Streptomyces phage Nanodon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Likavirus; unclassified Likavirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1PAA2|A0A1B1PAA2_9CAUD BppL_N domain-containing protein OS=Streptomyces phage Nanodon OX=1873777 GN=SEA_NANODON_24 PE=4 SV=1
MM1 pKa = 7.75ALNDD5 pKa = 3.65AATLVVGSGNYY16 pKa = 7.74LTAPTGTAIPGDD28 pKa = 4.21LLAPASPWEE37 pKa = 4.34AVGHH41 pKa = 5.72TSLEE45 pKa = 4.38EE46 pKa = 3.81IFSIASEE53 pKa = 4.22GGEE56 pKa = 3.89ATVLGTLQNKK66 pKa = 7.6NLRR69 pKa = 11.84TKK71 pKa = 10.53YY72 pKa = 9.55SARR75 pKa = 11.84TEE77 pKa = 4.2TMTFTLQQFDD87 pKa = 3.49VDD89 pKa = 4.29GLKK92 pKa = 10.51LYY94 pKa = 10.68YY95 pKa = 10.14GANAPVLADD104 pKa = 3.41GTVGVPSDD112 pKa = 4.2PQPTVCSFLAVFVDD126 pKa = 4.13GDD128 pKa = 3.67NHH130 pKa = 6.41FAFYY134 pKa = 10.71APKK137 pKa = 10.65AEE139 pKa = 4.57IYY141 pKa = 10.38RR142 pKa = 11.84ADD144 pKa = 4.36DD145 pKa = 3.7LALSDD150 pKa = 4.1TEE152 pKa = 4.44SLAGLPLGVKK162 pKa = 10.15PMTHH166 pKa = 6.54NNNTWTYY173 pKa = 10.93AITPLGGGDD182 pKa = 3.17IAASGATAGTPGSFTPTGASAPANLAALQGSSIVASPATAWTTGQYY228 pKa = 10.78VDD230 pKa = 5.38LGDD233 pKa = 4.81GSDD236 pKa = 4.36AYY238 pKa = 10.06WDD240 pKa = 3.91GDD242 pKa = 3.29SWAAGQAVV250 pKa = 3.27

Molecular weight: 25.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1PA65|A0A1B1PA65_9CAUD DNA binding protein OS=Streptomyces phage Nanodon OX=1873777 GN=SEA_NANODON_47 PE=4 SV=1
MM1 pKa = 7.48TPPLPGRR8 pKa = 11.84PGVKK12 pKa = 9.97LAAIWEE18 pKa = 4.27ALTPAEE24 pKa = 4.05RR25 pKa = 11.84QLFKK29 pKa = 10.71PVLLGSSSAEE39 pKa = 3.67WLADD43 pKa = 3.41TLRR46 pKa = 11.84SSGHH50 pKa = 5.5EE51 pKa = 3.96VSASTIRR58 pKa = 11.84TYY60 pKa = 10.92RR61 pKa = 11.84RR62 pKa = 11.84ALRR65 pKa = 11.84RR66 pKa = 11.84EE67 pKa = 4.16GAVSVV72 pKa = 3.83

Molecular weight: 7.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15432

35

1159

205.8

22.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.562 ± 0.303

0.739 ± 0.109

6.383 ± 0.21

7.206 ± 0.518

3.104 ± 0.171

8.612 ± 0.409

2.054 ± 0.187

4.575 ± 0.328

4.374 ± 0.258

8.294 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.048 ± 0.148

3.149 ± 0.147

4.834 ± 0.199

3.266 ± 0.15

6.344 ± 0.403

5.923 ± 0.267

6.532 ± 0.416

7.309 ± 0.232

1.86 ± 0.136

2.832 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski