Salmonella phage Shemara

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Cornellvirus; unclassified Cornellvirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8RRY6|A0A5B8RRY6_9CAUD Uncharacterized protein OS=Salmonella phage Shemara OX=2596714 GN=CPT_Shemara_076 PE=4 SV=1
MM1 pKa = 6.5VARR4 pKa = 11.84YY5 pKa = 9.06RR6 pKa = 11.84FNYY9 pKa = 7.46VQEE12 pKa = 4.15MFEE15 pKa = 4.17TDD17 pKa = 3.69AGEE20 pKa = 4.25QYY22 pKa = 11.04CEE24 pKa = 4.14TEE26 pKa = 3.49RR27 pKa = 11.84DD28 pKa = 3.19EE29 pKa = 4.15WVKK32 pKa = 11.35YY33 pKa = 10.15EE34 pKa = 4.94DD35 pKa = 3.89YY36 pKa = 11.62AEE38 pKa = 4.18LRR40 pKa = 11.84SLLFRR45 pKa = 11.84MTDD48 pKa = 3.11SVNLSEE54 pKa = 5.2LPEE57 pKa = 4.13GLYY60 pKa = 10.83DD61 pKa = 4.1DD62 pKa = 5.45LEE64 pKa = 5.3ARR66 pKa = 11.84GLLL69 pKa = 3.84

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8RMV4|A0A5B8RMV4_9CAUD Uncharacterized protein OS=Salmonella phage Shemara OX=2596714 GN=CPT_Shemara_021 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84LTPKK6 pKa = 10.29EE7 pKa = 3.79PEE9 pKa = 3.64PRR11 pKa = 11.84KK12 pKa = 10.19RR13 pKa = 11.84CIQCRR18 pKa = 11.84KK19 pKa = 9.72RR20 pKa = 11.84CIQCRR25 pKa = 11.84EE26 pKa = 4.06RR27 pKa = 11.84LPLSAFHH34 pKa = 6.84KK35 pKa = 10.58SSVASIDD42 pKa = 3.61GYY44 pKa = 11.66RR45 pKa = 11.84NICKK49 pKa = 9.56SCRR52 pKa = 11.84RR53 pKa = 11.84ATEE56 pKa = 3.53AARR59 pKa = 11.84IRR61 pKa = 11.84EE62 pKa = 4.17KK63 pKa = 10.84RR64 pKa = 11.84NKK66 pKa = 9.7

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

14110

29

850

170.0

18.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.851 ± 0.52

1.297 ± 0.146

6.201 ± 0.27

6.584 ± 0.378

3.65 ± 0.178

7.605 ± 0.276

1.772 ± 0.203

5.046 ± 0.195

5.932 ± 0.337

7.718 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.157

4.805 ± 0.328

3.692 ± 0.216

3.969 ± 0.311

5.514 ± 0.246

5.776 ± 0.305

6.095 ± 0.394

6.875 ± 0.318

1.517 ± 0.146

3.444 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski