Amino acid dipepetide frequency for Cystobacter fuscus DSM 2262

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.099AlaAla: 13.099 ± 0.105	1.232AlaCys: 1.232 ± 0.021	4.8AlaAsp: 4.8 ± 0.043	7.323AlaGlu: 7.323 ± 0.071	3.626AlaPhe: 3.626 ± 0.037	8.942AlaGly: 8.942 ± 0.056	2.602AlaHis: 2.602 ± 0.032	3.299AlaIle: 3.299 ± 0.033	2.634AlaLys: 2.634 ± 0.031	14.414AlaLeu: 14.414 ± 0.108	2.272AlaMet: 2.272 ± 0.026	2.326AlaAsn: 2.326 ± 0.029	6.819AlaPro: 6.819 ± 0.066	4.21AlaGln: 4.21 ± 0.04	10.919AlaArg: 10.919 ± 0.079	6.891AlaSer: 6.891 ± 0.046	5.742AlaThr: 5.742 ± 0.053	7.989AlaVal: 7.989 ± 0.05	1.846AlaTrp: 1.846 ± 0.029	2.324AlaTyr: 2.324 ± 0.029	0.0AlaXaa: 0.0 ± 0.0
Cys	1.1CysAla: 1.1 ± 0.018	0.111CysCys: 0.111 ± 0.006	0.51CysAsp: 0.51 ± 0.017	0.572CysGlu: 0.572 ± 0.013	0.333CysPhe: 0.333 ± 0.01	0.999CysGly: 0.999 ± 0.026	0.273CysHis: 0.273 ± 0.01	0.306CysIle: 0.306 ± 0.009	0.194CysLys: 0.194 ± 0.008	0.926CysLeu: 0.926 ± 0.015	0.167CysMet: 0.167 ± 0.007	0.243CysAsn: 0.243 ± 0.011	0.585CysPro: 0.585 ± 0.014	0.33CysGln: 0.33 ± 0.009	0.649CysArg: 0.649 ± 0.017	0.584CysSer: 0.584 ± 0.015	0.558CysThr: 0.558 ± 0.015	0.697CysVal: 0.697 ± 0.015	0.135CysTrp: 0.135 ± 0.006	0.203CysTyr: 0.203 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	6.101AspAla: 6.101 ± 0.046	0.49AspCys: 0.49 ± 0.014	2.224AspAsp: 2.224 ± 0.032	3.374AspGlu: 3.374 ± 0.036	2.102AspPhe: 2.102 ± 0.023	4.789AspGly: 4.789 ± 0.055	0.87AspHis: 0.87 ± 0.017	1.867AspIle: 1.867 ± 0.025	1.45AspLys: 1.45 ± 0.023	4.71AspLeu: 4.71 ± 0.04	0.909AspMet: 0.909 ± 0.015	1.15AspAsn: 1.15 ± 0.019	2.946AspPro: 2.946 ± 0.032	1.247AspGln: 1.247 ± 0.018	2.969AspArg: 2.969 ± 0.032	2.51AspSer: 2.51 ± 0.029	2.645AspThr: 2.645 ± 0.029	4.194AspVal: 4.194 ± 0.037	0.831AspTrp: 0.831 ± 0.017	1.235AspTyr: 1.235 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	8.232GluAla: 8.232 ± 0.077	0.468GluCys: 0.468 ± 0.014	3.2GluAsp: 3.2 ± 0.032	4.685GluGlu: 4.685 ± 0.054	1.659GluPhe: 1.659 ± 0.021	5.632GluGly: 5.632 ± 0.038	1.594GluHis: 1.594 ± 0.024	1.717GluIle: 1.717 ± 0.024	2.094GluLys: 2.094 ± 0.033	7.71GluLeu: 7.71 ± 0.057	1.197GluMet: 1.197 ± 0.019	1.234GluAsn: 1.234 ± 0.021	3.866GluPro: 3.866 ± 0.038	2.997GluGln: 2.997 ± 0.033	6.671GluArg: 6.671 ± 0.069	3.313GluSer: 3.313 ± 0.032	2.8GluThr: 2.8 ± 0.028	5.201GluVal: 5.201 ± 0.039	0.854GluTrp: 0.854 ± 0.016	1.195GluTyr: 1.195 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.365PheAla: 3.365 ± 0.029	0.326PheCys: 0.326 ± 0.01	2.0PheAsp: 2.0 ± 0.026	2.179PheGlu: 2.179 ± 0.023	1.405PhePhe: 1.405 ± 0.023	2.819PheGly: 2.819 ± 0.027	0.84PheHis: 0.84 ± 0.016	1.293PheIle: 1.293 ± 0.02	0.93PheLys: 0.93 ± 0.017	3.491PheLeu: 3.491 ± 0.032	0.627PheMet: 0.627 ± 0.013	0.958PheAsn: 0.958 ± 0.019	1.649PhePro: 1.649 ± 0.023	1.276PheGln: 1.276 ± 0.019	2.219PheArg: 2.219 ± 0.027	2.346PheSer: 2.346 ± 0.028	2.116PheThr: 2.116 ± 0.027	2.488PheVal: 2.488 ± 0.029	0.459PheTrp: 0.459 ± 0.013	0.821PheTyr: 0.821 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	9.057GlyAla: 9.057 ± 0.061	1.004GlyCys: 1.004 ± 0.02	4.029GlyAsp: 4.029 ± 0.039	5.478GlyGlu: 5.478 ± 0.045	3.069GlyPhe: 3.069 ± 0.029	7.921GlyGly: 7.921 ± 0.08	2.019GlyHis: 2.019 ± 0.026	2.916GlyIle: 2.916 ± 0.028	2.907GlyLys: 2.907 ± 0.039	9.192GlyLeu: 9.192 ± 0.052	2.026GlyMet: 2.026 ± 0.024	2.127GlyAsn: 2.127 ± 0.033	4.233GlyPro: 4.233 ± 0.043	3.417GlyGln: 3.417 ± 0.035	6.73GlyArg: 6.73 ± 0.048	5.191GlySer: 5.191 ± 0.057	5.515GlyThr: 5.515 ± 0.055	6.747GlyVal: 6.747 ± 0.048	1.445GlyTrp: 1.445 ± 0.02	2.113GlyTyr: 2.113 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.489HisAla: 2.489 ± 0.029	0.229HisCys: 0.229 ± 0.007	1.026HisAsp: 1.026 ± 0.019	1.456HisGlu: 1.456 ± 0.022	0.875HisPhe: 0.875 ± 0.016	2.122HisGly: 2.122 ± 0.026	0.675HisHis: 0.675 ± 0.016	0.662HisIle: 0.662 ± 0.013	0.458HisLys: 0.458 ± 0.011	2.601HisLeu: 2.601 ± 0.031	0.375HisMet: 0.375 ± 0.011	0.394HisAsn: 0.394 ± 0.01	1.781HisPro: 1.781 ± 0.021	0.77HisGln: 0.77 ± 0.013	1.706HisArg: 1.706 ± 0.024	1.128HisSer: 1.128 ± 0.021	1.051HisThr: 1.051 ± 0.022	1.773HisVal: 1.773 ± 0.023	0.362HisTrp: 0.362 ± 0.011	0.593HisTyr: 0.593 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.503IleAla: 3.503 ± 0.035	0.282IleCys: 0.282 ± 0.008	1.835IleAsp: 1.835 ± 0.025	2.081IleGlu: 2.081 ± 0.027	0.978IlePhe: 0.978 ± 0.019	2.479IleGly: 2.479 ± 0.032	0.867IleHis: 0.867 ± 0.013	1.242IleIle: 1.242 ± 0.022	0.85IleLys: 0.85 ± 0.018	2.783IleLeu: 2.783 ± 0.033	0.37IleMet: 0.37 ± 0.011	0.939IleAsn: 0.939 ± 0.019	1.898IlePro: 1.898 ± 0.022	1.238IleGln: 1.238 ± 0.02	2.22IleArg: 2.22 ± 0.028	1.941IleSer: 1.941 ± 0.027	1.796IleThr: 1.796 ± 0.026	2.198IleVal: 2.198 ± 0.028	0.343IleTrp: 0.343 ± 0.009	0.697IleTyr: 0.697 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.258LysAla: 3.258 ± 0.038	0.202LysCys: 0.202 ± 0.008	1.6LysAsp: 1.6 ± 0.026	1.728LysGlu: 1.728 ± 0.027	0.649LysPhe: 0.649 ± 0.015	2.391LysGly: 2.391 ± 0.033	0.554LysHis: 0.554 ± 0.013	0.807LysIle: 0.807 ± 0.016	1.289LysLys: 1.289 ± 0.026	3.017LysLeu: 3.017 ± 0.035	0.601LysMet: 0.601 ± 0.014	0.8LysAsn: 0.8 ± 0.015	1.842LysPro: 1.842 ± 0.027	1.089LysGln: 1.089 ± 0.019	2.012LysArg: 2.012 ± 0.029	1.484LysSer: 1.484 ± 0.025	1.546LysThr: 1.546 ± 0.026	2.371LysVal: 2.371 ± 0.031	0.332LysTrp: 0.332 ± 0.011	0.589LysTyr: 0.589 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	13.61LeuAla: 13.61 ± 0.088	1.049LeuCys: 1.049 ± 0.018	5.576LeuAsp: 5.576 ± 0.046	7.967LeuGlu: 7.967 ± 0.06	3.587LeuPhe: 3.587 ± 0.033	9.761LeuGly: 9.761 ± 0.068	2.429LeuHis: 2.429 ± 0.029	3.073LeuIle: 3.073 ± 0.032	3.279LeuLys: 3.279 ± 0.036	12.561LeuLeu: 12.561 ± 0.1	1.986LeuMet: 1.986 ± 0.025	2.299LeuAsn: 2.299 ± 0.03	6.744LeuPro: 6.744 ± 0.047	3.432LeuGln: 3.432 ± 0.037	9.113LeuArg: 9.113 ± 0.06	7.432LeuSer: 7.432 ± 0.045	5.924LeuThr: 5.924 ± 0.041	8.743LeuVal: 8.743 ± 0.058	1.394LeuTrp: 1.394 ± 0.021	2.326LeuTyr: 2.326 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.283MetAla: 2.283 ± 0.027	0.125MetCys: 0.125 ± 0.007	0.991MetAsp: 0.991 ± 0.018	1.184MetGlu: 1.184 ± 0.018	0.485MetPhe: 0.485 ± 0.013	1.727MetGly: 1.727 ± 0.022	0.397MetHis: 0.397 ± 0.01	0.519MetIle: 0.519 ± 0.011	0.863MetLys: 0.863 ± 0.016	2.062MetLeu: 2.062 ± 0.026	0.445MetMet: 0.445 ± 0.012	0.631MetAsn: 0.631 ± 0.013	1.194MetPro: 1.194 ± 0.02	0.585MetGln: 0.585 ± 0.014	1.675MetArg: 1.675 ± 0.02	1.447MetSer: 1.447 ± 0.021	1.171MetThr: 1.171 ± 0.018	1.335MetVal: 1.335 ± 0.024	0.205MetTrp: 0.205 ± 0.007	0.298MetTyr: 0.298 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.548AsnAla: 2.548 ± 0.031	0.237AsnCys: 0.237 ± 0.009	1.061AsnAsp: 1.061 ± 0.022	1.19AsnGlu: 1.19 ± 0.019	0.752AsnPhe: 0.752 ± 0.017	2.172AsnGly: 2.172 ± 0.038	0.534AsnHis: 0.534 ± 0.013	0.871AsnIle: 0.871 ± 0.018	0.602AsnLys: 0.602 ± 0.014	2.375AsnLeu: 2.375 ± 0.033	0.375AsnMet: 0.375 ± 0.011	0.728AsnAsn: 0.728 ± 0.018	1.766AsnPro: 1.766 ± 0.026	0.786AsnGln: 0.786 ± 0.017	1.499AsnArg: 1.499 ± 0.019	1.17AsnSer: 1.17 ± 0.023	1.362AsnThr: 1.362 ± 0.024	1.777AsnVal: 1.777 ± 0.028	0.339AsnTrp: 0.339 ± 0.011	0.589AsnTyr: 0.589 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	6.699ProAla: 6.699 ± 0.062	0.423ProCys: 0.423 ± 0.01	3.238ProAsp: 3.238 ± 0.034	5.159ProGlu: 5.159 ± 0.045	1.981ProPhe: 1.981 ± 0.025	5.899ProGly: 5.899 ± 0.052	1.217ProHis: 1.217 ± 0.019	1.544ProIle: 1.544 ± 0.025	1.402ProLys: 1.402 ± 0.023	6.156ProLeu: 6.156 ± 0.047	1.257ProMet: 1.257 ± 0.019	1.212ProAsn: 1.212 ± 0.02	4.816ProPro: 4.816 ± 0.062	1.76ProGln: 1.76 ± 0.023	4.462ProArg: 4.462 ± 0.038	3.977ProSer: 3.977 ± 0.036	3.187ProThr: 3.187 ± 0.032	4.694ProVal: 4.694 ± 0.042	0.877ProTrp: 0.877 ± 0.017	1.15ProTyr: 1.15 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	4.288GlnAla: 4.288 ± 0.039	0.288GlnCys: 0.288 ± 0.011	1.538GlnAsp: 1.538 ± 0.02	2.26GlnGlu: 2.26 ± 0.027	0.898GlnPhe: 0.898 ± 0.017	3.278GlnGly: 3.278 ± 0.03	0.75GlnHis: 0.75 ± 0.015	0.884GlnIle: 0.884 ± 0.017	1.079GlnLys: 1.079 ± 0.018	3.918GlnLeu: 3.918 ± 0.038	0.684GlnMet: 0.684 ± 0.015	0.691GlnAsn: 0.691 ± 0.015	2.087GlnPro: 2.087 ± 0.028	1.485GlnGln: 1.485 ± 0.025	3.084GlnArg: 3.084 ± 0.03	1.78GlnSer: 1.78 ± 0.022	1.589GlnThr: 1.589 ± 0.019	3.195GlnVal: 3.195 ± 0.032	0.544GlnTrp: 0.544 ± 0.012	0.678GlnTyr: 0.678 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.254ArgAla: 9.254 ± 0.081	0.743ArgCys: 0.743 ± 0.016	3.763ArgAsp: 3.763 ± 0.036	6.09ArgGlu: 6.09 ± 0.053	3.117ArgPhe: 3.117 ± 0.027	6.118ArgGly: 6.118 ± 0.049	1.929ArgHis: 1.929 ± 0.025	2.918ArgIle: 2.918 ± 0.029	2.119ArgLys: 2.119 ± 0.028	9.569ArgLeu: 9.569 ± 0.057	2.086ArgMet: 2.086 ± 0.022	1.517ArgAsn: 1.517 ± 0.021	4.354ArgPro: 4.354 ± 0.04	2.925ArgGln: 2.925 ± 0.03	6.464ArgArg: 6.464 ± 0.052	3.958ArgSer: 3.958 ± 0.037	4.066ArgThr: 4.066 ± 0.035	6.576ArgVal: 6.576 ± 0.046	1.334ArgTrp: 1.334 ± 0.022	2.078ArgTyr: 2.078 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	6.753SerAla: 6.753 ± 0.055	0.568SerCys: 0.568 ± 0.015	2.559SerAsp: 2.559 ± 0.032	3.247SerGlu: 3.247 ± 0.028	2.242SerPhe: 2.242 ± 0.026	5.779SerGly: 5.779 ± 0.057	1.253SerHis: 1.253 ± 0.021	1.919SerIle: 1.919 ± 0.028	1.384SerLys: 1.384 ± 0.02	6.797SerLeu: 6.797 ± 0.048	1.148SerMet: 1.148 ± 0.018	1.397SerAsn: 1.397 ± 0.024	3.996SerPro: 3.996 ± 0.038	1.843SerGln: 1.843 ± 0.024	4.603SerArg: 4.603 ± 0.039	4.085SerSer: 4.085 ± 0.045	3.497SerThr: 3.497 ± 0.04	4.325SerVal: 4.325 ± 0.038	0.969SerTrp: 0.969 ± 0.018	1.264SerTyr: 1.264 ± 0.018	0.0SerXaa: 0.0 ± 0.0
Thr	5.567ThrAla: 5.567 ± 0.05	0.571ThrCys: 0.571 ± 0.019	2.414ThrAsp: 2.414 ± 0.032	2.835ThrGlu: 2.835 ± 0.029	1.993ThrPhe: 1.993 ± 0.024	5.063ThrGly: 5.063 ± 0.05	1.232ThrHis: 1.232 ± 0.02	1.387ThrIle: 1.387 ± 0.02	1.143ThrLys: 1.143 ± 0.018	6.613ThrLeu: 6.613 ± 0.045	0.781ThrMet: 0.781 ± 0.016	1.231ThrAsn: 1.231 ± 0.023	4.183ThrPro: 4.183 ± 0.047	1.904ThrGln: 1.904 ± 0.027	4.266ThrArg: 4.266 ± 0.033	3.258ThrSer: 3.258 ± 0.036	3.106ThrThr: 3.106 ± 0.037	4.344ThrVal: 4.344 ± 0.044	0.946ThrTrp: 0.946 ± 0.019	1.327ThrTyr: 1.327 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	8.382ValAla: 8.382 ± 0.055	0.763ValCys: 0.763 ± 0.016	4.294ValAsp: 4.294 ± 0.034	5.358ValGlu: 5.358 ± 0.043	2.475ValPhe: 2.475 ± 0.029	6.207ValGly: 6.207 ± 0.046	1.679ValHis: 1.679 ± 0.024	2.332ValIle: 2.332 ± 0.032	2.392ValLys: 2.392 ± 0.029	9.231ValLeu: 9.231 ± 0.061	1.513ValMet: 1.513 ± 0.023	1.761ValAsn: 1.761 ± 0.026	4.514ValPro: 4.514 ± 0.039	2.425ValGln: 2.425 ± 0.026	6.513ValArg: 6.513 ± 0.047	4.788ValSer: 4.788 ± 0.044	4.31ValThr: 4.31 ± 0.043	6.381ValVal: 6.381 ± 0.051	1.002ValTrp: 1.002 ± 0.02	1.613ValTyr: 1.613 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.403TrpAla: 1.403 ± 0.024	0.155TrpCys: 0.155 ± 0.006	0.69TrpAsp: 0.69 ± 0.016	0.816TrpGlu: 0.816 ± 0.016	0.508TrpPhe: 0.508 ± 0.012	1.137TrpGly: 1.137 ± 0.021	0.325TrpHis: 0.325 ± 0.01	0.392TrpIle: 0.392 ± 0.011	0.552TrpLys: 0.552 ± 0.013	1.781TrpLeu: 1.781 ± 0.026	0.414TrpMet: 0.414 ± 0.01	0.505TrpAsn: 0.505 ± 0.013	0.731TrpPro: 0.731 ± 0.016	0.453TrpGln: 0.453 ± 0.013	1.371TrpArg: 1.371 ± 0.024	1.048TrpSer: 1.048 ± 0.022	0.887TrpThr: 0.887 ± 0.017	1.146TrpVal: 1.146 ± 0.019	0.254TrpTrp: 0.254 ± 0.011	0.298TrpTyr: 0.298 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.318TyrAla: 2.318 ± 0.028	0.212TyrCys: 0.212 ± 0.008	1.262TyrAsp: 1.262 ± 0.025	1.337TyrGlu: 1.337 ± 0.021	0.876TyrPhe: 0.876 ± 0.019	1.903TyrGly: 1.903 ± 0.028	0.488TyrHis: 0.488 ± 0.012	0.593TyrIle: 0.593 ± 0.013	0.538TyrLys: 0.538 ± 0.013	2.378TyrLeu: 2.378 ± 0.027	0.386TyrMet: 0.386 ± 0.01	0.589TyrAsn: 0.589 ± 0.016	1.106TyrPro: 1.106 ± 0.018	0.828TyrGln: 0.828 ± 0.017	1.833TyrArg: 1.833 ± 0.026	1.295TyrSer: 1.295 ± 0.021	1.296TyrThr: 1.296 ± 0.023	1.755TyrVal: 1.755 ± 0.024	0.396TyrTrp: 0.396 ± 0.011	0.617TyrTyr: 0.617 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10511 proteins (3636354 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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