Amino acid dipepetide frequency for Lactobacillus sharpeae JCM 1186 = DSM 20505

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.439AlaAla: 12.439 ± 0.224	0.467AlaCys: 0.467 ± 0.026	6.76AlaAsp: 6.76 ± 0.107	5.359AlaGlu: 5.359 ± 0.106	3.453AlaPhe: 3.453 ± 0.076	7.861AlaGly: 7.861 ± 0.132	2.067AlaHis: 2.067 ± 0.065	6.326AlaIle: 6.326 ± 0.105	6.365AlaLys: 6.365 ± 0.13	9.749AlaLeu: 9.749 ± 0.14	2.782AlaMet: 2.782 ± 0.061	4.145AlaAsn: 4.145 ± 0.079	3.366AlaPro: 3.366 ± 0.09	4.534AlaGln: 4.534 ± 0.099	4.345AlaArg: 4.345 ± 0.096	5.034AlaSer: 5.034 ± 0.084	7.091AlaThr: 7.091 ± 0.123	7.688AlaVal: 7.688 ± 0.106	0.902AlaTrp: 0.902 ± 0.036	2.763AlaTyr: 2.763 ± 0.076	0.0AlaXaa: 0.0 ± 0.0
Cys	0.466CysAla: 0.466 ± 0.029	0.044CysCys: 0.044 ± 0.008	0.21CysAsp: 0.21 ± 0.019	0.177CysGlu: 0.177 ± 0.017	0.189CysPhe: 0.189 ± 0.015	0.495CysGly: 0.495 ± 0.027	0.117CysHis: 0.117 ± 0.014	0.261CysIle: 0.261 ± 0.022	0.157CysLys: 0.157 ± 0.015	0.444CysLeu: 0.444 ± 0.023	0.088CysMet: 0.088 ± 0.012	0.149CysAsn: 0.149 ± 0.014	0.183CysPro: 0.183 ± 0.019	0.128CysGln: 0.128 ± 0.014	0.187CysArg: 0.187 ± 0.017	0.255CysSer: 0.255 ± 0.019	0.283CysThr: 0.283 ± 0.022	0.303CysVal: 0.303 ± 0.026	0.051CysTrp: 0.051 ± 0.008	0.136CysTyr: 0.136 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.429AspAla: 6.429 ± 0.107	0.222AspCys: 0.222 ± 0.019	3.848AspAsp: 3.848 ± 0.116	3.717AspGlu: 3.717 ± 0.087	2.631AspPhe: 2.631 ± 0.067	4.471AspGly: 4.471 ± 0.13	1.334AspHis: 1.334 ± 0.049	3.501AspIle: 3.501 ± 0.079	3.124AspLys: 3.124 ± 0.083	5.397AspLeu: 5.397 ± 0.103	1.652AspMet: 1.652 ± 0.049	2.528AspAsn: 2.528 ± 0.081	2.289AspPro: 2.289 ± 0.065	2.458AspGln: 2.458 ± 0.062	2.402AspArg: 2.402 ± 0.069	2.722AspSer: 2.722 ± 0.075	3.469AspThr: 3.469 ± 0.074	4.47AspVal: 4.47 ± 0.08	0.744AspTrp: 0.744 ± 0.034	2.463AspTyr: 2.463 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	4.518GluAla: 4.518 ± 0.093	0.197GluCys: 0.197 ± 0.018	2.718GluAsp: 2.718 ± 0.068	2.641GluGlu: 2.641 ± 0.082	2.165GluPhe: 2.165 ± 0.05	2.386GluGly: 2.386 ± 0.059	1.24GluHis: 1.24 ± 0.046	3.112GluIle: 3.112 ± 0.066	2.612GluLys: 2.612 ± 0.067	5.821GluLeu: 5.821 ± 0.108	1.555GluMet: 1.555 ± 0.047	2.039GluAsn: 2.039 ± 0.053	1.882GluPro: 1.882 ± 0.057	2.497GluGln: 2.497 ± 0.063	2.689GluArg: 2.689 ± 0.067	2.216GluSer: 2.216 ± 0.057	2.848GluThr: 2.848 ± 0.069	3.37GluVal: 3.37 ± 0.082	0.458GluTrp: 0.458 ± 0.028	1.858GluTyr: 1.858 ± 0.056	0.001GluXaa: 0.001 ± 0.002
Phe	4.419PheAla: 4.419 ± 0.073	0.254PheCys: 0.254 ± 0.02	2.547PheAsp: 2.547 ± 0.072	1.662PheGlu: 1.662 ± 0.047	1.652PhePhe: 1.652 ± 0.051	3.427PheGly: 3.427 ± 0.085	0.727PheHis: 0.727 ± 0.032	2.548PheIle: 2.548 ± 0.078	1.916PheLys: 1.916 ± 0.049	3.312PheLeu: 3.312 ± 0.078	1.178PheMet: 1.178 ± 0.043	1.848PheAsn: 1.848 ± 0.051	1.347PhePro: 1.347 ± 0.047	1.154PheGln: 1.154 ± 0.039	1.507PheArg: 1.507 ± 0.05	2.431PheSer: 2.431 ± 0.059	3.03PheThr: 3.03 ± 0.071	3.04PheVal: 3.04 ± 0.083	0.582PheTrp: 0.582 ± 0.033	1.326PheTyr: 1.326 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	6.42GlyAla: 6.42 ± 0.112	0.326GlyCys: 0.326 ± 0.024	3.935GlyAsp: 3.935 ± 0.09	3.157GlyGlu: 3.157 ± 0.077	3.027GlyPhe: 3.027 ± 0.068	4.914GlyGly: 4.914 ± 0.131	1.527GlyHis: 1.527 ± 0.056	5.091GlyIle: 5.091 ± 0.104	4.422GlyLys: 4.422 ± 0.083	7.086GlyLeu: 7.086 ± 0.116	2.206GlyMet: 2.206 ± 0.067	2.911GlyAsn: 2.911 ± 0.09	1.642GlyPro: 1.642 ± 0.054	3.12GlyGln: 3.12 ± 0.082	3.028GlyArg: 3.028 ± 0.073	3.99GlySer: 3.99 ± 0.097	4.977GlyThr: 4.977 ± 0.107	5.663GlyVal: 5.663 ± 0.103	0.803GlyTrp: 0.803 ± 0.038	2.731GlyTyr: 2.731 ± 0.079	0.001GlyXaa: 0.001 ± 0.001
His	2.088HisAla: 2.088 ± 0.063	0.09HisCys: 0.09 ± 0.012	1.336HisAsp: 1.336 ± 0.045	1.144HisGlu: 1.144 ± 0.041	1.014HisPhe: 1.014 ± 0.043	1.611HisGly: 1.611 ± 0.054	0.644HisHis: 0.644 ± 0.027	1.295HisIle: 1.295 ± 0.042	1.001HisLys: 1.001 ± 0.036	2.122HisLeu: 2.122 ± 0.056	0.576HisMet: 0.576 ± 0.028	0.934HisAsn: 0.934 ± 0.036	1.076HisPro: 1.076 ± 0.047	0.937HisGln: 0.937 ± 0.039	1.025HisArg: 1.025 ± 0.035	1.012HisSer: 1.012 ± 0.046	1.375HisThr: 1.375 ± 0.043	1.756HisVal: 1.756 ± 0.048	0.277HisTrp: 0.277 ± 0.021	0.908HisTyr: 0.908 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	6.74IleAla: 6.74 ± 0.117	0.365IleCys: 0.365 ± 0.022	4.187IleAsp: 4.187 ± 0.085	2.915IleGlu: 2.915 ± 0.062	2.495IlePhe: 2.495 ± 0.072	4.883IleGly: 4.883 ± 0.105	1.207IleHis: 1.207 ± 0.042	4.274IleIle: 4.274 ± 0.104	3.137IleLys: 3.137 ± 0.07	5.214IleLeu: 5.214 ± 0.111	1.82IleMet: 1.82 ± 0.051	2.946IleAsn: 2.946 ± 0.062	2.403IlePro: 2.403 ± 0.066	1.98IleGln: 1.98 ± 0.052	2.521IleArg: 2.521 ± 0.063	3.647IleSer: 3.647 ± 0.089	4.328IleThr: 4.328 ± 0.094	5.053IleVal: 5.053 ± 0.097	0.58IleTrp: 0.58 ± 0.03	1.706IleTyr: 1.706 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.825LysAla: 4.825 ± 0.099	0.139LysCys: 0.139 ± 0.015	2.933LysAsp: 2.933 ± 0.079	2.557LysGlu: 2.557 ± 0.074	1.748LysPhe: 1.748 ± 0.059	2.69LysGly: 2.69 ± 0.069	1.308LysHis: 1.308 ± 0.04	3.092LysIle: 3.092 ± 0.069	3.417LysLys: 3.417 ± 0.104	5.076LysLeu: 5.076 ± 0.098	1.849LysMet: 1.849 ± 0.056	2.435LysAsn: 2.435 ± 0.066	2.093LysPro: 2.093 ± 0.053	3.053LysGln: 3.053 ± 0.075	2.867LysArg: 2.867 ± 0.074	2.89LysSer: 2.89 ± 0.08	3.707LysThr: 3.707 ± 0.09	3.514LysVal: 3.514 ± 0.073	0.525LysTrp: 0.525 ± 0.029	2.0LysTyr: 2.0 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	10.69LeuAla: 10.69 ± 0.158	0.483LeuCys: 0.483 ± 0.032	5.534LeuAsp: 5.534 ± 0.087	4.054LeuGlu: 4.054 ± 0.08	3.766LeuPhe: 3.766 ± 0.094	6.911LeuGly: 6.911 ± 0.118	2.206LeuHis: 2.206 ± 0.062	6.096LeuIle: 6.096 ± 0.095	4.361LeuLys: 4.361 ± 0.082	9.507LeuLeu: 9.507 ± 0.168	2.522LeuMet: 2.522 ± 0.077	4.213LeuAsn: 4.213 ± 0.079	4.497LeuPro: 4.497 ± 0.091	3.967LeuGln: 3.967 ± 0.089	4.773LeuArg: 4.773 ± 0.092	5.472LeuSer: 5.472 ± 0.093	7.56LeuThr: 7.56 ± 0.126	7.04LeuVal: 7.04 ± 0.135	0.825LeuTrp: 0.825 ± 0.036	2.597LeuTyr: 2.597 ± 0.069	0.001LeuXaa: 0.001 ± 0.001
Met	2.792MetAla: 2.792 ± 0.065	0.125MetCys: 0.125 ± 0.013	1.411MetAsp: 1.411 ± 0.047	1.098MetGlu: 1.098 ± 0.045	1.062MetPhe: 1.062 ± 0.034	1.788MetGly: 1.788 ± 0.053	0.683MetHis: 0.683 ± 0.033	1.603MetIle: 1.603 ± 0.063	1.33MetLys: 1.33 ± 0.047	2.859MetLeu: 2.859 ± 0.074	0.864MetMet: 0.864 ± 0.035	1.371MetAsn: 1.371 ± 0.044	1.249MetPro: 1.249 ± 0.042	1.382MetGln: 1.382 ± 0.044	1.51MetArg: 1.51 ± 0.045	1.568MetSer: 1.568 ± 0.052	2.152MetThr: 2.152 ± 0.061	1.98MetVal: 1.98 ± 0.058	0.235MetTrp: 0.235 ± 0.019	0.725MetTyr: 0.725 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	4.138AsnAla: 4.138 ± 0.075	0.157AsnCys: 0.157 ± 0.015	2.316AsnAsp: 2.316 ± 0.069	2.088AsnGlu: 2.088 ± 0.068	1.736AsnPhe: 1.736 ± 0.051	3.742AsnGly: 3.742 ± 0.105	1.036AsnHis: 1.036 ± 0.037	2.529AsnIle: 2.529 ± 0.067	2.202AsnLys: 2.202 ± 0.066	3.997AsnLeu: 3.997 ± 0.079	1.189AsnMet: 1.189 ± 0.04	1.769AsnAsn: 1.769 ± 0.069	2.068AsnPro: 2.068 ± 0.062	1.994AsnGln: 1.994 ± 0.052	1.864AsnArg: 1.864 ± 0.054	2.148AsnSer: 2.148 ± 0.052	2.496AsnThr: 2.496 ± 0.07	3.046AsnVal: 3.046 ± 0.07	0.553AsnTrp: 0.553 ± 0.031	1.52AsnTyr: 1.52 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	4.336ProAla: 4.336 ± 0.092	0.093ProCys: 0.093 ± 0.012	2.914ProAsp: 2.914 ± 0.078	2.856ProGlu: 2.856 ± 0.077	1.604ProPhe: 1.604 ± 0.052	2.542ProGly: 2.542 ± 0.072	0.769ProHis: 0.769 ± 0.035	2.132ProIle: 2.132 ± 0.06	1.871ProLys: 1.871 ± 0.058	3.587ProLeu: 3.587 ± 0.085	0.79ProMet: 0.79 ± 0.033	1.507ProAsn: 1.507 ± 0.051	0.645ProPro: 0.645 ± 0.033	1.619ProGln: 1.619 ± 0.048	1.429ProArg: 1.429 ± 0.047	1.793ProSer: 1.793 ± 0.062	2.583ProThr: 2.583 ± 0.076	3.228ProVal: 3.228 ± 0.076	0.347ProTrp: 0.347 ± 0.025	1.194ProTyr: 1.194 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.255GlnAla: 4.255 ± 0.091	0.128GlnCys: 0.128 ± 0.014	2.11GlnAsp: 2.11 ± 0.055	2.067GlnGlu: 2.067 ± 0.057	1.752GlnPhe: 1.752 ± 0.053	2.186GlnGly: 2.186 ± 0.05	1.067GlnHis: 1.067 ± 0.041	2.776GlnIle: 2.776 ± 0.065	2.286GlnLys: 2.286 ± 0.06	4.96GlnLeu: 4.96 ± 0.115	1.297GlnMet: 1.297 ± 0.048	1.914GlnAsn: 1.914 ± 0.048	1.601GlnPro: 1.601 ± 0.053	2.438GlnGln: 2.438 ± 0.072	2.197GlnArg: 2.197 ± 0.067	2.12GlnSer: 2.12 ± 0.053	2.816GlnThr: 2.816 ± 0.075	3.346GlnVal: 3.346 ± 0.069	0.426GlnTrp: 0.426 ± 0.023	1.565GlnTyr: 1.565 ± 0.056	0.0GlnXaa: 0.0 ± 0.0
Arg	4.246ArgAla: 4.246 ± 0.092	0.16ArgCys: 0.16 ± 0.015	2.718ArgAsp: 2.718 ± 0.071	2.634ArgGlu: 2.634 ± 0.069	1.914ArgPhe: 1.914 ± 0.058	2.988ArgGly: 2.988 ± 0.072	1.136ArgHis: 1.136 ± 0.04	2.921ArgIle: 2.921 ± 0.071	2.637ArgLys: 2.637 ± 0.065	4.403ArgLeu: 4.403 ± 0.088	1.394ArgMet: 1.394 ± 0.053	1.946ArgAsn: 1.946 ± 0.052	1.652ArgPro: 1.652 ± 0.058	2.203ArgGln: 2.203 ± 0.058	2.767ArgArg: 2.767 ± 0.076	2.175ArgSer: 2.175 ± 0.053	2.735ArgThr: 2.735 ± 0.072	3.304ArgVal: 3.304 ± 0.077	0.466ArgTrp: 0.466 ± 0.031	1.675ArgTyr: 1.675 ± 0.059	0.0ArgXaa: 0.0 ± 0.0
Ser	5.328SerAla: 5.328 ± 0.111	0.189SerCys: 0.189 ± 0.017	3.233SerAsp: 3.233 ± 0.077	2.521SerGlu: 2.521 ± 0.07	2.348SerPhe: 2.348 ± 0.06	4.648SerGly: 4.648 ± 0.094	1.041SerHis: 1.041 ± 0.034	3.223SerIle: 3.223 ± 0.081	2.988SerLys: 2.988 ± 0.082	4.861SerLeu: 4.861 ± 0.093	1.447SerMet: 1.447 ± 0.049	2.152SerAsn: 2.152 ± 0.058	1.629SerPro: 1.629 ± 0.051	1.952SerGln: 1.952 ± 0.056	2.358SerArg: 2.358 ± 0.063	3.415SerSer: 3.415 ± 0.132	3.571SerThr: 3.571 ± 0.082	3.994SerVal: 3.994 ± 0.075	0.619SerTrp: 0.619 ± 0.032	1.777SerTyr: 1.777 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	7.099ThrAla: 7.099 ± 0.131	0.229ThrCys: 0.229 ± 0.02	4.294ThrAsp: 4.294 ± 0.123	3.115ThrGlu: 3.115 ± 0.077	2.577ThrPhe: 2.577 ± 0.061	5.166ThrGly: 5.166 ± 0.085	1.346ThrHis: 1.346 ± 0.047	4.545ThrIle: 4.545 ± 0.089	3.672ThrLys: 3.672 ± 0.091	6.236ThrLeu: 6.236 ± 0.095	1.537ThrMet: 1.537 ± 0.047	2.805ThrAsn: 2.805 ± 0.075	3.336ThrPro: 3.336 ± 0.115	2.503ThrGln: 2.503 ± 0.068	2.566ThrArg: 2.566 ± 0.059	3.737ThrSer: 3.737 ± 0.09	4.918ThrThr: 4.918 ± 0.154	5.9ThrVal: 5.9 ± 0.125	0.689ThrTrp: 0.689 ± 0.038	2.055ThrTyr: 2.055 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	8.301ValAla: 8.301 ± 0.126	0.419ValCys: 0.419 ± 0.026	4.489ValAsp: 4.489 ± 0.092	3.343ValGlu: 3.343 ± 0.079	2.699ValPhe: 2.699 ± 0.058	5.211ValGly: 5.211 ± 0.098	1.601ValHis: 1.601 ± 0.049	4.703ValIle: 4.703 ± 0.094	3.446ValLys: 3.446 ± 0.078	7.611ValLeu: 7.611 ± 0.133	1.867ValMet: 1.867 ± 0.052	3.178ValAsn: 3.178 ± 0.068	3.234ValPro: 3.234 ± 0.085	3.181ValGln: 3.181 ± 0.079	3.591ValArg: 3.591 ± 0.088	4.313ValSer: 4.313 ± 0.089	5.547ValThr: 5.547 ± 0.112	5.903ValVal: 5.903 ± 0.108	0.67ValTrp: 0.67 ± 0.032	2.278ValTyr: 2.278 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.802TrpAla: 0.802 ± 0.035	0.074TrpCys: 0.074 ± 0.01	0.441TrpAsp: 0.441 ± 0.023	0.363TrpGlu: 0.363 ± 0.023	0.554TrpPhe: 0.554 ± 0.028	0.677TrpGly: 0.677 ± 0.037	0.37TrpHis: 0.37 ± 0.026	0.576TrpIle: 0.576 ± 0.029	0.297TrpLys: 0.297 ± 0.023	1.378TrpLeu: 1.378 ± 0.056	0.306TrpMet: 0.306 ± 0.023	0.421TrpAsn: 0.421 ± 0.023	0.361TrpPro: 0.361 ± 0.025	0.695TrpGln: 0.695 ± 0.037	0.692TrpArg: 0.692 ± 0.031	0.548TrpSer: 0.548 ± 0.031	0.599TrpThr: 0.599 ± 0.032	0.693TrpVal: 0.693 ± 0.035	0.196TrpTrp: 0.196 ± 0.017	0.338TrpTyr: 0.338 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.159TyrAla: 3.159 ± 0.06	0.162TyrCys: 0.162 ± 0.017	1.938TyrAsp: 1.938 ± 0.056	1.359TyrGlu: 1.359 ± 0.045	1.52TyrPhe: 1.52 ± 0.044	2.381TyrGly: 2.381 ± 0.062	0.796TyrHis: 0.796 ± 0.035	1.797TyrIle: 1.797 ± 0.054	1.479TyrLys: 1.479 ± 0.052	3.455TyrLeu: 3.455 ± 0.082	0.864TyrMet: 0.864 ± 0.034	1.376TyrAsn: 1.376 ± 0.046	1.284TyrPro: 1.284 ± 0.049	1.514TyrGln: 1.514 ± 0.047	1.756TyrArg: 1.756 ± 0.058	1.806TyrSer: 1.806 ± 0.057	2.241TyrThr: 2.241 ± 0.066	2.294TyrVal: 2.294 ± 0.059	0.432TyrTrp: 0.432 ± 0.023	1.282TyrTyr: 1.282 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.117XaaXaa: 0.117 ± 0.075

Statistics based on 2271 proteins (689515 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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