Parabacteroides sp. AM08-6

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; unclassified Parabacteroides

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A415DML6|A0A415DML6_9BACT Gliding motility protein GldM OS=Parabacteroides sp. AM08-6 OX=2292053 GN=gldM PE=4 SV=1
MM1 pKa = 6.9EE2 pKa = 5.64AKK4 pKa = 10.07VLSEE8 pKa = 3.7AKK10 pKa = 10.4VYY12 pKa = 10.67VGTYY16 pKa = 9.51GKK18 pKa = 10.39YY19 pKa = 10.34NNGSLFGAWLDD30 pKa = 3.89LSDD33 pKa = 4.65YY34 pKa = 10.83SDD36 pKa = 3.73KK37 pKa = 11.66EE38 pKa = 4.09EE39 pKa = 4.45FYY41 pKa = 9.88EE42 pKa = 4.99ACRR45 pKa = 11.84EE46 pKa = 4.05LHH48 pKa = 6.63KK49 pKa = 11.13DD50 pKa = 3.63EE51 pKa = 5.18EE52 pKa = 4.44DD53 pKa = 3.48AEE55 pKa = 4.74YY56 pKa = 10.09MFQDD60 pKa = 3.69WEE62 pKa = 4.32NVPEE66 pKa = 4.11GLIGEE71 pKa = 4.39SWISEE76 pKa = 3.98NFFALRR82 pKa = 11.84DD83 pKa = 3.82AVEE86 pKa = 4.94DD87 pKa = 4.26LDD89 pKa = 4.64DD90 pKa = 4.21TEE92 pKa = 4.6QEE94 pKa = 5.44AFFVWCNYY102 pKa = 9.34KK103 pKa = 10.33SHH105 pKa = 7.4DD106 pKa = 4.5LSEE109 pKa = 4.34EE110 pKa = 4.03DD111 pKa = 4.79ADD113 pKa = 4.58DD114 pKa = 3.82LVRR117 pKa = 11.84DD118 pKa = 3.98FRR120 pKa = 11.84DD121 pKa = 3.69EE122 pKa = 3.95YY123 pKa = 10.73RR124 pKa = 11.84GQYY127 pKa = 10.56DD128 pKa = 3.55DD129 pKa = 5.61EE130 pKa = 5.06EE131 pKa = 5.71DD132 pKa = 3.22FAYY135 pKa = 10.08EE136 pKa = 4.79IIEE139 pKa = 4.02EE140 pKa = 4.7CYY142 pKa = 10.69NLPEE146 pKa = 3.93FAKK149 pKa = 10.16TYY151 pKa = 10.62FDD153 pKa = 3.75YY154 pKa = 11.44EE155 pKa = 4.0KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 5.04LFMCDD165 pKa = 2.54YY166 pKa = 9.94WFDD169 pKa = 3.78DD170 pKa = 3.9GFVFRR175 pKa = 11.84AAA177 pKa = 3.87

Molecular weight: 21.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R6F9Y3|A0A3R6F9Y3_9BACT MFS transporter OS=Parabacteroides sp. AM08-6 OX=2292053 GN=DW103_03420 PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 4.98VFTEE6 pKa = 4.37LTPEE10 pKa = 4.16CDD12 pKa = 2.67ITAQMYY18 pKa = 9.38AAGYY22 pKa = 6.87EE23 pKa = 4.15KK24 pKa = 11.01KK25 pKa = 10.42EE26 pKa = 3.84IAVLKK31 pKa = 9.92HH32 pKa = 5.77RR33 pKa = 11.84AVSTINNQLQTAFLILGVRR52 pKa = 11.84NGRR55 pKa = 11.84EE56 pKa = 3.81LALKK60 pKa = 10.07LAEE63 pKa = 4.18RR64 pKa = 11.84LSGIRR69 pKa = 11.84LTLDD73 pKa = 3.24FTSATKK79 pKa = 10.58SAVACVLLVILCLNTHH95 pKa = 6.24FDD97 pKa = 3.3MRR99 pKa = 11.84RR100 pKa = 11.84QRR102 pKa = 11.84IRR104 pKa = 11.84PRR106 pKa = 11.84SSANVEE112 pKa = 3.78LVARR116 pKa = 11.84TRR118 pKa = 11.84IRR120 pKa = 11.84ARR122 pKa = 11.84GRR124 pKa = 11.84NILIFF129 pKa = 4.04

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3426

0

3426

1241278

26

3238

362.3

40.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.882 ± 0.044

1.185 ± 0.015

5.488 ± 0.027

6.683 ± 0.038

4.715 ± 0.033

6.739 ± 0.04

1.773 ± 0.017

7.2 ± 0.04

6.886 ± 0.034

9.207 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.02

5.173 ± 0.034

3.823 ± 0.023

3.375 ± 0.02

4.431 ± 0.027

6.124 ± 0.031

5.604 ± 0.036

6.383 ± 0.029

1.215 ± 0.015

4.457 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski