Salinispira pacifica

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Salinispira

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5WCD2|V5WCD2_9SPIO Beta sliding clamp OS=Salinispira pacifica OX=1307761 GN=L21SP2_0002 PE=3 SV=1
MM1 pKa = 7.66VLDD4 pKa = 3.88FRR6 pKa = 11.84EE7 pKa = 5.1GYY9 pKa = 9.74ILQQIKK15 pKa = 9.7EE16 pKa = 4.37RR17 pKa = 11.84PVKK20 pKa = 10.19QYY22 pKa = 10.73QIHH25 pKa = 5.91EE26 pKa = 4.6KK27 pKa = 10.47IFILLLIPAAFLLASCDD44 pKa = 3.42FTGIDD49 pKa = 3.22NSAVLEE55 pKa = 4.12EE56 pKa = 4.94LAGDD60 pKa = 3.53WWLTDD65 pKa = 2.9VDD67 pKa = 4.79VYY69 pKa = 11.83ADD71 pKa = 3.72GQEE74 pKa = 4.36VTNSLPDD81 pKa = 3.37TLYY84 pKa = 10.29WPDD87 pKa = 3.21SVFAAEE93 pKa = 4.05GLEE96 pKa = 4.17YY97 pKa = 9.75PDD99 pKa = 4.88SVFITDD105 pKa = 3.74KK106 pKa = 10.79GWYY109 pKa = 8.97PSDD112 pKa = 3.45AYY114 pKa = 11.07YY115 pKa = 10.39PNDD118 pKa = 3.24AYY120 pKa = 11.55YY121 pKa = 9.43PDD123 pKa = 3.8SVFYY127 pKa = 10.71FPDD130 pKa = 3.34SVFFPDD136 pKa = 4.49SSFFPDD142 pKa = 3.55SFFFPDD148 pKa = 3.6SVFFPDD154 pKa = 4.71SVFQHH159 pKa = 6.48FPTRR163 pKa = 11.84MTVSEE168 pKa = 4.68DD169 pKa = 3.19GSVVVAADD177 pKa = 3.86IMWDD181 pKa = 3.13THH183 pKa = 6.8GFDD186 pKa = 4.32GSLGEE191 pKa = 4.39PGQISSEE198 pKa = 4.09PTIEE202 pKa = 3.93TSISVTEE209 pKa = 4.64DD210 pKa = 2.83GMLQTAGQNQVFTSPRR226 pKa = 11.84SGSRR230 pKa = 11.84NSGFSNWPKK239 pKa = 10.42NYY241 pKa = 9.89EE242 pKa = 3.64ITEE245 pKa = 4.22NGDD248 pKa = 3.95LILSKK253 pKa = 9.26TQTIDD258 pKa = 3.13GVEE261 pKa = 4.22YY262 pKa = 9.84TVEE265 pKa = 3.78EE266 pKa = 4.55HH267 pKa = 5.81FTRR270 pKa = 11.84EE271 pKa = 3.99APGAPDD277 pKa = 3.76FGGVLGWWEE286 pKa = 3.96YY287 pKa = 9.38TGKK290 pKa = 10.25EE291 pKa = 3.97SGMADD296 pKa = 3.94YY297 pKa = 11.09IHH299 pKa = 7.31ISPMDD304 pKa = 3.44IASSFGLIDD313 pKa = 3.46SGQSVVTGPLAGNIILWSYY332 pKa = 11.18SEE334 pKa = 4.38SDD336 pKa = 3.48STAGPSSGDD345 pKa = 3.34KK346 pKa = 11.06LLDD349 pKa = 3.98AGGLDD354 pKa = 3.48VGYY357 pKa = 9.77NAPDD361 pKa = 3.43GEE363 pKa = 4.65MILNQLSNTSPATSSSDD380 pKa = 2.61WTLNTFTWTYY390 pKa = 10.42SISGDD395 pKa = 3.37TLTIITGSTTLTYY408 pKa = 11.02DD409 pKa = 4.64KK410 pKa = 10.85IQAQQ414 pKa = 3.5

Molecular weight: 45.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5WJ47|V5WJ47_9SPIO Uncharacterized protein OS=Salinispira pacifica OX=1307761 GN=L21SP2_1816 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84IVRR5 pKa = 11.84SLIGRR10 pKa = 11.84AHH12 pKa = 7.73IGRR15 pKa = 11.84PSPGNIHH22 pKa = 6.3RR23 pKa = 11.84QPVRR27 pKa = 11.84NSMLNRR33 pKa = 11.84RR34 pKa = 11.84GAPGIFRR41 pKa = 4.43

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3350

0

3350

1098157

37

3663

327.8

36.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.693 ± 0.041

0.665 ± 0.013

5.722 ± 0.044

7.329 ± 0.047

4.358 ± 0.026

7.758 ± 0.051

2.097 ± 0.022

6.74 ± 0.035

4.124 ± 0.049

10.269 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.022

3.802 ± 0.032

4.473 ± 0.032

3.636 ± 0.028

6.464 ± 0.042

7.44 ± 0.036

4.523 ± 0.039

6.043 ± 0.042

1.081 ± 0.015

3.194 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski