Streptococcus phage Javan210

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AXL4|A0A4D6AXL4_9CAUD YopX domain-containing protein OS=Streptococcus phage Javan210 OX=2548048 GN=Javan210_0022 PE=4 SV=1
MM1 pKa = 7.45QYY3 pKa = 11.7GLFGDD8 pKa = 4.4FDD10 pKa = 5.37YY11 pKa = 9.56DD12 pKa = 3.37TWLSTHH18 pKa = 6.56EE19 pKa = 3.95DD20 pKa = 3.37HH21 pKa = 7.84EE22 pKa = 5.21EE23 pKa = 3.8IFQGDD28 pKa = 3.4EE29 pKa = 3.8DD30 pKa = 3.84EE31 pKa = 6.63AYY33 pKa = 10.53DD34 pKa = 3.56RR35 pKa = 11.84WKK37 pKa = 10.87DD38 pKa = 3.59EE39 pKa = 3.93QLEE42 pKa = 4.15DD43 pKa = 4.34EE44 pKa = 4.36EE45 pKa = 4.64WKK47 pKa = 10.58II48 pKa = 4.45

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AW17|A0A4D6AW17_9CAUD Uncharacterized protein OS=Streptococcus phage Javan210 OX=2548048 GN=Javan210_0021 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.15IEE4 pKa = 4.43LLHH7 pKa = 5.87VVNGHH12 pKa = 6.14RR13 pKa = 11.84KK14 pKa = 7.96FHH16 pKa = 6.76LGFFDD21 pKa = 6.0DD22 pKa = 3.34VHH24 pKa = 6.99QAIKK28 pKa = 10.53ALKK31 pKa = 7.78NHH33 pKa = 5.83VYY35 pKa = 9.92AYY37 pKa = 10.51SAISEE42 pKa = 4.07PRR44 pKa = 11.84FRR46 pKa = 11.84KK47 pKa = 10.17SMSGNSIRR55 pKa = 11.84IDD57 pKa = 3.58YY58 pKa = 8.68GAKK61 pKa = 7.16TCYY64 pKa = 10.28YY65 pKa = 9.83LLEE68 pKa = 4.24ARR70 pKa = 11.84KK71 pKa = 10.16VSS73 pKa = 3.35

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11580

42

1290

222.7

24.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.746 ± 1.02

0.449 ± 0.087

6.278 ± 0.373

6.822 ± 0.691

4.344 ± 0.167

6.874 ± 0.591

1.364 ± 0.182

6.434 ± 0.289

8.342 ± 0.512

7.34 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.268

6.14 ± 0.398

2.651 ± 0.155

4.335 ± 0.267

3.549 ± 0.324

6.33 ± 0.433

6.701 ± 0.404

6.468 ± 0.274

1.218 ± 0.135

4.136 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski