Insolitispirillum peregrinum

Taxonomy:

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7LIB3|A0A1N7LIB3_9PROT Transcriptional regulator BadM/Rrf2 family OS=Insolitispirillum peregrinum OX=80876 GN=SAMN05421779_103347 PE=4 SV=1
MM1 pKa = 7.38TMRR4 pKa = 11.84PPMYY8 pKa = 10.18VYY10 pKa = 10.2DD11 pKa = 4.52APPPPSSEE19 pKa = 4.05DD20 pKa = 3.51AIGRR24 pKa = 11.84AGEE27 pKa = 4.07PLWPGAEE34 pKa = 4.02IATHH38 pKa = 5.59EE39 pKa = 4.36ALVEE43 pKa = 3.97ALQTVYY49 pKa = 10.97DD50 pKa = 3.9PEE52 pKa = 5.22IPVNIYY58 pKa = 10.81DD59 pKa = 4.71LGLIYY64 pKa = 10.75DD65 pKa = 4.5LTISDD70 pKa = 4.28NGTVAALMTLTAPACPVAGEE90 pKa = 4.79LPQQVANTLSLVKK103 pKa = 9.94GTGEE107 pKa = 4.09VTVTLTWDD115 pKa = 3.89PPWTPEE121 pKa = 4.21RR122 pKa = 11.84MTEE125 pKa = 4.09VARR128 pKa = 11.84VALDD132 pKa = 3.24MFF134 pKa = 5.24

Molecular weight: 14.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7MGN6|A0A1N7MGN6_9PROT Single-stranded DNA-binding protein OS=Insolitispirillum peregrinum OX=80876 GN=SAMN05421779_104110 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.27VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QGRR39 pKa = 11.84KK40 pKa = 9.15RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3976

0

3976

1355373

25

3980

340.9

36.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.091 ± 0.047

0.988 ± 0.013

5.787 ± 0.038

5.232 ± 0.038

3.284 ± 0.023

8.17 ± 0.037

2.234 ± 0.018

4.708 ± 0.028

2.959 ± 0.036

10.956 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.021

2.456 ± 0.022

5.375 ± 0.034

3.923 ± 0.033

6.695 ± 0.041

5.93 ± 0.042

5.57 ± 0.064

7.597 ± 0.032

1.33 ± 0.015

2.176 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski