Aquamicrobium sp. LC103

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Aquamicrobium; unclassified Aquamicrobium

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V6BEV5|A0A4V6BEV5_9RHIZ Coenzyme F420-0:L-glutamate ligase OS=Aquamicrobium sp. LC103 OX=1120658 GN=cofE PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84IIFSTAVFLAFALPLQAAEE21 pKa = 4.14ITIDD25 pKa = 3.54VPGEE29 pKa = 4.26GEE31 pKa = 4.21VEE33 pKa = 4.16RR34 pKa = 11.84TSATYY39 pKa = 10.31VCGDD43 pKa = 3.36RR44 pKa = 11.84EE45 pKa = 4.09IAVEE49 pKa = 3.97YY50 pKa = 10.97VNAEE54 pKa = 4.16NVSLAVLTLADD65 pKa = 3.84EE66 pKa = 4.97TIVASNVIAASGAKK80 pKa = 9.44YY81 pKa = 10.48AGAQYY86 pKa = 10.75VWWTKK91 pKa = 11.14GDD93 pKa = 3.72DD94 pKa = 3.52ADD96 pKa = 6.08FYY98 pKa = 11.74DD99 pKa = 5.42LMQGEE104 pKa = 4.61DD105 pKa = 4.41ADD107 pKa = 4.23PVSCSVKK114 pKa = 10.5GG115 pKa = 3.41

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U6BWK1|A0A4U6BWK1_9RHIZ Nuclear transport factor 2 family protein OS=Aquamicrobium sp. LC103 OX=1120658 GN=XW59_024100 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5730

0

5730

1809313

29

2846

315.8

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.35 ± 0.043

0.795 ± 0.009

5.547 ± 0.028

6.166 ± 0.03

3.907 ± 0.02

8.727 ± 0.032

2.007 ± 0.014

5.451 ± 0.022

3.113 ± 0.021

9.945 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.016

2.593 ± 0.015

5.01 ± 0.024

2.838 ± 0.015

7.233 ± 0.034

5.504 ± 0.024

5.119 ± 0.023

7.559 ± 0.022

1.29 ± 0.012

2.225 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski