Amino acid dipepetide frequency for Bifidobacterium phage BitterVaud1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.16AlaAla: 7.16 ± 0.969
0.498AlaCys: 0.498 ± 0.201
5.915AlaAsp: 5.915 ± 0.454
4.234AlaGlu: 4.234 ± 0.389
3.175AlaPhe: 3.175 ± 0.971
4.794AlaGly: 4.794 ± 1.309
1.868AlaHis: 1.868 ± 0.416
5.043AlaIle: 5.043 ± 0.642
5.355AlaLys: 5.355 ± 0.519
6.039AlaLeu: 6.039 ± 1.138
2.491AlaMet: 2.491 ± 0.536
3.3AlaAsn: 3.3 ± 0.474
2.491AlaPro: 2.491 ± 0.514
2.241AlaGln: 2.241 ± 0.351
3.549AlaArg: 3.549 ± 0.548
5.853AlaSer: 5.853 ± 0.866
3.985AlaThr: 3.985 ± 0.613
4.981AlaVal: 4.981 ± 0.652
1.183AlaTrp: 1.183 ± 0.357
2.428AlaTyr: 2.428 ± 0.515
0.0AlaXaa: 0.0 ± 0.0
Cys
0.311CysAla: 0.311 ± 0.151
0.062CysCys: 0.062 ± 0.061
0.809CysAsp: 0.809 ± 0.295
0.623CysGlu: 0.623 ± 0.22
0.436CysPhe: 0.436 ± 0.151
0.685CysGly: 0.685 ± 0.281
0.374CysHis: 0.374 ± 0.147
0.747CysIle: 0.747 ± 0.223
0.374CysLys: 0.374 ± 0.17
0.747CysLeu: 0.747 ± 0.219
0.062CysMet: 0.062 ± 0.098
0.747CysAsn: 0.747 ± 0.219
0.436CysPro: 0.436 ± 0.204
0.498CysGln: 0.498 ± 0.23
0.436CysArg: 0.436 ± 0.206
0.809CysSer: 0.809 ± 0.255
0.249CysThr: 0.249 ± 0.109
0.56CysVal: 0.56 ± 0.233
0.062CysTrp: 0.062 ± 0.059
0.187CysTyr: 0.187 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
5.479AspAla: 5.479 ± 0.597
0.498AspCys: 0.498 ± 0.217
6.102AspAsp: 6.102 ± 0.987
4.919AspGlu: 4.919 ± 0.788
2.677AspPhe: 2.677 ± 0.414
5.417AspGly: 5.417 ± 0.825
2.055AspHis: 2.055 ± 0.35
5.977AspIle: 5.977 ± 0.692
5.043AspLys: 5.043 ± 0.801
6.164AspLeu: 6.164 ± 0.663
1.557AspMet: 1.557 ± 0.26
2.926AspAsn: 2.926 ± 0.543
3.798AspPro: 3.798 ± 0.615
3.113AspGln: 3.113 ± 0.442
4.047AspArg: 4.047 ± 0.595
5.479AspSer: 5.479 ± 0.678
3.798AspThr: 3.798 ± 0.579
4.421AspVal: 4.421 ± 0.763
0.996AspTrp: 0.996 ± 0.205
2.615AspTyr: 2.615 ± 0.472
0.0AspXaa: 0.0 ± 0.0
Glu
3.362GluAla: 3.362 ± 0.398
0.872GluCys: 0.872 ± 0.332
4.856GluAsp: 4.856 ± 0.658
3.362GluGlu: 3.362 ± 0.536
2.428GluPhe: 2.428 ± 0.472
2.926GluGly: 2.926 ± 0.385
1.93GluHis: 1.93 ± 0.305
3.798GluIle: 3.798 ± 0.442
3.238GluLys: 3.238 ± 0.6
4.545GluLeu: 4.545 ± 0.497
2.055GluMet: 2.055 ± 0.343
3.362GluAsn: 3.362 ± 0.484
2.055GluPro: 2.055 ± 0.331
2.304GluGln: 2.304 ± 0.401
3.549GluArg: 3.549 ± 0.783
3.673GluSer: 3.673 ± 0.527
2.428GluThr: 2.428 ± 0.301
4.109GluVal: 4.109 ± 0.51
0.996GluTrp: 0.996 ± 0.184
2.179GluTyr: 2.179 ± 0.417
0.0GluXaa: 0.0 ± 0.0
Phe
2.989PheAla: 2.989 ± 0.497
0.498PheCys: 0.498 ± 0.199
2.864PheAsp: 2.864 ± 0.533
2.179PheGlu: 2.179 ± 0.366
0.56PhePhe: 0.56 ± 0.221
3.3PheGly: 3.3 ± 0.411
0.996PheHis: 0.996 ± 0.224
1.619PheIle: 1.619 ± 0.327
1.308PheLys: 1.308 ± 0.241
1.992PheLeu: 1.992 ± 0.316
1.121PheMet: 1.121 ± 0.278
2.304PheAsn: 2.304 ± 0.325
1.245PhePro: 1.245 ± 0.279
1.494PheGln: 1.494 ± 0.298
2.055PheArg: 2.055 ± 0.318
3.051PheSer: 3.051 ± 0.544
1.743PheThr: 1.743 ± 0.381
1.868PheVal: 1.868 ± 0.276
0.56PheTrp: 0.56 ± 0.186
1.308PheTyr: 1.308 ± 0.31
0.0PheXaa: 0.0 ± 0.0
Gly
5.479GlyAla: 5.479 ± 1.271
1.183GlyCys: 1.183 ± 0.387
5.168GlyAsp: 5.168 ± 0.72
3.487GlyGlu: 3.487 ± 0.513
2.677GlyPhe: 2.677 ± 0.422
4.483GlyGly: 4.483 ± 0.999
1.121GlyHis: 1.121 ± 0.272
5.355GlyIle: 5.355 ± 0.702
3.985GlyLys: 3.985 ± 0.475
7.222GlyLeu: 7.222 ± 2.185
2.802GlyMet: 2.802 ± 0.504
3.487GlyAsn: 3.487 ± 0.516
1.058GlyPro: 1.058 ± 0.256
2.304GlyGln: 2.304 ± 0.485
3.86GlyArg: 3.86 ± 0.41
5.479GlySer: 5.479 ± 1.053
3.736GlyThr: 3.736 ± 0.408
4.483GlyVal: 4.483 ± 0.658
1.183GlyTrp: 1.183 ± 0.327
2.802GlyTyr: 2.802 ± 0.483
0.0GlyXaa: 0.0 ± 0.0
His
1.494HisAla: 1.494 ± 0.365
0.311HisCys: 0.311 ± 0.142
1.557HisAsp: 1.557 ± 0.325
1.245HisGlu: 1.245 ± 0.33
0.747HisPhe: 0.747 ± 0.267
1.432HisGly: 1.432 ± 0.293
0.747HisHis: 0.747 ± 0.245
1.93HisIle: 1.93 ± 0.387
0.809HisLys: 0.809 ± 0.24
1.681HisLeu: 1.681 ± 0.27
0.623HisMet: 0.623 ± 0.216
0.872HisAsn: 0.872 ± 0.261
1.183HisPro: 1.183 ± 0.304
0.996HisGln: 0.996 ± 0.221
1.183HisArg: 1.183 ± 0.29
1.743HisSer: 1.743 ± 0.412
0.996HisThr: 0.996 ± 0.24
1.308HisVal: 1.308 ± 0.331
0.374HisTrp: 0.374 ± 0.19
0.934HisTyr: 0.934 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
5.043IleAla: 5.043 ± 0.618
0.498IleCys: 0.498 ± 0.253
5.292IleAsp: 5.292 ± 0.677
3.3IleGlu: 3.3 ± 0.498
3.362IlePhe: 3.362 ± 0.424
4.234IleGly: 4.234 ± 0.798
0.809IleHis: 0.809 ± 0.269
3.673IleIle: 3.673 ± 0.537
4.296IleLys: 4.296 ± 0.481
4.919IleLeu: 4.919 ± 0.684
1.494IleMet: 1.494 ± 0.277
2.802IleAsn: 2.802 ± 0.459
3.549IlePro: 3.549 ± 0.454
2.615IleGln: 2.615 ± 0.468
3.923IleArg: 3.923 ± 0.494
5.479IleSer: 5.479 ± 0.728
3.113IleThr: 3.113 ± 0.459
3.487IleVal: 3.487 ± 0.404
0.934IleTrp: 0.934 ± 0.202
1.494IleTyr: 1.494 ± 0.279
0.0IleXaa: 0.0 ± 0.0
Lys
4.483LysAla: 4.483 ± 0.53
0.498LysCys: 0.498 ± 0.198
4.172LysAsp: 4.172 ± 0.645
3.424LysGlu: 3.424 ± 0.429
1.806LysPhe: 1.806 ± 0.316
3.362LysGly: 3.362 ± 0.523
1.308LysHis: 1.308 ± 0.32
3.051LysIle: 3.051 ± 0.448
3.985LysLys: 3.985 ± 0.552
5.168LysLeu: 5.168 ± 0.627
1.743LysMet: 1.743 ± 0.295
3.424LysAsn: 3.424 ± 0.466
2.241LysPro: 2.241 ± 0.447
2.677LysGln: 2.677 ± 0.469
2.179LysArg: 2.179 ± 0.425
4.234LysSer: 4.234 ± 0.442
4.296LysThr: 4.296 ± 0.492
4.421LysVal: 4.421 ± 0.573
1.245LysTrp: 1.245 ± 0.329
2.677LysTyr: 2.677 ± 0.368
0.0LysXaa: 0.0 ± 0.0
Leu
7.409LeuAla: 7.409 ± 1.672
0.374LeuCys: 0.374 ± 0.146
6.226LeuAsp: 6.226 ± 0.562
4.607LeuGlu: 4.607 ± 0.552
2.304LeuPhe: 2.304 ± 0.42
6.538LeuGly: 6.538 ± 1.402
1.868LeuHis: 1.868 ± 0.417
4.607LeuIle: 4.607 ± 0.441
5.168LeuLys: 5.168 ± 0.674
3.736LeuLeu: 3.736 ± 0.378
2.304LeuMet: 2.304 ± 0.311
3.611LeuAsn: 3.611 ± 0.463
2.428LeuPro: 2.428 ± 0.391
2.74LeuGln: 2.74 ± 0.375
5.106LeuArg: 5.106 ± 0.523
5.977LeuSer: 5.977 ± 0.824
3.985LeuThr: 3.985 ± 0.522
4.732LeuVal: 4.732 ± 0.578
0.498LeuTrp: 0.498 ± 0.136
2.615LeuTyr: 2.615 ± 0.447
0.0LeuXaa: 0.0 ± 0.0
Met
2.802MetAla: 2.802 ± 0.48
0.249MetCys: 0.249 ± 0.122
1.619MetAsp: 1.619 ± 0.313
1.308MetGlu: 1.308 ± 0.236
1.058MetPhe: 1.058 ± 0.24
2.117MetGly: 2.117 ± 0.561
0.498MetHis: 0.498 ± 0.187
1.245MetIle: 1.245 ± 0.303
1.992MetLys: 1.992 ± 0.371
2.491MetLeu: 2.491 ± 0.321
0.56MetMet: 0.56 ± 0.18
1.557MetAsn: 1.557 ± 0.331
0.934MetPro: 0.934 ± 0.197
0.809MetGln: 0.809 ± 0.196
1.308MetArg: 1.308 ± 0.283
2.677MetSer: 2.677 ± 0.417
1.743MetThr: 1.743 ± 0.303
1.619MetVal: 1.619 ± 0.27
0.249MetTrp: 0.249 ± 0.137
0.809MetTyr: 0.809 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
3.549AsnAla: 3.549 ± 0.487
0.249AsnCys: 0.249 ± 0.125
4.856AsnAsp: 4.856 ± 0.699
2.802AsnGlu: 2.802 ± 0.531
1.432AsnPhe: 1.432 ± 0.302
4.607AsnGly: 4.607 ± 0.768
1.494AsnHis: 1.494 ± 0.353
2.241AsnIle: 2.241 ± 0.389
2.428AsnLys: 2.428 ± 0.549
4.296AsnLeu: 4.296 ± 0.769
1.121AsnMet: 1.121 ± 0.2
2.304AsnAsn: 2.304 ± 0.341
2.926AsnPro: 2.926 ± 0.417
1.93AsnGln: 1.93 ± 0.337
3.611AsnArg: 3.611 ± 0.569
3.673AsnSer: 3.673 ± 0.482
2.677AsnThr: 2.677 ± 0.41
2.615AsnVal: 2.615 ± 0.303
0.374AsnTrp: 0.374 ± 0.162
1.183AsnTyr: 1.183 ± 0.259
0.0AsnXaa: 0.0 ± 0.0
Pro
2.864ProAla: 2.864 ± 0.606
0.436ProCys: 0.436 ± 0.16
3.673ProAsp: 3.673 ± 0.667
3.673ProGlu: 3.673 ± 0.608
1.058ProPhe: 1.058 ± 0.229
3.113ProGly: 3.113 ± 0.431
0.934ProHis: 0.934 ± 0.313
2.241ProIle: 2.241 ± 0.431
2.179ProLys: 2.179 ± 0.5
2.117ProLeu: 2.117 ± 0.324
0.996ProMet: 0.996 ± 0.22
1.619ProAsn: 1.619 ± 0.349
1.183ProPro: 1.183 ± 0.211
0.747ProGln: 0.747 ± 0.26
1.992ProArg: 1.992 ± 0.389
2.428ProSer: 2.428 ± 0.39
1.992ProThr: 1.992 ± 0.464
2.989ProVal: 2.989 ± 0.497
0.809ProTrp: 0.809 ± 0.222
1.743ProTyr: 1.743 ± 0.302
0.0ProXaa: 0.0 ± 0.0
Gln
2.553GlnAla: 2.553 ± 0.382
0.249GlnCys: 0.249 ± 0.159
2.055GlnAsp: 2.055 ± 0.433
2.677GlnGlu: 2.677 ± 0.509
1.37GlnPhe: 1.37 ± 0.316
2.615GlnGly: 2.615 ± 0.545
0.685GlnHis: 0.685 ± 0.266
1.992GlnIle: 1.992 ± 0.342
2.491GlnLys: 2.491 ± 0.338
3.362GlnLeu: 3.362 ± 0.549
1.245GlnMet: 1.245 ± 0.283
1.93GlnAsn: 1.93 ± 0.391
1.245GlnPro: 1.245 ± 0.229
1.308GlnGln: 1.308 ± 0.284
1.93GlnArg: 1.93 ± 0.374
2.553GlnSer: 2.553 ± 0.391
2.428GlnThr: 2.428 ± 0.355
2.366GlnVal: 2.366 ± 0.458
0.498GlnTrp: 0.498 ± 0.183
1.432GlnTyr: 1.432 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
3.549ArgAla: 3.549 ± 0.516
0.685ArgCys: 0.685 ± 0.21
3.86ArgAsp: 3.86 ± 0.465
3.175ArgGlu: 3.175 ± 0.538
2.179ArgPhe: 2.179 ± 0.397
2.74ArgGly: 2.74 ± 0.371
1.37ArgHis: 1.37 ± 0.319
3.736ArgIle: 3.736 ± 0.551
4.172ArgLys: 4.172 ± 0.678
3.798ArgLeu: 3.798 ± 0.575
1.37ArgMet: 1.37 ± 0.294
3.549ArgAsn: 3.549 ± 0.549
1.992ArgPro: 1.992 ± 0.451
1.868ArgGln: 1.868 ± 0.298
3.673ArgArg: 3.673 ± 0.607
3.673ArgSer: 3.673 ± 0.445
3.487ArgThr: 3.487 ± 0.522
2.926ArgVal: 2.926 ± 0.477
0.809ArgTrp: 0.809 ± 0.237
1.806ArgTyr: 1.806 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
5.728SerAla: 5.728 ± 0.931
0.56SerCys: 0.56 ± 0.231
5.292SerAsp: 5.292 ± 0.777
4.296SerGlu: 4.296 ± 0.5
1.992SerPhe: 1.992 ± 0.368
6.413SerGly: 6.413 ± 1.349
0.996SerHis: 0.996 ± 0.263
5.168SerIle: 5.168 ± 0.788
3.985SerLys: 3.985 ± 0.526
5.728SerLeu: 5.728 ± 0.845
2.553SerMet: 2.553 ± 0.381
3.487SerAsn: 3.487 ± 0.547
2.74SerPro: 2.74 ± 0.449
2.677SerGln: 2.677 ± 0.37
3.3SerArg: 3.3 ± 0.44
5.666SerSer: 5.666 ± 1.153
3.238SerThr: 3.238 ± 0.422
6.102SerVal: 6.102 ± 0.962
1.058SerTrp: 1.058 ± 0.284
2.864SerTyr: 2.864 ± 0.481
0.0SerXaa: 0.0 ± 0.0
Thr
4.358ThrAla: 4.358 ± 0.672
0.249ThrCys: 0.249 ± 0.111
3.923ThrAsp: 3.923 ± 0.369
2.553ThrGlu: 2.553 ± 0.415
2.179ThrPhe: 2.179 ± 0.396
5.106ThrGly: 5.106 ± 0.533
0.934ThrHis: 0.934 ± 0.285
4.172ThrIle: 4.172 ± 0.503
3.611ThrLys: 3.611 ± 0.477
4.047ThrLeu: 4.047 ± 0.533
1.183ThrMet: 1.183 ± 0.233
2.553ThrAsn: 2.553 ± 0.472
2.304ThrPro: 2.304 ± 0.409
2.055ThrGln: 2.055 ± 0.411
2.241ThrArg: 2.241 ± 0.349
3.238ThrSer: 3.238 ± 0.342
2.802ThrThr: 2.802 ± 0.494
3.86ThrVal: 3.86 ± 0.543
0.809ThrTrp: 0.809 ± 0.252
1.868ThrTyr: 1.868 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
4.109ValAla: 4.109 ± 0.685
0.685ValCys: 0.685 ± 0.254
4.296ValAsp: 4.296 ± 0.661
3.3ValGlu: 3.3 ± 0.503
1.681ValPhe: 1.681 ± 0.351
3.798ValGly: 3.798 ± 0.681
1.557ValHis: 1.557 ± 0.331
4.421ValIle: 4.421 ± 0.557
3.113ValLys: 3.113 ± 0.452
5.292ValLeu: 5.292 ± 0.513
1.619ValMet: 1.619 ± 0.287
3.673ValAsn: 3.673 ± 0.538
2.864ValPro: 2.864 ± 0.529
1.992ValGln: 1.992 ± 0.438
4.047ValArg: 4.047 ± 0.509
5.043ValSer: 5.043 ± 0.741
4.109ValThr: 4.109 ± 0.473
4.047ValVal: 4.047 ± 0.553
0.623ValTrp: 0.623 ± 0.23
2.989ValTyr: 2.989 ± 0.516
0.0ValXaa: 0.0 ± 0.0
Trp
1.058TrpAla: 1.058 ± 0.22
0.187TrpCys: 0.187 ± 0.108
0.872TrpAsp: 0.872 ± 0.228
0.747TrpGlu: 0.747 ± 0.223
0.374TrpPhe: 0.374 ± 0.178
0.872TrpGly: 0.872 ± 0.195
0.311TrpHis: 0.311 ± 0.186
0.996TrpIle: 0.996 ± 0.3
1.058TrpLys: 1.058 ± 0.282
0.872TrpLeu: 0.872 ± 0.198
0.249TrpMet: 0.249 ± 0.123
1.308TrpAsn: 1.308 ± 0.317
0.374TrpPro: 0.374 ± 0.184
0.809TrpGln: 0.809 ± 0.228
0.623TrpArg: 0.623 ± 0.176
1.058TrpSer: 1.058 ± 0.288
0.934TrpThr: 0.934 ± 0.226
0.498TrpVal: 0.498 ± 0.272
0.062TrpTrp: 0.062 ± 0.073
0.436TrpTyr: 0.436 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.802TyrAla: 2.802 ± 0.464
0.436TyrCys: 0.436 ± 0.204
3.673TyrAsp: 3.673 ± 0.698
2.117TyrGlu: 2.117 ± 0.444
1.557TyrPhe: 1.557 ± 0.405
2.802TyrGly: 2.802 ± 0.54
0.311TyrHis: 0.311 ± 0.137
2.304TyrIle: 2.304 ± 0.404
1.806TyrLys: 1.806 ± 0.388
2.615TyrLeu: 2.615 ± 0.496
0.436TyrMet: 0.436 ± 0.224
1.557TyrAsn: 1.557 ± 0.302
1.743TyrPro: 1.743 ± 0.402
1.806TyrGln: 1.806 ± 0.355
1.806TyrArg: 1.806 ± 0.399
2.055TyrSer: 2.055 ± 0.36
2.304TyrThr: 2.304 ± 0.304
1.868TyrVal: 1.868 ± 0.421
0.374TyrTrp: 0.374 ± 0.171
1.557TyrTyr: 1.557 ± 0.415
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (16062 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski