Candidatus Paraburkholderia kirkii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0LZI0|A0A0L0LZI0_9BURK Uncharacterized protein OS=Candidatus Paraburkholderia kirkii OX=198822 GN=BPUN_3192 PE=4 SV=1
MM1 pKa = 7.72AVTQQVTNPVANFADD16 pKa = 4.23DD17 pKa = 3.88NNGVLIEE24 pKa = 4.84LPDD27 pKa = 3.84VPDD30 pKa = 5.42DD31 pKa = 4.04GASMVTGTMTFGIGSQSNNLLGSATVLKK59 pKa = 10.28SDD61 pKa = 3.53SVTGYY66 pKa = 8.65VTTDD70 pKa = 3.62FDD72 pKa = 4.12GSQYY76 pKa = 10.91GSSFIDD82 pKa = 3.29SGSNGLFFPSTTLFQCGAWYY102 pKa = 10.31CPATTQAFSATIRR115 pKa = 11.84SSSGAQSAVGFSVAQSTEE133 pKa = 3.76LLGTGNYY140 pKa = 10.57AFDD143 pKa = 4.23NLAGAASGYY152 pKa = 10.09FDD154 pKa = 3.1WGLPFFYY161 pKa = 10.32GRR163 pKa = 11.84RR164 pKa = 11.84VFTAIEE170 pKa = 4.23GRR172 pKa = 11.84ATPAGAGPYY181 pKa = 9.69YY182 pKa = 10.98AFF184 pKa = 5.07

Molecular weight: 19.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0M1T3|A0A0L0M1T3_9BURK Histidine kinase OS=Candidatus Paraburkholderia kirkii OX=198822 GN=BPUN_1635 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.74QPSMTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.1RR14 pKa = 11.84THH16 pKa = 5.75GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2202

0

2202

663521

14

1567

301.3

32.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.024 ± 0.067

0.995 ± 0.017

5.753 ± 0.039

5.708 ± 0.052

3.729 ± 0.032

7.879 ± 0.043

2.389 ± 0.023

4.885 ± 0.034

3.76 ± 0.043

9.809 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.022

2.887 ± 0.029

4.921 ± 0.037

3.444 ± 0.037

7.349 ± 0.052

5.525 ± 0.04

5.151 ± 0.034

7.591 ± 0.041

1.271 ± 0.021

2.437 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski