Tortoise microvirus 104

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W6Z6|A0A4P8W6Z6_9VIRU Uncharacterized protein OS=Tortoise microvirus 104 OX=2583104 PE=4 SV=1
MM1 pKa = 7.65HH2 pKa = 7.03NNIYY6 pKa = 10.22IIYY9 pKa = 8.43NVLSKK14 pKa = 10.76RR15 pKa = 11.84YY16 pKa = 8.99GDD18 pKa = 3.6VVAYY22 pKa = 9.65PSDD25 pKa = 3.22AFAARR30 pKa = 11.84RR31 pKa = 11.84LSEE34 pKa = 4.12VYY36 pKa = 9.97KK37 pKa = 10.48DD38 pKa = 3.69HH39 pKa = 7.66PEE41 pKa = 3.89HH42 pKa = 6.2LKK44 pKa = 10.86EE45 pKa = 4.45MEE47 pKa = 4.02LCRR50 pKa = 11.84IGSVSIEE57 pKa = 4.19TGVVTPHH64 pKa = 6.61DD65 pKa = 5.19PIRR68 pKa = 11.84IDD70 pKa = 4.65LDD72 pKa = 3.55VDD74 pKa = 3.64PDD76 pKa = 4.01SPLSLNEE83 pKa = 3.74

Molecular weight:
9.44 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4V1FW28|A0A4V1FW28_9VIRU Uncharacterized protein OS=Tortoise microvirus 104 OX=2583104 PE=4 SV=1
MM1 pKa = 7.64GNLCSIRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 10.35LSITRR15 pKa = 11.84QHH17 pKa = 6.17TPQSINYY24 pKa = 7.6QLQYY28 pKa = 10.95SEE30 pKa = 4.72TVSHH34 pKa = 6.63KK35 pKa = 10.3HH36 pKa = 5.69PKK38 pKa = 9.01LAFWVQLPVLPCRR51 pKa = 11.84AKK53 pKa = 9.84RR54 pKa = 11.84EE55 pKa = 4.24KK56 pKa = 10.92KK57 pKa = 10.14NFHH60 pKa = 5.8LTHH63 pKa = 5.77VKK65 pKa = 10.23KK66 pKa = 10.94SNKK69 pKa = 9.67NYY71 pKa = 8.51PQSNKK76 pKa = 10.09KK77 pKa = 8.98MLTTFFICVILILGGWHH94 pKa = 7.3DD95 pKa = 5.05LYY97 pKa = 11.51NAA99 pKa = 4.33

Molecular weight:
11.66 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1720

67

572

191.1

21.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.57 ± 0.94

1.279 ± 0.413

6.163 ± 0.615

4.826 ± 0.944

3.779 ± 0.782

4.942 ± 0.532

2.093 ± 0.454

6.395 ± 0.585

6.744 ± 1.464

8.779 ± 0.469

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.365

5.174 ± 0.394

4.244 ± 0.748

6.279 ± 1.396

4.709 ± 0.232

7.733 ± 1.002

6.744 ± 0.748

4.36 ± 0.464

0.872 ± 0.148

5.64 ± 0.621

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski