Amino acid dipepetide frequency for Heliobacillus mobilis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.827AlaAla: 7.827 ± 0.125	0.938AlaCys: 0.938 ± 0.032	4.108AlaAsp: 4.108 ± 0.068	5.705AlaGlu: 5.705 ± 0.087	3.194AlaPhe: 3.194 ± 0.053	6.532AlaGly: 6.532 ± 0.094	1.48AlaHis: 1.48 ± 0.041	5.611AlaIle: 5.611 ± 0.081	4.675AlaLys: 4.675 ± 0.082	8.699AlaLeu: 8.699 ± 0.111	2.49AlaMet: 2.49 ± 0.057	2.701AlaAsn: 2.701 ± 0.055	2.685AlaPro: 2.685 ± 0.064	3.376AlaGln: 3.376 ± 0.059	4.407AlaArg: 4.407 ± 0.073	4.583AlaSer: 4.583 ± 0.082	4.035AlaThr: 4.035 ± 0.079	6.546AlaVal: 6.546 ± 0.091	0.868AlaTrp: 0.868 ± 0.026	2.352AlaTyr: 2.352 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.689CysAla: 0.689 ± 0.026	0.164CysCys: 0.164 ± 0.013	0.483CysAsp: 0.483 ± 0.023	0.54CysGlu: 0.54 ± 0.025	0.376CysPhe: 0.376 ± 0.017	1.088CysGly: 1.088 ± 0.038	0.315CysHis: 0.315 ± 0.019	0.603CysIle: 0.603 ± 0.028	0.434CysLys: 0.434 ± 0.022	0.982CysLeu: 0.982 ± 0.032	0.236CysMet: 0.236 ± 0.015	0.405CysAsn: 0.405 ± 0.02	0.697CysPro: 0.697 ± 0.031	0.415CysGln: 0.415 ± 0.019	0.731CysArg: 0.731 ± 0.03	0.685CysSer: 0.685 ± 0.027	0.548CysThr: 0.548 ± 0.024	0.585CysVal: 0.585 ± 0.03	0.13CysTrp: 0.13 ± 0.011	0.316CysTyr: 0.316 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.782AspAla: 3.782 ± 0.063	0.598AspCys: 0.598 ± 0.028	2.718AspAsp: 2.718 ± 0.052	3.782AspGlu: 3.782 ± 0.06	2.274AspPhe: 2.274 ± 0.046	3.942AspGly: 3.942 ± 0.062	1.054AspHis: 1.054 ± 0.032	3.722AspIle: 3.722 ± 0.063	2.845AspLys: 2.845 ± 0.051	5.316AspLeu: 5.316 ± 0.071	1.306AspMet: 1.306 ± 0.037	1.611AspAsn: 1.611 ± 0.04	2.469AspPro: 2.469 ± 0.051	1.788AspGln: 1.788 ± 0.048	3.083AspArg: 3.083 ± 0.055	2.673AspSer: 2.673 ± 0.058	2.454AspThr: 2.454 ± 0.052	3.92AspVal: 3.92 ± 0.056	0.662AspTrp: 0.662 ± 0.025	1.747AspTyr: 1.747 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	6.297GluAla: 6.297 ± 0.086	0.496GluCys: 0.496 ± 0.024	3.007GluAsp: 3.007 ± 0.058	5.797GluGlu: 5.797 ± 0.09	2.048GluPhe: 2.048 ± 0.051	4.484GluGly: 4.484 ± 0.081	1.165GluHis: 1.165 ± 0.03	4.747GluIle: 4.747 ± 0.072	5.218GluLys: 5.218 ± 0.068	6.67GluLeu: 6.67 ± 0.089	2.101GluMet: 2.101 ± 0.041	2.628GluAsn: 2.628 ± 0.053	2.448GluPro: 2.448 ± 0.054	3.36GluGln: 3.36 ± 0.065	4.241GluArg: 4.241 ± 0.075	3.489GluSer: 3.489 ± 0.06	3.629GluThr: 3.629 ± 0.061	4.92GluVal: 4.92 ± 0.068	0.695GluTrp: 0.695 ± 0.025	2.024GluTyr: 2.024 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	2.871PheAla: 2.871 ± 0.05	0.482PheCys: 0.482 ± 0.021	2.152PheAsp: 2.152 ± 0.042	2.138PheGlu: 2.138 ± 0.042	1.822PhePhe: 1.822 ± 0.054	3.033PheGly: 3.033 ± 0.056	0.82PheHis: 0.82 ± 0.029	2.543PheIle: 2.543 ± 0.045	1.402PheLys: 1.402 ± 0.033	3.791PheLeu: 3.791 ± 0.073	0.905PheMet: 0.905 ± 0.03	1.394PheAsn: 1.394 ± 0.04	1.636PhePro: 1.636 ± 0.04	1.469PheGln: 1.469 ± 0.037	2.048PheArg: 2.048 ± 0.044	2.767PheSer: 2.767 ± 0.06	2.269PheThr: 2.269 ± 0.051	2.596PheVal: 2.596 ± 0.048	0.492PheTrp: 0.492 ± 0.022	1.228PheTyr: 1.228 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	5.546GlyAla: 5.546 ± 0.091	0.975GlyCys: 0.975 ± 0.037	3.669GlyAsp: 3.669 ± 0.06	4.694GlyGlu: 4.694 ± 0.064	3.117GlyPhe: 3.117 ± 0.052	5.308GlyGly: 5.308 ± 0.098	1.538GlyHis: 1.538 ± 0.041	5.437GlyIle: 5.437 ± 0.08	4.836GlyLys: 4.836 ± 0.068	7.414GlyLeu: 7.414 ± 0.091	2.222GlyMet: 2.222 ± 0.039	2.665GlyAsn: 2.665 ± 0.058	2.386GlyPro: 2.386 ± 0.053	3.124GlyGln: 3.124 ± 0.068	4.109GlyArg: 4.109 ± 0.063	4.153GlySer: 4.153 ± 0.08	4.481GlyThr: 4.481 ± 0.079	5.206GlyVal: 5.206 ± 0.071	0.893GlyTrp: 0.893 ± 0.03	2.571GlyTyr: 2.571 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.24HisAla: 1.24 ± 0.031	0.298HisCys: 0.298 ± 0.016	0.944HisAsp: 0.944 ± 0.032	1.047HisGlu: 1.047 ± 0.032	0.859HisPhe: 0.859 ± 0.029	1.496HisGly: 1.496 ± 0.034	0.605HisHis: 0.605 ± 0.026	1.35HisIle: 1.35 ± 0.039	0.857HisLys: 0.857 ± 0.025	2.056HisLeu: 2.056 ± 0.046	0.483HisMet: 0.483 ± 0.021	0.651HisAsn: 0.651 ± 0.024	1.288HisPro: 1.288 ± 0.034	0.864HisGln: 0.864 ± 0.029	1.431HisArg: 1.431 ± 0.037	1.134HisSer: 1.134 ± 0.036	0.878HisThr: 0.878 ± 0.032	1.292HisVal: 1.292 ± 0.037	0.299HisTrp: 0.299 ± 0.018	0.644HisTyr: 0.644 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.86IleAla: 5.86 ± 0.073	0.729IleCys: 0.729 ± 0.024	3.956IleAsp: 3.956 ± 0.063	4.493IleGlu: 4.493 ± 0.073	2.35IlePhe: 2.35 ± 0.052	5.036IleGly: 5.036 ± 0.072	1.432IleHis: 1.432 ± 0.033	4.205IleIle: 4.205 ± 0.071	3.093IleLys: 3.093 ± 0.055	6.246IleLeu: 6.246 ± 0.086	1.435IleMet: 1.435 ± 0.038	2.461IleAsn: 2.461 ± 0.053	3.498IlePro: 3.498 ± 0.067	2.599IleGln: 2.599 ± 0.044	3.784IleArg: 3.784 ± 0.061	4.059IleSer: 4.059 ± 0.063	3.817IleThr: 3.817 ± 0.063	4.768IleVal: 4.768 ± 0.062	0.589IleTrp: 0.589 ± 0.026	1.859IleTyr: 1.859 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.926LysAla: 4.926 ± 0.067	0.39LysCys: 0.39 ± 0.023	2.987LysAsp: 2.987 ± 0.056	4.714LysGlu: 4.714 ± 0.073	1.387LysPhe: 1.387 ± 0.033	4.001LysGly: 4.001 ± 0.074	0.904LysHis: 0.904 ± 0.028	3.429LysIle: 3.429 ± 0.055	3.652LysLys: 3.652 ± 0.064	4.649LysLeu: 4.649 ± 0.066	1.568LysMet: 1.568 ± 0.04	2.239LysAsn: 2.239 ± 0.05	2.393LysPro: 2.393 ± 0.052	2.135LysGln: 2.135 ± 0.048	2.826LysArg: 2.826 ± 0.056	3.104LysSer: 3.104 ± 0.057	3.13LysThr: 3.13 ± 0.055	4.173LysVal: 4.173 ± 0.073	0.545LysTrp: 0.545 ± 0.023	1.59LysTyr: 1.59 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	9.061LeuAla: 9.061 ± 0.104	1.021LeuCys: 1.021 ± 0.033	4.991LeuAsp: 4.991 ± 0.078	6.417LeuGlu: 6.417 ± 0.086	3.909LeuPhe: 3.909 ± 0.08	6.986LeuGly: 6.986 ± 0.099	1.96LeuHis: 1.96 ± 0.048	5.959LeuIle: 5.959 ± 0.08	5.153LeuLys: 5.153 ± 0.07	10.058LeuLeu: 10.058 ± 0.139	2.418LeuMet: 2.418 ± 0.054	3.696LeuAsn: 3.696 ± 0.055	4.674LeuPro: 4.674 ± 0.076	4.062LeuGln: 4.062 ± 0.073	5.618LeuArg: 5.618 ± 0.08	6.901LeuSer: 6.901 ± 0.087	6.035LeuThr: 6.035 ± 0.081	6.531LeuVal: 6.531 ± 0.085	1.088LeuTrp: 1.088 ± 0.033	2.722LeuTyr: 2.722 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.679MetAla: 2.679 ± 0.055	0.179MetCys: 0.179 ± 0.013	1.594MetAsp: 1.594 ± 0.034	1.968MetGlu: 1.968 ± 0.042	0.773MetPhe: 0.773 ± 0.026	2.032MetGly: 2.032 ± 0.043	0.401MetHis: 0.401 ± 0.021	1.698MetIle: 1.698 ± 0.041	1.54MetLys: 1.54 ± 0.036	2.243MetLeu: 2.243 ± 0.05	0.742MetMet: 0.742 ± 0.027	1.241MetAsn: 1.241 ± 0.033	1.108MetPro: 1.108 ± 0.033	0.909MetGln: 0.909 ± 0.029	1.301MetArg: 1.301 ± 0.033	1.506MetSer: 1.506 ± 0.038	1.697MetThr: 1.697 ± 0.035	2.08MetVal: 2.08 ± 0.044	0.143MetTrp: 0.143 ± 0.011	0.556MetTyr: 0.556 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.667AsnAla: 2.667 ± 0.056	0.415AsnCys: 0.415 ± 0.02	1.743AsnAsp: 1.743 ± 0.04	2.196AsnGlu: 2.196 ± 0.046	1.204AsnPhe: 1.204 ± 0.034	2.787AsnGly: 2.787 ± 0.06	0.823AsnHis: 0.823 ± 0.027	2.516AsnIle: 2.516 ± 0.057	1.784AsnLys: 1.784 ± 0.044	3.856AsnLeu: 3.856 ± 0.063	0.895AsnMet: 0.895 ± 0.027	1.382AsnAsn: 1.382 ± 0.042	2.146AsnPro: 2.146 ± 0.041	1.648AsnGln: 1.648 ± 0.038	2.427AsnArg: 2.427 ± 0.048	2.092AsnSer: 2.092 ± 0.047	1.776AsnThr: 1.776 ± 0.045	2.634AsnVal: 2.634 ± 0.053	0.407AsnTrp: 0.407 ± 0.019	1.195AsnTyr: 1.195 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	3.372ProAla: 3.372 ± 0.071	0.417ProCys: 0.417 ± 0.025	2.513ProAsp: 2.513 ± 0.053	3.683ProGlu: 3.683 ± 0.06	1.866ProPhe: 1.866 ± 0.04	3.352ProGly: 3.352 ± 0.07	0.845ProHis: 0.845 ± 0.029	2.681ProIle: 2.681 ± 0.052	2.198ProLys: 2.198 ± 0.047	4.181ProLeu: 4.181 ± 0.069	1.079ProMet: 1.079 ± 0.032	1.527ProAsn: 1.527 ± 0.036	1.875ProPro: 1.875 ± 0.057	1.675ProGln: 1.675 ± 0.045	1.975ProArg: 1.975 ± 0.045	2.724ProSer: 2.724 ± 0.061	2.272ProThr: 2.272 ± 0.05	3.912ProVal: 3.912 ± 0.067	0.605ProTrp: 0.605 ± 0.026	1.351ProTyr: 1.351 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.783GlnAla: 3.783 ± 0.065	0.316GlnCys: 0.316 ± 0.016	1.663GlnAsp: 1.663 ± 0.038	3.137GlnGlu: 3.137 ± 0.064	1.234GlnPhe: 1.234 ± 0.031	3.006GlnGly: 3.006 ± 0.053	0.674GlnHis: 0.674 ± 0.024	2.641GlnIle: 2.641 ± 0.049	2.608GlnLys: 2.608 ± 0.05	3.839GlnLeu: 3.839 ± 0.067	1.252GlnMet: 1.252 ± 0.035	1.449GlnAsn: 1.449 ± 0.039	1.662GlnPro: 1.662 ± 0.04	1.837GlnGln: 1.837 ± 0.05	2.519GlnArg: 2.519 ± 0.055	2.244GlnSer: 2.244 ± 0.049	2.065GlnThr: 2.065 ± 0.05	3.318GlnVal: 3.318 ± 0.05	0.56GlnTrp: 0.56 ± 0.022	1.093GlnTyr: 1.093 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	3.754ArgAla: 3.754 ± 0.064	0.654ArgCys: 0.654 ± 0.028	2.764ArgAsp: 2.764 ± 0.049	4.302ArgGlu: 4.302 ± 0.08	2.469ArgPhe: 2.469 ± 0.054	3.543ArgGly: 3.543 ± 0.064	1.184ArgHis: 1.184 ± 0.033	3.922ArgIle: 3.922 ± 0.06	3.195ArgLys: 3.195 ± 0.052	6.128ArgLeu: 6.128 ± 0.087	1.628ArgMet: 1.628 ± 0.043	2.028ArgAsn: 2.028 ± 0.04	2.318ArgPro: 2.318 ± 0.07	2.771ArgGln: 2.771 ± 0.061	3.669ArgArg: 3.669 ± 0.072	3.028ArgSer: 3.028 ± 0.054	2.667ArgThr: 2.667 ± 0.047	3.903ArgVal: 3.903 ± 0.06	0.848ArgTrp: 0.848 ± 0.028	1.844ArgTyr: 1.844 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.523SerAla: 4.523 ± 0.072	0.532SerCys: 0.532 ± 0.025	2.963SerAsp: 2.963 ± 0.049	3.65SerGlu: 3.65 ± 0.064	2.463SerPhe: 2.463 ± 0.056	4.84SerGly: 4.84 ± 0.079	1.141SerHis: 1.141 ± 0.034	3.933SerIle: 3.933 ± 0.079	2.958SerLys: 2.958 ± 0.054	6.4SerLeu: 6.4 ± 0.08	1.58SerMet: 1.58 ± 0.037	2.07SerAsn: 2.07 ± 0.048	2.82SerPro: 2.82 ± 0.06	2.378SerGln: 2.378 ± 0.054	3.286SerArg: 3.286 ± 0.056	3.984SerSer: 3.984 ± 0.089	3.298SerThr: 3.298 ± 0.075	4.205SerVal: 4.205 ± 0.071	0.666SerTrp: 0.666 ± 0.025	1.56SerTyr: 1.56 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	4.749ThrAla: 4.749 ± 0.087	0.501ThrCys: 0.501 ± 0.021	2.838ThrAsp: 2.838 ± 0.059	3.521ThrGlu: 3.521 ± 0.061	2.122ThrPhe: 2.122 ± 0.046	4.795ThrGly: 4.795 ± 0.078	1.004ThrHis: 1.004 ± 0.036	3.742ThrIle: 3.742 ± 0.066	2.396ThrLys: 2.396 ± 0.051	5.334ThrLeu: 5.334 ± 0.078	1.342ThrMet: 1.342 ± 0.034	1.971ThrAsn: 1.971 ± 0.054	2.716ThrPro: 2.716 ± 0.051	1.786ThrGln: 1.786 ± 0.04	2.432ThrArg: 2.432 ± 0.054	3.263ThrSer: 3.263 ± 0.074	3.124ThrThr: 3.124 ± 0.086	4.997ThrVal: 4.997 ± 0.096	0.564ThrTrp: 0.564 ± 0.023	1.639ThrTyr: 1.639 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	6.326ValAla: 6.326 ± 0.092	0.81ValCys: 0.81 ± 0.027	4.372ValAsp: 4.372 ± 0.072	5.175ValGlu: 5.175 ± 0.073	2.699ValPhe: 2.699 ± 0.055	4.894ValGly: 4.894 ± 0.077	1.454ValHis: 1.454 ± 0.032	5.04ValIle: 5.04 ± 0.065	4.021ValLys: 4.021 ± 0.067	6.989ValLeu: 6.989 ± 0.082	1.773ValMet: 1.773 ± 0.036	2.931ValAsn: 2.931 ± 0.051	3.383ValPro: 3.383 ± 0.062	2.843ValGln: 2.843 ± 0.055	3.932ValArg: 3.932 ± 0.058	4.446ValSer: 4.446 ± 0.066	4.609ValThr: 4.609 ± 0.083	5.497ValVal: 5.497 ± 0.085	0.607ValTrp: 0.607 ± 0.026	2.003ValTyr: 2.003 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.791TrpAla: 0.791 ± 0.028	0.101TrpCys: 0.101 ± 0.011	0.656TrpAsp: 0.656 ± 0.027	0.776TrpGlu: 0.776 ± 0.026	0.437TrpPhe: 0.437 ± 0.02	0.759TrpGly: 0.759 ± 0.027	0.203TrpHis: 0.203 ± 0.014	0.7TrpIle: 0.7 ± 0.025	0.628TrpLys: 0.628 ± 0.024	1.213TrpLeu: 1.213 ± 0.038	0.356TrpMet: 0.356 ± 0.018	0.479TrpAsn: 0.479 ± 0.02	0.429TrpPro: 0.429 ± 0.021	0.549TrpGln: 0.549 ± 0.022	0.635TrpArg: 0.635 ± 0.021	0.675TrpSer: 0.675 ± 0.027	0.535TrpThr: 0.535 ± 0.023	0.753TrpVal: 0.753 ± 0.03	0.163TrpTrp: 0.163 ± 0.013	0.361TrpTyr: 0.361 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.066TyrAla: 2.066 ± 0.045	0.407TyrCys: 0.407 ± 0.02	1.622TyrAsp: 1.622 ± 0.041	1.727TyrGlu: 1.727 ± 0.046	1.257TyrPhe: 1.257 ± 0.038	2.429TyrGly: 2.429 ± 0.046	0.717TyrHis: 0.717 ± 0.024	1.791TyrIle: 1.791 ± 0.04	1.249TyrLys: 1.249 ± 0.037	3.196TyrLeu: 3.196 ± 0.054	0.579TyrMet: 0.579 ± 0.024	1.066TyrAsn: 1.066 ± 0.033	1.471TyrPro: 1.471 ± 0.041	1.311TyrGln: 1.311 ± 0.034	2.163TyrArg: 2.163 ± 0.046	1.74TyrSer: 1.74 ± 0.041	1.519TyrThr: 1.519 ± 0.038	1.967TyrVal: 1.967 ± 0.047	0.376TyrTrp: 0.376 ± 0.019	1.016TyrTyr: 1.016 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3745 proteins (1150309 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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