Amino acid dipepetide frequency for Mycobacterium phage Dulcie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.131AlaAla: 13.131 ± 1.419
0.657AlaCys: 0.657 ± 0.189
7.342AlaAsp: 7.342 ± 0.691
6.088AlaGlu: 6.088 ± 0.668
3.701AlaPhe: 3.701 ± 0.463
8.177AlaGly: 8.177 ± 0.81
1.313AlaHis: 1.313 ± 0.327
4.656AlaIle: 4.656 ± 0.597
3.641AlaLys: 3.641 ± 0.465
8.655AlaLeu: 8.655 ± 0.784
2.686AlaMet: 2.686 ± 0.406
2.746AlaAsn: 2.746 ± 0.364
4.894AlaPro: 4.894 ± 0.678
3.342AlaGln: 3.342 ± 0.547
5.909AlaArg: 5.909 ± 0.569
5.073AlaSer: 5.073 ± 0.57
6.028AlaThr: 6.028 ± 0.56
8.476AlaVal: 8.476 ± 0.812
1.791AlaTrp: 1.791 ± 0.296
2.686AlaTyr: 2.686 ± 0.403
0.0AlaXaa: 0.0 ± 0.0
Cys
0.597CysAla: 0.597 ± 0.19
0.0CysCys: 0.0 ± 0.0
0.358CysAsp: 0.358 ± 0.16
0.597CysGlu: 0.597 ± 0.152
0.179CysPhe: 0.179 ± 0.093
0.657CysGly: 0.657 ± 0.231
0.119CysHis: 0.119 ± 0.087
0.239CysIle: 0.239 ± 0.108
0.179CysLys: 0.179 ± 0.116
0.537CysLeu: 0.537 ± 0.175
0.06CysMet: 0.06 ± 0.06
0.179CysAsn: 0.179 ± 0.098
0.298CysPro: 0.298 ± 0.132
0.179CysGln: 0.179 ± 0.102
0.537CysArg: 0.537 ± 0.169
0.537CysSer: 0.537 ± 0.176
0.477CysThr: 0.477 ± 0.154
0.239CysVal: 0.239 ± 0.125
0.298CysTrp: 0.298 ± 0.131
0.119CysTyr: 0.119 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
6.625AspAla: 6.625 ± 0.639
0.716AspCys: 0.716 ± 0.197
4.775AspAsp: 4.775 ± 0.46
3.641AspGlu: 3.641 ± 0.41
2.447AspPhe: 2.447 ± 0.361
6.566AspGly: 6.566 ± 0.571
1.194AspHis: 1.194 ± 0.277
2.805AspIle: 2.805 ± 0.4
2.387AspLys: 2.387 ± 0.391
7.222AspLeu: 7.222 ± 0.667
1.074AspMet: 1.074 ± 0.218
2.029AspAsn: 2.029 ± 0.336
4.656AspPro: 4.656 ± 0.517
2.029AspGln: 2.029 ± 0.43
4.417AspArg: 4.417 ± 0.59
3.104AspSer: 3.104 ± 0.462
3.522AspThr: 3.522 ± 0.453
5.014AspVal: 5.014 ± 0.523
1.791AspTrp: 1.791 ± 0.345
2.149AspTyr: 2.149 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
5.969GluAla: 5.969 ± 0.581
0.418GluCys: 0.418 ± 0.172
4.954GluAsp: 4.954 ± 0.557
4.536GluGlu: 4.536 ± 0.527
1.791GluPhe: 1.791 ± 0.367
3.76GluGly: 3.76 ± 0.436
1.253GluHis: 1.253 ± 0.293
3.283GluIle: 3.283 ± 0.49
2.865GluLys: 2.865 ± 0.444
6.983GluLeu: 6.983 ± 0.6
1.612GluMet: 1.612 ± 0.258
1.731GluAsn: 1.731 ± 0.363
2.208GluPro: 2.208 ± 0.403
2.447GluGln: 2.447 ± 0.395
3.88GluArg: 3.88 ± 0.51
3.522GluSer: 3.522 ± 0.494
3.939GluThr: 3.939 ± 0.448
5.252GluVal: 5.252 ± 0.547
1.492GluTrp: 1.492 ± 0.353
2.567GluTyr: 2.567 ± 0.402
0.0GluXaa: 0.0 ± 0.0
Phe
2.447PheAla: 2.447 ± 0.271
0.358PheCys: 0.358 ± 0.17
2.507PheAsp: 2.507 ± 0.336
2.029PheGlu: 2.029 ± 0.363
0.597PhePhe: 0.597 ± 0.161
3.462PheGly: 3.462 ± 0.472
0.836PheHis: 0.836 ± 0.22
1.253PheIle: 1.253 ± 0.242
1.313PheLys: 1.313 ± 0.276
2.447PheLeu: 2.447 ± 0.39
0.597PheMet: 0.597 ± 0.192
0.955PheAsn: 0.955 ± 0.191
1.492PhePro: 1.492 ± 0.317
0.776PheGln: 0.776 ± 0.187
1.97PheArg: 1.97 ± 0.315
2.208PheSer: 2.208 ± 0.464
2.149PheThr: 2.149 ± 0.339
2.208PheVal: 2.208 ± 0.42
0.597PheTrp: 0.597 ± 0.188
0.955PheTyr: 0.955 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
7.401GlyAla: 7.401 ± 0.987
0.537GlyCys: 0.537 ± 0.174
6.088GlyAsp: 6.088 ± 0.593
4.178GlyGlu: 4.178 ± 0.522
2.746GlyPhe: 2.746 ± 0.467
9.252GlyGly: 9.252 ± 2.009
2.029GlyHis: 2.029 ± 0.332
4.178GlyIle: 4.178 ± 0.594
3.701GlyLys: 3.701 ± 0.414
7.938GlyLeu: 7.938 ± 0.912
1.552GlyMet: 1.552 ± 0.297
3.163GlyAsn: 3.163 ± 0.439
3.88GlyPro: 3.88 ± 0.633
2.387GlyGln: 2.387 ± 0.325
5.014GlyArg: 5.014 ± 0.513
6.625GlySer: 6.625 ± 0.916
5.611GlyThr: 5.611 ± 0.719
5.611GlyVal: 5.611 ± 0.539
2.387GlyTrp: 2.387 ± 0.402
3.044GlyTyr: 3.044 ± 0.371
0.0GlyXaa: 0.0 ± 0.0
His
1.791HisAla: 1.791 ± 0.377
0.119HisCys: 0.119 ± 0.121
1.313HisAsp: 1.313 ± 0.253
1.671HisGlu: 1.671 ± 0.324
0.716HisPhe: 0.716 ± 0.21
1.731HisGly: 1.731 ± 0.358
0.657HisHis: 0.657 ± 0.169
0.836HisIle: 0.836 ± 0.2
0.955HisLys: 0.955 ± 0.271
1.194HisLeu: 1.194 ± 0.268
0.119HisMet: 0.119 ± 0.083
0.239HisAsn: 0.239 ± 0.113
1.492HisPro: 1.492 ± 0.278
0.716HisGln: 0.716 ± 0.204
1.552HisArg: 1.552 ± 0.304
0.418HisSer: 0.418 ± 0.155
0.895HisThr: 0.895 ± 0.261
1.671HisVal: 1.671 ± 0.309
0.477HisTrp: 0.477 ± 0.165
0.657HisTyr: 0.657 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
6.387IleAla: 6.387 ± 0.784
0.298IleCys: 0.298 ± 0.131
3.044IleAsp: 3.044 ± 0.354
3.701IleGlu: 3.701 ± 0.399
1.074IlePhe: 1.074 ± 0.264
3.701IleGly: 3.701 ± 0.418
0.776IleHis: 0.776 ± 0.208
1.552IleIle: 1.552 ± 0.313
1.612IleLys: 1.612 ± 0.348
3.88IleLeu: 3.88 ± 0.413
0.716IleMet: 0.716 ± 0.212
1.97IleAsn: 1.97 ± 0.292
2.925IlePro: 2.925 ± 0.361
1.492IleGln: 1.492 ± 0.342
3.581IleArg: 3.581 ± 0.443
3.223IleSer: 3.223 ± 0.456
3.104IleThr: 3.104 ± 0.437
2.865IleVal: 2.865 ± 0.492
0.836IleTrp: 0.836 ± 0.197
1.492IleTyr: 1.492 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
3.581LysAla: 3.581 ± 0.518
0.298LysCys: 0.298 ± 0.142
2.507LysAsp: 2.507 ± 0.344
1.97LysGlu: 1.97 ± 0.382
1.492LysPhe: 1.492 ± 0.305
2.328LysGly: 2.328 ± 0.313
1.313LysHis: 1.313 ± 0.315
2.507LysIle: 2.507 ± 0.349
2.447LysLys: 2.447 ± 0.468
3.522LysLeu: 3.522 ± 0.467
0.955LysMet: 0.955 ± 0.197
1.373LysAsn: 1.373 ± 0.279
2.746LysPro: 2.746 ± 0.442
1.612LysGln: 1.612 ± 0.327
2.984LysArg: 2.984 ± 0.496
2.507LysSer: 2.507 ± 0.38
2.387LysThr: 2.387 ± 0.37
3.223LysVal: 3.223 ± 0.427
0.716LysTrp: 0.716 ± 0.189
0.836LysTyr: 0.836 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
9.908LeuAla: 9.908 ± 0.867
0.179LeuCys: 0.179 ± 0.098
6.387LeuAsp: 6.387 ± 0.518
5.312LeuGlu: 5.312 ± 0.579
1.91LeuPhe: 1.91 ± 0.381
7.58LeuGly: 7.58 ± 0.82
1.612LeuHis: 1.612 ± 0.31
5.014LeuIle: 5.014 ± 0.535
3.999LeuLys: 3.999 ± 0.522
5.551LeuLeu: 5.551 ± 0.495
1.552LeuMet: 1.552 ± 0.261
2.805LeuAsn: 2.805 ± 0.362
5.312LeuPro: 5.312 ± 0.523
2.626LeuGln: 2.626 ± 0.419
6.148LeuArg: 6.148 ± 0.588
5.909LeuSer: 5.909 ± 0.612
5.909LeuThr: 5.909 ± 0.49
4.477LeuVal: 4.477 ± 0.596
1.194LeuTrp: 1.194 ± 0.31
2.208LeuTyr: 2.208 ± 0.346
0.0LeuXaa: 0.0 ± 0.0
Met
2.507MetAla: 2.507 ± 0.281
0.0MetCys: 0.0 ± 0.0
1.612MetAsp: 1.612 ± 0.312
1.373MetGlu: 1.373 ± 0.313
0.477MetPhe: 0.477 ± 0.144
1.373MetGly: 1.373 ± 0.27
0.298MetHis: 0.298 ± 0.13
0.716MetIle: 0.716 ± 0.227
0.716MetLys: 0.716 ± 0.2
1.015MetLeu: 1.015 ± 0.239
0.179MetMet: 0.179 ± 0.109
1.015MetAsn: 1.015 ± 0.243
1.134MetPro: 1.134 ± 0.278
0.477MetGln: 0.477 ± 0.135
1.313MetArg: 1.313 ± 0.242
2.029MetSer: 2.029 ± 0.344
1.85MetThr: 1.85 ± 0.293
1.194MetVal: 1.194 ± 0.275
0.298MetTrp: 0.298 ± 0.122
0.358MetTyr: 0.358 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
2.865AsnAla: 2.865 ± 0.492
0.179AsnCys: 0.179 ± 0.108
2.089AsnAsp: 2.089 ± 0.356
1.731AsnGlu: 1.731 ± 0.328
0.895AsnPhe: 0.895 ± 0.237
3.402AsnGly: 3.402 ± 0.511
0.597AsnHis: 0.597 ± 0.159
1.791AsnIle: 1.791 ± 0.285
0.298AsnLys: 0.298 ± 0.144
2.149AsnLeu: 2.149 ± 0.336
0.716AsnMet: 0.716 ± 0.219
1.253AsnAsn: 1.253 ± 0.371
2.626AsnPro: 2.626 ± 0.372
1.134AsnGln: 1.134 ± 0.246
1.552AsnArg: 1.552 ± 0.345
2.447AsnSer: 2.447 ± 0.457
1.731AsnThr: 1.731 ± 0.319
2.268AsnVal: 2.268 ± 0.453
0.657AsnTrp: 0.657 ± 0.179
1.313AsnTyr: 1.313 ± 0.317
0.0AsnXaa: 0.0 ± 0.0
Pro
5.551ProAla: 5.551 ± 0.596
0.179ProCys: 0.179 ± 0.103
3.939ProAsp: 3.939 ± 0.465
4.059ProGlu: 4.059 ± 0.458
2.268ProPhe: 2.268 ± 0.366
4.835ProGly: 4.835 ± 0.611
0.836ProHis: 0.836 ± 0.232
2.208ProIle: 2.208 ± 0.408
2.328ProLys: 2.328 ± 0.342
4.297ProLeu: 4.297 ± 0.489
0.776ProMet: 0.776 ± 0.211
1.194ProAsn: 1.194 ± 0.299
2.746ProPro: 2.746 ± 0.422
1.612ProGln: 1.612 ± 0.417
3.044ProArg: 3.044 ± 0.498
3.641ProSer: 3.641 ± 0.474
3.939ProThr: 3.939 ± 0.576
3.641ProVal: 3.641 ± 0.43
0.716ProTrp: 0.716 ± 0.27
1.671ProTyr: 1.671 ± 0.363
0.0ProXaa: 0.0 ± 0.0
Gln
3.104GlnAla: 3.104 ± 0.472
0.179GlnCys: 0.179 ± 0.102
1.194GlnAsp: 1.194 ± 0.316
2.149GlnGlu: 2.149 ± 0.316
1.253GlnPhe: 1.253 ± 0.261
2.686GlnGly: 2.686 ± 0.311
0.537GlnHis: 0.537 ± 0.143
2.805GlnIle: 2.805 ± 0.551
1.194GlnLys: 1.194 ± 0.236
3.641GlnLeu: 3.641 ± 0.454
0.776GlnMet: 0.776 ± 0.267
0.477GlnAsn: 0.477 ± 0.163
1.97GlnPro: 1.97 ± 0.361
1.731GlnGln: 1.731 ± 0.365
1.731GlnArg: 1.731 ± 0.328
1.731GlnSer: 1.731 ± 0.3
1.85GlnThr: 1.85 ± 0.249
2.387GlnVal: 2.387 ± 0.366
0.597GlnTrp: 0.597 ± 0.141
0.537GlnTyr: 0.537 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
6.028ArgAla: 6.028 ± 0.695
0.836ArgCys: 0.836 ± 0.234
3.641ArgAsp: 3.641 ± 0.477
4.715ArgGlu: 4.715 ± 0.591
1.97ArgPhe: 1.97 ± 0.339
4.954ArgGly: 4.954 ± 0.619
0.955ArgHis: 0.955 ± 0.231
3.462ArgIle: 3.462 ± 0.517
3.342ArgLys: 3.342 ± 0.505
6.327ArgLeu: 6.327 ± 0.726
1.612ArgMet: 1.612 ± 0.343
2.387ArgAsn: 2.387 ± 0.482
2.208ArgPro: 2.208 ± 0.356
1.91ArgGln: 1.91 ± 0.328
5.611ArgArg: 5.611 ± 0.709
3.82ArgSer: 3.82 ± 0.585
3.641ArgThr: 3.641 ± 0.536
5.193ArgVal: 5.193 ± 0.577
1.074ArgTrp: 1.074 ± 0.272
1.731ArgTyr: 1.731 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
6.207SerAla: 6.207 ± 0.726
0.477SerCys: 0.477 ± 0.194
3.462SerAsp: 3.462 ± 0.506
3.939SerGlu: 3.939 ± 0.523
1.612SerPhe: 1.612 ± 0.315
6.864SerGly: 6.864 ± 0.934
1.492SerHis: 1.492 ± 0.274
2.387SerIle: 2.387 ± 0.387
2.746SerLys: 2.746 ± 0.359
5.014SerLeu: 5.014 ± 0.594
1.612SerMet: 1.612 ± 0.348
2.328SerAsn: 2.328 ± 0.436
2.984SerPro: 2.984 ± 0.376
1.552SerGln: 1.552 ± 0.257
3.76SerArg: 3.76 ± 0.414
2.925SerSer: 2.925 ± 0.569
3.342SerThr: 3.342 ± 0.465
4.417SerVal: 4.417 ± 0.474
1.134SerTrp: 1.134 ± 0.308
1.194SerTyr: 1.194 ± 0.273
0.0SerXaa: 0.0 ± 0.0
Thr
6.148ThrAla: 6.148 ± 0.827
0.239ThrCys: 0.239 ± 0.115
3.999ThrAsp: 3.999 ± 0.458
4.118ThrGlu: 4.118 ± 0.457
2.149ThrPhe: 2.149 ± 0.357
7.043ThrGly: 7.043 ± 0.653
0.895ThrHis: 0.895 ± 0.273
2.686ThrIle: 2.686 ± 0.522
2.686ThrLys: 2.686 ± 0.309
5.969ThrLeu: 5.969 ± 0.602
0.895ThrMet: 0.895 ± 0.173
1.612ThrAsn: 1.612 ± 0.355
3.939ThrPro: 3.939 ± 0.54
1.97ThrGln: 1.97 ± 0.316
3.581ThrArg: 3.581 ± 0.501
3.462ThrSer: 3.462 ± 0.504
4.417ThrThr: 4.417 ± 0.597
5.432ThrVal: 5.432 ± 0.635
0.955ThrTrp: 0.955 ± 0.24
1.91ThrTyr: 1.91 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
6.864ValAla: 6.864 ± 0.696
0.298ValCys: 0.298 ± 0.142
5.849ValAsp: 5.849 ± 0.579
5.312ValGlu: 5.312 ± 0.578
2.447ValPhe: 2.447 ± 0.351
4.835ValGly: 4.835 ± 0.691
1.373ValHis: 1.373 ± 0.251
3.342ValIle: 3.342 ± 0.452
3.283ValLys: 3.283 ± 0.396
5.193ValLeu: 5.193 ± 0.536
1.253ValMet: 1.253 ± 0.329
2.387ValAsn: 2.387 ± 0.37
3.88ValPro: 3.88 ± 0.452
2.387ValGln: 2.387 ± 0.392
5.312ValArg: 5.312 ± 0.716
4.536ValSer: 4.536 ± 0.432
5.73ValThr: 5.73 ± 0.602
4.715ValVal: 4.715 ± 0.576
1.313ValTrp: 1.313 ± 0.246
1.97ValTyr: 1.97 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
1.373TrpAla: 1.373 ± 0.301
0.179TrpCys: 0.179 ± 0.091
1.671TrpAsp: 1.671 ± 0.333
1.074TrpGlu: 1.074 ± 0.187
0.776TrpPhe: 0.776 ± 0.242
1.492TrpGly: 1.492 ± 0.264
0.537TrpHis: 0.537 ± 0.172
0.955TrpIle: 0.955 ± 0.204
0.358TrpLys: 0.358 ± 0.216
1.671TrpLeu: 1.671 ± 0.314
0.477TrpMet: 0.477 ± 0.174
0.477TrpAsn: 0.477 ± 0.195
0.836TrpPro: 0.836 ± 0.227
1.074TrpGln: 1.074 ± 0.234
1.015TrpArg: 1.015 ± 0.338
0.776TrpSer: 0.776 ± 0.2
1.791TrpThr: 1.791 ± 0.365
2.268TrpVal: 2.268 ± 0.33
0.776TrpTrp: 0.776 ± 0.266
0.298TrpTyr: 0.298 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.507TyrAla: 2.507 ± 0.387
0.239TyrCys: 0.239 ± 0.149
1.432TyrAsp: 1.432 ± 0.305
2.268TyrGlu: 2.268 ± 0.312
0.657TyrPhe: 0.657 ± 0.176
2.626TyrGly: 2.626 ± 0.351
0.597TyrHis: 0.597 ± 0.185
1.552TyrIle: 1.552 ± 0.338
1.253TyrLys: 1.253 ± 0.278
2.387TyrLeu: 2.387 ± 0.339
0.657TyrMet: 0.657 ± 0.184
1.373TyrAsn: 1.373 ± 0.294
1.313TyrPro: 1.313 ± 0.257
1.074TyrGln: 1.074 ± 0.287
2.507TyrArg: 2.507 ± 0.341
0.955TyrSer: 0.955 ± 0.224
1.791TyrThr: 1.791 ± 0.333
1.731TyrVal: 1.731 ± 0.335
0.776TyrTrp: 0.776 ± 0.242
0.716TyrTyr: 0.716 ± 0.282
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (16755 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski