Vibrio phage JSF12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; Jesfedecavirus; Vibrio virus JSF12

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0YMF5|A0A2D0YMF5_9CAUD Uncharacterized protein OS=Vibrio phage JSF12 OX=1983595 PE=4 SV=1
MM1 pKa = 7.5IKK3 pKa = 10.2VEE5 pKa = 3.93NAIDD9 pKa = 3.89GKK11 pKa = 11.12KK12 pKa = 10.55GFTPIQITCSSGVPSEE28 pKa = 4.32CSNRR32 pKa = 11.84AYY34 pKa = 10.84LAIYY38 pKa = 9.6QDD40 pKa = 3.55NNAFAVFAADD50 pKa = 3.77EE51 pKa = 4.61DD52 pKa = 4.0EE53 pKa = 6.13LIIFDD58 pKa = 4.38ASSGNIDD65 pKa = 3.3YY66 pKa = 11.01AGNYY70 pKa = 9.3GDD72 pKa = 5.82LPDD75 pKa = 4.6NLVLTRR81 pKa = 11.84PVNCSITFTEE91 pKa = 4.24

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0YM57|A0A2D0YM57_9CAUD Uncharacterized protein OS=Vibrio phage JSF12 OX=1983595 PE=4 SV=1
MM1 pKa = 7.34TGKK4 pKa = 10.36KK5 pKa = 9.98LSARR9 pKa = 11.84TRR11 pKa = 11.84KK12 pKa = 9.54IEE14 pKa = 3.89GSFGFTLLEE23 pKa = 3.81ARR25 pKa = 11.84RR26 pKa = 11.84AGLTKK31 pKa = 10.66VLGLLSKK38 pKa = 10.25ATDD41 pKa = 4.5GYY43 pKa = 10.43VSKK46 pKa = 10.74HH47 pKa = 4.93PRR49 pKa = 11.84AVEE52 pKa = 3.78YY53 pKa = 8.49TASVFGLL60 pKa = 3.67

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

152

0

152

31360

37

1045

206.3

23.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.956 ± 0.389

1.25 ± 0.11

5.848 ± 0.145

6.942 ± 0.244

3.996 ± 0.151

6.18 ± 0.189

1.834 ± 0.127

5.768 ± 0.157

7.082 ± 0.198

9.075 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.388 ± 0.118

5.019 ± 0.187

3.476 ± 0.135

3.17 ± 0.127

4.503 ± 0.163

6.923 ± 0.286

5.979 ± 0.261

7.299 ± 0.239

1.199 ± 0.078

4.114 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski