Atopobacter sp. AH10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Atopobacter; unclassified Atopobacter

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1910 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A421BFE7|A0A421BFE7_9LACT Adenine DNA glycosylase OS=Atopobacter sp. AH10 OX=2315861 GN=mutY PE=3 SV=1
MM1 pKa = 7.4EE2 pKa = 4.72HH3 pKa = 5.61THH5 pKa = 6.46DD6 pKa = 4.52HH7 pKa = 5.71EE8 pKa = 6.26HH9 pKa = 7.11DD10 pKa = 4.42HH11 pKa = 6.87GLEE14 pKa = 4.11DD15 pKa = 3.77NKK17 pKa = 11.29DD18 pKa = 3.75FITIIDD24 pKa = 3.95EE25 pKa = 4.43NGDD28 pKa = 3.84EE29 pKa = 4.05QLHH32 pKa = 6.15TILLTFEE39 pKa = 4.61SDD41 pKa = 3.39DD42 pKa = 3.65FGRR45 pKa = 11.84SYY47 pKa = 11.47VITYY51 pKa = 9.61PSDD54 pKa = 3.41AEE56 pKa = 3.89NDD58 pKa = 3.56EE59 pKa = 4.38TVEE62 pKa = 4.08LSAFAYY68 pKa = 10.29EE69 pKa = 4.06EE70 pKa = 5.21DD71 pKa = 3.84EE72 pKa = 6.02DD73 pKa = 4.49GLQGTLMPIEE83 pKa = 4.87SDD85 pKa = 4.05EE86 pKa = 4.16EE87 pKa = 3.86WDD89 pKa = 3.91MVDD92 pKa = 3.35EE93 pKa = 4.27VLNTFMDD100 pKa = 4.39DD101 pKa = 3.37EE102 pKa = 4.45QLNSS106 pKa = 3.38

Molecular weight: 12.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A421BEQ5|A0A421BEQ5_9LACT L-threonine 3-dehydrogenase OS=Atopobacter sp. AH10 OX=2315861 GN=D3H64_07200 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 9.2KK9 pKa = 7.96RR10 pKa = 11.84HH11 pKa = 4.8RR12 pKa = 11.84QKK14 pKa = 10.16VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.72NGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 9.99SRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.62GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1910

0

1910

607519

26

4104

318.1

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.096 ± 0.071

0.859 ± 0.021

5.801 ± 0.063

7.141 ± 0.077

4.249 ± 0.046

6.54 ± 0.067

2.082 ± 0.026

7.191 ± 0.059

7.56 ± 0.084

10.359 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.036

4.193 ± 0.05

3.483 ± 0.042

3.937 ± 0.039

4.39 ± 0.048

6.283 ± 0.05

4.958 ± 0.05

6.615 ± 0.054

0.883 ± 0.016

3.812 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski