Pea necrotic yellow dwarf alphasatellite 1

Taxonomy: Viruses; Alphasatellitidae; Nanoalphasatellitinae; Mivedwarsatellite

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9TPD3|V9TPD3_9VIRU Satellite replication initiator protein OS=Pea necrotic yellow dwarf alphasatellite 1 OX=1441797 GN=para-Rep1 PE=4 SV=1
MM1 pKa = 7.69ASCNWVFTRR10 pKa = 11.84NFSGDD15 pKa = 3.43TPLLSFDD22 pKa = 3.92EE23 pKa = 4.53RR24 pKa = 11.84VQYY27 pKa = 11.15ACWQHH32 pKa = 5.67EE33 pKa = 4.72KK34 pKa = 10.36VAHH37 pKa = 6.35DD38 pKa = 3.92HH39 pKa = 5.72LQGVIQLKK47 pKa = 10.17KK48 pKa = 8.13RR49 pKa = 11.84ARR51 pKa = 11.84MNTVKK56 pKa = 10.68ALIGGNPHH64 pKa = 6.81VEE66 pKa = 3.78MMKK69 pKa = 10.84AKK71 pKa = 10.04IEE73 pKa = 3.97EE74 pKa = 4.04ASAYY78 pKa = 7.3VQKK81 pKa = 10.9EE82 pKa = 4.06EE83 pKa = 4.78SRR85 pKa = 11.84VAGPWIYY92 pKa = 11.38GEE94 pKa = 4.03MLKK97 pKa = 10.6KK98 pKa = 10.7GSNKK102 pKa = 8.99RR103 pKa = 11.84SLMEE107 pKa = 4.37RR108 pKa = 11.84YY109 pKa = 9.5KK110 pKa = 10.72EE111 pKa = 4.04DD112 pKa = 3.98PEE114 pKa = 4.78LMEE117 pKa = 6.09LEE119 pKa = 4.86DD120 pKa = 3.81PGKK123 pKa = 10.53ARR125 pKa = 11.84RR126 pKa = 11.84CRR128 pKa = 11.84ARR130 pKa = 11.84IDD132 pKa = 3.27KK133 pKa = 10.3EE134 pKa = 4.17KK135 pKa = 10.44FVSEE139 pKa = 4.42FKK141 pKa = 11.23VEE143 pKa = 4.53DD144 pKa = 5.08DD145 pKa = 3.63EE146 pKa = 5.7QEE148 pKa = 4.06WKK150 pKa = 10.83SLLEE154 pKa = 4.04EE155 pKa = 4.43DD156 pKa = 4.45LKK158 pKa = 11.48KK159 pKa = 10.43EE160 pKa = 3.82ASPRR164 pKa = 11.84SIMWVYY170 pKa = 10.4GPHH173 pKa = 6.12GGEE176 pKa = 4.34GKK178 pKa = 5.93TTKK181 pKa = 10.45AKK183 pKa = 10.5EE184 pKa = 3.89LITRR188 pKa = 11.84GFFYY192 pKa = 10.71TRR194 pKa = 11.84GGKK197 pKa = 9.54KK198 pKa = 10.11DD199 pKa = 3.72DD200 pKa = 3.44VAYY203 pKa = 10.73SYY205 pKa = 11.22IEE207 pKa = 4.41DD208 pKa = 3.8PTRR211 pKa = 11.84HH212 pKa = 5.0VVFDD216 pKa = 3.98IPRR219 pKa = 11.84DD220 pKa = 3.6MQEE223 pKa = 3.69YY224 pKa = 10.2CNYY227 pKa = 10.91SLIEE231 pKa = 3.98MLKK234 pKa = 10.7DD235 pKa = 4.34RR236 pKa = 11.84IIISNKK242 pKa = 9.28YY243 pKa = 9.83EE244 pKa = 4.99PITNCQLDD252 pKa = 4.37NIHH255 pKa = 6.63VIVMANFLPDD265 pKa = 3.2VTKK268 pKa = 10.73ISNDD272 pKa = 3.5RR273 pKa = 11.84IKK275 pKa = 10.46IIYY278 pKa = 8.92CC279 pKa = 3.79

Molecular weight: 32.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9TPD3|V9TPD3_9VIRU Satellite replication initiator protein OS=Pea necrotic yellow dwarf alphasatellite 1 OX=1441797 GN=para-Rep1 PE=4 SV=1
MM1 pKa = 7.69ASCNWVFTRR10 pKa = 11.84NFSGDD15 pKa = 3.43TPLLSFDD22 pKa = 3.92EE23 pKa = 4.53RR24 pKa = 11.84VQYY27 pKa = 11.15ACWQHH32 pKa = 5.67EE33 pKa = 4.72KK34 pKa = 10.36VAHH37 pKa = 6.35DD38 pKa = 3.92HH39 pKa = 5.72LQGVIQLKK47 pKa = 10.17KK48 pKa = 8.13RR49 pKa = 11.84ARR51 pKa = 11.84MNTVKK56 pKa = 10.68ALIGGNPHH64 pKa = 6.81VEE66 pKa = 3.78MMKK69 pKa = 10.84AKK71 pKa = 10.04IEE73 pKa = 3.97EE74 pKa = 4.04ASAYY78 pKa = 7.3VQKK81 pKa = 10.9EE82 pKa = 4.06EE83 pKa = 4.78SRR85 pKa = 11.84VAGPWIYY92 pKa = 11.38GEE94 pKa = 4.03MLKK97 pKa = 10.6KK98 pKa = 10.7GSNKK102 pKa = 8.99RR103 pKa = 11.84SLMEE107 pKa = 4.37RR108 pKa = 11.84YY109 pKa = 9.5KK110 pKa = 10.72EE111 pKa = 4.04DD112 pKa = 3.98PEE114 pKa = 4.78LMEE117 pKa = 6.09LEE119 pKa = 4.86DD120 pKa = 3.81PGKK123 pKa = 10.53ARR125 pKa = 11.84RR126 pKa = 11.84CRR128 pKa = 11.84ARR130 pKa = 11.84IDD132 pKa = 3.27KK133 pKa = 10.3EE134 pKa = 4.17KK135 pKa = 10.44FVSEE139 pKa = 4.42FKK141 pKa = 11.23VEE143 pKa = 4.53DD144 pKa = 5.08DD145 pKa = 3.63EE146 pKa = 5.7QEE148 pKa = 4.06WKK150 pKa = 10.83SLLEE154 pKa = 4.04EE155 pKa = 4.43DD156 pKa = 4.45LKK158 pKa = 11.48KK159 pKa = 10.43EE160 pKa = 3.82ASPRR164 pKa = 11.84SIMWVYY170 pKa = 10.4GPHH173 pKa = 6.12GGEE176 pKa = 4.34GKK178 pKa = 5.93TTKK181 pKa = 10.45AKK183 pKa = 10.5EE184 pKa = 3.89LITRR188 pKa = 11.84GFFYY192 pKa = 10.71TRR194 pKa = 11.84GGKK197 pKa = 9.54KK198 pKa = 10.11DD199 pKa = 3.72DD200 pKa = 3.44VAYY203 pKa = 10.73SYY205 pKa = 11.22IEE207 pKa = 4.41DD208 pKa = 3.8PTRR211 pKa = 11.84HH212 pKa = 5.0VVFDD216 pKa = 3.98IPRR219 pKa = 11.84DD220 pKa = 3.6MQEE223 pKa = 3.69YY224 pKa = 10.2CNYY227 pKa = 10.91SLIEE231 pKa = 3.98MLKK234 pKa = 10.7DD235 pKa = 4.34RR236 pKa = 11.84IIISNKK242 pKa = 9.28YY243 pKa = 9.83EE244 pKa = 4.99PITNCQLDD252 pKa = 4.37NIHH255 pKa = 6.63VIVMANFLPDD265 pKa = 3.2VTKK268 pKa = 10.73ISNDD272 pKa = 3.5RR273 pKa = 11.84IKK275 pKa = 10.46IIYY278 pKa = 8.92CC279 pKa = 3.79

Molecular weight: 32.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

279

279

279

279.0

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.376 ± 0.0

2.151 ± 0.0

6.452 ± 0.0

9.677 ± 0.0

3.226 ± 0.0

5.376 ± 0.0

2.509 ± 0.0

7.168 ± 0.0

9.677 ± 0.0

6.093 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.943 ± 0.0

3.943 ± 0.0

3.943 ± 0.0

2.867 ± 0.0

6.452 ± 0.0

5.376 ± 0.0

3.584 ± 0.0

6.093 ± 0.0

1.792 ± 0.0

4.301 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski