18SYY79 pKa = 11.08NYY81 pKa = 10.72GYY83 pKa = 10.54AQEE86 pKa = 4.34NGTRR90 pKa = 11.84YY91 pKa = 9.65IRR93 pKa = 11.84GKK95 pKa = 10.17HH96 pKa = 4.69FMEE99 pKa = 4.61TAFNAHH105 pKa = 5.53SQSFKK110 pKa = 10.85QKK112 pKa = 8.93MSEE115 pKa = 4.07VLKK118 pKa = 11.08

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

8860

100

1028

253.1

28.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.499 ± 0.726

0.282 ± 0.136

6.061 ± 0.391

5.914 ± 0.691

4.345 ± 0.256

7.359 ± 0.51

1.287 ± 0.158

6.027 ± 0.377

6.637 ± 0.369

7.144 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.219

6.772 ± 0.426

2.754 ± 0.198

3.95 ± 0.202

3.747 ± 0.404

6.603 ± 0.544

6.806 ± 0.434

7.009 ± 0.326

1.84 ± 0.32

4.21 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski