Galdieria sulphuraria (Red alga)

Taxonomy: cellular organisms; Eukaryota; Rhodophyta; Bangiophyceae; Cyanidiales; Cyanidiaceae; Galdieria

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M2WZH5|M2WZH5_GALSU Pentatricopeptide (PPR) repeat-containing protein OS=Galdieria sulphuraria OX=130081 GN=Gasu_31260 PE=4 SV=1
MM1 pKa = 7.84WSTCMTHH8 pKa = 7.08AKK10 pKa = 9.92QPLSSTLIQKK20 pKa = 10.49DD21 pKa = 3.74YY22 pKa = 10.84FFKK25 pKa = 10.98LFSSSNQVVDD35 pKa = 4.55KK36 pKa = 11.39YY37 pKa = 10.99LFHH40 pKa = 6.94YY41 pKa = 9.9KK42 pKa = 10.25HH43 pKa = 6.6SCGEE47 pKa = 4.0LQRR50 pKa = 11.84VVLDD54 pKa = 3.74AWTVGCSSRR63 pKa = 11.84EE64 pKa = 3.66FSLKK68 pKa = 10.36NIRR71 pKa = 11.84SGSCYY76 pKa = 9.55PLKK79 pKa = 10.51PLLLVEE85 pKa = 5.35GGLSPEE91 pKa = 4.0TATFVVRR98 pKa = 11.84DD99 pKa = 3.96SLPYY103 pKa = 10.07KK104 pKa = 10.08QVLRR108 pKa = 11.84NTSQLFCQEE117 pKa = 4.02ANISVALSEE126 pKa = 4.77LSSSSLSDD134 pKa = 4.06DD135 pKa = 3.61EE136 pKa = 5.63SEE138 pKa = 4.72SGDD141 pKa = 3.41DD142 pKa = 4.22SSYY145 pKa = 11.54SDD147 pKa = 4.63SDD149 pKa = 3.68TDD151 pKa = 4.03SSNSTSDD158 pKa = 4.52DD159 pKa = 3.7DD160 pKa = 6.06ASDD163 pKa = 4.52SEE165 pKa = 4.49DD166 pKa = 4.04DD167 pKa = 3.95YY168 pKa = 11.96YY169 pKa = 11.76SYY171 pKa = 11.74DD172 pKa = 3.89EE173 pKa = 5.63GISDD177 pKa = 4.01YY178 pKa = 11.36DD179 pKa = 3.64SEE181 pKa = 4.39EE182 pKa = 4.61DD183 pKa = 3.74YY184 pKa = 11.14LYY186 pKa = 11.25SDD188 pKa = 4.72DD189 pKa = 5.61DD190 pKa = 3.93EE191 pKa = 5.47EE192 pKa = 5.36EE193 pKa = 4.23ILSEE197 pKa = 5.26SDD199 pKa = 5.04DD200 pKa = 4.2DD201 pKa = 5.83DD202 pKa = 3.94EE203 pKa = 4.97

Molecular weight: 22.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M2XRU0|M2XRU0_GALSU Uncharacterized protein OS=Galdieria sulphuraria OX=130081 GN=Gasu_59820 PE=4 SV=1
MM1 pKa = 7.92AILKK5 pKa = 10.1SRR7 pKa = 11.84SPLWLAQIGPLSPPIFPLRR26 pKa = 11.84NFPTLSHH33 pKa = 6.02SQFTHH38 pKa = 6.26SFPPHH43 pKa = 4.76TQNGNFQIKK52 pKa = 9.24IARR55 pKa = 11.84AHH57 pKa = 5.69SQSATLSKK65 pKa = 10.99LPIFPISRR73 pKa = 11.84KK74 pKa = 8.49NHH76 pKa = 4.71

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6602

433

7035

2960182

13

5016

420.8

47.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.588 ± 0.023

1.991 ± 0.014

4.82 ± 0.016

7.217 ± 0.029

4.827 ± 0.024

5.297 ± 0.023

2.292 ± 0.013

5.731 ± 0.022

5.924 ± 0.026

10.43 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.093 ± 0.011

4.301 ± 0.014

4.034 ± 0.023

4.308 ± 0.021

5.803 ± 0.021

8.983 ± 0.031

5.026 ± 0.017

6.499 ± 0.023

1.437 ± 0.012

3.305 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski