Amino acid dipepetide frequency for Ruminococcus sp. AF41-9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.839AlaAla: 6.839 ± 0.119	1.076AlaCys: 1.076 ± 0.034	4.713AlaAsp: 4.713 ± 0.069	5.646AlaGlu: 5.646 ± 0.074	2.84AlaPhe: 2.84 ± 0.052	5.802AlaGly: 5.802 ± 0.089	1.097AlaHis: 1.097 ± 0.031	4.603AlaIle: 4.603 ± 0.072	4.734AlaLys: 4.734 ± 0.082	6.275AlaLeu: 6.275 ± 0.076	2.313AlaMet: 2.313 ± 0.05	2.407AlaAsn: 2.407 ± 0.052	1.976AlaPro: 1.976 ± 0.045	2.504AlaGln: 2.504 ± 0.053	2.853AlaArg: 2.853 ± 0.057	3.787AlaSer: 3.787 ± 0.062	3.206AlaThr: 3.206 ± 0.062	6.06AlaVal: 6.06 ± 0.085	0.595AlaTrp: 0.595 ± 0.026	2.743AlaTyr: 2.743 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	1.055CysAla: 1.055 ± 0.03	0.32CysCys: 0.32 ± 0.018	0.811CysAsp: 0.811 ± 0.028	0.957CysGlu: 0.957 ± 0.032	0.654CysPhe: 0.654 ± 0.022	1.47CysGly: 1.47 ± 0.044	0.326CysHis: 0.326 ± 0.02	1.176CysIle: 1.176 ± 0.036	0.895CysLys: 0.895 ± 0.029	1.18CysLeu: 1.18 ± 0.035	0.509CysMet: 0.509 ± 0.025	0.606CysAsn: 0.606 ± 0.025	0.649CysPro: 0.649 ± 0.028	0.462CysGln: 0.462 ± 0.022	0.717CysArg: 0.717 ± 0.028	0.963CysSer: 0.963 ± 0.034	0.788CysThr: 0.788 ± 0.025	1.032CysVal: 1.032 ± 0.028	0.165CysTrp: 0.165 ± 0.013	0.598CysTyr: 0.598 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	4.092AspAla: 4.092 ± 0.074	0.845AspCys: 0.845 ± 0.027	2.992AspAsp: 2.992 ± 0.081	4.711AspGlu: 4.711 ± 0.083	2.615AspPhe: 2.615 ± 0.057	4.443AspGly: 4.443 ± 0.081	0.875AspHis: 0.875 ± 0.031	4.398AspIle: 4.398 ± 0.07	3.653AspLys: 3.653 ± 0.066	4.511AspLeu: 4.511 ± 0.084	1.91AspMet: 1.91 ± 0.044	2.383AspAsn: 2.383 ± 0.058	1.761AspPro: 1.761 ± 0.054	1.527AspGln: 1.527 ± 0.043	2.402AspArg: 2.402 ± 0.048	3.378AspSer: 3.378 ± 0.07	3.356AspThr: 3.356 ± 0.064	3.744AspVal: 3.744 ± 0.061	0.584AspTrp: 0.584 ± 0.025	2.88AspTyr: 2.88 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	5.432GluAla: 5.432 ± 0.082	0.878GluCys: 0.878 ± 0.025	4.397GluAsp: 4.397 ± 0.081	7.48GluGlu: 7.48 ± 0.104	2.787GluPhe: 2.787 ± 0.052	4.217GluGly: 4.217 ± 0.069	1.413GluHis: 1.413 ± 0.04	5.773GluIle: 5.773 ± 0.072	7.424GluLys: 7.424 ± 0.095	6.901GluLeu: 6.901 ± 0.091	2.535GluMet: 2.535 ± 0.046	4.706GluAsn: 4.706 ± 0.069	1.868GluPro: 1.868 ± 0.048	3.055GluGln: 3.055 ± 0.061	3.306GluArg: 3.306 ± 0.059	3.447GluSer: 3.447 ± 0.065	4.031GluThr: 4.031 ± 0.056	4.362GluVal: 4.362 ± 0.064	0.675GluTrp: 0.675 ± 0.023	3.231GluTyr: 3.231 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	2.868PheAla: 2.868 ± 0.049	0.805PheCys: 0.805 ± 0.028	2.407PheAsp: 2.407 ± 0.052	2.56PheGlu: 2.56 ± 0.051	1.802PhePhe: 1.802 ± 0.051	2.886PheGly: 2.886 ± 0.052	0.882PheHis: 0.882 ± 0.026	2.604PheIle: 2.604 ± 0.049	2.022PheLys: 2.022 ± 0.042	3.917PheLeu: 3.917 ± 0.079	1.186PheMet: 1.186 ± 0.034	1.479PheAsn: 1.479 ± 0.038	1.378PhePro: 1.378 ± 0.037	1.429PheGln: 1.429 ± 0.036	1.735PheArg: 1.735 ± 0.043	2.993PheSer: 2.993 ± 0.055	2.341PheThr: 2.341 ± 0.049	2.682PheVal: 2.682 ± 0.056	0.47PheTrp: 0.47 ± 0.02	1.791PheTyr: 1.791 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	4.533GlyAla: 4.533 ± 0.09	1.298GlyCys: 1.298 ± 0.04	3.46GlyAsp: 3.46 ± 0.066	4.679GlyGlu: 4.679 ± 0.07	2.901GlyPhe: 2.901 ± 0.065	4.497GlyGly: 4.497 ± 0.1	1.257GlyHis: 1.257 ± 0.043	6.131GlyIle: 6.131 ± 0.08	5.947GlyLys: 5.947 ± 0.089	5.455GlyLeu: 5.455 ± 0.083	2.463GlyMet: 2.463 ± 0.056	3.357GlyAsn: 3.357 ± 0.056	1.114GlyPro: 1.114 ± 0.038	2.091GlyGln: 2.091 ± 0.044	2.871GlyArg: 2.871 ± 0.056	3.954GlySer: 3.954 ± 0.064	4.377GlyThr: 4.377 ± 0.075	4.639GlyVal: 4.639 ± 0.072	0.702GlyTrp: 0.702 ± 0.029	3.284GlyTyr: 3.284 ± 0.063	0.0GlyXaa: 0.0 ± 0.0
His	1.076HisAla: 1.076 ± 0.03	0.314HisCys: 0.314 ± 0.018	0.918HisAsp: 0.918 ± 0.033	1.171HisGlu: 1.171 ± 0.037	0.829HisPhe: 0.829 ± 0.028	1.263HisGly: 1.263 ± 0.037	0.425HisHis: 0.425 ± 0.028	1.399HisIle: 1.399 ± 0.038	1.063HisLys: 1.063 ± 0.032	1.531HisLeu: 1.531 ± 0.045	0.628HisMet: 0.628 ± 0.026	0.727HisAsn: 0.727 ± 0.025	0.862HisPro: 0.862 ± 0.029	0.589HisGln: 0.589 ± 0.026	0.819HisArg: 0.819 ± 0.03	1.009HisSer: 1.009 ± 0.035	1.04HisThr: 1.04 ± 0.032	1.078HisVal: 1.078 ± 0.032	0.181HisTrp: 0.181 ± 0.013	0.8HisTyr: 0.8 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.232IleAla: 5.232 ± 0.079	1.369IleCys: 1.369 ± 0.036	3.842IleAsp: 3.842 ± 0.066	4.586IleGlu: 4.586 ± 0.076	3.006IlePhe: 3.006 ± 0.061	4.743IleGly: 4.743 ± 0.068	1.427IleHis: 1.427 ± 0.038	4.783IleIle: 4.783 ± 0.08	4.136IleLys: 4.136 ± 0.056	7.137IleLeu: 7.137 ± 0.087	2.028IleMet: 2.028 ± 0.043	2.836IleAsn: 2.836 ± 0.066	3.101IlePro: 3.101 ± 0.056	2.57IleGln: 2.57 ± 0.045	3.656IleArg: 3.656 ± 0.061	5.058IleSer: 5.058 ± 0.079	4.121IleThr: 4.121 ± 0.07	4.619IleVal: 4.619 ± 0.075	0.67IleTrp: 0.67 ± 0.03	2.87IleTyr: 2.87 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	5.118LysAla: 5.118 ± 0.077	0.881LysCys: 0.881 ± 0.035	4.061LysAsp: 4.061 ± 0.072	6.973LysGlu: 6.973 ± 0.101	2.118LysPhe: 2.118 ± 0.044	4.261LysGly: 4.261 ± 0.059	1.091LysHis: 1.091 ± 0.035	5.143LysIle: 5.143 ± 0.058	6.924LysLys: 6.924 ± 0.097	5.447LysLeu: 5.447 ± 0.076	2.486LysMet: 2.486 ± 0.053	3.974LysAsn: 3.974 ± 0.07	2.032LysPro: 2.032 ± 0.042	2.524LysGln: 2.524 ± 0.057	3.137LysArg: 3.137 ± 0.058	3.548LysSer: 3.548 ± 0.064	4.236LysThr: 4.236 ± 0.07	4.451LysVal: 4.451 ± 0.07	0.665LysTrp: 0.665 ± 0.027	3.155LysTyr: 3.155 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	6.441LeuAla: 6.441 ± 0.085	1.455LeuCys: 1.455 ± 0.041	5.049LeuAsp: 5.049 ± 0.073	6.327LeuGlu: 6.327 ± 0.09	3.558LeuPhe: 3.558 ± 0.067	5.737LeuGly: 5.737 ± 0.081	1.628LeuHis: 1.628 ± 0.043	5.96LeuIle: 5.96 ± 0.085	6.457LeuLys: 6.457 ± 0.086	8.302LeuLeu: 8.302 ± 0.121	2.591LeuMet: 2.591 ± 0.05	3.976LeuAsn: 3.976 ± 0.066	3.349LeuPro: 3.349 ± 0.06	2.921LeuGln: 2.921 ± 0.057	3.589LeuArg: 3.589 ± 0.061	5.987LeuSer: 5.987 ± 0.082	5.078LeuThr: 5.078 ± 0.069	5.207LeuVal: 5.207 ± 0.072	0.742LeuTrp: 0.742 ± 0.03	3.261LeuTyr: 3.261 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.403MetAla: 2.403 ± 0.047	0.393MetCys: 0.393 ± 0.02	1.923MetAsp: 1.923 ± 0.039	2.751MetGlu: 2.751 ± 0.05	1.064MetPhe: 1.064 ± 0.031	2.067MetGly: 2.067 ± 0.046	0.433MetHis: 0.433 ± 0.02	2.265MetIle: 2.265 ± 0.05	2.696MetLys: 2.696 ± 0.046	2.764MetLeu: 2.764 ± 0.051	0.948MetMet: 0.948 ± 0.032	1.65MetAsn: 1.65 ± 0.035	1.123MetPro: 1.123 ± 0.028	1.188MetGln: 1.188 ± 0.036	1.243MetArg: 1.243 ± 0.035	1.807MetSer: 1.807 ± 0.036	1.759MetThr: 1.759 ± 0.042	1.82MetVal: 1.82 ± 0.043	0.232MetTrp: 0.232 ± 0.014	0.915MetTyr: 0.915 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.16AsnAla: 3.16 ± 0.05	0.669AsnCys: 0.669 ± 0.026	2.225AsnAsp: 2.225 ± 0.052	2.904AsnGlu: 2.904 ± 0.055	1.653AsnPhe: 1.653 ± 0.039	3.705AsnGly: 3.705 ± 0.065	0.876AsnHis: 0.876 ± 0.028	3.363AsnIle: 3.363 ± 0.059	2.725AsnLys: 2.725 ± 0.063	3.998AsnLeu: 3.998 ± 0.062	1.402AsnMet: 1.402 ± 0.039	1.864AsnAsn: 1.864 ± 0.054	2.093AsnPro: 2.093 ± 0.042	1.548AsnGln: 1.548 ± 0.043	2.137AsnArg: 2.137 ± 0.053	2.514AsnSer: 2.514 ± 0.048	2.596AsnThr: 2.596 ± 0.046	2.899AsnVal: 2.899 ± 0.058	0.427AsnTrp: 0.427 ± 0.021	1.949AsnTyr: 1.949 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.348ProAla: 2.348 ± 0.052	0.403ProCys: 0.403 ± 0.021	2.438ProAsp: 2.438 ± 0.056	3.389ProGlu: 3.389 ± 0.062	1.518ProPhe: 1.518 ± 0.04	2.278ProGly: 2.278 ± 0.048	0.521ProHis: 0.521 ± 0.023	1.855ProIle: 1.855 ± 0.041	1.982ProLys: 1.982 ± 0.054	2.586ProLeu: 2.586 ± 0.046	0.853ProMet: 0.853 ± 0.029	1.158ProAsn: 1.158 ± 0.035	0.696ProPro: 0.696 ± 0.027	1.114ProGln: 1.114 ± 0.035	0.983ProArg: 0.983 ± 0.028	1.705ProSer: 1.705 ± 0.039	1.628ProThr: 1.628 ± 0.051	3.026ProVal: 3.026 ± 0.06	0.319ProTrp: 0.319 ± 0.015	1.463ProTyr: 1.463 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.479GlnAla: 2.479 ± 0.046	0.372GlnCys: 0.372 ± 0.019	1.752GlnAsp: 1.752 ± 0.044	3.144GlnGlu: 3.144 ± 0.057	1.186GlnPhe: 1.186 ± 0.027	2.047GlnGly: 2.047 ± 0.042	0.512GlnHis: 0.512 ± 0.021	2.766GlnIle: 2.766 ± 0.056	3.208GlnLys: 3.208 ± 0.063	2.791GlnLeu: 2.791 ± 0.047	1.285GlnMet: 1.285 ± 0.036	1.851GlnAsn: 1.851 ± 0.045	0.993GlnPro: 0.993 ± 0.032	1.351GlnGln: 1.351 ± 0.041	1.43GlnArg: 1.43 ± 0.042	1.758GlnSer: 1.758 ± 0.04	1.872GlnThr: 1.872 ± 0.046	2.095GlnVal: 2.095 ± 0.045	0.319GlnTrp: 0.319 ± 0.02	1.446GlnTyr: 1.446 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.494ArgAla: 2.494 ± 0.05	0.618ArgCys: 0.618 ± 0.025	2.136ArgAsp: 2.136 ± 0.048	3.792ArgGlu: 3.792 ± 0.068	1.782ArgPhe: 1.782 ± 0.04	2.308ArgGly: 2.308 ± 0.049	0.801ArgHis: 0.801 ± 0.029	3.372ArgIle: 3.372 ± 0.059	3.914ArgLys: 3.914 ± 0.065	3.757ArgLeu: 3.757 ± 0.063	1.558ArgMet: 1.558 ± 0.045	2.201ArgAsn: 2.201 ± 0.048	1.257ArgPro: 1.257 ± 0.039	1.785ArgGln: 1.785 ± 0.044	2.234ArgArg: 2.234 ± 0.056	2.112ArgSer: 2.112 ± 0.045	2.203ArgThr: 2.203 ± 0.045	2.448ArgVal: 2.448 ± 0.045	0.376ArgTrp: 0.376 ± 0.019	1.94ArgTyr: 1.94 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	4.19SerAla: 4.19 ± 0.073	0.863SerCys: 0.863 ± 0.033	3.518SerAsp: 3.518 ± 0.06	4.169SerGlu: 4.169 ± 0.068	2.538SerPhe: 2.538 ± 0.054	4.933SerGly: 4.933 ± 0.076	1.017SerHis: 1.017 ± 0.031	3.9SerIle: 3.9 ± 0.062	3.351SerLys: 3.351 ± 0.056	5.033SerLeu: 5.033 ± 0.071	1.801SerMet: 1.801 ± 0.042	2.29SerAsn: 2.29 ± 0.055	1.633SerPro: 1.633 ± 0.038	1.993SerGln: 1.993 ± 0.043	2.746SerArg: 2.746 ± 0.044	3.727SerSer: 3.727 ± 0.073	2.882SerThr: 2.882 ± 0.058	4.552SerVal: 4.552 ± 0.066	0.595SerTrp: 0.595 ± 0.024	2.675SerTyr: 2.675 ± 0.064	0.0SerXaa: 0.0 ± 0.0
Thr	4.509ThrAla: 4.509 ± 0.075	0.701ThrCys: 0.701 ± 0.025	3.623ThrAsp: 3.623 ± 0.071	4.266ThrGlu: 4.266 ± 0.064	2.225ThrPhe: 2.225 ± 0.047	4.758ThrGly: 4.758 ± 0.074	0.887ThrHis: 0.887 ± 0.029	3.907ThrIle: 3.907 ± 0.066	3.082ThrLys: 3.082 ± 0.059	4.739ThrLeu: 4.739 ± 0.066	1.444ThrMet: 1.444 ± 0.034	1.978ThrAsn: 1.978 ± 0.043	2.251ThrPro: 2.251 ± 0.064	1.729ThrGln: 1.729 ± 0.045	2.038ThrArg: 2.038 ± 0.048	3.064ThrSer: 3.064 ± 0.059	2.89ThrThr: 2.89 ± 0.059	4.778ThrVal: 4.778 ± 0.084	0.525ThrTrp: 0.525 ± 0.022	2.136ThrTyr: 2.136 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	4.563ValAla: 4.563 ± 0.075	1.252ValCys: 1.252 ± 0.039	3.684ValAsp: 3.684 ± 0.062	4.467ValGlu: 4.467 ± 0.06	2.904ValPhe: 2.904 ± 0.052	3.975ValGly: 3.975 ± 0.061	1.128ValHis: 1.128 ± 0.034	4.954ValIle: 4.954 ± 0.069	4.701ValLys: 4.701 ± 0.071	6.474ValLeu: 6.474 ± 0.087	2.041ValMet: 2.041 ± 0.048	2.881ValAsn: 2.881 ± 0.053	2.449ValPro: 2.449 ± 0.05	2.186ValGln: 2.186 ± 0.042	2.799ValArg: 2.799 ± 0.054	4.641ValSer: 4.641 ± 0.078	4.225ValThr: 4.225 ± 0.081	4.558ValVal: 4.558 ± 0.07	0.648ValTrp: 0.648 ± 0.025	2.72ValTyr: 2.72 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.521TrpAla: 0.521 ± 0.024	0.142TrpCys: 0.142 ± 0.012	0.528TrpAsp: 0.528 ± 0.023	0.74TrpGlu: 0.74 ± 0.029	0.397TrpPhe: 0.397 ± 0.02	0.616TrpGly: 0.616 ± 0.024	0.184TrpHis: 0.184 ± 0.014	0.663TrpIle: 0.663 ± 0.025	0.877TrpLys: 0.877 ± 0.034	0.873TrpLeu: 0.873 ± 0.033	0.35TrpMet: 0.35 ± 0.017	0.589TrpAsn: 0.589 ± 0.023	0.206TrpPro: 0.206 ± 0.013	0.399TrpGln: 0.399 ± 0.019	0.356TrpArg: 0.356 ± 0.018	0.525TrpSer: 0.525 ± 0.024	0.436TrpThr: 0.436 ± 0.02	0.506TrpVal: 0.506 ± 0.021	0.098TrpTrp: 0.098 ± 0.009	0.378TrpTyr: 0.378 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.716TyrAla: 2.716 ± 0.052	0.681TyrCys: 0.681 ± 0.026	2.585TyrAsp: 2.585 ± 0.056	3.21TyrGlu: 3.21 ± 0.057	1.861TyrPhe: 1.861 ± 0.045	3.002TyrGly: 3.002 ± 0.052	0.942TyrHis: 0.942 ± 0.028	2.771TyrIle: 2.771 ± 0.053	2.432TyrLys: 2.432 ± 0.051	3.942TyrLeu: 3.942 ± 0.07	1.111TyrMet: 1.111 ± 0.036	1.796TyrAsn: 1.796 ± 0.039	1.474TyrPro: 1.474 ± 0.037	1.692TyrGln: 1.692 ± 0.039	2.038TyrArg: 2.038 ± 0.047	2.429TyrSer: 2.429 ± 0.049	2.465TyrThr: 2.465 ± 0.052	2.691TyrVal: 2.691 ± 0.052	0.397TyrTrp: 0.397 ± 0.019	2.063TyrTyr: 2.063 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3566 proteins (1070409 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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