Vulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 / IC-017)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae; Vulcanisaeta; Vulcanisaeta distributa

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1QQF3|E1QQF3_VULDI 2'-5' RNA ligase OS=Vulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 / IC-017) OX=572478 GN=Vdis_1060 PE=4 SV=1
MM1 pKa = 7.78EE2 pKa = 6.34DD3 pKa = 3.63SIEE6 pKa = 4.22DD7 pKa = 3.77LLVGVEE13 pKa = 4.36DD14 pKa = 4.61GDD16 pKa = 3.93VEE18 pKa = 5.53SFMKK22 pKa = 10.9LIRR25 pKa = 11.84FVEE28 pKa = 4.0DD29 pKa = 2.98NYY31 pKa = 11.45RR32 pKa = 11.84KK33 pKa = 9.73VLYY36 pKa = 10.22TMGYY40 pKa = 9.52VEE42 pKa = 5.28LGDD45 pKa = 3.98YY46 pKa = 10.69ILIKK50 pKa = 10.34SCTYY54 pKa = 10.47ILLGSDD60 pKa = 3.17GMAYY64 pKa = 10.6ALLGGDD70 pKa = 3.13RR71 pKa = 11.84PEE73 pKa = 4.26VVNLEE78 pKa = 4.29TNGDD82 pKa = 3.5INEE85 pKa = 4.75VIDD88 pKa = 4.1EE89 pKa = 4.47VCGSGEE95 pKa = 4.0EE96 pKa = 4.26EE97 pKa = 4.03

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1QQW6|E1QQW6_VULDI Uncharacterized protein OS=Vulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 / IC-017) OX=572478 GN=Vdis_1149 PE=4 SV=1
MM1 pKa = 7.68GSRR4 pKa = 11.84IKK6 pKa = 10.01WLLVVLVVAVVVLFLVFAFRR26 pKa = 11.84VVSRR30 pKa = 11.84LGVRR34 pKa = 11.84SLVRR38 pKa = 11.84WVRR41 pKa = 11.84QALVPP46 pKa = 4.09

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2493

0

2493

708751

30

2515

284.3

31.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.061 ± 0.052

0.738 ± 0.019

4.846 ± 0.046

5.997 ± 0.055

3.447 ± 0.032

7.477 ± 0.045

1.448 ± 0.018

8.475 ± 0.054

4.636 ± 0.049

10.486 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.022

4.379 ± 0.044

4.492 ± 0.039

1.896 ± 0.026

6.369 ± 0.057

6.022 ± 0.045

4.837 ± 0.056

8.968 ± 0.051

1.286 ± 0.022

4.44 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski