Amino acid dipepetide frequency for Rufibacter sp. DG15C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.759AlaAla: 6.759 ± 0.111
0.686AlaCys: 0.686 ± 0.026
3.886AlaAsp: 3.886 ± 0.074
4.664AlaGlu: 4.664 ± 0.08
3.832AlaPhe: 3.832 ± 0.065
6.008AlaGly: 6.008 ± 0.105
1.602AlaHis: 1.602 ± 0.042
4.88AlaIle: 4.88 ± 0.074
4.787AlaLys: 4.787 ± 0.083
8.333AlaLeu: 8.333 ± 0.097
2.078AlaMet: 2.078 ± 0.049
3.438AlaAsn: 3.438 ± 0.081
3.318AlaPro: 3.318 ± 0.085
4.226AlaGln: 4.226 ± 0.077
3.184AlaArg: 3.184 ± 0.061
5.079AlaSer: 5.079 ± 0.094
4.762AlaThr: 4.762 ± 0.099
5.398AlaVal: 5.398 ± 0.076
0.884AlaTrp: 0.884 ± 0.029
2.871AlaTyr: 2.871 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.562CysAla: 0.562 ± 0.025
0.09CysCys: 0.09 ± 0.009
0.362CysAsp: 0.362 ± 0.019
0.414CysGlu: 0.414 ± 0.023
0.346CysPhe: 0.346 ± 0.017
0.637CysGly: 0.637 ± 0.05
0.186CysHis: 0.186 ± 0.012
0.435CysIle: 0.435 ± 0.022
0.308CysLys: 0.308 ± 0.015
0.818CysLeu: 0.818 ± 0.027
0.135CysMet: 0.135 ± 0.012
0.273CysAsn: 0.273 ± 0.016
0.349CysPro: 0.349 ± 0.02
0.363CysGln: 0.363 ± 0.02
0.302CysArg: 0.302 ± 0.02
0.471CysSer: 0.471 ± 0.021
0.466CysThr: 0.466 ± 0.038
0.438CysVal: 0.438 ± 0.022
0.069CysTrp: 0.069 ± 0.008
0.219CysTyr: 0.219 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.044AspAla: 4.044 ± 0.079
0.333AspCys: 0.333 ± 0.018
2.073AspAsp: 2.073 ± 0.052
3.044AspGlu: 3.044 ± 0.056
2.872AspPhe: 2.872 ± 0.053
3.421AspGly: 3.421 ± 0.068
0.945AspHis: 0.945 ± 0.032
3.154AspIle: 3.154 ± 0.06
3.22AspLys: 3.22 ± 0.061
5.249AspLeu: 5.249 ± 0.078
1.227AspMet: 1.227 ± 0.033
2.123AspAsn: 2.123 ± 0.043
2.071AspPro: 2.071 ± 0.046
2.137AspGln: 2.137 ± 0.056
2.237AspArg: 2.237 ± 0.05
2.697AspSer: 2.697 ± 0.063
2.659AspThr: 2.659 ± 0.054
3.564AspVal: 3.564 ± 0.061
0.649AspTrp: 0.649 ± 0.027
2.238AspTyr: 2.238 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
5.345GluAla: 5.345 ± 0.081
0.302GluCys: 0.302 ± 0.019
3.219GluAsp: 3.219 ± 0.062
4.939GluGlu: 4.939 ± 0.091
2.512GluPhe: 2.512 ± 0.051
3.91GluGly: 3.91 ± 0.064
1.226GluHis: 1.226 ± 0.035
3.898GluIle: 3.898 ± 0.066
4.459GluLys: 4.459 ± 0.075
6.084GluLeu: 6.084 ± 0.102
1.598GluMet: 1.598 ± 0.041
3.018GluAsn: 3.018 ± 0.056
2.025GluPro: 2.025 ± 0.049
3.05GluGln: 3.05 ± 0.053
3.082GluArg: 3.082 ± 0.054
2.962GluSer: 2.962 ± 0.053
3.206GluThr: 3.206 ± 0.056
4.915GluVal: 4.915 ± 0.069
0.661GluTrp: 0.661 ± 0.025
2.082GluTyr: 2.082 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.356PheAla: 3.356 ± 0.065
0.41PheCys: 0.41 ± 0.017
2.582PheAsp: 2.582 ± 0.057
2.639PheGlu: 2.639 ± 0.052
2.33PhePhe: 2.33 ± 0.066
3.337PheGly: 3.337 ± 0.07
0.857PheHis: 0.857 ± 0.032
2.599PheIle: 2.599 ± 0.061
2.857PheLys: 2.857 ± 0.054
4.976PheLeu: 4.976 ± 0.095
1.068PheMet: 1.068 ± 0.036
2.182PheAsn: 2.182 ± 0.05
1.814PhePro: 1.814 ± 0.044
1.942PheGln: 1.942 ± 0.061
1.74PheArg: 1.74 ± 0.048
3.405PheSer: 3.405 ± 0.064
3.264PheThr: 3.264 ± 0.079
2.958PheVal: 2.958 ± 0.064
0.638PheTrp: 0.638 ± 0.029
1.9PheTyr: 1.9 ± 0.048
0.0PheXaa: 0.0 ± 0.0
Gly
5.122GlyAla: 5.122 ± 0.084
0.642GlyCys: 0.642 ± 0.069
3.146GlyAsp: 3.146 ± 0.054
3.686GlyGlu: 3.686 ± 0.063
3.54GlyPhe: 3.54 ± 0.069
5.316GlyGly: 5.316 ± 0.119
1.408GlyHis: 1.408 ± 0.042
4.648GlyIle: 4.648 ± 0.073
4.88GlyLys: 4.88 ± 0.074
6.739GlyLeu: 6.739 ± 0.101
1.852GlyMet: 1.852 ± 0.045
3.159GlyAsn: 3.159 ± 0.073
2.006GlyPro: 2.006 ± 0.067
3.048GlyGln: 3.048 ± 0.06
2.884GlyArg: 2.884 ± 0.055
4.147GlySer: 4.147 ± 0.082
4.578GlyThr: 4.578 ± 0.128
4.914GlyVal: 4.914 ± 0.076
0.873GlyTrp: 0.873 ± 0.03
2.905GlyTyr: 2.905 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.496HisAla: 1.496 ± 0.037
0.184HisCys: 0.184 ± 0.014
0.967HisAsp: 0.967 ± 0.034
1.1HisGlu: 1.1 ± 0.031
1.087HisPhe: 1.087 ± 0.034
1.347HisGly: 1.347 ± 0.039
0.637HisHis: 0.637 ± 0.029
1.173HisIle: 1.173 ± 0.037
1.012HisLys: 1.012 ± 0.035
2.419HisLeu: 2.419 ± 0.055
0.437HisMet: 0.437 ± 0.02
0.798HisAsn: 0.798 ± 0.028
1.189HisPro: 1.189 ± 0.034
1.038HisGln: 1.038 ± 0.035
0.958HisArg: 0.958 ± 0.032
1.134HisSer: 1.134 ± 0.029
1.134HisThr: 1.134 ± 0.032
1.272HisVal: 1.272 ± 0.039
0.257HisTrp: 0.257 ± 0.016
0.919HisTyr: 0.919 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
4.881IleAla: 4.881 ± 0.068
0.57IleCys: 0.57 ± 0.025
3.242IleAsp: 3.242 ± 0.065
3.659IleGlu: 3.659 ± 0.072
2.427IlePhe: 2.427 ± 0.053
4.524IleGly: 4.524 ± 0.087
1.169IleHis: 1.169 ± 0.032
3.666IleIle: 3.666 ± 0.077
3.727IleLys: 3.727 ± 0.061
5.738IleLeu: 5.738 ± 0.094
1.281IleMet: 1.281 ± 0.043
2.725IleAsn: 2.725 ± 0.057
2.701IlePro: 2.701 ± 0.055
2.474IleGln: 2.474 ± 0.045
2.496IleArg: 2.496 ± 0.044
4.0IleSer: 4.0 ± 0.064
3.932IleThr: 3.932 ± 0.085
3.458IleVal: 3.458 ± 0.061
0.589IleTrp: 0.589 ± 0.028
1.959IleTyr: 1.959 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
5.323LysAla: 5.323 ± 0.08
0.249LysCys: 0.249 ± 0.016
3.59LysAsp: 3.59 ± 0.082
4.668LysGlu: 4.668 ± 0.089
2.217LysPhe: 2.217 ± 0.048
4.203LysGly: 4.203 ± 0.067
1.293LysHis: 1.293 ± 0.034
3.61LysIle: 3.61 ± 0.065
4.29LysLys: 4.29 ± 0.091
5.633LysLeu: 5.633 ± 0.076
1.627LysMet: 1.627 ± 0.041
3.06LysAsn: 3.06 ± 0.055
2.673LysPro: 2.673 ± 0.059
2.66LysGln: 2.66 ± 0.056
2.775LysArg: 2.775 ± 0.061
3.314LysSer: 3.314 ± 0.06
3.695LysThr: 3.695 ± 0.069
4.514LysVal: 4.514 ± 0.08
0.662LysTrp: 0.662 ± 0.026
2.174LysTyr: 2.174 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
8.427LeuAla: 8.427 ± 0.11
0.788LeuCys: 0.788 ± 0.029
5.064LeuAsp: 5.064 ± 0.084
6.385LeuGlu: 6.385 ± 0.095
4.629LeuPhe: 4.629 ± 0.095
6.589LeuGly: 6.589 ± 0.094
2.198LeuHis: 2.198 ± 0.056
5.264LeuIle: 5.264 ± 0.099
6.158LeuLys: 6.158 ± 0.08
11.004LeuLeu: 11.004 ± 0.162
2.328LeuMet: 2.328 ± 0.055
4.544LeuAsn: 4.544 ± 0.074
4.936LeuPro: 4.936 ± 0.07
5.101LeuGln: 5.101 ± 0.086
4.501LeuArg: 4.501 ± 0.085
6.65LeuSer: 6.65 ± 0.095
6.076LeuThr: 6.076 ± 0.09
7.03LeuVal: 7.03 ± 0.094
1.046LeuTrp: 1.046 ± 0.032
3.301LeuTyr: 3.301 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.278MetAla: 2.278 ± 0.047
0.117MetCys: 0.117 ± 0.01
1.227MetAsp: 1.227 ± 0.033
1.52MetGlu: 1.52 ± 0.045
0.815MetPhe: 0.815 ± 0.034
1.736MetGly: 1.736 ± 0.047
0.514MetHis: 0.514 ± 0.023
1.251MetIle: 1.251 ± 0.037
1.738MetLys: 1.738 ± 0.038
2.358MetLeu: 2.358 ± 0.045
0.661MetMet: 0.661 ± 0.03
1.036MetAsn: 1.036 ± 0.032
1.14MetPro: 1.14 ± 0.032
1.137MetGln: 1.137 ± 0.036
1.143MetArg: 1.143 ± 0.034
1.374MetSer: 1.374 ± 0.035
1.247MetThr: 1.247 ± 0.038
1.742MetVal: 1.742 ± 0.042
0.164MetTrp: 0.164 ± 0.013
0.623MetTyr: 0.623 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.577AsnAla: 3.577 ± 0.055
0.307AsnCys: 0.307 ± 0.018
1.913AsnAsp: 1.913 ± 0.046
2.378AsnGlu: 2.378 ± 0.052
2.061AsnPhe: 2.061 ± 0.048
3.494AsnGly: 3.494 ± 0.135
0.865AsnHis: 0.865 ± 0.031
2.815AsnIle: 2.815 ± 0.054
2.541AsnLys: 2.541 ± 0.056
4.551AsnLeu: 4.551 ± 0.069
1.09AsnMet: 1.09 ± 0.036
2.127AsnAsn: 2.127 ± 0.065
2.651AsnPro: 2.651 ± 0.058
2.041AsnGln: 2.041 ± 0.044
2.234AsnArg: 2.234 ± 0.049
2.657AsnSer: 2.657 ± 0.052
2.759AsnThr: 2.759 ± 0.07
2.986AsnVal: 2.986 ± 0.065
0.621AsnTrp: 0.621 ± 0.026
1.863AsnTyr: 1.863 ± 0.045
0.0AsnXaa: 0.0 ± 0.0
Pro
3.932ProAla: 3.932 ± 0.08
0.224ProCys: 0.224 ± 0.014
2.459ProAsp: 2.459 ± 0.057
3.567ProGlu: 3.567 ± 0.067
2.135ProPhe: 2.135 ± 0.047
2.681ProGly: 2.681 ± 0.056
0.886ProHis: 0.886 ± 0.029
2.325ProIle: 2.325 ± 0.055
2.451ProLys: 2.451 ± 0.058
3.759ProLeu: 3.759 ± 0.066
0.879ProMet: 0.879 ± 0.028
2.024ProAsn: 2.024 ± 0.045
1.165ProPro: 1.165 ± 0.044
1.694ProGln: 1.694 ± 0.043
1.359ProArg: 1.359 ± 0.041
2.658ProSer: 2.658 ± 0.052
2.553ProThr: 2.553 ± 0.051
3.368ProVal: 3.368 ± 0.063
0.439ProTrp: 0.439 ± 0.02
1.591ProTyr: 1.591 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
3.927GlnAla: 3.927 ± 0.075
0.211GlnCys: 0.211 ± 0.015
2.342GlnAsp: 2.342 ± 0.047
3.638GlnGlu: 3.638 ± 0.066
1.858GlnPhe: 1.858 ± 0.042
2.668GlnGly: 2.668 ± 0.052
1.033GlnHis: 1.033 ± 0.04
2.553GlnIle: 2.553 ± 0.049
3.146GlnLys: 3.146 ± 0.06
4.709GlnLeu: 4.709 ± 0.08
1.122GlnMet: 1.122 ± 0.035
2.156GlnAsn: 2.156 ± 0.053
1.866GlnPro: 1.866 ± 0.045
2.592GlnGln: 2.592 ± 0.076
2.02GlnArg: 2.02 ± 0.051
2.174GlnSer: 2.174 ± 0.047
2.511GlnThr: 2.511 ± 0.055
3.38GlnVal: 3.38 ± 0.051
0.488GlnTrp: 0.488 ± 0.022
1.684GlnTyr: 1.684 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
2.946ArgAla: 2.946 ± 0.059
0.249ArgCys: 0.249 ± 0.014
2.066ArgAsp: 2.066 ± 0.046
2.82ArgGlu: 2.82 ± 0.068
2.252ArgPhe: 2.252 ± 0.048
2.455ArgGly: 2.455 ± 0.05
0.888ArgHis: 0.888 ± 0.032
2.9ArgIle: 2.9 ± 0.054
2.79ArgLys: 2.79 ± 0.051
4.605ArgLeu: 4.605 ± 0.073
1.195ArgMet: 1.195 ± 0.036
2.06ArgAsn: 2.06 ± 0.049
1.623ArgPro: 1.623 ± 0.047
2.049ArgGln: 2.049 ± 0.054
2.038ArgArg: 2.038 ± 0.046
2.2ArgSer: 2.2 ± 0.042
2.407ArgThr: 2.407 ± 0.044
3.009ArgVal: 3.009 ± 0.057
0.57ArgTrp: 0.57 ± 0.024
1.777ArgTyr: 1.777 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
4.601SerAla: 4.601 ± 0.069
0.512SerCys: 0.512 ± 0.026
2.705SerAsp: 2.705 ± 0.053
3.238SerGlu: 3.238 ± 0.065
3.361SerPhe: 3.361 ± 0.061
4.548SerGly: 4.548 ± 0.076
1.194SerHis: 1.194 ± 0.036
3.773SerIle: 3.773 ± 0.07
3.488SerLys: 3.488 ± 0.065
6.34SerLeu: 6.34 ± 0.089
1.378SerMet: 1.378 ± 0.037
2.685SerAsn: 2.685 ± 0.065
2.687SerPro: 2.687 ± 0.047
2.448SerGln: 2.448 ± 0.05
2.405SerArg: 2.405 ± 0.052
3.933SerSer: 3.933 ± 0.075
3.683SerThr: 3.683 ± 0.099
4.084SerVal: 4.084 ± 0.064
0.658SerTrp: 0.658 ± 0.022
2.333SerTyr: 2.333 ± 0.056
0.0SerXaa: 0.0 ± 0.0
Thr
4.979ThrAla: 4.979 ± 0.123
0.436ThrCys: 0.436 ± 0.046
3.018ThrAsp: 3.018 ± 0.062
3.266ThrGlu: 3.266 ± 0.058
2.969ThrPhe: 2.969 ± 0.087
4.648ThrGly: 4.648 ± 0.111
1.188ThrHis: 1.188 ± 0.035
3.721ThrIle: 3.721 ± 0.082
3.191ThrLys: 3.191 ± 0.053
6.105ThrLeu: 6.105 ± 0.1
1.108ThrMet: 1.108 ± 0.032
2.528ThrAsn: 2.528 ± 0.061
3.063ThrPro: 3.063 ± 0.067
2.485ThrGln: 2.485 ± 0.046
2.141ThrArg: 2.141 ± 0.048
3.771ThrSer: 3.771 ± 0.084
3.818ThrThr: 3.818 ± 0.091
4.552ThrVal: 4.552 ± 0.12
0.752ThrTrp: 0.752 ± 0.031
2.408ThrTyr: 2.408 ± 0.086
0.0ThrXaa: 0.0 ± 0.0
Val
5.555ValAla: 5.555 ± 0.073
0.561ValCys: 0.561 ± 0.029
3.487ValAsp: 3.487 ± 0.065
4.284ValGlu: 4.284 ± 0.066
3.168ValPhe: 3.168 ± 0.064
4.64ValGly: 4.64 ± 0.077
1.337ValHis: 1.337 ± 0.039
4.104ValIle: 4.104 ± 0.07
4.341ValLys: 4.341 ± 0.079
7.235ValLeu: 7.235 ± 0.107
1.7ValMet: 1.7 ± 0.044
3.201ValAsn: 3.201 ± 0.058
2.957ValPro: 2.957 ± 0.055
3.013ValGln: 3.013 ± 0.055
3.038ValArg: 3.038 ± 0.055
4.375ValSer: 4.375 ± 0.069
4.388ValThr: 4.388 ± 0.121
5.214ValVal: 5.214 ± 0.088
0.742ValTrp: 0.742 ± 0.027
2.506ValTyr: 2.506 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.858TrpAla: 0.858 ± 0.03
0.089TrpCys: 0.089 ± 0.01
0.618TrpAsp: 0.618 ± 0.028
0.645TrpGlu: 0.645 ± 0.025
0.499TrpPhe: 0.499 ± 0.027
0.76TrpGly: 0.76 ± 0.025
0.286TrpHis: 0.286 ± 0.016
0.558TrpIle: 0.558 ± 0.022
0.633TrpLys: 0.633 ± 0.027
1.337TrpLeu: 1.337 ± 0.041
0.327TrpMet: 0.327 ± 0.018
0.529TrpAsn: 0.529 ± 0.022
0.348TrpPro: 0.348 ± 0.018
0.653TrpGln: 0.653 ± 0.028
0.568TrpArg: 0.568 ± 0.025
0.626TrpSer: 0.626 ± 0.026
0.618TrpThr: 0.618 ± 0.026
0.845TrpVal: 0.845 ± 0.031
0.164TrpTrp: 0.164 ± 0.014
0.416TrpTyr: 0.416 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.708TyrAla: 2.708 ± 0.049
0.285TyrCys: 0.285 ± 0.017
1.989TyrAsp: 1.989 ± 0.045
1.94TyrGlu: 1.94 ± 0.046
1.944TyrPhe: 1.944 ± 0.047
2.57TyrGly: 2.57 ± 0.053
0.845TyrHis: 0.845 ± 0.028
1.889TyrIle: 1.889 ± 0.042
2.212TyrLys: 2.212 ± 0.048
4.025TyrLeu: 4.025 ± 0.064
0.731TyrMet: 0.731 ± 0.031
1.864TyrAsn: 1.864 ± 0.042
1.566TyrPro: 1.566 ± 0.037
1.927TyrGln: 1.927 ± 0.045
1.792TyrArg: 1.792 ± 0.048
2.429TyrSer: 2.429 ± 0.077
2.395TyrThr: 2.395 ± 0.077
2.205TyrVal: 2.205 ± 0.04
0.452TyrTrp: 0.452 ± 0.024
1.584TyrTyr: 1.584 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3169 proteins (1046099 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski