Amino acid dipepetide frequency for Methanobrevibacter ruminantium (strain ATCC 35063 / DSM 1093 / JCM 13430 / OCM 146 / M1) (Methanobacterium ruminantium)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.59AlaAla: 3.59 ± 0.116
0.729AlaCys: 0.729 ± 0.038
3.646AlaAsp: 3.646 ± 0.087
3.882AlaGlu: 3.882 ± 0.097
2.478AlaPhe: 2.478 ± 0.064
3.952AlaGly: 3.952 ± 0.09
0.881AlaHis: 0.881 ± 0.038
6.541AlaIle: 6.541 ± 0.129
4.569AlaLys: 4.569 ± 0.103
5.392AlaLeu: 5.392 ± 0.109
1.438AlaMet: 1.438 ± 0.049
3.718AlaAsn: 3.718 ± 0.142
1.736AlaPro: 1.736 ± 0.05
1.472AlaGln: 1.472 ± 0.053
1.959AlaArg: 1.959 ± 0.061
4.331AlaSer: 4.331 ± 0.109
3.264AlaThr: 3.264 ± 0.138
4.117AlaVal: 4.117 ± 0.1
0.337AlaTrp: 0.337 ± 0.025
2.322AlaTyr: 2.322 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.601CysAla: 0.601 ± 0.032
0.18CysCys: 0.18 ± 0.017
0.81CysAsp: 0.81 ± 0.033
0.793CysGlu: 0.793 ± 0.036
0.489CysPhe: 0.489 ± 0.027
1.268CysGly: 1.268 ± 0.053
0.237CysHis: 0.237 ± 0.02
0.859CysIle: 0.859 ± 0.036
0.721CysLys: 0.721 ± 0.035
0.934CysLeu: 0.934 ± 0.042
0.243CysMet: 0.243 ± 0.018
0.621CysAsn: 0.621 ± 0.044
0.749CysPro: 0.749 ± 0.042
0.259CysGln: 0.259 ± 0.017
0.359CysArg: 0.359 ± 0.026
0.854CysSer: 0.854 ± 0.04
0.584CysThr: 0.584 ± 0.031
0.638CysVal: 0.638 ± 0.033
0.082CysTrp: 0.082 ± 0.009
0.422CysTyr: 0.422 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
3.782AspAla: 3.782 ± 0.086
0.948AspCys: 0.948 ± 0.047
5.314AspAsp: 5.314 ± 0.135
6.221AspGlu: 6.221 ± 0.135
3.366AspPhe: 3.366 ± 0.081
4.779AspGly: 4.779 ± 0.138
0.843AspHis: 0.843 ± 0.056
6.239AspIle: 6.239 ± 0.094
4.844AspLys: 4.844 ± 0.107
6.721AspLeu: 6.721 ± 0.122
1.694AspMet: 1.694 ± 0.045
4.554AspAsn: 4.554 ± 0.142
1.765AspPro: 1.765 ± 0.052
0.998AspGln: 0.998 ± 0.045
1.654AspArg: 1.654 ± 0.05
5.184AspSer: 5.184 ± 0.114
2.694AspThr: 2.694 ± 0.071
4.313AspVal: 4.313 ± 0.105
0.53AspTrp: 0.53 ± 0.031
3.516AspTyr: 3.516 ± 0.079
0.0AspXaa: 0.0 ± 0.0
Glu
4.182GluAla: 4.182 ± 0.118
0.631GluCys: 0.631 ± 0.032
5.81GluAsp: 5.81 ± 0.134
7.732GluGlu: 7.732 ± 0.202
3.383GluPhe: 3.383 ± 0.09
4.45GluGly: 4.45 ± 0.095
1.048GluHis: 1.048 ± 0.056
7.263GluIle: 7.263 ± 0.14
5.809GluLys: 5.809 ± 0.149
6.811GluLeu: 6.811 ± 0.142
1.893GluMet: 1.893 ± 0.068
5.571GluAsn: 5.571 ± 0.116
1.54GluPro: 1.54 ± 0.054
1.271GluGln: 1.271 ± 0.047
2.503GluArg: 2.503 ± 0.07
4.563GluSer: 4.563 ± 0.095
3.374GluThr: 3.374 ± 0.093
4.321GluVal: 4.321 ± 0.091
0.484GluTrp: 0.484 ± 0.028
3.231GluTyr: 3.231 ± 0.078
0.0GluXaa: 0.0 ± 0.0
Phe
2.569PheAla: 2.569 ± 0.072
0.52PheCys: 0.52 ± 0.032
3.441PheAsp: 3.441 ± 0.083
3.311PheGlu: 3.311 ± 0.073
1.834PhePhe: 1.834 ± 0.062
2.715PheGly: 2.715 ± 0.07
0.668PheHis: 0.668 ± 0.035
4.149PheIle: 4.149 ± 0.083
3.491PheLys: 3.491 ± 0.1
3.941PheLeu: 3.941 ± 0.101
0.908PheMet: 0.908 ± 0.034
3.352PheAsn: 3.352 ± 0.089
1.148PhePro: 1.148 ± 0.045
0.784PheGln: 0.784 ± 0.031
1.247PheArg: 1.247 ± 0.049
2.924PheSer: 2.924 ± 0.085
2.616PheThr: 2.616 ± 0.108
2.585PheVal: 2.585 ± 0.075
0.267PheTrp: 0.267 ± 0.021
1.763PheTyr: 1.763 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.92GlyAla: 4.92 ± 0.158
0.875GlyCys: 0.875 ± 0.04
4.311GlyAsp: 4.311 ± 0.122
4.505GlyGlu: 4.505 ± 0.093
3.049GlyPhe: 3.049 ± 0.079
4.999GlyGly: 4.999 ± 0.171
1.026GlyHis: 1.026 ± 0.04
5.834GlyIle: 5.834 ± 0.127
4.653GlyLys: 4.653 ± 0.084
5.414GlyLeu: 5.414 ± 0.1
1.505GlyMet: 1.505 ± 0.055
4.024GlyAsn: 4.024 ± 0.164
1.547GlyPro: 1.547 ± 0.058
1.509GlyGln: 1.509 ± 0.041
2.106GlyArg: 2.106 ± 0.055
4.586GlySer: 4.586 ± 0.121
3.937GlyThr: 3.937 ± 0.161
4.265GlyVal: 4.265 ± 0.091
0.474GlyTrp: 0.474 ± 0.028
2.482GlyTyr: 2.482 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
0.81HisAla: 0.81 ± 0.037
0.263HisCys: 0.263 ± 0.019
0.93HisAsp: 0.93 ± 0.049
1.001HisGlu: 1.001 ± 0.054
0.67HisPhe: 0.67 ± 0.033
1.055HisGly: 1.055 ± 0.046
0.38HisHis: 0.38 ± 0.034
1.218HisIle: 1.218 ± 0.044
0.926HisLys: 0.926 ± 0.047
1.198HisLeu: 1.198 ± 0.043
0.389HisMet: 0.389 ± 0.023
0.868HisAsn: 0.868 ± 0.038
0.68HisPro: 0.68 ± 0.03
0.3HisGln: 0.3 ± 0.02
0.566HisArg: 0.566 ± 0.032
1.008HisSer: 1.008 ± 0.045
0.662HisThr: 0.662 ± 0.033
0.833HisVal: 0.833 ± 0.035
0.11HisTrp: 0.11 ± 0.012
0.708HisTyr: 0.708 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.853IleAla: 5.853 ± 0.124
1.185IleCys: 1.185 ± 0.05
6.485IleAsp: 6.485 ± 0.116
6.763IleGlu: 6.763 ± 0.138
4.029IlePhe: 4.029 ± 0.098
5.679IleGly: 5.679 ± 0.119
1.334IleHis: 1.334 ± 0.047
7.986IleIle: 7.986 ± 0.139
6.43IleLys: 6.43 ± 0.117
8.094IleLeu: 8.094 ± 0.158
1.775IleMet: 1.775 ± 0.061
5.708IleAsn: 5.708 ± 0.145
3.105IlePro: 3.105 ± 0.079
1.872IleGln: 1.872 ± 0.055
2.924IleArg: 2.924 ± 0.074
6.79IleSer: 6.79 ± 0.108
4.815IleThr: 4.815 ± 0.169
5.916IleVal: 5.916 ± 0.103
0.539IleTrp: 0.539 ± 0.032
3.514IleTyr: 3.514 ± 0.1
0.0IleXaa: 0.0 ± 0.0
Lys
4.317LysAla: 4.317 ± 0.088
0.652LysCys: 0.652 ± 0.035
5.338LysAsp: 5.338 ± 0.114
7.017LysGlu: 7.017 ± 0.193
2.701LysPhe: 2.701 ± 0.07
4.211LysGly: 4.211 ± 0.083
0.956LysHis: 0.956 ± 0.043
6.551LysIle: 6.551 ± 0.129
5.741LysLys: 5.741 ± 0.163
5.791LysLeu: 5.791 ± 0.138
1.726LysMet: 1.726 ± 0.054
4.628LysAsn: 4.628 ± 0.118
1.936LysPro: 1.936 ± 0.066
1.597LysGln: 1.597 ± 0.053
2.787LysArg: 2.787 ± 0.084
4.75LysSer: 4.75 ± 0.118
4.283LysThr: 4.283 ± 0.103
4.612LysVal: 4.612 ± 0.093
0.503LysTrp: 0.503 ± 0.04
3.172LysTyr: 3.172 ± 0.091
0.0LysXaa: 0.0 ± 0.0
Leu
5.791LeuAla: 5.791 ± 0.119
0.825LeuCys: 0.825 ± 0.04
6.523LeuAsp: 6.523 ± 0.106
6.572LeuGlu: 6.572 ± 0.127
3.766LeuPhe: 3.766 ± 0.093
5.522LeuGly: 5.522 ± 0.11
1.03LeuHis: 1.03 ± 0.038
7.809LeuIle: 7.809 ± 0.153
7.272LeuLys: 7.272 ± 0.181
6.671LeuLeu: 6.671 ± 0.145
2.009LeuMet: 2.009 ± 0.062
6.352LeuAsn: 6.352 ± 0.135
2.509LeuPro: 2.509 ± 0.065
1.543LeuGln: 1.543 ± 0.05
2.774LeuArg: 2.774 ± 0.073
6.472LeuSer: 6.472 ± 0.122
4.679LeuThr: 4.679 ± 0.11
5.058LeuVal: 5.058 ± 0.101
0.434LeuTrp: 0.434 ± 0.025
2.906LeuTyr: 2.906 ± 0.087
0.0LeuXaa: 0.0 ± 0.0
Met
1.68MetAla: 1.68 ± 0.062
0.23MetCys: 0.23 ± 0.017
1.689MetAsp: 1.689 ± 0.052
1.798MetGlu: 1.798 ± 0.059
0.854MetPhe: 0.854 ± 0.037
1.583MetGly: 1.583 ± 0.059
0.367MetHis: 0.367 ± 0.024
1.907MetIle: 1.907 ± 0.055
1.763MetLys: 1.763 ± 0.061
1.534MetLeu: 1.534 ± 0.052
0.568MetMet: 0.568 ± 0.027
1.367MetAsn: 1.367 ± 0.049
0.726MetPro: 0.726 ± 0.032
0.414MetGln: 0.414 ± 0.023
0.677MetArg: 0.677 ± 0.034
1.442MetSer: 1.442 ± 0.049
1.123MetThr: 1.123 ± 0.046
1.483MetVal: 1.483 ± 0.051
0.13MetTrp: 0.13 ± 0.015
0.655MetTyr: 0.655 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.562AsnAla: 3.562 ± 0.126
0.988AsnCys: 0.988 ± 0.057
4.075AsnAsp: 4.075 ± 0.09
4.559AsnGlu: 4.559 ± 0.1
2.627AsnPhe: 2.627 ± 0.071
4.884AsnGly: 4.884 ± 0.218
1.009AsnHis: 1.009 ± 0.043
5.934AsnIle: 5.934 ± 0.155
4.703AsnLys: 4.703 ± 0.133
5.933AsnLeu: 5.933 ± 0.106
1.368AsnMet: 1.368 ± 0.043
5.187AsnAsn: 5.187 ± 0.258
2.606AsnPro: 2.606 ± 0.076
1.823AsnGln: 1.823 ± 0.071
2.055AsnArg: 2.055 ± 0.056
4.984AsnSer: 4.984 ± 0.176
3.37AsnThr: 3.37 ± 0.164
4.015AsnVal: 4.015 ± 0.149
0.539AsnTrp: 0.539 ± 0.04
3.101AsnTyr: 3.101 ± 0.116
0.0AsnXaa: 0.0 ± 0.0
Pro
1.597ProAla: 1.597 ± 0.053
0.334ProCys: 0.334 ± 0.021
1.952ProAsp: 1.952 ± 0.061
2.514ProGlu: 2.514 ± 0.071
1.35ProPhe: 1.35 ± 0.043
1.835ProGly: 1.835 ± 0.055
0.572ProHis: 0.572 ± 0.031
2.898ProIle: 2.898 ± 0.067
2.173ProLys: 2.173 ± 0.075
2.585ProLeu: 2.585 ± 0.073
0.655ProMet: 0.655 ± 0.033
1.776ProAsn: 1.776 ± 0.055
0.718ProPro: 0.718 ± 0.033
0.756ProGln: 0.756 ± 0.039
0.992ProArg: 0.992 ± 0.042
1.95ProSer: 1.95 ± 0.057
1.479ProThr: 1.479 ± 0.044
2.218ProVal: 2.218 ± 0.06
0.191ProTrp: 0.191 ± 0.017
1.222ProTyr: 1.222 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
1.394GlnAla: 1.394 ± 0.055
0.228GlnCys: 0.228 ± 0.018
1.283GlnAsp: 1.283 ± 0.048
1.592GlnGlu: 1.592 ± 0.057
0.888GlnPhe: 0.888 ± 0.036
1.231GlnGly: 1.231 ± 0.041
0.289GlnHis: 0.289 ± 0.019
2.03GlnIle: 2.03 ± 0.044
1.701GlnLys: 1.701 ± 0.059
1.948GlnLeu: 1.948 ± 0.068
0.599GlnMet: 0.599 ± 0.033
1.243GlnAsn: 1.243 ± 0.038
0.493GlnPro: 0.493 ± 0.025
0.504GlnGln: 0.504 ± 0.034
0.742GlnArg: 0.742 ± 0.029
1.488GlnSer: 1.488 ± 0.05
1.177GlnThr: 1.177 ± 0.044
1.239GlnVal: 1.239 ± 0.038
0.18GlnTrp: 0.18 ± 0.017
0.981GlnTyr: 0.981 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
1.756ArgAla: 1.756 ± 0.052
0.412ArgCys: 0.412 ± 0.024
2.001ArgAsp: 2.001 ± 0.059
2.693ArgGlu: 2.693 ± 0.089
1.504ArgPhe: 1.504 ± 0.052
1.978ArgGly: 1.978 ± 0.069
0.492ArgHis: 0.492 ± 0.027
2.982ArgIle: 2.982 ± 0.073
2.47ArgLys: 2.47 ± 0.071
2.739ArgLeu: 2.739 ± 0.07
0.767ArgMet: 0.767 ± 0.04
2.005ArgAsn: 2.005 ± 0.059
0.992ArgPro: 0.992 ± 0.049
0.821ArgGln: 0.821 ± 0.039
1.472ArgArg: 1.472 ± 0.059
1.719ArgSer: 1.719 ± 0.047
1.61ArgThr: 1.61 ± 0.056
1.946ArgVal: 1.946 ± 0.06
0.228ArgTrp: 0.228 ± 0.02
1.251ArgTyr: 1.251 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
4.021SerAla: 4.021 ± 0.106
0.679SerCys: 0.679 ± 0.032
4.809SerAsp: 4.809 ± 0.109
4.254SerGlu: 4.254 ± 0.084
3.72SerPhe: 3.72 ± 0.084
4.638SerGly: 4.638 ± 0.127
1.119SerHis: 1.119 ± 0.049
5.985SerIle: 5.985 ± 0.105
5.212SerLys: 5.212 ± 0.126
6.245SerLeu: 6.245 ± 0.099
1.289SerMet: 1.289 ± 0.047
5.374SerAsn: 5.374 ± 0.184
2.01SerPro: 2.01 ± 0.057
1.885SerGln: 1.885 ± 0.06
2.176SerArg: 2.176 ± 0.051
6.279SerSer: 6.279 ± 0.212
3.604SerThr: 3.604 ± 0.106
3.995SerVal: 3.995 ± 0.094
0.493SerTrp: 0.493 ± 0.027
3.014SerTyr: 3.014 ± 0.084
0.0SerXaa: 0.0 ± 0.0
Thr
3.412ThrAla: 3.412 ± 0.115
0.521ThrCys: 0.521 ± 0.03
3.516ThrAsp: 3.516 ± 0.117
3.006ThrGlu: 3.006 ± 0.078
2.44ThrPhe: 2.44 ± 0.088
3.857ThrGly: 3.857 ± 0.13
0.713ThrHis: 0.713 ± 0.04
5.392ThrIle: 5.392 ± 0.177
3.173ThrLys: 3.173 ± 0.066
4.619ThrLeu: 4.619 ± 0.112
0.891ThrMet: 0.891 ± 0.035
3.323ThrAsn: 3.323 ± 0.197
1.697ThrPro: 1.697 ± 0.056
1.042ThrGln: 1.042 ± 0.038
1.506ThrArg: 1.506 ± 0.047
3.149ThrSer: 3.149 ± 0.08
2.851ThrThr: 2.851 ± 0.086
4.728ThrVal: 4.728 ± 0.258
0.358ThrTrp: 0.358 ± 0.03
2.221ThrTyr: 2.221 ± 0.079
0.0ThrXaa: 0.0 ± 0.0
Val
3.906ValAla: 3.906 ± 0.098
0.872ValCys: 0.872 ± 0.036
4.35ValAsp: 4.35 ± 0.095
4.486ValGlu: 4.486 ± 0.098
2.815ValPhe: 2.815 ± 0.072
3.898ValGly: 3.898 ± 0.093
0.868ValHis: 0.868 ± 0.037
5.446ValIle: 5.446 ± 0.1
4.455ValLys: 4.455 ± 0.085
5.584ValLeu: 5.584 ± 0.11
1.187ValMet: 1.187 ± 0.044
4.045ValAsn: 4.045 ± 0.151
2.313ValPro: 2.313 ± 0.077
1.264ValGln: 1.264 ± 0.042
1.909ValArg: 1.909 ± 0.062
4.879ValSer: 4.879 ± 0.12
3.67ValThr: 3.67 ± 0.202
4.161ValVal: 4.161 ± 0.121
0.388ValTrp: 0.388 ± 0.035
2.686ValTyr: 2.686 ± 0.102
0.0ValXaa: 0.0 ± 0.0
Trp
0.418TrpAla: 0.418 ± 0.023
0.068TrpCys: 0.068 ± 0.01
0.542TrpAsp: 0.542 ± 0.028
0.434TrpGlu: 0.434 ± 0.028
0.308TrpPhe: 0.308 ± 0.022
0.478TrpGly: 0.478 ± 0.027
0.089TrpHis: 0.089 ± 0.012
0.6TrpIle: 0.6 ± 0.035
0.431TrpLys: 0.431 ± 0.024
0.529TrpLeu: 0.529 ± 0.028
0.166TrpMet: 0.166 ± 0.015
0.509TrpAsn: 0.509 ± 0.047
0.142TrpPro: 0.142 ± 0.011
0.15TrpGln: 0.15 ± 0.013
0.222TrpArg: 0.222 ± 0.017
0.405TrpSer: 0.405 ± 0.024
0.424TrpThr: 0.424 ± 0.047
0.417TrpVal: 0.417 ± 0.025
0.089TrpTrp: 0.089 ± 0.011
0.296TrpTyr: 0.296 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.19TyrAla: 2.19 ± 0.058
0.563TyrCys: 0.563 ± 0.029
3.133TyrAsp: 3.133 ± 0.075
2.741TyrGlu: 2.741 ± 0.08
1.963TyrPhe: 1.963 ± 0.06
3.008TyrGly: 3.008 ± 0.081
0.651TyrHis: 0.651 ± 0.03
3.172TyrIle: 3.172 ± 0.08
2.732TyrLys: 2.732 ± 0.081
3.835TyrLeu: 3.835 ± 0.076
0.921TyrMet: 0.921 ± 0.035
3.099TyrAsn: 3.099 ± 0.126
1.354TyrPro: 1.354 ± 0.047
0.966TyrGln: 0.966 ± 0.039
1.206TyrArg: 1.206 ± 0.048
3.045TyrSer: 3.045 ± 0.087
2.197TyrThr: 2.197 ± 0.088
2.323TyrVal: 2.323 ± 0.063
0.362TyrTrp: 0.362 ± 0.028
1.973TyrTyr: 1.973 ± 0.068
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2209 proteins (760187 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski