Lutibacter profundi

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Lutibacter

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2525 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X8G650|A0A0X8G650_9FLAO Dihydrofolate reductase OS=Lutibacter profundi OX=1622118 GN=Lupro_05960 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.43KK3 pKa = 10.11IINKK7 pKa = 9.46LLIIALVFSTVMSCKK22 pKa = 10.74DD23 pKa = 3.88PDD25 pKa = 3.49NAIYY29 pKa = 10.61DD30 pKa = 3.84VFDD33 pKa = 3.93GVSYY37 pKa = 10.64GAVIRR42 pKa = 11.84TLDD45 pKa = 3.44RR46 pKa = 11.84VSTNYY51 pKa = 10.78NIFDD55 pKa = 4.06LNSRR59 pKa = 11.84FEE61 pKa = 4.29IVVEE65 pKa = 4.25EE66 pKa = 3.91QDD68 pKa = 3.39EE69 pKa = 4.82EE70 pKa = 4.77YY71 pKa = 11.16GGLLDD76 pKa = 4.06KK77 pKa = 11.59VNVYY81 pKa = 9.85VSYY84 pKa = 10.8TDD86 pKa = 3.35KK87 pKa = 11.32TDD89 pKa = 4.33DD90 pKa = 3.8GVNNSKK96 pKa = 11.25AEE98 pKa = 3.92ILLKK102 pKa = 10.75SIAASEE108 pKa = 4.41FTTSANGLPSTTIAATFSEE127 pKa = 4.46ALAALGFTIGDD138 pKa = 3.6GNYY141 pKa = 10.5NGGDD145 pKa = 3.24EE146 pKa = 4.39FDD148 pKa = 4.1FRR150 pKa = 11.84LEE152 pKa = 4.06VVLTDD157 pKa = 3.61GRR159 pKa = 11.84SFSADD164 pKa = 3.41DD165 pKa = 4.37ASGSLQGSYY174 pKa = 10.42FKK176 pKa = 10.9SPYY179 pKa = 9.68AYY181 pKa = 10.36NVGILCIPDD190 pKa = 3.44SPITGDD196 pKa = 3.65YY197 pKa = 9.85VINMQDD203 pKa = 2.82SYY205 pKa = 11.89GDD207 pKa = 3.25GWQGSKK213 pKa = 9.96IVCTIDD219 pKa = 3.54GVEE222 pKa = 4.45NYY224 pKa = 10.87AFLPDD229 pKa = 3.67YY230 pKa = 9.93WSTGLGPFTDD240 pKa = 4.34GTATITVPAGASTVVWSFVAGDD262 pKa = 3.66WPSEE266 pKa = 3.82VSFQIIGPNSGNVIGDD282 pKa = 4.11FGPSPVEE289 pKa = 4.35GEE291 pKa = 4.36LALNLCNEE299 pKa = 4.33

Molecular weight: 32.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A109RMQ9|A0A109RMQ9_9FLAO Cystathionine beta-synthase OS=Lutibacter profundi OX=1622118 GN=Lupro_00950 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.24RR22 pKa = 11.84MASATGRR29 pKa = 11.84KK30 pKa = 8.64VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.03GRR40 pKa = 11.84KK41 pKa = 7.95NLSVSSAPRR50 pKa = 11.84PKK52 pKa = 10.38KK53 pKa = 10.5

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2525

0

2525

871124

49

3371

345.0

39.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.866 ± 0.045

0.701 ± 0.019

4.855 ± 0.041

6.352 ± 0.047

5.346 ± 0.045

6.144 ± 0.053

1.668 ± 0.021

9.115 ± 0.048

9.014 ± 0.075

9.236 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.024

6.978 ± 0.049

3.196 ± 0.023

3.093 ± 0.028

3.049 ± 0.03

6.395 ± 0.04

5.933 ± 0.062

5.96 ± 0.038

0.979 ± 0.016

4.084 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski