Amino acid dipepetide frequency for Clostridium sp. OM02-18AC

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.996AlaAla: 8.996 ± 0.146
1.287AlaCys: 1.287 ± 0.04
4.928AlaAsp: 4.928 ± 0.081
6.192AlaGlu: 6.192 ± 0.094
3.281AlaPhe: 3.281 ± 0.067
6.992AlaGly: 6.992 ± 0.101
1.332AlaHis: 1.332 ± 0.037
5.064AlaIle: 5.064 ± 0.081
5.164AlaLys: 5.164 ± 0.079
7.509AlaLeu: 7.509 ± 0.101
2.825AlaMet: 2.825 ± 0.052
2.652AlaAsn: 2.652 ± 0.053
2.296AlaPro: 2.296 ± 0.056
2.62AlaGln: 2.62 ± 0.051
3.175AlaArg: 3.175 ± 0.062
4.357AlaSer: 4.357 ± 0.074
3.328AlaThr: 3.328 ± 0.068
6.649AlaVal: 6.649 ± 0.091
0.712AlaTrp: 0.712 ± 0.025
2.753AlaTyr: 2.753 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
1.089CysAla: 1.089 ± 0.036
0.292CysCys: 0.292 ± 0.02
0.829CysAsp: 0.829 ± 0.029
0.872CysGlu: 0.872 ± 0.028
0.681CysPhe: 0.681 ± 0.024
1.543CysGly: 1.543 ± 0.04
0.314CysHis: 0.314 ± 0.019
1.073CysIle: 1.073 ± 0.034
0.791CysLys: 0.791 ± 0.036
1.297CysLeu: 1.297 ± 0.036
0.496CysMet: 0.496 ± 0.019
0.549CysAsn: 0.549 ± 0.023
0.714CysPro: 0.714 ± 0.029
0.458CysGln: 0.458 ± 0.023
0.763CysArg: 0.763 ± 0.028
0.973CysSer: 0.973 ± 0.033
0.755CysThr: 0.755 ± 0.031
1.069CysVal: 1.069 ± 0.037
0.123CysTrp: 0.123 ± 0.012
0.568CysTyr: 0.568 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.473AspAla: 4.473 ± 0.071
0.82AspCys: 0.82 ± 0.031
2.686AspAsp: 2.686 ± 0.062
4.275AspGlu: 4.275 ± 0.078
2.397AspPhe: 2.397 ± 0.044
4.489AspGly: 4.489 ± 0.089
1.081AspHis: 1.081 ± 0.045
3.935AspIle: 3.935 ± 0.064
2.891AspLys: 2.891 ± 0.057
4.762AspLeu: 4.762 ± 0.068
1.787AspMet: 1.787 ± 0.049
1.822AspAsn: 1.822 ± 0.046
2.1AspPro: 2.1 ± 0.054
1.763AspGln: 1.763 ± 0.048
2.519AspArg: 2.519 ± 0.05
2.947AspSer: 2.947 ± 0.061
2.942AspThr: 2.942 ± 0.065
3.758AspVal: 3.758 ± 0.064
0.614AspTrp: 0.614 ± 0.025
2.593AspTyr: 2.593 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
5.94GluAla: 5.94 ± 0.079
0.81GluCys: 0.81 ± 0.033
3.999GluAsp: 3.999 ± 0.063
6.787GluGlu: 6.787 ± 0.111
2.365GluPhe: 2.365 ± 0.052
4.114GluGly: 4.114 ± 0.072
1.671GluHis: 1.671 ± 0.045
5.095GluIle: 5.095 ± 0.084
6.186GluLys: 6.186 ± 0.092
6.861GluLeu: 6.861 ± 0.098
2.29GluMet: 2.29 ± 0.051
3.837GluAsn: 3.837 ± 0.069
1.98GluPro: 1.98 ± 0.054
3.296GluGln: 3.296 ± 0.066
3.625GluArg: 3.625 ± 0.064
3.46GluSer: 3.46 ± 0.071
4.317GluThr: 4.317 ± 0.08
4.382GluVal: 4.382 ± 0.071
0.637GluTrp: 0.637 ± 0.025
2.775GluTyr: 2.775 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.226PheAla: 3.226 ± 0.053
0.79PheCys: 0.79 ± 0.032
2.305PheAsp: 2.305 ± 0.048
2.541PheGlu: 2.541 ± 0.055
1.76PhePhe: 1.76 ± 0.053
3.203PheGly: 3.203 ± 0.058
0.901PheHis: 0.901 ± 0.029
2.392PheIle: 2.392 ± 0.061
1.704PheLys: 1.704 ± 0.047
4.042PheLeu: 4.042 ± 0.077
1.183PheMet: 1.183 ± 0.039
1.262PheAsn: 1.262 ± 0.041
1.471PhePro: 1.471 ± 0.038
1.372PheGln: 1.372 ± 0.042
1.644PheArg: 1.644 ± 0.039
2.802PheSer: 2.802 ± 0.051
2.275PheThr: 2.275 ± 0.054
2.791PheVal: 2.791 ± 0.053
0.446PheTrp: 0.446 ± 0.023
1.556PheTyr: 1.556 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
5.776GlyAla: 5.776 ± 0.099
1.412GlyCys: 1.412 ± 0.042
3.396GlyAsp: 3.396 ± 0.068
4.594GlyGlu: 4.594 ± 0.074
3.169GlyPhe: 3.169 ± 0.066
5.216GlyGly: 5.216 ± 0.115
1.373GlyHis: 1.373 ± 0.033
6.088GlyIle: 6.088 ± 0.074
5.32GlyLys: 5.32 ± 0.078
6.057GlyLeu: 6.057 ± 0.077
2.713GlyMet: 2.713 ± 0.062
3.236GlyAsn: 3.236 ± 0.07
1.534GlyPro: 1.534 ± 0.041
2.267GlyGln: 2.267 ± 0.051
3.183GlyArg: 3.183 ± 0.063
4.485GlySer: 4.485 ± 0.084
4.542GlyThr: 4.542 ± 0.071
5.197GlyVal: 5.197 ± 0.082
0.795GlyTrp: 0.795 ± 0.038
3.088GlyTyr: 3.088 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.386HisAla: 1.386 ± 0.042
0.325HisCys: 0.325 ± 0.022
0.994HisAsp: 0.994 ± 0.032
1.163HisGlu: 1.163 ± 0.042
0.861HisPhe: 0.861 ± 0.03
1.446HisGly: 1.446 ± 0.035
0.47HisHis: 0.47 ± 0.027
1.384HisIle: 1.384 ± 0.037
0.975HisLys: 0.975 ± 0.031
1.668HisLeu: 1.668 ± 0.046
0.651HisMet: 0.651 ± 0.029
0.749HisAsn: 0.749 ± 0.03
1.035HisPro: 1.035 ± 0.034
0.605HisGln: 0.605 ± 0.025
0.922HisArg: 0.922 ± 0.032
1.045HisSer: 1.045 ± 0.038
1.027HisThr: 1.027 ± 0.033
1.316HisVal: 1.316 ± 0.038
0.186HisTrp: 0.186 ± 0.014
0.799HisTyr: 0.799 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.691IleAla: 5.691 ± 0.093
1.304IleCys: 1.304 ± 0.036
3.567IleAsp: 3.567 ± 0.061
4.075IleGlu: 4.075 ± 0.074
2.547IlePhe: 2.547 ± 0.054
5.124IleGly: 5.124 ± 0.088
1.373IleHis: 1.373 ± 0.033
4.266IleIle: 4.266 ± 0.076
3.28IleLys: 3.28 ± 0.072
6.9IleLeu: 6.9 ± 0.1
1.972IleMet: 1.972 ± 0.048
2.434IleAsn: 2.434 ± 0.049
3.369IlePro: 3.369 ± 0.053
2.392IleGln: 2.392 ± 0.051
3.965IleArg: 3.965 ± 0.077
4.438IleSer: 4.438 ± 0.069
3.911IleThr: 3.911 ± 0.067
4.541IleVal: 4.541 ± 0.077
0.637IleTrp: 0.637 ± 0.024
2.363IleTyr: 2.363 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
5.207LysAla: 5.207 ± 0.082
0.586LysCys: 0.586 ± 0.025
3.468LysAsp: 3.468 ± 0.057
6.055LysGlu: 6.055 ± 0.09
1.678LysPhe: 1.678 ± 0.048
3.696LysGly: 3.696 ± 0.065
1.097LysHis: 1.097 ± 0.033
4.068LysIle: 4.068 ± 0.069
5.407LysLys: 5.407 ± 0.093
5.403LysLeu: 5.403 ± 0.079
2.016LysMet: 2.016 ± 0.046
3.2LysAsn: 3.2 ± 0.061
2.123LysPro: 2.123 ± 0.05
2.588LysGln: 2.588 ± 0.058
3.024LysArg: 3.024 ± 0.062
3.075LysSer: 3.075 ± 0.062
3.771LysThr: 3.771 ± 0.073
3.843LysVal: 3.843 ± 0.068
0.564LysTrp: 0.564 ± 0.025
2.344LysTyr: 2.344 ± 0.059
0.0LysXaa: 0.0 ± 0.0
Leu
7.418LeuAla: 7.418 ± 0.101
1.549LeuCys: 1.549 ± 0.035
5.112LeuAsp: 5.112 ± 0.077
6.559LeuGlu: 6.559 ± 0.092
3.837LeuPhe: 3.837 ± 0.079
6.33LeuGly: 6.33 ± 0.089
1.715LeuHis: 1.715 ± 0.048
5.832LeuIle: 5.832 ± 0.097
6.179LeuLys: 6.179 ± 0.086
8.711LeuLeu: 8.711 ± 0.13
2.803LeuMet: 2.803 ± 0.057
3.742LeuAsn: 3.742 ± 0.065
3.755LeuPro: 3.755 ± 0.069
2.989LeuGln: 2.989 ± 0.062
3.918LeuArg: 3.918 ± 0.067
6.171LeuSer: 6.171 ± 0.091
5.15LeuThr: 5.15 ± 0.075
5.872LeuVal: 5.872 ± 0.089
0.748LeuTrp: 0.748 ± 0.031
3.186LeuTyr: 3.186 ± 0.062
0.0LeuXaa: 0.0 ± 0.0
Met
2.743MetAla: 2.743 ± 0.051
0.356MetCys: 0.356 ± 0.022
1.938MetAsp: 1.938 ± 0.045
2.655MetGlu: 2.655 ± 0.055
1.134MetPhe: 1.134 ± 0.035
2.174MetGly: 2.174 ± 0.053
0.504MetHis: 0.504 ± 0.023
2.187MetIle: 2.187 ± 0.051
2.499MetLys: 2.499 ± 0.05
2.867MetLeu: 2.867 ± 0.061
1.036MetMet: 1.036 ± 0.033
1.526MetAsn: 1.526 ± 0.038
1.264MetPro: 1.264 ± 0.035
1.14MetGln: 1.14 ± 0.034
1.427MetArg: 1.427 ± 0.043
1.815MetSer: 1.815 ± 0.045
1.864MetThr: 1.864 ± 0.046
2.105MetVal: 2.105 ± 0.049
0.196MetTrp: 0.196 ± 0.013
0.803MetTyr: 0.803 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.187AsnAla: 3.187 ± 0.065
0.587AsnCys: 0.587 ± 0.026
1.878AsnAsp: 1.878 ± 0.042
2.496AsnGlu: 2.496 ± 0.047
1.505AsnPhe: 1.505 ± 0.042
3.344AsnGly: 3.344 ± 0.075
0.848AsnHis: 0.848 ± 0.031
2.787AsnIle: 2.787 ± 0.055
2.011AsnLys: 2.011 ± 0.047
3.732AsnLeu: 3.732 ± 0.074
1.338AsnMet: 1.338 ± 0.036
1.518AsnAsn: 1.518 ± 0.046
2.111AsnPro: 2.111 ± 0.047
1.585AsnGln: 1.585 ± 0.036
2.199AsnArg: 2.199 ± 0.055
2.12AsnSer: 2.12 ± 0.049
2.146AsnThr: 2.146 ± 0.048
2.728AsnVal: 2.728 ± 0.049
0.39AsnTrp: 0.39 ± 0.022
1.585AsnTyr: 1.585 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
2.867ProAla: 2.867 ± 0.053
0.489ProCys: 0.489 ± 0.022
2.586ProAsp: 2.586 ± 0.05
3.734ProGlu: 3.734 ± 0.062
1.599ProPhe: 1.599 ± 0.045
2.757ProGly: 2.757 ± 0.055
0.639ProHis: 0.639 ± 0.028
2.056ProIle: 2.056 ± 0.047
1.937ProLys: 1.937 ± 0.048
2.992ProLeu: 2.992 ± 0.059
1.036ProMet: 1.036 ± 0.03
1.24ProAsn: 1.24 ± 0.034
0.782ProPro: 0.782 ± 0.027
1.222ProGln: 1.222 ± 0.035
1.11ProArg: 1.11 ± 0.041
1.87ProSer: 1.87 ± 0.051
1.643ProThr: 1.643 ± 0.041
3.325ProVal: 3.325 ± 0.063
0.345ProTrp: 0.345 ± 0.019
1.526ProTyr: 1.526 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.821GlnAla: 2.821 ± 0.061
0.403GlnCys: 0.403 ± 0.021
1.718GlnAsp: 1.718 ± 0.043
2.926GlnGlu: 2.926 ± 0.059
1.268GlnPhe: 1.268 ± 0.038
2.144GlnGly: 2.144 ± 0.051
0.521GlnHis: 0.521 ± 0.026
2.84GlnIle: 2.84 ± 0.058
2.758GlnLys: 2.758 ± 0.057
3.106GlnLeu: 3.106 ± 0.054
1.288GlnMet: 1.288 ± 0.038
1.71GlnAsn: 1.71 ± 0.052
1.063GlnPro: 1.063 ± 0.036
1.353GlnGln: 1.353 ± 0.045
1.382GlnArg: 1.382 ± 0.038
1.851GlnSer: 1.851 ± 0.049
2.018GlnThr: 2.018 ± 0.048
2.351GlnVal: 2.351 ± 0.056
0.315GlnTrp: 0.315 ± 0.018
1.472GlnTyr: 1.472 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
3.058ArgAla: 3.058 ± 0.068
0.674ArgCys: 0.674 ± 0.025
2.328ArgAsp: 2.328 ± 0.048
3.848ArgGlu: 3.848 ± 0.069
1.905ArgPhe: 1.905 ± 0.039
2.583ArgGly: 2.583 ± 0.053
0.889ArgHis: 0.889 ± 0.035
3.484ArgIle: 3.484 ± 0.063
3.453ArgLys: 3.453 ± 0.068
4.124ArgLeu: 4.124 ± 0.061
1.75ArgMet: 1.75 ± 0.036
2.058ArgAsn: 2.058 ± 0.046
1.487ArgPro: 1.487 ± 0.041
1.971ArgGln: 1.971 ± 0.053
2.322ArgArg: 2.322 ± 0.049
2.41ArgSer: 2.41 ± 0.049
2.404ArgThr: 2.404 ± 0.054
2.912ArgVal: 2.912 ± 0.054
0.39ArgTrp: 0.39 ± 0.022
2.032ArgTyr: 2.032 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
4.519SerAla: 4.519 ± 0.086
0.851SerCys: 0.851 ± 0.032
3.113SerAsp: 3.113 ± 0.064
3.87SerGlu: 3.87 ± 0.069
2.561SerPhe: 2.561 ± 0.055
5.344SerGly: 5.344 ± 0.091
1.124SerHis: 1.124 ± 0.034
3.847SerIle: 3.847 ± 0.076
2.98SerLys: 2.98 ± 0.053
5.027SerLeu: 5.027 ± 0.091
2.012SerMet: 2.012 ± 0.044
2.008SerAsn: 2.008 ± 0.052
1.842SerPro: 1.842 ± 0.046
1.935SerGln: 1.935 ± 0.049
2.929SerArg: 2.929 ± 0.062
3.508SerSer: 3.508 ± 0.089
2.748SerThr: 2.748 ± 0.052
4.222SerVal: 4.222 ± 0.071
0.615SerTrp: 0.615 ± 0.024
2.274SerTyr: 2.274 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
5.102ThrAla: 5.102 ± 0.087
0.704ThrCys: 0.704 ± 0.029
3.152ThrAsp: 3.152 ± 0.064
3.867ThrGlu: 3.867 ± 0.073
2.105ThrPhe: 2.105 ± 0.051
4.989ThrGly: 4.989 ± 0.082
0.894ThrHis: 0.894 ± 0.03
3.791ThrIle: 3.791 ± 0.067
2.799ThrLys: 2.799 ± 0.054
4.712ThrLeu: 4.712 ± 0.074
1.577ThrMet: 1.577 ± 0.043
1.891ThrAsn: 1.891 ± 0.042
2.345ThrPro: 2.345 ± 0.05
1.536ThrGln: 1.536 ± 0.044
2.244ThrArg: 2.244 ± 0.046
2.883ThrSer: 2.883 ± 0.054
2.852ThrThr: 2.852 ± 0.066
4.463ThrVal: 4.463 ± 0.088
0.572ThrTrp: 0.572 ± 0.028
1.951ThrTyr: 1.951 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
5.207ValAla: 5.207 ± 0.084
1.252ValCys: 1.252 ± 0.031
3.778ValAsp: 3.778 ± 0.065
4.511ValGlu: 4.511 ± 0.075
2.947ValPhe: 2.947 ± 0.06
4.484ValGly: 4.484 ± 0.075
1.173ValHis: 1.173 ± 0.042
4.99ValIle: 4.99 ± 0.087
4.171ValLys: 4.171 ± 0.063
7.165ValLeu: 7.165 ± 0.105
2.129ValMet: 2.129 ± 0.049
2.626ValAsn: 2.626 ± 0.059
2.733ValPro: 2.733 ± 0.044
2.201ValGln: 2.201 ± 0.057
3.231ValArg: 3.231 ± 0.054
4.599ValSer: 4.599 ± 0.076
4.251ValThr: 4.251 ± 0.085
4.89ValVal: 4.89 ± 0.09
0.604ValTrp: 0.604 ± 0.027
2.56ValTyr: 2.56 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.533TrpAla: 0.533 ± 0.022
0.138TrpCys: 0.138 ± 0.014
0.525TrpAsp: 0.525 ± 0.025
0.651TrpGlu: 0.651 ± 0.029
0.391TrpPhe: 0.391 ± 0.021
0.582TrpGly: 0.582 ± 0.023
0.18TrpHis: 0.18 ± 0.013
0.727TrpIle: 0.727 ± 0.028
0.798TrpLys: 0.798 ± 0.028
0.922TrpLeu: 0.922 ± 0.032
0.312TrpMet: 0.312 ± 0.018
0.54TrpAsn: 0.54 ± 0.027
0.211TrpPro: 0.211 ± 0.014
0.405TrpGln: 0.405 ± 0.02
0.398TrpArg: 0.398 ± 0.021
0.522TrpSer: 0.522 ± 0.027
0.432TrpThr: 0.432 ± 0.021
0.552TrpVal: 0.552 ± 0.025
0.108TrpTrp: 0.108 ± 0.011
0.422TrpTyr: 0.422 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.87TyrAla: 2.87 ± 0.047
0.619TyrCys: 0.619 ± 0.022
2.351TyrAsp: 2.351 ± 0.053
2.755TyrGlu: 2.755 ± 0.06
1.675TyrPhe: 1.675 ± 0.039
2.885TyrGly: 2.885 ± 0.054
0.91TyrHis: 0.91 ± 0.03
2.244TyrIle: 2.244 ± 0.051
1.914TyrLys: 1.914 ± 0.049
3.717TyrLeu: 3.717 ± 0.071
1.016TyrMet: 1.016 ± 0.034
1.498TyrAsn: 1.498 ± 0.044
1.54TyrPro: 1.54 ± 0.039
1.612TyrGln: 1.612 ± 0.048
2.101TyrArg: 2.101 ± 0.043
1.998TyrSer: 1.998 ± 0.047
2.053TyrThr: 2.053 ± 0.056
2.536TyrVal: 2.536 ± 0.05
0.352TyrTrp: 0.352 ± 0.019
1.778TyrTyr: 1.778 ± 0.057
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3013 proteins (978987 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski