Amino acid dipepetide frequency for Paenibacillus phage Vegas

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.525AlaAla: 3.525 ± 0.639
0.484AlaCys: 0.484 ± 0.226
4.423AlaAsp: 4.423 ± 0.61
6.773AlaGlu: 6.773 ± 0.749
2.142AlaPhe: 2.142 ± 0.489
5.183AlaGly: 5.183 ± 0.66
1.382AlaHis: 1.382 ± 0.335
4.838AlaIle: 4.838 ± 0.718
5.114AlaLys: 5.114 ± 0.743
6.842AlaLeu: 6.842 ± 1.116
2.142AlaMet: 2.142 ± 0.438
2.695AlaAsn: 2.695 ± 0.492
2.35AlaPro: 2.35 ± 0.395
2.281AlaGln: 2.281 ± 0.556
3.11AlaArg: 3.11 ± 0.414
4.423AlaSer: 4.423 ± 0.66
3.317AlaThr: 3.317 ± 0.557
4.492AlaVal: 4.492 ± 0.981
1.52AlaTrp: 1.52 ± 0.347
1.935AlaTyr: 1.935 ± 0.445
0.0AlaXaa: 0.0 ± 0.0
Cys
0.415CysAla: 0.415 ± 0.18
0.0CysCys: 0.0 ± 0.0
0.415CysAsp: 0.415 ± 0.185
0.484CysGlu: 0.484 ± 0.206
0.276CysPhe: 0.276 ± 0.141
1.106CysGly: 1.106 ± 0.341
0.415CysHis: 0.415 ± 0.163
0.415CysIle: 0.415 ± 0.152
1.106CysLys: 1.106 ± 0.308
0.691CysLeu: 0.691 ± 0.222
0.207CysMet: 0.207 ± 0.122
0.622CysAsn: 0.622 ± 0.239
0.484CysPro: 0.484 ± 0.223
0.415CysGln: 0.415 ± 0.182
0.415CysArg: 0.415 ± 0.178
0.553CysSer: 0.553 ± 0.201
0.276CysThr: 0.276 ± 0.137
0.691CysVal: 0.691 ± 0.243
0.207CysTrp: 0.207 ± 0.113
0.415CysTyr: 0.415 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
3.663AspAla: 3.663 ± 0.682
0.553AspCys: 0.553 ± 0.216
3.663AspAsp: 3.663 ± 0.58
5.529AspGlu: 5.529 ± 0.682
2.557AspPhe: 2.557 ± 0.409
4.147AspGly: 4.147 ± 0.598
1.106AspHis: 1.106 ± 0.321
2.833AspIle: 2.833 ± 0.428
3.732AspLys: 3.732 ± 0.649
4.285AspLeu: 4.285 ± 0.544
1.728AspMet: 1.728 ± 0.316
2.211AspAsn: 2.211 ± 0.34
2.073AspPro: 2.073 ± 0.396
2.211AspGln: 2.211 ± 0.508
2.695AspArg: 2.695 ± 0.577
2.833AspSer: 2.833 ± 0.395
3.041AspThr: 3.041 ± 0.393
3.87AspVal: 3.87 ± 0.539
1.451AspTrp: 1.451 ± 0.318
2.142AspTyr: 2.142 ± 0.418
0.0AspXaa: 0.0 ± 0.0
Glu
6.565GluAla: 6.565 ± 0.722
0.691GluCys: 0.691 ± 0.179
4.423GluAsp: 4.423 ± 0.695
6.22GluGlu: 6.22 ± 0.878
3.248GluPhe: 3.248 ± 0.476
3.87GluGly: 3.87 ± 0.596
1.728GluHis: 1.728 ± 0.385
6.151GluIle: 6.151 ± 0.565
6.842GluLys: 6.842 ± 0.847
7.947GluLeu: 7.947 ± 0.74
3.248GluMet: 3.248 ± 0.617
3.801GluAsn: 3.801 ± 0.583
2.211GluPro: 2.211 ± 0.426
4.147GluGln: 4.147 ± 0.616
4.561GluArg: 4.561 ± 0.596
4.285GluSer: 4.285 ± 0.748
3.455GluThr: 3.455 ± 0.456
4.976GluVal: 4.976 ± 0.568
1.728GluTrp: 1.728 ± 0.55
2.833GluTyr: 2.833 ± 0.492
0.0GluXaa: 0.0 ± 0.0
Phe
1.935PheAla: 1.935 ± 0.629
0.898PheCys: 0.898 ± 0.239
2.972PheAsp: 2.972 ± 0.56
3.11PheGlu: 3.11 ± 0.511
1.106PhePhe: 1.106 ± 0.263
3.041PheGly: 3.041 ± 0.429
0.968PheHis: 0.968 ± 0.334
2.626PheIle: 2.626 ± 0.481
2.695PheLys: 2.695 ± 0.4
2.695PheLeu: 2.695 ± 0.452
0.898PheMet: 0.898 ± 0.269
2.419PheAsn: 2.419 ± 0.435
1.382PhePro: 1.382 ± 0.357
1.659PheGln: 1.659 ± 0.419
1.175PheArg: 1.175 ± 0.277
2.488PheSer: 2.488 ± 0.407
2.073PheThr: 2.073 ± 0.438
2.488PheVal: 2.488 ± 0.552
0.415PheTrp: 0.415 ± 0.189
1.52PheTyr: 1.52 ± 0.321
0.0PheXaa: 0.0 ± 0.0
Gly
3.87GlyAla: 3.87 ± 0.681
0.691GlyCys: 0.691 ± 0.192
4.561GlyAsp: 4.561 ± 0.565
5.667GlyGlu: 5.667 ± 0.72
2.211GlyPhe: 2.211 ± 0.395
4.354GlyGly: 4.354 ± 0.6
0.76GlyHis: 0.76 ± 0.28
4.423GlyIle: 4.423 ± 1.015
5.943GlyLys: 5.943 ± 0.848
4.768GlyLeu: 4.768 ± 0.765
2.073GlyMet: 2.073 ± 0.468
3.248GlyAsn: 3.248 ± 0.578
0.829GlyPro: 0.829 ± 0.201
2.073GlyGln: 2.073 ± 0.554
2.972GlyArg: 2.972 ± 0.564
3.732GlySer: 3.732 ± 0.481
2.972GlyThr: 2.972 ± 0.435
5.39GlyVal: 5.39 ± 0.616
1.451GlyTrp: 1.451 ± 0.387
2.626GlyTyr: 2.626 ± 0.491
0.0GlyXaa: 0.0 ± 0.0
His
1.037HisAla: 1.037 ± 0.262
0.346HisCys: 0.346 ± 0.191
0.898HisAsp: 0.898 ± 0.235
1.175HisGlu: 1.175 ± 0.268
1.313HisPhe: 1.313 ± 0.277
0.968HisGly: 0.968 ± 0.338
0.968HisHis: 0.968 ± 0.319
1.451HisIle: 1.451 ± 0.332
1.106HisLys: 1.106 ± 0.337
1.935HisLeu: 1.935 ± 0.439
0.553HisMet: 0.553 ± 0.183
0.829HisAsn: 0.829 ± 0.264
0.691HisPro: 0.691 ± 0.191
0.76HisGln: 0.76 ± 0.233
0.968HisArg: 0.968 ± 0.228
1.244HisSer: 1.244 ± 0.315
0.898HisThr: 0.898 ± 0.24
1.451HisVal: 1.451 ± 0.285
0.207HisTrp: 0.207 ± 0.12
0.346HisTyr: 0.346 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
5.183IleAla: 5.183 ± 0.919
0.898IleCys: 0.898 ± 0.268
4.285IleAsp: 4.285 ± 0.636
6.289IleGlu: 6.289 ± 0.715
2.073IlePhe: 2.073 ± 0.378
4.216IleGly: 4.216 ± 0.79
1.589IleHis: 1.589 ± 0.386
3.801IleIle: 3.801 ± 0.719
5.598IleLys: 5.598 ± 0.766
4.63IleLeu: 4.63 ± 0.67
1.659IleMet: 1.659 ± 0.503
2.764IleAsn: 2.764 ± 0.39
2.764IlePro: 2.764 ± 0.501
3.317IleGln: 3.317 ± 0.571
3.455IleArg: 3.455 ± 0.403
3.317IleSer: 3.317 ± 0.504
3.11IleThr: 3.11 ± 0.354
3.87IleVal: 3.87 ± 0.495
0.553IleTrp: 0.553 ± 0.178
1.52IleTyr: 1.52 ± 0.368
0.0IleXaa: 0.0 ± 0.0
Lys
6.151LysAla: 6.151 ± 0.664
0.415LysCys: 0.415 ± 0.209
3.248LysAsp: 3.248 ± 0.461
6.634LysGlu: 6.634 ± 0.796
2.833LysPhe: 2.833 ± 0.385
5.598LysGly: 5.598 ± 0.67
1.244LysHis: 1.244 ± 0.304
3.732LysIle: 3.732 ± 0.502
5.736LysLys: 5.736 ± 0.86
8.017LysLeu: 8.017 ± 0.711
2.695LysMet: 2.695 ± 0.408
4.354LysAsn: 4.354 ± 0.574
2.488LysPro: 2.488 ± 0.412
3.732LysGln: 3.732 ± 0.574
5.321LysArg: 5.321 ± 0.63
4.354LysSer: 4.354 ± 0.479
4.699LysThr: 4.699 ± 0.565
4.285LysVal: 4.285 ± 0.545
0.829LysTrp: 0.829 ± 0.205
2.833LysTyr: 2.833 ± 0.487
0.0LysXaa: 0.0 ± 0.0
Leu
7.187LeuAla: 7.187 ± 0.833
1.175LeuCys: 1.175 ± 0.32
4.838LeuAsp: 4.838 ± 0.688
7.395LeuGlu: 7.395 ± 0.72
3.041LeuPhe: 3.041 ± 0.475
5.183LeuGly: 5.183 ± 0.86
1.728LeuHis: 1.728 ± 0.337
5.045LeuIle: 5.045 ± 0.522
7.74LeuLys: 7.74 ± 0.786
6.082LeuLeu: 6.082 ± 0.898
1.866LeuMet: 1.866 ± 0.374
4.077LeuAsn: 4.077 ± 0.491
3.525LeuPro: 3.525 ± 0.409
3.317LeuGln: 3.317 ± 0.497
5.114LeuArg: 5.114 ± 0.709
6.151LeuSer: 6.151 ± 0.516
4.561LeuThr: 4.561 ± 0.608
4.63LeuVal: 4.63 ± 0.559
0.76LeuTrp: 0.76 ± 0.232
2.626LeuTyr: 2.626 ± 0.433
0.0LeuXaa: 0.0 ± 0.0
Met
1.797MetAla: 1.797 ± 0.387
0.207MetCys: 0.207 ± 0.13
1.313MetAsp: 1.313 ± 0.297
2.695MetGlu: 2.695 ± 0.387
1.106MetPhe: 1.106 ± 0.324
2.281MetGly: 2.281 ± 0.405
0.346MetHis: 0.346 ± 0.161
2.211MetIle: 2.211 ± 0.515
2.903MetLys: 2.903 ± 0.428
2.35MetLeu: 2.35 ± 0.383
0.829MetMet: 0.829 ± 0.257
1.659MetAsn: 1.659 ± 0.343
1.313MetPro: 1.313 ± 0.325
1.313MetGln: 1.313 ± 0.331
1.797MetArg: 1.797 ± 0.387
2.004MetSer: 2.004 ± 0.331
1.659MetThr: 1.659 ± 0.339
1.313MetVal: 1.313 ± 0.346
0.415MetTrp: 0.415 ± 0.167
0.76MetTyr: 0.76 ± 0.275
0.0MetXaa: 0.0 ± 0.0
Asn
3.317AsnAla: 3.317 ± 0.737
0.415AsnCys: 0.415 ± 0.145
1.659AsnAsp: 1.659 ± 0.362
2.557AsnGlu: 2.557 ± 0.459
1.037AsnPhe: 1.037 ± 0.266
4.354AsnGly: 4.354 ± 0.483
0.553AsnHis: 0.553 ± 0.222
2.764AsnIle: 2.764 ± 0.484
3.11AsnLys: 3.11 ± 0.448
4.423AsnLeu: 4.423 ± 0.527
1.866AsnMet: 1.866 ± 0.456
1.659AsnAsn: 1.659 ± 0.333
2.419AsnPro: 2.419 ± 0.486
2.833AsnGln: 2.833 ± 0.472
2.972AsnArg: 2.972 ± 0.471
2.419AsnSer: 2.419 ± 0.408
1.935AsnThr: 1.935 ± 0.365
2.903AsnVal: 2.903 ± 0.471
0.829AsnTrp: 0.829 ± 0.28
1.797AsnTyr: 1.797 ± 0.354
0.0AsnXaa: 0.0 ± 0.0
Pro
2.488ProAla: 2.488 ± 0.449
0.207ProCys: 0.207 ± 0.109
2.419ProAsp: 2.419 ± 0.518
3.11ProGlu: 3.11 ± 0.498
1.175ProPhe: 1.175 ± 0.312
1.728ProGly: 1.728 ± 0.325
0.898ProHis: 0.898 ± 0.269
2.557ProIle: 2.557 ± 0.407
3.041ProLys: 3.041 ± 0.565
2.903ProLeu: 2.903 ± 0.553
0.622ProMet: 0.622 ± 0.228
1.935ProAsn: 1.935 ± 0.298
1.728ProPro: 1.728 ± 0.361
1.106ProGln: 1.106 ± 0.397
1.589ProArg: 1.589 ± 0.377
2.419ProSer: 2.419 ± 0.456
2.004ProThr: 2.004 ± 0.378
2.419ProVal: 2.419 ± 0.542
0.207ProTrp: 0.207 ± 0.129
0.968ProTyr: 0.968 ± 0.262
0.0ProXaa: 0.0 ± 0.0
Gln
4.216GlnAla: 4.216 ± 0.621
0.346GlnCys: 0.346 ± 0.168
2.142GlnAsp: 2.142 ± 0.392
2.903GlnGlu: 2.903 ± 0.542
2.004GlnPhe: 2.004 ± 0.376
2.211GlnGly: 2.211 ± 0.365
0.691GlnHis: 0.691 ± 0.211
2.903GlnIle: 2.903 ± 0.445
3.386GlnLys: 3.386 ± 0.463
4.354GlnLeu: 4.354 ± 0.554
2.281GlnMet: 2.281 ± 0.445
1.589GlnAsn: 1.589 ± 0.295
1.382GlnPro: 1.382 ± 0.333
2.35GlnGln: 2.35 ± 0.665
2.073GlnArg: 2.073 ± 0.389
1.797GlnSer: 1.797 ± 0.462
2.281GlnThr: 2.281 ± 0.367
2.972GlnVal: 2.972 ± 0.548
0.415GlnTrp: 0.415 ± 0.171
1.313GlnTyr: 1.313 ± 0.357
0.0GlnXaa: 0.0 ± 0.0
Arg
2.142ArgAla: 2.142 ± 0.333
0.276ArgCys: 0.276 ± 0.141
2.557ArgAsp: 2.557 ± 0.373
4.838ArgGlu: 4.838 ± 0.632
2.419ArgPhe: 2.419 ± 0.435
2.764ArgGly: 2.764 ± 0.596
1.313ArgHis: 1.313 ± 0.328
3.801ArgIle: 3.801 ± 0.523
5.252ArgLys: 5.252 ± 0.672
4.838ArgLeu: 4.838 ± 0.705
1.313ArgMet: 1.313 ± 0.266
2.419ArgAsn: 2.419 ± 0.42
1.797ArgPro: 1.797 ± 0.375
2.833ArgGln: 2.833 ± 0.441
2.764ArgArg: 2.764 ± 0.427
2.972ArgSer: 2.972 ± 0.497
2.073ArgThr: 2.073 ± 0.336
2.972ArgVal: 2.972 ± 0.617
0.898ArgTrp: 0.898 ± 0.299
2.281ArgTyr: 2.281 ± 0.477
0.0ArgXaa: 0.0 ± 0.0
Ser
4.838SerAla: 4.838 ± 0.686
0.622SerCys: 0.622 ± 0.211
2.695SerAsp: 2.695 ± 0.448
4.077SerGlu: 4.077 ± 0.711
2.695SerPhe: 2.695 ± 0.495
3.732SerGly: 3.732 ± 0.51
1.037SerHis: 1.037 ± 0.269
4.354SerIle: 4.354 ± 0.441
4.423SerLys: 4.423 ± 0.535
4.699SerLeu: 4.699 ± 0.592
1.728SerMet: 1.728 ± 0.316
2.695SerAsn: 2.695 ± 0.519
2.004SerPro: 2.004 ± 0.34
2.419SerGln: 2.419 ± 0.499
2.903SerArg: 2.903 ± 0.487
3.317SerSer: 3.317 ± 0.599
3.041SerThr: 3.041 ± 0.45
4.077SerVal: 4.077 ± 0.542
0.622SerTrp: 0.622 ± 0.196
2.281SerTyr: 2.281 ± 0.433
0.0SerXaa: 0.0 ± 0.0
Thr
3.732ThrAla: 3.732 ± 0.451
0.207ThrCys: 0.207 ± 0.115
2.35ThrAsp: 2.35 ± 0.389
4.077ThrGlu: 4.077 ± 0.683
1.866ThrPhe: 1.866 ± 0.334
3.525ThrGly: 3.525 ± 0.433
0.484ThrHis: 0.484 ± 0.224
2.972ThrIle: 2.972 ± 0.444
3.248ThrLys: 3.248 ± 0.438
4.216ThrLeu: 4.216 ± 0.516
1.037ThrMet: 1.037 ± 0.328
2.419ThrAsn: 2.419 ± 0.424
2.833ThrPro: 2.833 ± 0.563
1.866ThrGln: 1.866 ± 0.371
2.073ThrArg: 2.073 ± 0.455
2.695ThrSer: 2.695 ± 0.37
2.903ThrThr: 2.903 ± 0.498
4.077ThrVal: 4.077 ± 0.612
0.76ThrTrp: 0.76 ± 0.243
2.073ThrTyr: 2.073 ± 0.412
0.0ThrXaa: 0.0 ± 0.0
Val
3.87ValAla: 3.87 ± 0.608
0.622ValCys: 0.622 ± 0.204
3.732ValAsp: 3.732 ± 0.517
5.183ValGlu: 5.183 ± 0.727
3.248ValPhe: 3.248 ± 0.483
3.386ValGly: 3.386 ± 0.531
1.175ValHis: 1.175 ± 0.308
4.976ValIle: 4.976 ± 0.898
5.114ValLys: 5.114 ± 0.598
6.012ValLeu: 6.012 ± 0.665
1.797ValMet: 1.797 ± 0.425
2.004ValAsn: 2.004 ± 0.334
2.211ValPro: 2.211 ± 0.323
2.419ValGln: 2.419 ± 0.427
3.939ValArg: 3.939 ± 0.557
4.699ValSer: 4.699 ± 0.69
3.386ValThr: 3.386 ± 0.49
5.252ValVal: 5.252 ± 0.597
0.898ValTrp: 0.898 ± 0.236
1.451ValTyr: 1.451 ± 0.371
0.0ValXaa: 0.0 ± 0.0
Trp
0.968TrpAla: 0.968 ± 0.242
0.207TrpCys: 0.207 ± 0.116
1.313TrpAsp: 1.313 ± 0.315
0.76TrpGlu: 0.76 ± 0.242
0.76TrpPhe: 0.76 ± 0.251
0.968TrpGly: 0.968 ± 0.274
0.276TrpHis: 0.276 ± 0.141
1.313TrpIle: 1.313 ± 0.443
1.106TrpLys: 1.106 ± 0.286
1.313TrpLeu: 1.313 ± 0.289
0.553TrpMet: 0.553 ± 0.178
0.76TrpAsn: 0.76 ± 0.239
0.138TrpPro: 0.138 ± 0.094
0.622TrpGln: 0.622 ± 0.213
0.829TrpArg: 0.829 ± 0.308
1.106TrpSer: 1.106 ± 0.247
0.276TrpThr: 0.276 ± 0.13
0.691TrpVal: 0.691 ± 0.228
0.276TrpTrp: 0.276 ± 0.149
0.622TrpTyr: 0.622 ± 0.23
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.073TyrAla: 2.073 ± 0.396
0.346TyrCys: 0.346 ± 0.153
2.419TyrAsp: 2.419 ± 0.477
3.455TyrGlu: 3.455 ± 0.455
1.451TyrPhe: 1.451 ± 0.318
2.004TyrGly: 2.004 ± 0.428
0.484TyrHis: 0.484 ± 0.22
1.728TyrIle: 1.728 ± 0.373
2.004TyrLys: 2.004 ± 0.354
3.041TyrLeu: 3.041 ± 0.55
1.106TyrMet: 1.106 ± 0.275
1.659TyrAsn: 1.659 ± 0.301
0.968TyrPro: 0.968 ± 0.253
1.935TyrGln: 1.935 ± 0.355
1.797TyrArg: 1.797 ± 0.405
1.451TyrSer: 1.451 ± 0.301
1.451TyrThr: 1.451 ± 0.351
2.557TyrVal: 2.557 ± 0.479
0.415TyrTrp: 0.415 ± 0.159
1.728TyrTyr: 1.728 ± 0.404
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (14471 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski