Escherichia phage vB_EcoM_Goslar

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Goslarvirus; Escherichia virus Goslar

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482GG04|A0A482GG04_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_Goslar OX=2502409 GN=Goslar_00124 PE=4 SV=1
MM1 pKa = 7.81SDD3 pKa = 3.03TVTTIDD9 pKa = 3.76DD10 pKa = 3.94TEE12 pKa = 4.79LYY14 pKa = 9.49PVDD17 pKa = 3.66VSLPSSDD24 pKa = 4.5LVYY27 pKa = 11.3GLINHH32 pKa = 7.23DD33 pKa = 4.13NPPQNGEE40 pKa = 4.03HH41 pKa = 6.16VLTPEE46 pKa = 4.4LLVLGTPTTNQSAYY60 pKa = 10.16EE61 pKa = 4.21RR62 pKa = 11.84DD63 pKa = 3.71TQITATATAKK73 pKa = 9.41LTEE76 pKa = 4.37LDD78 pKa = 4.31DD79 pKa = 4.46YY80 pKa = 11.48EE81 pKa = 6.09GSTTLYY87 pKa = 9.98YY88 pKa = 10.94DD89 pKa = 4.75RR90 pKa = 11.84INIGAYY96 pKa = 9.22LRR98 pKa = 11.84NVAPILIDD106 pKa = 4.16APDD109 pKa = 3.62ATKK112 pKa = 10.07MRR114 pKa = 11.84DD115 pKa = 2.43IWPIIKK121 pKa = 9.92EE122 pKa = 4.04RR123 pKa = 11.84FGLGLTAEE131 pKa = 4.67EE132 pKa = 4.38IVNDD136 pKa = 3.8EE137 pKa = 4.73LNWDD141 pKa = 4.01TVNGQLLRR149 pKa = 11.84VVSSCLAWTGSVRR162 pKa = 11.84ILMKK166 pKa = 10.67SRR168 pKa = 11.84VPNLDD173 pKa = 4.13DD174 pKa = 4.64IIAIKK179 pKa = 10.36EE180 pKa = 3.76LDD182 pKa = 3.4GLKK185 pKa = 10.62FFGQGVRR192 pKa = 11.84ASGEE196 pKa = 3.88IYY198 pKa = 10.27SYY200 pKa = 11.63SRR202 pKa = 11.84DD203 pKa = 3.38TSDD206 pKa = 3.42YY207 pKa = 11.13GEE209 pKa = 4.68LLASLVTGTVISDD222 pKa = 3.54VVVADD227 pKa = 4.94LLAEE231 pKa = 4.12MTADD235 pKa = 3.7RR236 pKa = 11.84WVLDD240 pKa = 4.61DD241 pKa = 4.93NAAPYY246 pKa = 10.61NLYY249 pKa = 9.37NAKK252 pKa = 10.15VAFNGEE258 pKa = 3.85LGGRR262 pKa = 11.84IPARR266 pKa = 11.84AGYY269 pKa = 10.03QYY271 pKa = 10.84GIEE274 pKa = 4.11IGLDD278 pKa = 3.33EE279 pKa = 4.9NYY281 pKa = 8.33CTAVGGPLIFYY292 pKa = 9.85YY293 pKa = 10.8NVDD296 pKa = 2.89GHH298 pKa = 5.52TASLQGRR305 pKa = 11.84SPEE308 pKa = 4.13EE309 pKa = 3.65QGG311 pKa = 3.64

Molecular weight: 34.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482GN54|A0A482GN54_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_Goslar OX=2502409 GN=Goslar_00162 PE=4 SV=1
MM1 pKa = 7.61ILQMLGCLYY10 pKa = 10.78LSGWLIEE17 pKa = 4.31TVTVIIGLHH26 pKa = 4.32KK27 pKa = 10.31HH28 pKa = 6.16GIKK31 pKa = 10.46SPTYY35 pKa = 8.5TLPAHH40 pKa = 7.19CIAMMGAFPFIALGSIFWPFYY61 pKa = 7.89WFEE64 pKa = 3.78RR65 pKa = 11.84RR66 pKa = 11.84LPEE69 pKa = 3.8EE70 pKa = 3.9VRR72 pKa = 11.84QKK74 pKa = 10.63RR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 3.61ALARR82 pKa = 11.84RR83 pKa = 11.84EE84 pKa = 3.58EE85 pKa = 3.99RR86 pKa = 11.84QRR88 pKa = 11.84NKK90 pKa = 10.44GKK92 pKa = 10.62RR93 pKa = 11.84KK94 pKa = 9.58

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

247

0

247

73520

31

2252

297.7

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.739 ± 0.195

0.948 ± 0.055

6.683 ± 0.097

6.352 ± 0.152

3.675 ± 0.072

5.898 ± 0.22

1.991 ± 0.087

6.039 ± 0.12

5.207 ± 0.134

8.887 ± 0.128

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.712 ± 0.103

4.999 ± 0.125

4.221 ± 0.096

3.814 ± 0.101

5.994 ± 0.136

5.713 ± 0.113

6.62 ± 0.171

6.911 ± 0.137

1.411 ± 0.057

4.187 ± 0.106
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski