Microbacterium phage BeeBee8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ilzatvirus; unclassified Ilzatvirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R3ZZG4|A0A2R3ZZG4_9CAUD Uncharacterized protein OS=Microbacterium phage BeeBee8 OX=2126924 GN=64 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.75LNRR5 pKa = 11.84HH6 pKa = 5.62NLPVPTFGPLVPWGDD21 pKa = 3.68PEE23 pKa = 4.15MEE25 pKa = 4.44PFQTPAWVEE34 pKa = 3.47IEE36 pKa = 3.95GRR38 pKa = 11.84RR39 pKa = 11.84FTWDD43 pKa = 3.75PEE45 pKa = 4.36SGSYY49 pKa = 9.29PGLYY53 pKa = 9.44IEE55 pKa = 5.78PGLNDD60 pKa = 3.33MAQSLTADD68 pKa = 3.35SYY70 pKa = 11.82YY71 pKa = 10.55EE72 pKa = 3.97IEE74 pKa = 4.09EE75 pKa = 4.37DD76 pKa = 3.62

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R3ZZF2|A0A2R3ZZF2_9CAUD Uncharacterized protein OS=Microbacterium phage BeeBee8 OX=2126924 GN=55 PE=4 SV=1
MM1 pKa = 7.37NPLEE5 pKa = 5.78ASDD8 pKa = 3.36PWEE11 pKa = 4.11WLWVMMLWVFWILALVGALILVFAILVGFWRR42 pKa = 11.84AIRR45 pKa = 11.84GILPQVSGLRR55 pKa = 11.84RR56 pKa = 11.84AQVQDD61 pKa = 3.31EE62 pKa = 4.34AAGVAEE68 pKa = 4.5SLYY71 pKa = 10.65GSQFSGSGMAFMAGVDD87 pKa = 3.56YY88 pKa = 10.71VIRR91 pKa = 11.84AFEE94 pKa = 4.12RR95 pKa = 11.84KK96 pKa = 8.93PYY98 pKa = 10.39AGRR101 pKa = 11.84KK102 pKa = 8.58RR103 pKa = 11.84KK104 pKa = 8.43RR105 pKa = 11.84TQPTKK110 pKa = 10.89LII112 pKa = 4.16

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13227

38

807

206.7

22.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.927 ± 0.354

0.59 ± 0.082

6.608 ± 0.279

6.215 ± 0.456

3.19 ± 0.158

7.681 ± 0.307

1.913 ± 0.164

5.149 ± 0.345

4.854 ± 0.361

8.468 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.144

3.387 ± 0.194

4.703 ± 0.206

4.037 ± 0.191

5.678 ± 0.329

5.799 ± 0.261

6.638 ± 0.32

7.477 ± 0.25

2.14 ± 0.222

3.16 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski