Amino acid dipepetide frequency for Synechococcus phage Syn30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.626AlaAla: 6.626 ± 0.431
0.515AlaCys: 0.515 ± 0.112
4.192AlaAsp: 4.192 ± 0.26
3.908AlaGlu: 3.908 ± 0.344
2.931AlaPhe: 2.931 ± 0.239
6.875AlaGly: 6.875 ± 0.567
0.924AlaHis: 0.924 ± 0.122
4.174AlaIle: 4.174 ± 0.31
3.748AlaLys: 3.748 ± 0.335
4.796AlaLeu: 4.796 ± 0.293
1.386AlaMet: 1.386 ± 0.199
3.961AlaAsn: 3.961 ± 0.421
2.824AlaPro: 2.824 ± 0.208
2.789AlaGln: 2.789 ± 0.207
2.753AlaArg: 2.753 ± 0.225
5.205AlaSer: 5.205 ± 0.398
6.235AlaThr: 6.235 ± 0.816
4.388AlaVal: 4.388 ± 0.367
0.639AlaTrp: 0.639 ± 0.135
2.185AlaTyr: 2.185 ± 0.148
0.0AlaXaa: 0.0 ± 0.0
Cys
0.462CysAla: 0.462 ± 0.092
0.071CysCys: 0.071 ± 0.035
0.657CysAsp: 0.657 ± 0.13
0.568CysGlu: 0.568 ± 0.114
0.373CysPhe: 0.373 ± 0.102
0.551CysGly: 0.551 ± 0.111
0.195CysHis: 0.195 ± 0.056
0.551CysIle: 0.551 ± 0.129
0.604CysLys: 0.604 ± 0.114
0.657CysLeu: 0.657 ± 0.12
0.302CysMet: 0.302 ± 0.085
0.48CysAsn: 0.48 ± 0.108
0.338CysPro: 0.338 ± 0.107
0.373CysGln: 0.373 ± 0.091
0.462CysArg: 0.462 ± 0.1
0.551CysSer: 0.551 ± 0.1
0.639CysThr: 0.639 ± 0.114
0.462CysVal: 0.462 ± 0.12
0.124CysTrp: 0.124 ± 0.05
0.338CysTyr: 0.338 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
5.169AspAla: 5.169 ± 0.344
0.533AspCys: 0.533 ± 0.122
4.583AspAsp: 4.583 ± 0.413
3.926AspGlu: 3.926 ± 0.355
2.967AspPhe: 2.967 ± 0.236
5.738AspGly: 5.738 ± 0.418
0.995AspHis: 0.995 ± 0.119
3.926AspIle: 3.926 ± 0.288
3.286AspLys: 3.286 ± 0.311
5.08AspLeu: 5.08 ± 0.404
1.474AspMet: 1.474 ± 0.216
3.464AspAsn: 3.464 ± 0.286
3.322AspPro: 3.322 ± 0.223
2.203AspGln: 2.203 ± 0.202
2.38AspArg: 2.38 ± 0.282
4.21AspSer: 4.21 ± 0.3
4.317AspThr: 4.317 ± 0.352
4.086AspVal: 4.086 ± 0.336
1.137AspTrp: 1.137 ± 0.138
3.197AspTyr: 3.197 ± 0.231
0.0AspXaa: 0.0 ± 0.0
Glu
3.126GluAla: 3.126 ± 0.276
0.746GluCys: 0.746 ± 0.127
4.032GluAsp: 4.032 ± 0.269
4.565GluGlu: 4.565 ± 0.524
3.197GluPhe: 3.197 ± 0.271
3.57GluGly: 3.57 ± 0.312
0.924GluHis: 0.924 ± 0.14
4.245GluIle: 4.245 ± 0.296
3.908GluLys: 3.908 ± 0.464
4.956GluLeu: 4.956 ± 0.401
1.439GluMet: 1.439 ± 0.199
2.984GluAsn: 2.984 ± 0.223
1.936GluPro: 1.936 ± 0.216
2.025GluGln: 2.025 ± 0.224
2.38GluArg: 2.38 ± 0.322
3.624GluSer: 3.624 ± 0.295
3.624GluThr: 3.624 ± 0.271
4.174GluVal: 4.174 ± 0.331
0.817GluTrp: 0.817 ± 0.13
3.002GluTyr: 3.002 ± 0.246
0.0GluXaa: 0.0 ± 0.0
Phe
2.878PheAla: 2.878 ± 0.23
0.444PheCys: 0.444 ± 0.115
3.553PheAsp: 3.553 ± 0.219
2.576PheGlu: 2.576 ± 0.247
1.776PhePhe: 1.776 ± 0.169
2.86PheGly: 2.86 ± 0.269
0.568PheHis: 0.568 ± 0.113
2.434PheIle: 2.434 ± 0.213
2.149PheLys: 2.149 ± 0.188
2.842PheLeu: 2.842 ± 0.287
1.03PheMet: 1.03 ± 0.169
2.895PheAsn: 2.895 ± 0.209
1.812PhePro: 1.812 ± 0.192
1.652PheGln: 1.652 ± 0.183
1.581PheArg: 1.581 ± 0.15
3.073PheSer: 3.073 ± 0.252
3.446PheThr: 3.446 ± 0.549
3.038PheVal: 3.038 ± 0.266
0.391PheTrp: 0.391 ± 0.078
1.883PheTyr: 1.883 ± 0.164
0.0PheXaa: 0.0 ± 0.0
Gly
6.448GlyAla: 6.448 ± 0.593
0.657GlyCys: 0.657 ± 0.117
5.205GlyAsp: 5.205 ± 0.64
4.317GlyGlu: 4.317 ± 0.27
3.002GlyPhe: 3.002 ± 0.22
7.834GlyGly: 7.834 ± 0.898
1.155GlyHis: 1.155 ± 0.172
4.37GlyIle: 4.37 ± 0.309
3.57GlyLys: 3.57 ± 0.33
4.849GlyLeu: 4.849 ± 0.302
1.403GlyMet: 1.403 ± 0.194
5.222GlyAsn: 5.222 ± 0.713
2.416GlyPro: 2.416 ± 0.246
2.647GlyGln: 2.647 ± 0.236
2.86GlyArg: 2.86 ± 0.254
6.857GlySer: 6.857 ± 0.565
6.786GlyThr: 6.786 ± 0.75
5.169GlyVal: 5.169 ± 0.389
0.906GlyTrp: 0.906 ± 0.131
3.304GlyTyr: 3.304 ± 0.286
0.0GlyXaa: 0.0 ± 0.0
His
0.728HisAla: 0.728 ± 0.115
0.249HisCys: 0.249 ± 0.067
0.782HisAsp: 0.782 ± 0.128
1.172HisGlu: 1.172 ± 0.143
0.782HisPhe: 0.782 ± 0.122
1.208HisGly: 1.208 ± 0.202
0.32HisHis: 0.32 ± 0.101
0.888HisIle: 0.888 ± 0.125
1.048HisLys: 1.048 ± 0.212
1.101HisLeu: 1.101 ± 0.161
0.426HisMet: 0.426 ± 0.101
0.906HisAsn: 0.906 ± 0.145
0.87HisPro: 0.87 ± 0.165
0.391HisGln: 0.391 ± 0.078
0.551HisArg: 0.551 ± 0.108
0.799HisSer: 0.799 ± 0.117
1.19HisThr: 1.19 ± 0.236
0.977HisVal: 0.977 ± 0.146
0.266HisTrp: 0.266 ± 0.074
0.817HisTyr: 0.817 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
4.388IleAla: 4.388 ± 0.304
0.48IleCys: 0.48 ± 0.117
5.063IleAsp: 5.063 ± 0.276
4.032IleGlu: 4.032 ± 0.329
2.522IlePhe: 2.522 ± 0.24
4.192IleGly: 4.192 ± 0.308
0.728IleHis: 0.728 ± 0.119
3.855IleIle: 3.855 ± 0.287
3.73IleLys: 3.73 ± 0.308
4.37IleLeu: 4.37 ± 0.312
1.048IleMet: 1.048 ± 0.161
4.068IleAsn: 4.068 ± 0.272
2.576IlePro: 2.576 ± 0.269
2.487IleGln: 2.487 ± 0.206
2.469IleArg: 2.469 ± 0.255
4.21IleSer: 4.21 ± 0.353
5.471IleThr: 5.471 ± 0.565
4.228IleVal: 4.228 ± 0.31
0.639IleTrp: 0.639 ± 0.108
2.238IleTyr: 2.238 ± 0.256
0.0IleXaa: 0.0 ± 0.0
Lys
3.464LysAla: 3.464 ± 0.334
0.675LysCys: 0.675 ± 0.116
3.713LysAsp: 3.713 ± 0.299
3.73LysGlu: 3.73 ± 0.457
2.522LysPhe: 2.522 ± 0.258
3.855LysGly: 3.855 ± 0.297
0.782LysHis: 0.782 ± 0.15
3.819LysIle: 3.819 ± 0.291
5.134LysLys: 5.134 ± 0.712
4.921LysLeu: 4.921 ± 0.402
1.439LysMet: 1.439 ± 0.196
3.251LysAsn: 3.251 ± 0.306
1.99LysPro: 1.99 ± 0.23
2.238LysGln: 2.238 ± 0.276
2.558LysArg: 2.558 ± 0.215
3.606LysSer: 3.606 ± 0.36
3.73LysThr: 3.73 ± 0.263
4.086LysVal: 4.086 ± 0.243
0.693LysTrp: 0.693 ± 0.115
2.895LysTyr: 2.895 ± 0.279
0.0LysXaa: 0.0 ± 0.0
Leu
4.405LeuAla: 4.405 ± 0.28
0.728LeuCys: 0.728 ± 0.137
5.294LeuAsp: 5.294 ± 0.352
4.441LeuGlu: 4.441 ± 0.322
2.54LeuPhe: 2.54 ± 0.248
5.063LeuGly: 5.063 ± 0.495
1.315LeuHis: 1.315 ± 0.235
3.926LeuIle: 3.926 ± 0.275
5.134LeuLys: 5.134 ± 0.369
5.098LeuLeu: 5.098 ± 0.428
1.403LeuMet: 1.403 ± 0.202
4.405LeuAsn: 4.405 ± 0.338
2.913LeuPro: 2.913 ± 0.253
2.771LeuGln: 2.771 ± 0.226
3.109LeuArg: 3.109 ± 0.241
5.258LeuSer: 5.258 ± 0.252
5.453LeuThr: 5.453 ± 0.499
4.405LeuVal: 4.405 ± 0.269
0.675LeuTrp: 0.675 ± 0.13
3.233LeuTyr: 3.233 ± 0.214
0.0LeuXaa: 0.0 ± 0.0
Met
1.492MetAla: 1.492 ± 0.205
0.124MetCys: 0.124 ± 0.046
1.19MetAsp: 1.19 ± 0.184
1.35MetGlu: 1.35 ± 0.245
1.119MetPhe: 1.119 ± 0.193
0.924MetGly: 0.924 ± 0.198
0.551MetHis: 0.551 ± 0.11
1.243MetIle: 1.243 ± 0.205
1.865MetLys: 1.865 ± 0.234
1.563MetLeu: 1.563 ± 0.211
0.515MetMet: 0.515 ± 0.115
1.226MetAsn: 1.226 ± 0.154
0.87MetPro: 0.87 ± 0.151
0.764MetGln: 0.764 ± 0.128
0.835MetArg: 0.835 ± 0.145
1.581MetSer: 1.581 ± 0.203
1.51MetThr: 1.51 ± 0.189
1.172MetVal: 1.172 ± 0.132
0.32MetTrp: 0.32 ± 0.089
0.551MetTyr: 0.551 ± 0.098
0.0MetXaa: 0.0 ± 0.0
Asn
4.423AsnAla: 4.423 ± 0.517
0.426AsnCys: 0.426 ± 0.105
3.126AsnAsp: 3.126 ± 0.268
2.878AsnGlu: 2.878 ± 0.218
2.469AsnPhe: 2.469 ± 0.177
4.53AsnGly: 4.53 ± 0.445
0.977AsnHis: 0.977 ± 0.122
3.73AsnIle: 3.73 ± 0.338
3.073AsnLys: 3.073 ± 0.258
4.654AsnLeu: 4.654 ± 0.445
1.084AsnMet: 1.084 ± 0.168
3.784AsnAsn: 3.784 ± 0.447
2.949AsnPro: 2.949 ± 0.279
2.558AsnGln: 2.558 ± 0.232
2.22AsnArg: 2.22 ± 0.215
4.21AsnSer: 4.21 ± 0.344
4.388AsnThr: 4.388 ± 0.535
5.027AsnVal: 5.027 ± 0.427
0.799AsnTrp: 0.799 ± 0.115
2.576AsnTyr: 2.576 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
2.842ProAla: 2.842 ± 0.26
0.249ProCys: 0.249 ± 0.089
2.807ProAsp: 2.807 ± 0.23
2.682ProGlu: 2.682 ± 0.301
1.705ProPhe: 1.705 ± 0.186
3.517ProGly: 3.517 ± 0.354
0.764ProHis: 0.764 ± 0.122
2.398ProIle: 2.398 ± 0.235
2.38ProLys: 2.38 ± 0.324
2.007ProLeu: 2.007 ± 0.192
0.817ProMet: 0.817 ± 0.149
2.416ProAsn: 2.416 ± 0.23
1.528ProPro: 1.528 ± 0.201
1.35ProGln: 1.35 ± 0.202
1.545ProArg: 1.545 ± 0.188
3.34ProSer: 3.34 ± 0.387
2.967ProThr: 2.967 ± 0.258
2.647ProVal: 2.647 ± 0.225
0.568ProTrp: 0.568 ± 0.116
1.723ProTyr: 1.723 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
2.363GlnAla: 2.363 ± 0.24
0.249GlnCys: 0.249 ± 0.065
1.918GlnAsp: 1.918 ± 0.15
2.398GlnGlu: 2.398 ± 0.262
1.599GlnPhe: 1.599 ± 0.189
2.256GlnGly: 2.256 ± 0.194
0.675GlnHis: 0.675 ± 0.132
2.629GlnIle: 2.629 ± 0.207
2.416GlnLys: 2.416 ± 0.257
3.251GlnLeu: 3.251 ± 0.257
0.711GlnMet: 0.711 ± 0.128
2.256GlnAsn: 2.256 ± 0.207
1.439GlnPro: 1.439 ± 0.178
1.581GlnGln: 1.581 ± 0.209
1.599GlnArg: 1.599 ± 0.157
2.238GlnSer: 2.238 ± 0.172
2.434GlnThr: 2.434 ± 0.222
2.611GlnVal: 2.611 ± 0.214
0.355GlnTrp: 0.355 ± 0.086
1.901GlnTyr: 1.901 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
2.434ArgAla: 2.434 ± 0.227
0.409ArgCys: 0.409 ± 0.087
2.043ArgAsp: 2.043 ± 0.182
2.274ArgGlu: 2.274 ± 0.293
1.688ArgPhe: 1.688 ± 0.15
2.913ArgGly: 2.913 ± 0.308
0.728ArgHis: 0.728 ± 0.125
2.984ArgIle: 2.984 ± 0.254
2.665ArgLys: 2.665 ± 0.319
2.895ArgLeu: 2.895 ± 0.222
1.155ArgMet: 1.155 ± 0.188
1.865ArgAsn: 1.865 ± 0.203
1.403ArgPro: 1.403 ± 0.156
1.652ArgGln: 1.652 ± 0.174
2.167ArgArg: 2.167 ± 0.359
2.345ArgSer: 2.345 ± 0.191
2.398ArgThr: 2.398 ± 0.25
2.931ArgVal: 2.931 ± 0.286
0.373ArgTrp: 0.373 ± 0.084
2.22ArgTyr: 2.22 ± 0.203
0.0ArgXaa: 0.0 ± 0.0
Ser
5.56SerAla: 5.56 ± 0.36
0.444SerCys: 0.444 ± 0.104
4.405SerAsp: 4.405 ± 0.321
3.375SerGlu: 3.375 ± 0.236
3.215SerPhe: 3.215 ± 0.311
7.176SerGly: 7.176 ± 0.583
0.941SerHis: 0.941 ± 0.134
4.921SerIle: 4.921 ± 0.386
3.553SerLys: 3.553 ± 0.258
4.547SerLeu: 4.547 ± 0.257
1.243SerMet: 1.243 ± 0.169
4.725SerAsn: 4.725 ± 0.434
2.753SerPro: 2.753 ± 0.354
2.451SerGln: 2.451 ± 0.225
2.22SerArg: 2.22 ± 0.205
5.596SerSer: 5.596 ± 0.491
5.613SerThr: 5.613 ± 0.537
4.619SerVal: 4.619 ± 0.375
0.693SerTrp: 0.693 ± 0.099
2.434SerTyr: 2.434 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
6.377ThrAla: 6.377 ± 0.601
0.444ThrCys: 0.444 ± 0.14
4.068ThrAsp: 4.068 ± 0.288
3.713ThrGlu: 3.713 ± 0.265
3.837ThrPhe: 3.837 ± 0.539
7.692ThrGly: 7.692 ± 1.115
1.03ThrHis: 1.03 ± 0.181
6.004ThrIle: 6.004 ± 0.49
3.695ThrLys: 3.695 ± 0.211
6.111ThrLeu: 6.111 ± 0.502
1.172ThrMet: 1.172 ± 0.184
4.423ThrAsn: 4.423 ± 0.572
3.197ThrPro: 3.197 ± 0.248
2.665ThrGln: 2.665 ± 0.208
2.611ThrArg: 2.611 ± 0.237
5.684ThrSer: 5.684 ± 0.68
6.875ThrThr: 6.875 ± 0.864
3.517ThrVal: 3.517 ± 0.441
0.853ThrTrp: 0.853 ± 0.101
2.753ThrTyr: 2.753 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
4.512ValAla: 4.512 ± 0.349
0.551ValCys: 0.551 ± 0.097
5.063ValAsp: 5.063 ± 0.318
4.103ValGlu: 4.103 ± 0.222
2.505ValPhe: 2.505 ± 0.234
5.063ValGly: 5.063 ± 0.49
0.888ValHis: 0.888 ± 0.127
3.624ValIle: 3.624 ± 0.258
3.837ValLys: 3.837 ± 0.245
4.174ValLeu: 4.174 ± 0.221
1.19ValMet: 1.19 ± 0.17
4.103ValAsn: 4.103 ± 0.286
3.091ValPro: 3.091 ± 0.259
2.345ValGln: 2.345 ± 0.2
2.576ValArg: 2.576 ± 0.205
4.992ValSer: 4.992 ± 0.308
5.862ValThr: 5.862 ± 0.586
4.547ValVal: 4.547 ± 0.403
0.639ValTrp: 0.639 ± 0.083
2.292ValTyr: 2.292 ± 0.23
0.0ValXaa: 0.0 ± 0.0
Trp
0.675TrpAla: 0.675 ± 0.115
0.195TrpCys: 0.195 ± 0.057
0.799TrpAsp: 0.799 ± 0.121
0.711TrpGlu: 0.711 ± 0.129
0.533TrpPhe: 0.533 ± 0.112
0.551TrpGly: 0.551 ± 0.091
0.338TrpHis: 0.338 ± 0.092
0.604TrpIle: 0.604 ± 0.099
0.977TrpLys: 0.977 ± 0.176
0.728TrpLeu: 0.728 ± 0.145
0.355TrpMet: 0.355 ± 0.074
0.728TrpAsn: 0.728 ± 0.117
0.249TrpPro: 0.249 ± 0.072
0.373TrpGln: 0.373 ± 0.077
0.551TrpArg: 0.551 ± 0.126
0.764TrpSer: 0.764 ± 0.109
0.87TrpThr: 0.87 ± 0.121
0.728TrpVal: 0.728 ± 0.114
0.231TrpTrp: 0.231 ± 0.064
0.497TrpTyr: 0.497 ± 0.093
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.682TyrAla: 2.682 ± 0.27
0.568TyrCys: 0.568 ± 0.102
3.499TyrAsp: 3.499 ± 0.298
2.558TyrGlu: 2.558 ± 0.297
1.616TyrPhe: 1.616 ± 0.188
2.611TyrGly: 2.611 ± 0.18
0.675TyrHis: 0.675 ± 0.113
2.469TyrIle: 2.469 ± 0.242
2.327TyrLys: 2.327 ± 0.266
2.967TyrLeu: 2.967 ± 0.245
1.155TyrMet: 1.155 ± 0.175
2.718TyrAsn: 2.718 ± 0.229
1.776TyrPro: 1.776 ± 0.192
1.563TyrGln: 1.563 ± 0.173
2.114TyrArg: 2.114 ± 0.255
2.345TyrSer: 2.345 ± 0.21
2.913TyrThr: 2.913 ± 0.366
3.144TyrVal: 3.144 ± 0.251
0.338TyrTrp: 0.338 ± 0.094
2.149TyrTyr: 2.149 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 209 proteins (56296 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski